Biodiversity for sustinable agriculture: Common bean genetic diversity

Biodiversity for sustinable agriculture: Common bean genetic diversity

Nemli , Seda

The immense genetic diversity of genotypes of crops is the most directly useful and economically valuable part of biodiversity. Genetic diversity is a key factor enabling adaptation, and therefore survival, of natural populations in changing environments. And also genetic diversity is essential tool for any breeding program. Leguminous plants, after cereals, include the most economically important species of agricultural interest, considering area cultivated and total production. Among the grain legumes,soybean, peanuts and common beans are the most important commercial crops. Common bean (Phaseolus vulgaris L.) and its related species are important protein sources for the world population. In 2006, the bean industry was valued at $1.2 billion and $180 million in USA and Canada, respectively. The average yield of bean varieties cropped in developing countries is still very low. The analysis of genetic diversity and relationships among different individuals, species, or populations is an important topic in genetics and plant breeding. Since morphological charactersin plants effect from environmental condition, DNA markers provide the most precise tool for measuring genetic relationships, because they are potentially unlimited in number Among the DNA techniques, Amplified Fragment Length Polymorphism (AFLP) is intense and provides a powerful tool for genotype identification, phylogeny The AFLP technique is based on the amplification of short restriction endonuclease digested genomic DNA fragments onto which adaptors have been ligated at both ends.For this purpose common bean genomes were analyzed using AFLP fingerprinting to examine the genetic variation within and among genotypes.. A total of 86 common bean accessions collected from different countries were used in this study. For the AFLP analysis,12 primer combination were used. Acrylamide gels from primer combination were scored according to presence (1) or absence (0) of amplified fragments.The molecular data were analyzed using the NTSYs program. A dendrogram was generated using JMP software (version 3.1, SAS Institute, 1995) based on the UPGMA (unweighted pair-group method of arithmetic average). The eightysix genotypes represented seven different clusters as revealed by AFLP primers. The minimum variation was detected between sample 20, Turkey and sample 24, Turkey (GD = 0.09), and the maximum was found between samples 34 and 28 (GD = 0.80). Keywords: Biodiversity, Common bean, AFLP

Conference or Workshop Item
PeerReviewed

2012-05-31

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