Effect of IBA and Bacteria (Agrobacterium rubi ve Bacillus OSU 142) on the Rooting of M9 Apple Rootstock Cuttings

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Title

Effect of IBA and Bacteria (Agrobacterium rubi ve Bacillus OSU 142) on the Rooting of M9 Apple Rootstock Cuttings

Author

Pirlak, Lütfi
Baykal, Yüksel

Abstract

This study was carried out to investigate the effects of IBA and bacteria (Agrobacterium rubi ve Bacillus OSU 142) applications on the rooting of M9 rootstock cuttings. The cuttings treated with IBA alone at different concentrations (2000, 4000 and 6000 ppm) and in combinations of three Agrobacterium rubi (A1, A16, A18) and Basillus OSU 142 strains. The treated cuttings were placed mist propagation system including perlite medium. After three months the cuttings were uprooted and evaluated for rooting percentage (%), callusing (%) and viability rate (%). There were determined inadequate results about rooting of cuttings. Rooting in cuttings was observed in 2000 ppm IBA (6.66 %), 4000 ppm IBA (13.33 %), and 2000 ppm IBA + Basillus OSU 142 combination (6.66 %), respectively. Callus were observed in all treated. Callus rate was 66.66 % in control while the rate was 84.61 % in A16 + A18 applications. Callus rate decreased depending on increasing of IBA doses. There were determined significantly differences in bacteria alone applications. According to application of bacteria combinations, the highests callus rate was A16 + A18 while the lowest was A1 + OSU 142. The best result of hormones + bacteria combinations were taken from 4000 ppm IBA + A18 application. Cutting living rate was similar to callus rating results. Many of the cuttings were live after application. Living rate was low at alone IBA application compared to that of the control.

Keywords

Conference or Workshop Item
PeerReviewed

Date

2009-06

Extent

492

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