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                    <text>Ectoparasitic Diseases in Freshwater Ornamental Fish and Their
Treatments
C.Erkin Koyuncu
Department of Aquaculture, Faculty of Fisheries
Mersin University TURKEY
ekoyuncu@mersin.edu.tr
Erol Tokşen
Department of Aquaculture, Faculty of Fisheries
Ege University TURKEY
erol.tokşen@ege.edu.tr
Abstract: Fish parasites and their effects have become increasingly visible during the latest
decades inconnection with the development of fresh water ornamental Fish industries
troughout the world. Diseases problem including hazards caused by parasitic organisms are
the main threat to further increase of the industry. Ectoparasites are the most common and
widely distributed of freshwater ornamental Fish. Such as, protozoan ectoparasites of
aquarium fish (Ichthyophthirius multifiliis, Ichthyobodo necatrix, Chilodonella cyprini,
Oodinium limneticum, Trichodinids); external worms of fish (Dactylogyrus extensus,
Gyrodactylus bullutarudis); external crustaceans (parasitic copepods, Argulus japonicus,
Argulus foliaceus, Lernaea cyprinacea).The fish louse Argulus spp. is now the main problem
in cage-cultured freshwater ornamental Fish in the fresh water environment in Europa
countries. Gyrodactylus bullatarudis had caused the mortality in guppy ornamental fish.
White Spot Disease (Ichthyophthiriosis) occurs in ornamental fish fry interprise caused the
considerable economic loss. Ornamental fish were affected heavily by ectoparasites due to the
very fine structure of the skin. Ectoparasites causing in ornamental fish only kills the fish but
also reduces the market value of fish. The present work aim to the parasitic diseases of
freshwater ornamental fish, how they are transmitted, which effects they have on ornamental
fish, how they could be diagnosed, and how they could be controlled and treated.

Introduction
Fish parasites and their effects have become increasingly visible during the latest decades inconnection
with the development of freshwater ornamental Fish industries troughout the world. Diseases caused by parasites
are widespread and cause loses of fish in intensively stocked pond and aguarium. Ectoparasites of freshwater
ornamental fish come in all sizes and shapes and inculude single-celled protozoan, and mullticellular trematodes
(flatworms), crustaceans and artropods (Roberts 2010). Parasites can infest the outer surface or penetrate the
paranchyma of almost any tissue of the host. Fish can serve as an intermediate, paratenic (transport) or definetive
host for various stages of parasites. Ectoparasitic infections in freshwater ornamentals fishes are diagnosed by
wet mount cytology preparations of skin scrapes, gills biopsies, and by direct observation (macroscopic
parasites) (Woo 2006, Roberts 2010). Ectoparasites are the most common and widely distributed of freshwater
ornamental Fish (Tab.1). These parasites, in crowded pools and aquariums, together with increasing water
temperature when appropriate conditions are found to cause large losses. Ornamental fish were affected heavily
by ectoparasites due to the very fine structure of the skin. Ectoparasites causing in ornamental fish only kills the
fish but also reduces the market value of fish (Mousavi 2003,Tokşen 2006, Koyuncu 2009). In this review,
treatment and control of ectoparasites of freshwater ornamental fish in the recent developments were reviewed.

The Study
Research Significance
In this study, ectoparasite of freshwater ornamental diseases and drug therapy are discussed.
Ectoparasite freshwater ornamental that can be used in treatment of diseases and drugs are defined and explained
the general features.

683

�Important Fish Ectoparasite Groups Caused Loses in Ornamental Fish
In this section, the systematic groups that represent the most important examples are chosen.
Protozoa:
Protozoans are the most common ectoparasites encountered in ornamental fish. Although some authors
consider them harmless, many serious fish losses are caused by protozoan ectoparasites (Krier and Baker 1987
Durborow et al.1998, Scholz 1999, Wildgoose 2001). Protozoans vary in shape and size and live mainly on the
gills, fins, and skin of fish.
There are a number of protozoan ectoparasites long recognized as causative agents of severe diseases
such as flagellates of the genus Oodinium sp, or Ichthyobodo sp. and the cilli protozoan ectoparasites,
Ichthyophthirius multifiliis, Chilodonella sp. Trichodina sp. are some of the most significant pathogens in
ornamental fish (Tab. 1). (Durborow 2003).
Oodinium sp. is a problem in freshwater ornamental fishes. Most reports of the parasite have been on aquarium
fishes.( Lom et.al. 1983).
Ichthyobodo sp. - formerly (and still commonly) called Costia. A flagellated protozoal ectoparasite. A
normal inhabitant of fish skin. Poor water quality and other stresses (especially crowding) may allow this
normally mutualistic parasite to reproduce rapidly and overwhelm the host. Microscopically the protozoa are
very small (5-10 microns), move rapidly, and are shaped like small sickles. They may be attached to host tissue
or swimming free. Most common in freshwater species of fishes (Joyon et al., 1969).
Ichthyophthirius multifiliis - known commonly simply as "Ich." The largest protozoal parasite of fish
and one of the most commonly encountered. Trophozoites may reach 1.0 mm in diameter. This interference will
be placed into the skin. Protection against other pathogens in patients with low-grade infection destroys the
system. Whereas in cases of severe infections can cause death quickly. Excessive growth of cysts on the pool
floor and as such is a suitable environment for this group is very high virulence of the parasite infection
In particular, in the ornamental fish Ichthyophthiriosis loss caused millions of measured by dollars (Durborow
et.al., 1998).
Chilodonella sp. - A ciliated protozoan which can cause high morbidity and mortality among freshwater
tropical fishes at the wholesale and fish farming levels of the industry. Attacks skin and gills. Easily identified
microscopically by its heart-shaped structure and slow circular motion when not crawling on the surface of the
fish ( Koyuncu, 2003).
Trichodina sp.- A disc-shaped ciliate protozoan found on the skin and gills of many freshwater fish.
Circular rows of denticles and a ciliary girdle give this parasite a unique radial symmetry. Probably not harmful
when present in small numbers (Ozer et al., 1998).
Monogenean Platyhelminthes:
Monogeneans are parasitic flatworms or flukes with direct live cycle that infest the external surfaces of
almost any species of ornamental fish. The mongeneans have an anterior oral sucker used for feding on mucus
and sloughed epithelial cells, while the posterior end has a organ for attaching to host. These parasites cause
focal irritation, increased mucus production, and hyperplasia of the epithelial tissues, and open a portal for
secondary bacterial and fungal infections. Severe infections can cause erratic swimming behavior, ’flashing’
respiratory activity, scattered hemorrhages with epithelial ulceration and frayed fin. Monogenea species the
economic importance of fish in the severe loss causes: Common genera found in ornemantal fish inculude:
Dactylogyrus sp. and Gyrodactylus sp. Fancy gold fish are commonly infected with ‘gill’ flukes, Dactylogyrus
extensus, while Gyrodactylus katherineri skin flukes infestastion are more often observed in koi. Gyrodactylus
bullatarusdis and Gyrodactylus Turnbull are guppy fish flukes (Tab. 1). (Woo 2006, Roberts2010).
Arthropoda (Crustacea):
Crustasea s play an important role in fish parasites is a group. There are a number of crustacean
parasites that infect the skin and gills of tropical and ornamental fish (Tab. 1), Lernaea sp. or ‘ anchor form’ is a
copepod crustacean of pond-reared fish, especially gold fish, carp, koi and guupy. The infections larval stage of
this particular parasite penetrates the skin of the fish and continues to develop. There is usually an intense focal
inflammatory reaction at the site of penetration, which often results in hiperplasia of tissue around the site of
parasites development( Roberts , 2010)
Ergasilus sp. is a species of another type of copepod parasite. The parasites are most commonly found
attached to the gill filaments of many species of pond and ornamental fish. (Robert, 2010)
684

�The ‘fish louse’, Argulus sp. is a common branchiurid crustacean parasite of many species of pond and
ornamental fish. This parasite carwls over the surface of the fish and uses its stylet to pierce the outer epithelial
cells of the fish and ingest the cell’s contents. There is a severe inflammatory reaction at the site of stylet
penetration, sugesstion that a substance is released by the parasite to facilitate feeding. Because of the this feding
activity this parasite has also been imlicated in the mechanical transmission of several bacterial, viral and
hemoprotozoal diseases (Toksen, 2006, Robert, 2010) The fish louse Argulus sp. is now the main problem in
cage-cultured freshwater ornamental Fish in the fresh water environment in Europa countries (Woo, 2006).
Way of
Parasites
Size
Host
Position
Location
transsimition
Protozoa:
Flagella
Oodinium sp.

12-90 µm

Freshwater
ornamentals
fish

Skin

Of floating
phase
skin invasion

Icthyobodo sp.

5-18 µm

Freshwater
ornamentals
fish

Skin

Of floating
phase
skin invasion

Ichthyophthirius
multifiliis

50-1000µm
(trophozoites)

Freshwater
ornamentals
fish

Skin,
Epithelial
tissues

Trichodina sp.

35-60 µm

Freshwater
ornamentals
fish

Skin and gills

Chilodonella sp.

30-80 µm

Freshwater
ornamentals
fish

Skin and gills

Of floating
theront
invasion
Of floating
phase
skin and gills
invasion
Of floating
phase
skin and gills
invasion

Ciliate

Monogenea:
Gyrodactylus sp

350-460 µm

Dactylogyrus sp.

990-1584 µm

Freshwater
ornamentals
fish
Freshwater
ornamentals
fish

Skin and fin

Body contact

Gills and skin

Body contact

Arthropoda
Lernaea sp.

Ergasilus sp.

Argulus sp.

Freshwater
5-20 mm
ornamentals
Skin and fin
Body contact
fish
Freshwater
1-2 mm
ornamentals
Gills
Body contact
fish
Freshwater
8-13 mm
ornamentals
Skin and fin
Body contact
fish
Table 1. Common Ornamental Fish Ectoparasites

685

�Medicaments Used in Treatment of Freshwater Ornamental Parasites:
The applied treatments for diseases are prevention and good health management. However,
Chloramines-T Formaldehyde, Potassium permanganate, Acetic acid, Copper sulfate, Malachite green and salt
are commonly used to control protozoan fish ectoparasites (Tab. 2). Salt, formaldehyde, and vinegar appeared to
be the most effective chemicals to treat protozoan infestation (Stoskopf, 1993, Noga, 2001, Timur et. al ,2003
Kayis et. al,. 2005, Balta et al., 2008, Dörücü et.al., 2008, Kayis et al., 2009). Levamisol, Mebendazole
Triclorphon and formalin are commonly used to control treat metazoan parasites (Lasee, 1995, Toksen, 2006).
Among the chemicals that are used to treat or prevent parasitic fish diseases in Turkey, Acetic acid, Betadine,
Chloramin-T, Copper sulfate, Formalin, Hydrogen peroxide, Malachite green, Levamisol, Mebendazole,
Potassium permanganate and salt are authorized by the European Union by the council regulation (EEC) no.
2377/90 of the European Council.
In most countries, very few drugs and chemicals have been registered for treatment of food fish. Indeed,
many biocides (e.g., malachite green) are banned from use in most countries and severe measures are taken
against exporters of fish and shellfish that contain residues. Due to the carcinogenic and genotoxic potentials of
malachite green, it has been prohibited for use in the production of consumer fish in the European Union by
regulation no. 2377/90 of the European Council. Drugs and chemicals used to treat fish must be safe to the fish
and the environment, as well as to human.
Used for the control of freshwater ornamental ectoparasites in the market are several chemical
substances. These chemicals in general are also used in other hosts. Metabolism of fish is different, the effects of
these substances in freshwater ornamenta ectoparasites is weak. Therefore, the fish farms to prevent excessive
loss of fish to specific research and development antiparazit compounds are needed. Toltrazuril similar drugs are
promising for broad spectra (Tab. 2). (Dörücü et.al , 2008).
Antiparasitic agent
Chloramin-T*

Chemical Dosage;time
7-15 mg/l; 1 h

Formaldehyde*

0.167-0.25 mg/l; 1 h
0.25 mg/l; indefinite
250-500 mg/l; 30-60 min
0.5 mg/l
1-2 mg/l; 1-10 min
50 mg/l; 30 min
0.1-0.15 ppm/12-24 h
50 ml/l; 2 h
1 mg/l; 24 h
4ml(1000 ml water)
13.5 ppm for several days
10 ppm for sevaral days
2-5 mg/lt 1h

Hydrogen peroxide*
Copper sulfate*
Acetic acid*
Betadine*
Malachite green*
Levamisol*
Mebendazole
Toltrazuril
Qunine hydrochloride
Atebrine
Potassium
permanganate*
Dimilin
Triclorphon

Ectoparasite
Protozoan, monogenetic
trematodes
External parasites

Treatment
Bath treatment

External parasites
External parasites
External parasites
External parasites
External parasites
Monogenetic trematodes
Monogenetic trematodes
Monogenetic trematodes
Artropoda ectoparasites
Artropoda ectoparasites
Artropoda ectoparasites

Bath treatment
Bath treatment
Bath treatment
Bath treatment
Bath treatment
Bath treatment
Bath treatment
Bath treatment
Bath treatment
Bath treatment
Dip treatment

Bath treatment

0.01 mg/lt
Artropoda ectoparasites
Bath treatment
0.25-5 ppm for several
Artropoda ectoparasites
Bath treatment
hours
DTHP
2.5 ppm 1hour
Artropoda ectoparasites
Bath treatment
Salt*
3% solution; 15-30 min
External parasites
Bath treatment
0.5% solution; indefinite
* Chemicals authorized by council regulation (EEC) no. 2377/90 of the European Council
Table 2. Control and Treatment of Ectoparasitic Diseases in Freshwater Ornamental Fish

686

�Conclusions
Hundreds of fish parasites in their natural environment type has been found infected, although rarely
leads to death of fish. In tropical fish culture reduces the number of common parasites, but they do influence is
great. Parasites of fish death, loss of appetite, the slowdown in growth, deterioration of reproductive ability,
reduce resistance to other pathogens, and cause marketing with unpleasant views. Despite these negative effects
on the market for the treatment of fish parasites in a small number of drugs are used. Of this review, the
treatment of diseases in tropical fish culture, fish ectoparasites shed light manufacturers believe.

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CABI Publ.

688

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Toksen, Erol</text>
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                <text>Fish parasites and their effects have become increasingly visible during the latest  decades inconnection with the development of fresh water ornamental Fish industries  troughout the world. Diseases problem including hazards caused by parasitic organisms are  the main threat to further increase of the industry. Ectoparasites are the most common and  widely distributed of freshwater ornamental Fish. Such as, protozoan ectoparasites of  aquarium fish (Ichthyophthirius multifiliis, Ichthyobodo necatrix, Chilodonella cyprini,  Oodinium limneticum, Trichodinids); external worms of fish (Dactylogyrus extensus,  Gyrodactylus bullutarudis); external crustaceans (parasitic copepods, Argulus japonicus,  Argulus foliaceus, Lernaea cyprinacea).The fish louse Argulus spp. is now the main problem  in cage-cultured freshwater ornamental Fish in the fresh water environment in Europa  countries. Gyrodactylus bullatarudis had caused the mortality in guppy ornamental fish.  White Spot Disease (Ichthyophthiriosis) occurs in ornamental fish fry interprise caused the  considerable economic loss. Ornamental fish were affected heavily by ectoparasites due to the  very fine structure of the skin. Ectoparasites causing in ornamental fish only kills the fish but  also reduces the market value of fish. The present work aim to the parasitic diseases of  freshwater ornamental fish, how they are transmitted, which effects they have on ornamental  fish, how they could be diagnosed, and how they could be controlled and treated.</text>
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                    <text>Recent Developments On The Aplication Of Artemia
In The Ornamental Fish Culture
Edis Koru
Ege University, Fisheries Faculty, Department of Aquaculture, Bornova-Izmir/Turkey
edis.koru@ege.edu.tr
Gurel Turkmen
Ege University, Fisheries Faculty, Department of Aquaculture, Bornova-Izmir/Turkey
gurel.turkmen@ege.edu.tr
Abstract: Production of animals for the aquarium hobbyist trade is a rapidly growing sector
of the aquacultural industry, and it will continue to become more important as restrictions are
placed on collecting animals for the wild. Improved techniques for marine food-fish
larviculture since the early 1980’s have greatly enhanced the growth and survival of
freshwater ornamental fish larvae largely through improved technology regarding live food
culture and larval rearing practices. Research developments in larviculture and early rearing
technology have allowed 90% of currently marketed freshwater ornamental fish to be cultured.
However, for marine ornamentals, the reverse is true as only a handful of species is produced
via aquaculture technology. A major task in devising a protocol for the artificial propagation
of a fish species is the development of a feeding regimen for the larvae. Live feeds are a
convenient and often essential food source for the larvae of some cultured species, especially
those without a fully developed digestive system. In such cases, live food organisms provide
digestive enzymes that breakdown the food ingested by larvae and can be described as
naturally encapsulated bags of nutrients. Two major concerns among aquaculturists are
providing organisms appropriate to the size of the larvae at the first feeding stage and then
supplying the large numbers of feed organisms necessary to maintain the larvae. Since no
artificial feed formulation is yet available to completely substitute for Artemia, feeding live
prey to young fish larvae still remains essential in commercial hatchery operations. This paper
reports the recent developments in the applications of Artemia nauplii, decapsulated Artemia
cysts and on-grown Artemia in the ornamental fish culture.
Key words: Artemia, Ornamental Fish, Larvae, Feeding

1. Introduction
The ornamental fish sector is a widespread and global component of international trade, fisheries,
aquaculture and socio-economic development. Since 1985, the international trade in exports of ornamentals,
which usually takes place in the majority of developing countries, followed an increasing trend with an average
growth rate of approximately 14% per year. The entire industry has been estimated to be worth around US$15
billion. This vast industry has the potential to contribute to the economic growth of developing countries which
may face future challenges regarding environmental safety (Olivotto et al., 2006). Production of animals for the
aquarium hobbyist trade is a rapidly growing sector of the aquacultural industry, and it will continue to become
more important as restrictions are placed on collecting animals for the wild. Currently, approximately 90% of
freshwater fish traded in the hobbyist industry are captively cultured. While a majority of aquacultural
production worldwide is devoted to food production, ornamental fish production is an important component of
the aquaculture industry in several nations. In Singapore, ornamental fish accounts for 40% of their total exports.
In the United States, ornamental fish production is the fourth largest sector behind catfish, trout, and salmon.
Farms in Florida produce 800 varieties of freshwater fish (Tlusty, 2002).
Successful rearing of larval stages of aquatic organisms is a challenge for aquarium hobbyists, an aim
and a necessity for the success of the aquaculturist. All these specialists will agree that the primary problem in
any type of larval rearing is that of food. Ideally, one would prefer to feed larvae their natural diet, which is
characterized by a wide diversity of nutritious live organisms. Live feed is an essential food source for the fry of
cultured species, especially those without a fully developed digestive system. In the freshwater ornamental fish
culture, Artemia nauplii are used as the live feed. Two major concerns of aquaculturists are: (i) providing
organisms appropriate to the size of the feed to the first feeding stage and (ii) supplying adequate number of feed
organisms to ensure higher survival and faster growth (Arulvasu and Munuswamy, 2009). In nature, zooplankton

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�is one of the primary foods of larval fish. The brine shrimp Artemia is in the phylum Arthropoda, (Crustacea,
Anostraca). Artemia spp., are zooplankton, like copepods and Daphnia, which are used as live food in the
aquarium trade, and for freswater and marine fish larval culture and crustacean larval culture (Lim et.al., 2001).
While the adult form of Artemia is primarily used as a live, frozen, or freeze-dried food in the aquarium trade,
the nauplius stage is used exclusively in fish hatchery operations. It was recognized long ago that freshly hatched
Artemia nauplii are a high value feed for fish larvae and fry. Because of the size of the nauplius stage, Artemia
also represent the only practical food source for the early stages of many fish and crustacean larvae. (Tamaru et
al., 2001). In Singapore, the top-exporting country of freshwater ornamental fish in the world, Moina used to be
the most common live food organism used in the industry. As Moina is cultured in ponds using pig waste, they
are often contaminated with fish pathogens, as well as bacteria of public health concern, such as Salmonella and
Vibrio cholera. To minimize the risk of fish being contaminated with the pathogens, more and more freshwater
ornamental fish farmers in Singapore have shifted from Moina to the cleaner Artemia nauplii for feeding their
fish. (Lim et al,. 2002, 2003). Since no artificial feed formulation is yet available to completely substitute for
Artemia, feeding live prey to young fish larvae still remains essential in commercial hatchery operations. There
are more than 50 geographical strains of Artemia. Many commercial harvesters and distributors sell brands of
various qualities. This paper reports the recent developments in the applications of Artemia nauplii, decapsulated
Artemia cysts and on-grown Artemia in the ornamental fish culture.
1.1. Why is Live Feed Necessary?
Fish biologists categories larvae of two types: precocial and altricial. Precocial larvae are those that,
when the yolk sac is exhausted, appear as mini-adults, exhibiting fully developed fins and a mature digestive
system including a functional stomach. Such fish can ingest and digest formulated diets as a first food and are
best exemplified by the salmon and trout raised extensively in hatcheries around the world without the benefit of
live food. Altricial larvae are those that, when the yolk sac is exhausted, remain in a relatively undeveloped state.
The digestive system is still rudimentary, lacking a stomach, and much of the protein digestion takes place in
hindgut epithelial cells (Govoni et al., 1986). Such a digestive system seems (at this point) to be incapable of
processing formulated diets in a manner that allows survival and growth of the larvae comparable to those fed on
live feed. Altricial larvae therefore appear to require live feed, but there may be other reasons besides the
digestibility question. Live feeds are able to swim in the water column and are thus constantly available to the
larvae. Formulated diets tend to aggregate on the water surface or, more commonly, sink quickly to the bottom,
and are thus normally less available to the larvae than are the live feeds. In addition, the movement of live feed
in the water is likely to stimulate larval feeding responses, since evolutionary history has probably adapted them
to attack moving prey in nature. Formulated diets are generally capable of moving only in a downward direction,
towards the bottom. Finally, live prey, with a thin exoskeleton and high water content, may be more palatable to
the larvae once taken into the mouth, compared with the hard, dry formulated diets. This last point is rather
critical, especially when considered in light of the fish larva’s absence of feeding appendages; any foods must
enter the mouth whole (i.e. the larva’s mouth gape must be of sufficient size for particle ingestion to occur) and
they are quickly either accepted or rejected on the basis of palatability (Stottrup and McEvoy, 2003).

2. Artemia
Artemia has several characteristics which make it ideal for aquaculture use. It is easy to handle,
adaptable to a wide-range of environmental conditions, non-selective as a filter-feeder which can ingest algae,
protozoa and bacteria of the correct size (10–50 µm) and is capable of growing at very high densities (Landau et
al. 1985; Lèger et al. 1989). Artemia also has a high nutritive value (40–60 percent protein, rich amino acid
composition), an unchanging food requirement, high conversion efficiency, short generation time, high fecundity
rate and long lifespan. The whole animal (even adult stage) may be consumed without previous processing by
many aquaculture organisms. In the food chain the nutritional value of Artemia depends on both the
macronutrients (proteins, fats and carbohydrates) and micronutrients (vitamins and minerals) it can accumulate
from the filtered food. The brine shrimp is considered a continuous, non-selective, obligate phagotrophic filter
feeder zooplankton (Fig.1).

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�Figure 1. Adults Artemia sp.
Artemia are extremely euryhaline, withstanding salinities from 3 ppt to 300 ppt. They can even survive
short periods of time in freshwater, but cannot reproduce in it. Artemia survive temperatures ranging from 15 to
55 oC. They have two modes of reproduction. Sometimes nauplii (first Artemia swimming stage) hatch in the
ovisac of the mother and are born live. However, when the body of water where adult Artemia are living begins
to dry up and salinities rise, embryos are encased in a hard capsule, or cyst, so that they are protected and can
hatch later when conditions are better. The cyst is 200 to 300 micrometers in diameter, depending upon the strain.
Its external layer is a hard, dark brown shell (Fig 2). Dry conditions cause the encysted embryo to enter a
dormant state, which allows it to withstand complete drying, temperatures over 100 oC or near absolute zero,
high energy radiation, and a variety of organic solvents. The dehydrated cyst can be stored for months or years
without loss of hatchability.

Figure 2. Artemia Cysts.
Only water and oxygen are required to initiate the normal development of the Artemia embryo, but it
does help the hatch rate to maintain the temperature above 25 oC and place a light near the eggs. The durable,
easily hatched cyst makes Artemia a convenient, constantly accessible source of live feed for the finfish hatchery
operator. Artemia cysts are best stored in a tightly sealed container in a cool, dry environment and, if possible,
vacuum packed. Within 15 to 20 hours after being placed in seawater at 28 o the shell breaks and the prenauplius
in E-1 stage appears (Fig. 3). For the first few hours, the embryo hangs beneath the cyst shell in what is called
the umbrella stage. The newly hatched Artemia relies on its yolk sac for nutrients because its mouth and anus are
not fully developed. The pre-nauplius E-2 stage is then released as a free-swimming nauplius called an Instar 1
nauplius. In this stage it is brownish orange because of its yolk reserves. It uses specially modified antennae for
locomotion and later for food filtering. Approximately 12 hours after hatch it molts into the second larval stage
(Instar II) and starts filter feeding on microalgae, bacteria and detritus. The Artemia nauplius can live on yolk
and stored re-serves for up to 5 days or through the Instar V stage (Fig. 3), but its caloric and protein content
diminish during this time (Briksi et.al., 2008).

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�Figure 3. Steps in Life Cycle of Artemia
1: Cysts, 2: Breaking stage, 3: Umbrella stage: emerging embryo, 4: Instar I(E-1) newly hatched nauplii (with yolk), 5: Instar
II(E-2), 6: Differeatiation (molting) stage, Instar III-IV, 7: Instar VI-VIII, 8: Instar IX-X, Sub-adult stage, 9: Adult stage.

As a food source for the larvae, it is imperative that Artemia is of high quality, as nutritionally complete
as possible, and maintained in this state until consumed by the larvae. There are four distinct stages involved in
Artemia culture. These stages are: (1) decapsulation, (2) hatching, (3) storage, (4) enrichment, (5) harvestin and
usage. Artemia also represent a potential vector for disease introduction into the larviculture production system.
As such, all Artemia production and storage procedures must be conducted utilizing hygienic production
protocols and proper hatchery sanitation procedures. This document provides the background, rationale, and
detailed production protocols for all stages of high-quality Artemia culture to developments on the aplication of
Artemia in the ornamental fish culture.
2.1. Decapsulation of Artemia Cysts
Artemia represent one of the few live feeds that can be cultured in sufficient numbers and are of
appropriate size for larva to transition to between daphnia, blood worms and weaning diets. During a portion of
their life cycle, Artemia hibernate as a desiccated cyst that is capable of withstanding extreme environmental
conditions for long periods of time. Cysts are easily shipped and are thus the form purchased by aquarists.
However, Artemia cysts can cause problems during larviculture because: 1. The shell of the cyst is indigestible
and may cause intestinal blockage when ingested by larva, 2. Cysts are a potential vector for pathogen
introduction to the culture system, 3. Artemia consume high levels of endogenous energy reserves when hatching
through the cyst shell, 4. Cysts must be physically separated from the live Artemia after hatching. Decapsulation
of Artemia cyst is a process whereby the external shell or chorion is chemically removed from the cyst. This
process addresses the concerns noted above and has become standard practice by fish hatcheries looking to
produce high quality Artemia.
The fry of all the five common ornamental fish species tested (guppy Poecilia reticulata, molly
Poecilia sphenops, platy Xiphophorus maculatus, swordtail Xiphophorus helleri and neon tetra Hyphessobrycon
herbertaxelrodi) could readily feed on the decapsulated cysts, and their performances in terms of stress
resistance, growth and survival are comparable to or better than those fed on Artemia nauplii or Moina. A culture
system for production of on-grown Artemia has also been developed specifically for the use in freshwater
ornamental fish farms (Lim et al. 2003).
2.1.1. Artemia Decapsulation Procedure and Decapsulation Requirements
Artemia cysts: 1 kilogram (kg)
Decapsulation vessel: 20 liters (L)
Chlorine bleach (NaOCl; 5.5%): 8 L at 2-10 degrees Celsius (°C)
Sodium hydroxide (NaOH; 40%): 4 L at 2-10°C
Sodium thiosulfate (Na2S2O3 ): 100 g

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�Harvest bag: 100 micrometer (µm)
2.1.2. Hydration
The first step in the decapsulation procedure is Artemia cyst hydration. Hydration of cysts allows for
separation of the nauplii from the chorion, facilitating the decapsulation process. For this step, Artemia cysts are
placed in either fresh or saltwater at room temperature for approximately one hour, using a concentration of 1 g
of cysts per 15 milliliters (ml) of water. It is important during this step to maintain sufficient mixing via aeration
to keep cysts well suspended. After one hour of hydration, the water and hydrated cysts should be drained
through a 100 µm harvest bag; the concentrated cysts are then placed back into the empty decapsulation vessel.
2.1.3. Decapsulation
For decapsulation, pour the chilled sodium hydroxide solution into the decapsulation vessel with
hydrated cysts, again making sure there is adequate aeration within the vessel to keep cysts suspended. The
chilled bleach should then be added to the cysts to initiate the decapsulation process. Because the chemical
reaction during decapsulation is exothermic, it is helpful to begin with chemical solutions chilled to a
temperature of 2°C to 10°C. These starting temperatures will prevent the temperature of the chemical solution
from exceeding 35°C, which may damage the cysts. As decapsulation progresses, the chorion is chemically
removed, resulting in the cysts gradually changing color from brown to grey, then to orange, and finally to bright
orange. This bright orange color indicates that the process is complete. (Cyst buoyancy can also be used as an
endpoint indicator: when approximately 90 % of cysts sink, the process is complete). The process should take
from one to three minutes, but time may differ due to temperature variations. Cysts can easily be damaged by
overexposure to the decapsulation solution, adversely affecting the resulting hatch rate. It is imperative to closely
monitor the process and standardize it for your particular conditions (Fig. 4).
2.1.4. Decapsulated Cysts Harvest
When it is determined that the cysts are adequately decapsulated, add 75 g of sodium thiosulfate to the
decapsulation vessel to neutralize the chlorine, then immediately begin to drain cysts into the 100 µm harvest bag.
During the harvest process (Fig. 4), rinse with ample amounts of water (fresh or salt) while providing ample
aeration via an air stone to keep decapsulated cysts in suspension. When all decapsulated cysts have been
collected, the remaining sodium thiosulfate should be added to the harvest bag. Continue rinsing the bag until
water runs clear and no presence of chlorine can be detected.
2.1.5 Decapsulated Cysts Storage
Decapsulated cysts can be drained of excess water and stored in an airtight container in a refrigerator (+
4 ºC) for up to 5-6 days. For longer-term storage (two weeks or more), cysts must be dehydrated by placing them
in aerated brine (330 g of sodium chloride (NaCl) per liter of water) at the concentration of 1 g of cysts per 20 ml
of brine for 24 hours. They can then be drained and placed into a suitable container, topped with fresh brine, and
placed in a refrigerator (Fig. 5).

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�Figure 4. Harvesting Decapsulated Artemia

Figure 5. Decapsulated Artemia Cysts Hatching Storage

2.2. Hatching of Artemia Cysts and Hatching Requirements
Temperature: 26-30°C
pH: 8.0-9.0
Dissolved oxygen: &gt; 4 mg/L
Light level: ~2000 lux
Salinity: 25-35 parts per thousand (ppt)
Hatching density: ≤ 2 g dry cysts/L
(up to 5 g/L with supplemental O2)
Sodium bicarbonate (NaHCO3): 0.5 g/L
Antifoam (silicone based): 1 ml/100 L
Fill a clean, cone-bottomed hatching tank with warm, filtered seawater or fresh water addet 30-35 g salt.
If warm seawater is not available, allow enough lead time for water to be warmed to 26°C to 30°C in the
hatching tank via submersible heaters. Add 0.5 g of sodium bicarbonate per liter of water in order to maintain the
pH between 8.0 and 9.0 throughout the entire hatching process. The use of antimicrobial products such as
INVE’s Hatch Controller can be used to help minimize growth of pathogenic bacteria in the hatching tank. The
proper stocking density for nondecapsulated cysts is approximately 2-3 g (max. 5 g) per liter. When using
decapsulated cysts, approximately 5 g per liter can be stocked. These numbers can be doubled through the use of
pure oxygen supplementation, which is needed to maintain dissolved oxygen levels greater than 4-5 milligrams
per liter. Attempting to hatch at higher stocking densities can result in physical damage to the nauplii and
reduced quality It is important to maintain sufficient aeration at the bottom of the cone to keep cysts suspended.
When hatching large volumes of cysts, it is advantageous to use a food-grade antifoam product to minimize
excessive foaming in the culture. Hatching times will vary based on strain and age of cysts, temperature and
salinity of water, etc. Thus, it is important to minimize variation between hatches for consistency. Generally,
Artemia require 18 to 24 hours of incubation to hatch. Decapsulated cysts, however, may be ready to harvest
after only 16 hours of incubation. When feeding nauplii directly to fish, timing of the hatch is very important. If
nauplii remain in the hatching tank for too long, they will grow too large and their nutritional quality will
decrease. Determining the endpoint of the hatch should be made through microscopic observation of the relative
numbers of hatched nauplii, prehatched nauplii, and unhatched cysts (Fig 6).

Figure 6. Artemia Hatching Cone
(pure oxygen injection regulators on wall and wire from submersible heater on front edge of tank)

The harvesting procedure varies depending upon whether decapsulated or nondecapsulated cysts were
hatched. Harvesting of Artemia nauplii is done after 5 to 10 minutes interruption of the aeration and remove the
airstone. Wait approximately 5 minutes for the empty casings to float to the surface of the water. Empty cyst
shells float to the surface, while the nauplii concentrate in the lower part of the tank and the unhatched cysts
accumulate underneath the nauplii. Since most nauplii are positively phototactic, their concentration can be
hastened and increased by shading the upper part of the hatching container with a black plastic sheet so that light
reaches the lower part of the container only. Remove the unhatched cysts for the second hatching, after which
the nauplii can be collected. A second collection of nauplii may be done 5 to 10 minutes after the first. Newly
hatched nauplii should then be collected in the harvest bag and rinsed for at least five minutes. If nauplii have

651

�settled properly, only 75 percent of the water column will need to be drained. While harvesting, check on the
relative ratio of nauplii to cysts by transferring a sample to a glass beaker. This will help determine when the
harvesting process is finished or if more time is needed to allow Artemia to settle. Remove the unhatched cysts
for the second hatching, after which the nauplii can be collected. A second collection of nauplii may be done 5 to
10 minutes after the first. The nauplii are now ready to be fed to your fish, transferred to subsequent enrichment,
or placed into cold storage.
2.3. Enrichment of Artemia and Enrichment Requirements
Temperature: 25°C
pH: 8.0-8.5
Dissolved oxygen: &gt; 4 mg/L
Salinity: 20-30 ppt
Density: ≤ 300 nauplii/ml
DC DHA dosage: 0.6 g/L
Enrichment duration: 20-24 hours
Before being fed to larvae, Artemia nauplii are usually fed a specialized diet in order to increase their
size and nutritional profile. While freshly hatched Artemia nauplii are rich in protein and can serve as a bridge
between daphnia, rotifer and enriched Artemia for many species, they are largely void of the beneficial fatty
acids required for proper growth and development of most larvae. For the purpose of the following Artemia
enrichment procedure, the protocol developed for the use of the INVE product, DC DHA SELCO, will be
utilized.
Olivotto et al. (2006) studied on growth and metamorhosis larvae of Sunrise Dottyback, Pseudochromis
flavivertex. Larvae were divided into different experimental groups and fed on different feeding combinations in
order to test the importance of food enrichment on larval survival, growth and metamorphosis timing. A first
group (Group A) was fed on enriched Brachionus plicatilis and enriched Artemia nauplii; a second one (Group
B) on enriched B. plicatilis and not enriched Artemia nauplii and a third one fed on not enriched live preys
(Group C) used as control group. Live prey enrichment was essential for rearing this species. In fact, larvae fed
on not enriched live preys did not past day 7. Highest survival rates (39% juveniles) were observed in Group A
with respect to Group B (11% juveniles). Moreover, evidences of the importance of enrichment on growth and
metamorphosis timing were observed since larvae reared using enriched live preys grew faster and completed
metamorphosis earlier than those fed on not enriched Artemia nauplii. The results presented here provide
additional evidence of the importance of live prey enrichment in ornamental larval fish rearing.
2.3.1. Artemia Enrichment Procedure
There are a number of commercially available Artemia enrichment products. Because these products
have different ingredients, nutritional profiles, and enrichment protocols, it is up to hatchery managers to decide
which product is most suitable for their conditions and species. Once an enrichment product is chosen, it is
important that standardized protocols be developed and strictly followed. Slight changes in temperature or
enrichment time, for example, can have significant effects upon the size and nutritional quality of the final
product. Preparation of enriched Artemia requires a two-day lead time: one day is required for hatching of
Artemia (see Artemia hatching protocol) and a second day for the enrichment process. Having a second,
dedicated enrichment tank is necessary to facilitate this process. As with hatching, a cone-bottomed tank is ideal
for enrichment and helps to ensure adequate mixing and complete draining during harvest. Prior to stocking, the
enrichment tank should be filled with a suitable amount of water, and water-quality parameters (salinity,
temperature, and pH) must be adjusted to match the requirements listed above. It is important to begin the
enrichment process with healthy, high-quality nauplii. Nauplii that are damaged or sluggish prior to enrichment
will result in suboptimal nutrient uptake. Care should be taken to remove hatched cysts (nondecapsulated cysts)
or hatching membranes (from decapsulated cysts) as described in the Artemia hatching section. Artemia nauplii
should also be rinsed well prior to stocking into the enrichment tank. This is especially important when using an
additive such as INVE’s Hatch Controller or antifoam during the hatching process, as ingredients in these
products can interfere with enrichment uptake.
During enrichment, vigorous aeration should be applied through the bottom of the enrichment vessel,
and dissolved oxygen levels should be closely monitored throughout the process (Fig. 7). The use of
supplemental oxygen during this stage will likely be necessary to maintain oxygen levels above 4 milligrams per
liter. Temperature must also be maintained at 25°C through the use of submersible heaters or ice packs, as
dictated by ambient conditions (Delbos, 2009).

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�Figure 7. Multiple Artemia Enrichment Cones (heavy aeration)

2.4. Harvest and Cold Storage
At the end of the enrichment process, the entire volume of water should be drained into a 100-125 µm
harvest bag with sufficient aeration to keep enriched Artemia in suspension. Oxygen levels should be closely
monitored in the harvest bag. The bag containing the Artemia should be rinsed well for five minutes or until the
water runs clear. Thereafter, Artemia should be transferred into a container containing clean water of a known
volume, aerated vigorously, and enumerated as discussed above. If Artemia will not be fed to larvae immediately,
it should be placed directly into cold storage, as described below. Artemia not fed to larvae or enriched
immediately needs to be stored under cold conditions. Cold storage of Artemia dramatically decreases its
metabolism, which directly reduces further growth and metabolism of their protein and lipid stores. Artemia
should be transferred to a cooler or suitable container and stored at 2°C to 10°C, with adequate aeration to
prevent settling (Fig. 8). Under these conditions, Artemia can be concentrated as high as 5,000 per milliliter and
stored for up to 24 hours (Delbos, 2009).

Figure 8. Cold-Banked Artemia
(ice jugs for temperature control and air line for aeration to keep Artemia suspended)

3. Conclusions
The ornamental fish producer would have no problem to assign such a small area for setting up the
culture system in their aquarium or farms. While the use of a batch culture system instead of a flow-through
system would cut down the volume of seawater required for Artemia culture, the use of artificial seawater would
enable farms that have no access to seawater to operate the system. To cut down the cost of salts required for
653

�preparation of artificial seawater, the present system, for the first time in commercial Artemia production, used
diluted artificial seawater (salinity 30-40 ppt) instead of full strength seawater for the culture. Change of water
was not necessary during the 14-16 day culture period. These characteristics made the system suitable for
operation in freshwater ornamental fish farms, and would allow existing ornamental fish farmers to integrate the
Artemia production system in their farm operation. The present system did not use expensive mechanical and
biological water treatment equipment such as bio-filter, mechanical filter, plate separator, sensors etc. and hence
the cost of setting up the system was € 90,000-100,000 only. Bioencapsulation to enhance the nutritional quality
of on-grown Artemia was conducted only when the Artemia failed to meet the fish requirement. The same
applied to all other live food organisms such as rotifers and Artemia nauplii which might also require
bioencapsulation due to their nutritional deficiency (Leger and Sorgeloos 1992; Sorgeloos and Leger 1992;
Sorgeloos et al. 1995, Sorgeloos et. al., 2001). It was performed by fish farmers just before feeding the Artemia
to fish, and not by producer of the organism. Hence the cost of bioencapsulation was not included in the
production cost of the Artemia. Nevertheless, the cost of the enrichment media (€ 80-90/kg) used in
bioencapsulation was estimated to be € 3-5/kg of on-grown Artemia (in 50 liter of water at 0.6 g/L.). The present
Artemia culture system is a cheap alternative to the more sophisticated intensive system used in sectoral
aplications. Compared to the complex automated system, the present system is cost effective, simple and easy to
set up and operate. As the system occupies only a small land area and uses diluted artificial seawater for culture,
the freshwater ornamental farmers will have no problem to integrate Artemia production using the culture system
into their farm operation to increase farm profitability. By varying the time of harvesting, farmers may harvest
any specific size of on-grown Artemia of up to 5 mm from the culture system to suit the age and size of their fish.
The use of the right size of on-grown Artemia for feeding would ensure a better energy balance in food uptake
and assimilation, thereby improving the performance of the fish. These characteristics, coupled with the use of
bioencapsulation technique to enhance the quality of the on-grown Artemia, would make the organism an ideal
nursery diet for freshwater ornamental fish. The availability of on-grown Artemia and the application of
bioencapsulation techniques using the organism are likely to have a positive impact to the ornamental fish
industry.
The food value of a live food organism for a particular fish species was primarily determined by its size
and form. While a small food organism was desirable for fish larvae in term of ingestibility, the use of larger
organisms was more beneficial as long as the size of the food organism did not interfere with the ingestion
mechanism of the predator (Merchie 1996). Fish would take a long time to attain satiation if fed with smaller live
food organism, and this would result in poor growth due to inefficient feeding and waste of energy. The ongrown Artemia in the culture system grew from 0.45 mm at inoculation to an average length of about 5 mm in 12
days. This size range was considered suitable for all sizes of freshwater ornamental fish species of up to 10 cm
total length. By varying the harvesting time during the 12-day cycle, it was possible to obtain Artemia of any
specific size within the size range for feeding, which would ensure a better energy balance in food uptake and
assimilation. The nutritional quality of on-grown Artemia was comparable or superior to the common food
organisms being used by the freshwater ornamental fish industry, such as Artemia nauplii, Moina and
bloodworms. The on-grown Artemia was rich in protein (67 %) and low in crude fat (4 %). It was reported to
have superior nutritional digestibility and a thin exoskeleton rich in essential amino acids (Leger et al. 1989).
The latter was consistent with our amino acids analyses, which showed that the essential amino acids in the ongrown Artemia were comparable to Moina and richer than Artemia nauplii and bloodworms. An important
dietary characteristic of live food organism was its composition of essential fatty acids. Watanabe (1987)
reviewed the essential fatty acid requirement of freshwater and marine fish and concluded that freshwater species
required mainly LLA (linolenic) or LNA (linolenic acid) or both. Although the on-grown Artemia obtained from
the present study was deficient in LNA, its LLA was the highest among all the four diets tested. The DHA
(docosahexaenoic acid) and EPA (eicosapentaenoic acid), which were widely considered as essential for marine
organisms (Dhont and Lavens, 1996), were also highest in on-grown Artemia. Due to lack of published data, it
was not known whether the levels of LLA, LNA, EPA and DHA in food organisms would be important to
freshwater ornamental fish. Recent study on the fatty acid profiles of common feed items used by the industry
for maturation such as beef heart and tubifex worms found unusually high ADA (arachidonic acid) levels (Ako
et al. 1999). Availability of the on-grown Artemia would offer our farmers and exporters the possibility to apply
the bioencapsulation technique to improve their fish performance and quality. In addition, the effective
bioencapsulation characteristics of on-grown Artemia also make the organism a useful tool for larval nutrition
study on freshwater ornamental fish. The present Artemia culture system is a cheap alternative to the more
sophisticated superintensive system. By varying the time of harvesting, aquarists may harvest any specific size of
on-grown Artemia of up to 5 mm from the culture system to suit the age and size of their fish. The use of the
right size of on-grown Artemia for feeding would ensure a better energy balance in food uptake and assimilation,
thereby improving the performance of the fish. These characteristics, coupled with the use of bioencapsulation
technique to enhance the quality of the on-grown Artemia, would make the organism an ideal nursery diet for
freshwater ornamental fish. The availability of on-grown Artemia and the application of bioencapsulation
654

�techniques using the organism are likely to have a positive impact to the ornamental fish industry. Finally,
demonstrated that the commercial production of on-grown Artemia using the present culture system was highly
viable for freshwater ornamental fish applications.

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Briksi, E., Stappen VG., Bossier, P., Sorgeloos, S. (2008). Laboratory production of early hatching Artemia sp. cysts by
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in different media (Anostraca). Crustaceana 49: 318–320.
Leger P., Bengston D.A., Sorgeloos P. (1989). Analytical variation in the determination of the fatty acid composition of
Standard preparations of the brine shrimp Artemia. Aquat. Toxicol. Hazard Assess 12: 413–423.
Leger, P., Sorgeloos, P. (1992). Optimized feeding regimes in shrimp hatcheries. In: Marine Shrimp Culture: Principles and
Practices (Eds.) Fast A. W. &amp; J. Lester, (pp. 225-244). Elsevier Science Publishers.
Lim, L.C., Soh, A., Dhert, P., Sorgeloos, P. (2001). Production and application of on-grown Artemia in freshwater
ornamental fish farm, Aquaculture Economics &amp; Management, 5: 3, 211-228.
Lim, L.C., Cho, Y.L., Dhert, P., Wong, C.C., Neils, H., Sorgeloos, P. (2002). Use of decapsulated Artemia cysts in
Ornamental fish Culture. Aquaculture Research, 33: 575-589.
Lim, L.C., Dhert, P., Sorgeloos, P. (2003). Recent developments in the application of live feeds in the freshwater ornamental
fish culture. Aquaculture, 227: 319-331.
Merchie, G. (1996). Use of nauplii and meta-nauplii. In: Manual on the Production and Use of Live Food for Aquaculture
(Eds.) P. Lavens &amp; P. Sorgeloos, (pp. 137-163). FAO Fisheries Technical Paper 361, FAO, Rome.
Olivotto, I., Rollo, A., Sulpizio, R., Avella, M., Tosti, L., Carnevali, O. (2006). Breeding and rearing the Sunrise Dottyback
Pseudochromis flavivertex: the importance of live prey enrichment during larval development. Aquaculture, 255: 480-487.
Sorgeloos, P., Leger, P. (1992). Improved larviculture outputs of marine fish, shrimp and prawn. Journal of the World
Aquaculture Society, 23(4), 251-164.
Sorgeloos, P., Dehasque, M., Dhert, P., Lavens, P. (1995). Review of some aspects of marine fish larviculture. International
Council for the Exploration of the Sea Marine Scientific Symposium, 201, 138-142.
Sorgeloos, P., Dhert, P., Candreva, P. (2001). Use of the brine shrimp, Artemia spp. in marine fish larviculture. Aquaculture,
200: 147–159.
Stottrup, G.J., McEvoy, L.A. 2003. Live Feeds in Marine Aquaculture. Blackwell Science Ltd., USA. 318p.
Tamaru, C.S., Ako, H. Paguirigan, R., Pang, L. (2001). Enrichment of Artemia for use in Freshwater Ornamental Fish
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�Watanabe, T. (1987). The use of Artemia in fish and crustacean farming in Japan. In: Artemia Research and its Applications.
Vol. 3, Ecology, Culturing, Use in Aquaculture (Eds.) P. Sorgeloos, A. Bengtson, W. Decleir &amp; E. Jaspers, (pp. 372-393).
Universa Press, Wetteren, Belgium.

656

�</text>
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                <text>Recent Developments On The Aplication Of Artemia  In The Ornamental Fish Culture</text>
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Turkmen, Gurel</text>
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                <text>Production of animals for the aquarium hobbyist trade is a rapidly growing sector  of the aquacultural industry, and it will continue to become more important as restrictions are  placed on collecting animals for the wild. Improved techniques for marine food-fish  larviculture since the early 1980’s have greatly enhanced the growth and survival of  freshwater ornamental fish larvae largely through improved technology regarding live food  culture and larval rearing practices. Research developments in larviculture and early rearing  technology have allowed 90% of currently marketed freshwater ornamental fish to be cultured.  However, for marine ornamentals, the reverse is true as only a handful of species is produced  via aquaculture technology. A major task in devising a protocol for the artificial propagation  of a fish species is the development of a feeding regimen for the larvae. Live feeds are a  convenient and often essential food source for the larvae of some cultured species, especially  those without a fully developed digestive system. In such cases, live food organisms provide  digestive enzymes that breakdown the food ingested by larvae and can be described as  naturally encapsulated bags of nutrients. Two major concerns among aquaculturists are  providing organisms appropriate to the size of the larvae at the first feeding stage and then  supplying the large numbers of feed organisms necessary to maintain the larvae. Since no  artificial feed formulation is yet available to completely substitute for Artemia, feeding live  prey to young fish larvae still remains essential in commercial hatchery operations. This paper  reports the recent developments in the applications of Artemia nauplii, decapsulated Artemia  cysts and on-grown Artemia in the ornamental fish culture.</text>
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                    <text>Economic Importance and Using Purposes of Gypsophila L. and
Ankyropetalum Fenzl (Caryophyllaceae) of Türkiye
Mustafa Korkmaz
Faculty of Science and Art, Department of Biology,
Erzincan University, Türkiye
korkmazmustafa67@yahoo.com.tr
Hasan Özçelik
Faculty of Science and Art, Department of Biology,
Süleyman Demirel University, Türkiye
ozcelik@fef.sdu.edu.tr
Fevzi Özgökçe
Faculty of Science and Art, Department of Biology,
Erzincan University, Türkiye
fozgokce@gmail.com
Abstract: Gypsophila L. is the third biggest genus of Caryophyllaceae family in
Türkiye. 55 species of the genus have been growing naturally in our country. 33
of them are endemic and total number of the taxa is 55. Ankyropetalum Fenzl is a
small genus with 3 species and 1 of them is endemic. It is agreeable that gene center
of the both genera is Türkiye. In terms of growing habitats there are large areas in
Türkiye. According to importance order East, Central and Southeast Anatolia regions
have the biggest number of taxa growing there. Ankyropetalum genus distributed only
in the Southeast Anatolia and Mediterranean regions and in their intersection areas of
Türkiye.
Both of the genera have known as “çöven, çöğen”, halvah root and largened root
parts or rhizomes are economically very important. Extracts produced from under
parts of the plants known as fire extinguisher, gold polishing, silk and cloth cleaner
and softener and crispness giving to halvah. These extracts have often used for
making liqueur, preparing herbal cheese and making ice cream. Because of giving
flavour, crispness and nice odor they generally preferred in food industry.
With different ratios all of the taxa are boron (B) hyperacumulators. For this reason
they can be used for destroyed agricultural areas. They can be planted to elevated
slopes and hills to control erosion and survive biological diversity. General character
of the family is their importance for horticulture. G. paniculata is very important for
horticulture industry. In the presentation, some information about economic
importance of the plants in the light of our observations and literatures were given.
Key Words: Gypsophila, Ankyropetalum, Economy, Flora of Türkiye

Introductıon
Turkey is known as a gene centre of many economic groups of plants. In Turkey there are 32 genus and
around 500 species of Caryophyllaceae family [1-6]. It is reported that the centres of some regions in which the
species belonging to the Gypsophila genus are pervasive are Turkey, Caucasian, the North Iraq and the North
Iran; that 75 out of 126 Gypsophila species in the world are found in this region and that in Turkey there have
been found 55 Gypsophila species in 10 sections [7-8]. In the world Ankyropetalum genus has 4 species, and
3 of which grow in Turkey. The gene centre of both genus is Turkey.[9].
In general, soaproot is the woody roots of some perennial species of the genera Gypsophila L.,
Saponaria L., and Ankryopetalum Fenzl, belonging to the Caryophyllaceae family. However, Saponaria is not
used as soaproot in Türkiye [10].
Turkish Çöven are commonly obtained from Gypsophila graminifolia Bark. G. arrostii Guss. var.
nebulosa (Boiss. &amp; Heldr.) Bark., G. eriocalyx Boiss., G. bicolor (Freyn &amp; Sint.) Grossh., G. perfoliata L., G.
venusta Fenzl subsp. venusta and Ankyropetalum gypsophiloides Fenzl. [7, 10]. But such species as G.

552

�ruscifolia Boiss. and G. bitlisensis Bark. are less preferred. Since 1800s soaproot has been exported from
Anatolia (Turkey). The leading ones are G. bicolor, G. arrostii ve A. gypsophiloides (Radix Gyspohilae) [11-14].
For nearly 30 years the extraction of Çöven from natural flora has been increasingly continueing in the Eastern
and South-east Anatolia [10].
Ankyropetalum Fenzl is represented by 3 species in Turkey. One of these is endemic and the others are
not widespread. The genus is essentially pervasive in South-west Asia including Turkey. Regarding the
phytogeography the genus grows in the South east of Turkey; that is in Iran-Turan and Mediterranen regions [14,
15]. The species belonging to the Ankyropetalum genus which is pervasive in Turkey are A. arsusianum Ky, A.
reuteri Boiss.&amp; Hausskn. (endemic) and A. gypsophiloides Fenzl. The genus is found in South-east Anatolia and
its neighbour countries in borders [1, 14, 15].
The taxa belonging to the Ankyropetalum and Gypsophila genus and known and used by the public are
generally known by the name “Çöven Otu” . As the Ankyropetalum genus’ members look like perennial
Gypsophila species and as they are distinguished hardly, they are known by the same name and used for the
same purposes. In Europe Gypsophila species are widely known as “Baby’s Breath”. For the word Gypsophila
“Soaproot” or “Soapworth” words are used. In Turkey these plants are called “çöven otu, çevgen, dişi çöven,
tarla çöveni, helva çöveni, şark çöveni” by the local public.
It is reported that the saponins are found in the different parts of the plant in different doses; that they
were first obtained by boiled alcohol from the risoms of Saponaria officinalis and that they are called “saponin”
[11]. It is reported that as the roots of Soaproot are obtained from various Gypsophila species, the saponin
amounts in the roots which are used in the trade differs between (4-)10-20(-25) % [22]. Used as Turkish
Soaproot, in the Gypsophila bicolor (Van Çöveni) the saponin amounts have been found to be 20-25 %, in the
G. arrostii var. nebulosa (Konya, Beyşehir, Isparta Çöveni) 19-22 %, in the G. perfiolata (Niğde Çöveni) 1519 % and in the G. eriocalyx (Çorum- Yozgat Çöveni) 10-14 % [17]. They have found out that in the G.
paniculata the saponins synthesizes only in the roots and then moving through the other parts of the plant and
that in dry material there is around 4 % saponin [20]. They report that in the Soaproot originated in Anatolia the
amount of raw saponin is 10-25 %; and in their searches on the taxa of G. bicolor, G. perfoliata var. anatolica,
G. venusta subsp. venusta, G. eriocalyx and G. arrostii var nebulosa which are pervasive in different parts of
Turkey the amounts of saponin are respectively 19.58 %, 14.44 %, 12.65 %, 12.39 % and 11.58 % [20]; and the
amounts of protein are respectively 8.01 %, 7.80 %, 8.38 %, 8.15 % and 6.92 % [7].
It is stated that in the roots of G. paniculata with the affect of the enzyme of UDPGlucuronosyltransferase the synthesis of saponin has been increased considerably and that in order to define the
activity of this enzyme which has a versatile role in the plants the G. paniculata species would be a good model
plant [17].
The Production of Soaproot Extract
The roots and risoms of Soaproot
↓
Cut in the form of chips
↓
The first boiling (4-5)
↓
The second boiling
↓
The extract of Soaproot

The extract of Soaproot whose production stages and chemical formule have been shown above is
composed of sugar, resin and saponin. Saponins are highly moleculer glycosides which has the characteristic of
solution in water and which are widely seen in some plants especially in Saponaria. It keeps the plant from
germs and fungus and some species of it increases the nutritional value of plant as well as simplifying the
digestion [18].

553

�Where ıs soaproot used?
Its usage in the food industry
It is known that A. gypsophiloides was formerly exported from Siirt and Batman by caravans and that it
is known by such names as “ Helva kökü, Çöven otu, Sabun otu, Helva otu” and that it is especially used in
preparing a local food called “Siirt sweet”. A. reuteri is called “çöven” by the local public around Gölbaşı
(Adıyaman); it is used for animal feding by mixing with straw and it is said that it was formerly used for the
purpose of animal feding [9, 11,12].
In the Eastern Anatolia, the roots of some soaproots are used in preparing a local and origional food
kind “herby cheese”. [8, 13-14]. The use of extract of Soaproot is firstly an obligation in making “tahin helvası
(halvah)”. Otherwise it is impossible to make the halvah crisp. Apart from halvah, it is utilized for the production
of “delight” and “icecream” and also in Thracian region because of its charasteristic of whitening wax and its
power to make crisp soaproot is utilized in the “köpük halvah” which has a white and spumy form. Furthermore,
in some parts of Turkey while making “hellim cheese” after soaproot is cooked it is added to the brine so that the
cheese doesn’t spoil [8, 18, 19].
It is stated that the delight obtained by mixing syrup whitened by soaproot extract with pure delight is
called “sultan delight” and that the maximum saponin amount should be 0.1 % [7]. The main reasons of why
soaproot is most preferred for the halvah production are those; the saponin in the soaproot whitens the sugar wax,
saponin softens the sugar and makes like sponge, and it has a function like emulgator by enabling the sesame oil
to mix with sugar. In halvah production the amounts used are respectively 52-65 % tahini, 35-48 % sugar, 0.5 %
soaproot [17]. In the production of “köpük halvah” soaproot and sugar are boiled in water and added after it
takes the wax form. Soaproot water is used in production of “köpük halvah” which is half-liquid and has a little
hard stiffness. When adding soaproot water there are two elements to be taken into consideration; firstly, its
amount shouldn’t exceed the avarage level and second, when adding soaproot the pot should be covered.
Otherwise the air absorbed leads to overbubbling. When making “köpük halvah” it can be used about 60- 65 %
glycose syrup, 30- 35 % water and 0.01 % soaproot water [18].
Some kinds of Gypsophila arrostii var. nebulosa are economically valuable and in Konya and Beyşehir
it is called “dişi çöven (female soaproot)” because of its multiplying fast. Roots of the other soaproots known as
“erkek çöven (male soaproot)” are not widely used in trade. In their rhizomes there are saponin, resin and sugar.
Because they are widely used in production of “tahini halvah” in Turkey and Near East they are called “helvacı
çöveni (halvah soaproot)”. In some of our cities and in Cybrus in order that the “hellim cheese” which is boiled
and made salted does not spoil, soaproot root is added to its water. In Thracia region a white spumy halvah
known as “köpük halvah” is produced by soaproot [20].
Its Usage in The Chemistry and hygiene Industry
Soaproots are also used in the manufacturing of Saponin which is a valuable chemical substance [8, 21].
Saponins are components which have pervaded in wide districts, which are in the form of heavy molecular
steroidal or triterpenoid glycsides and which have a great biological activity on plants, insects, fungus and
microorganisms. Their lower doses helps the plants have roots; however higher doses decreases root growing [7].
As quoted from Çevrimli (1990); it is expressed that because of the negative impacts of alkil and aril
sulfanat types of detergants on environmental pollution and human health, the usage of saponin present in G.
arrostii as an active surface substance of detergant will be more benefical, the saponin present in the plant will
be easily used as an active surface substance in both extinguishers and soap industry, and that in the plant
rhizomes there has been found around 18 % saponin [7].
Because it has a good characteristic of bubbling, soaproot is being utilized for soap, shampoo materials
or fabric softener in hygiene industry. In the cool water obtained from the soaproot roots boiled, the silky and
delicate fabrics and the other fabrics which are otherwise deteriorated are cleaned. Fabrics or clothes are cleaned
by being dipped into the cool water obtained and are kept waiting for a few hours without spoiling their colors
and brightness. Moreover, in some regions they are used in order to clean the wool obtained from the animals. [8,
18, 19]. The undersoil parts of G. arrostii have been used as a cleaner and a removal of stain since ancient times.
[21].
Its Usage In Medicine
It is reported that in the antraks vaccine which is against Antraks disease seen among animals and which
is produced in Turkey, the saponin amount has been found to be % 0.1- 0.5. It has been found out that saponins

554

�are in the seeds, limbs, leaves, flowers and roots of plants; and that when the plants containing saponin are eaten
by animals, the bitter-flavoured saponins have irritated mucosa cells in throat. Such types as G. paniculata and
G. arrostii are used as a cough and respiration system diseases deterrent besides being used as a myx remover
[7]. Soaproot has some features such as urine remover, exudative and myx remover [18]. As for drug, it has a
function in some drugs compound when they are brewed because of its characteristics such as myx and urine
remover [19].
Its Antimicrobial Effect
It is known that saponin has an antimicrobial effect and it keeps the plants against some insects in soil.
Besides this, it is estimated that it has a role of increasing the plant resistance in some parts of plants. It is
reported that in the soil in which the plant containing saponin grows there has been found to be saponin in
certain amounts and this saponin in the soil has some impacts on some bacteria. It has been found that
Aquaspirillum dispar and Aquaspirillum spp. soil bacteria have been in great numbers in the roots of G.
paniculata [7]. In the search of Gypsophila species’ antiviral impacts it has been expressed that G. arrostii var.
nebulasa, G. bicolor, G. perfoliata and G. eriocalyx species have impacts on V. stomatitis virus, that they have
no impact on Parafainfluenza type-1 virus and that the G. bicolor species has effectiveness against the other
viruses (Poliovirüs tip-1, Herpes simplex tip-1 ve tip-2, Vesicular stomatitis ve Influenza A ) except
2

Parafainfluenza type-1 virus [7, 22].
Its Usage in Horticulture

Gypsophila species are regarded as one of the most important alternatives of product diversification in
the sector of flower cutting. G. paniculata species used as fresh and dry cut flower attract attention as being one
of the most indispensable elements of arrangement and bouquet in domestic market [7, 8].
It has been reported that in Eurasia continent there have been found to be 125 species of Gypsophila,
that the most significant of those to be used as ornamental is G. paniculata; that although the plant is perennial it
has been grown annual and that because it cannot enable blossoming in short time and because it has no genetic
evolution it is more advantagous to multiply it by cutting. It has been expressed that G. paniculata species has a
great importance in the trade of cut flower; that although with the reparation studies the desired plants have been
obtained, from these plants whose seeds are cultivated the desired plants will not be able to obtained and that
they may have genetic evolution so the plants should be grown with vegetative organs. They have found that in
the G. paniculata species which is used in horticulture their harvest should be done when their petals have
exceeded 50 % blossoming, the vase lifespan in plants has reached about 55 days with the blossoms of buds in
vase and that during 82 days the flower harvest can be done on plants. In Gypsophila paniculata species which
is used in horticulture in the flower buds, the flowers should be harvested when they blossomed 30 % and so the
vase lifespan increases. They have informed that because in coastal regions of Mediterranen the floral
deportation obtained in unit area for the production of G. paniculata will be more it can be advised to cultivate
around coastal parts of The Mediterranen Region [7].
Its Usage in Mining
As a result of the studies on natural-growing 4 Gypsophila species in the district of boron (B) mine in
Eskişehir Kırka; it has been observed that G. sphaerocephala Fenzl ex Tchihat. var. sphaerocephala and G.
perfoliata have been the first ones which have a characteristics of a potential boron hyperaccumulator. These
species grow successfully in concentrations as high total soil boron (8900 mg/kg-1) and suitable soil boron (277
mg/kg-1). As a result of the analysis conducted, it has been found out that in the upper soil parts of the G.
Sphaerocephala it has contained B in extremely high concentrations (in seeds; 2093 ± 199 SD mg / kg-1; in
leaves; 3345 ± 341 SD mg / kg-1), but in roots it has contained far less concentrations of B (51 ± 11 SD mg kg1). In the respect of Boron amount this has been followed by G. perfoliata. It has been stated that by growing G.
sphaerocephala in the soils which have some signs of high B toxid, vegetative mining can be conducted by
hyperaccumulation and the soils containing boron in toxic amounts can be refined by vegetative ways [23]. In
this way the agricultural fields in which fertilizers have long been used can be prevented to become barren and
during reparation process it will be possible to evaluate the agricultural fields which have become dormant.
Its Other Usages
Apart from these, the cool water obtained from the boiling of soaproot roots is used in the process of
whitening gold and treasuers. Furthermore this solution is used as spray in the structure of film emilsion and

555

�extinguishers. It is known that the rhizomes of perennial soaproot sold to Israel from Isparta are used in the
production of extinguishers [7, 8, 21].
Pıckıng, dryıng and storıng the plants
Because the subsoil parts of the plants are generally utilized, from just after the precipitation season to
the time of plant’s fruit; that is between May- July the plants are picked. The local public utilizes its root when
its leaves are on land area or when they are in the time of blossoming; they can distinguish Gypsophila species
between others and they can extract its rhizomes by means of such tools as anchor. Because the subsoil parts of
the plant are also picked, with an unconscious picking they are endangered. The roots which are picked are
cleaned and after washing them, they are dried under sun. In order to dry well and in order to enable some fresh
air during this process the rhizomes shouln’t be laid down thick. The subsoil organs are brought in bundles and
are stored in suitable, dry and moisture free places [19].
It is reported by Anonim (2006) that in the roots of soaproot plant which can be grown in barren and
hillside areas there have been foud to be some dryings because of extreme damp; that it is not suitable to harvest
them before four years old; that their trade situation should be considered before harvesting and if necessary the
product should be waited in the field; that the roots extracted by fork or tractor plough have dried in 2-3 months
and from 2.5 kg raw root about 1 kg dry root has been obtained; and that in one decare of field totally 4000-5000
kg dry roots are extracted [7].
The trade of soaproot and ıts standart
There is not a general accepted standart for the roots of soaproot but they can be classified in three
different qualities in terms of commercial purposes according to where they grow: those growing in Van-Isparta
are of the 1. quality, those obtained from Niğde are of 2. quality and those obtained from Yozgat-Çorum are of 3.
quality [19].
In the usage of soaproot in industry, the hemolysis and bubble indexes of them; as for in food industry
the bubble indexes are of importance. So, in the quality evaluation these rates should be taken into consideration.
Both the bubble and hemolysis indexes of Van (G. bicolor) and Isparta-Beyşehir (G. arrostii) are high. Also
their raw saponozite rates are more than the others.

Species

Hemolysis index

Bubble index

% Raw saponozit

G. bicolor
G. arrostii var. nebulosa
G. perfoliata var. anatolica
G. eriocalyx

6.667- 6.925
5.295- 6.667
9.778-10.000
3.385- 3.659

9.000-10.000
9.600-10.034
4.650 - 5.000
1.800- 2.000

20-25
19-22
15-19
10-14

Table 1. The Analysis of Turkish soaproot [22]
Isparta-Beyşehir Gypsophila (G. arrostii) has decreased extremely on the market. There is still Van
Soaproot (G. bicolar) on market and is sold as being the first quality. The oldest commercial soaproot is this
species, so its population has damaged greatly. But this species has been produced in fields (Atabey Plain) by
some farmers. Furthermore, Isparta General Directorate of Forestry cultivated about 15-20 kg seeds 3 years ago
in order to be a financial support in the future and to enable the continuation of the species generation for
Sütçüler and Aksu villagers. The hemolysis index of Niğde soaproot (G. perfoliata var. anatolica) is high but its
bubble index and raw saponozit percentage is low. Despite its features similar to 1. quality, it should be regarded
as second quality. Çorum-Yozgat G soaproot (G. eriocalyx) is one type of soaproot having the lowest rates. In
these respects it should be regarded as the third quality [22].
They are exported to many countries includin Germany, Egypt, Greece at the outset [19]. Between
1989-1996 the avarege annual export of soaproot root was 140 tonnes. In 1997 it decreased to 93.3 tonnes.
Today, every year the avarege export of soaproot root from Turkey is about 90 tonnes. In 2004 80000 dollars
have been earned from the soaproot exportation for 85 ton. According to the statistics of 2005 92 tonnes of

556

�soaproot roots were exported from Turkey by taking 66 000 dollars in return. In 2006 despite 153 tonnes
exportation the income was 61 000 dollars in an unparallel way [7, 24].

Discussion and advices
Six of species growing in Turkey has a high economic value. Besides picking plants in an
uncontrollable way from nature, industrialisation and urbanisation, extension of agricultural fields and extreme
grazing, tourism, the reparation of barren fields, agricultural struggle and pollution, unconscious forestation and
fires are leading factors that threaten the plants in our country [7].
Because many natural plants used in medicine, exported and and used traditionally are constantly being
picked from nature, are exported and used in domestic market, they are increasingly disappearing [7].
Soaproot plants have been utilized in medicine, food, hygiene, as ornamentals in parks and gardens, in
chemistry industry in order to produce saponin. It has the ability to extinguish the fires, whiten gold, clean silky
and delicate fabrics. It also enables cleaning the contaminated soil by removing the boron which is in great
amount in our country. They are clened by cultivating G. sphaerocephala and Gypsophila species. Also it is
possible to make vegetative mining by boron hypercumulation to the upper surface of the plant on soil.
Because they are utilized in many different areas, agriculturalists, food engineers, chemists, pharmacists,
landscapists, textile workers and jewellers are all interested in them [7].
In Turkey the general name of Gypsophila, Ankyropetalum ve Saponaria species are “ çöven ”. But
some researchers name those whose subsoil parts are economically valuable as “çöven”. Regarding this soaproot
is the name of a raw material and is an extract obtained from a plant [9].
Turkish soaproot is widely obtained from 6 Gypsophila (G. graminifolia, G. bicolor, G. arrostii var.
nebulosa, G. eriocalyx, G. perfoliata var. anatolica ve G. venusta ) and 1 Ankyropetalum (A. gypsophiloides
species. The gene centre of both species is Turkey [9].
Gypsophila species yielding soaproot, their locations and some properties are as below [25].
G. bicolor (Turkish names: Van çöveni, Tarla çöveni): This species is distributed around Van, Bitlis
and Artvin provinces. The rhizomes are hard and difficult to break. Saponin content is 20 %-25 %. This value is
higher than that in other soaproot yielding plants. This is the most preferred soaproot, also known as the soaproot
of the highest quality.
G. arrostii var. nebulosa (Turkish name: Beyşehir çöveni, Konya çöveni): Saponin content is 19%-22%.
This is also considered to be of good quality.; This species also has a narrow distribution. Halvah makers in
Konya especially use this soaproot.
G. eriocalyx (Turkish name: Çorum-Yozgat çöveni): Grows around Ankara, Çankırı, Çorum, Eskişehir,
Kayseri, Sivas and Yozgat provinces in steppe habitats with gypsum. This is an endemic species.
G. perfoliata (Turkish name: Niğde çöveni): Grows around Ankara, Kayseri, Sivas, Erzincan, Konya,
Niğde, and Denizli provinces. It is considered to be of 3rd quality.
G. venusta subsp. venusta (Turkish name: Konya çöveni): Grows around Ankara, Çankırı, Konya,
Gaziantep, Urfa, Sivas, Malatya, and Erzurum provinces in stepe habitats or arable fields, and yield soaproot.
G. graminifolia (Turkish name: Başkale çöveni, Dağ çöveni): This local endemic species also grows in
Van province, around Başkale.
Three species of Ankyropetalum genus grow naturally in Turkey; all of them are endemic. However,
only A. gypsophiloides rhizomes are known, with the name “helva (halvah) root” around Siirt province and used
as soaproot. A. gypsophilloides (Turkish name: Siirt çöveni, Helvacı çöveni, Helva kökü): This species grows
around Şanlıurfa, Mardin, Gaziantep, Batman, and Siirt provinces. Its roots are collected in Siirt and used by
local halvah producers. [12].
Because the roots of these plants are generally used, the harvest time is in March-June months and thus
because the plants don’t produce seeds they don’t enable seeds for the latter year. These plants which are
constantly taken from nature both exported and used in domestic consumption and whose economic value is
extremely high, are increasingly disappearing and are on the verge of extinction. This problem becomes more
important especially when the plants are endemic. Except for the G. paniculata which is used for cut flower and
cultivated, Gypsophila species which are used for exportation and domestic consumption and some of which are
endemic are taken directly from natue. This brings the danger of extinction with itself.
Although economically important, these plants are a source of biological richness in Turkiye. Most of
them are endemic species having narrow distributions. Since they are not cultivated but collected directly from
nature, populations of these plants in nature deteriorate, their generations diminish or become extinct, and the
balance of nature is disturbed. Since only roots and not the aerial parts are collected destruction is an even more
important problem. According to some trading companies cheaper soaproot having better quality is being
imported from Afghanistan, and re-exported after the extraction of their juices. This soaproot juice (extract) is

557

�sold to halvah producers in Türkiye. So there is no need to collect soaproot in Turkey anymore. Soaproot has
been collected for years due to the high unemployment rate in the region, and the demand. In order to preserve
these species and also have regular exports, feasibility studies should be conducted and a determined quantity of
a given quality should be cultivated. Soaproot should not only be collected from nature and its cultivation should
be scheduled. Decrease in collection due to decreasing demand and soaproot imports from Afghanistan are good
news. Soaproot imports may stop some day, but soaproot usage will continue and so we should take necessary
precautions. Soaproot collection in Turkey should be stopped or at least alternation should be applied to
collection areas. Cultivation of soaproot yielding plants, should be studied and encouraged. Standardised extract
of soaproot should be prepared. Soaproot yielding other perennial species and their saponin contents should be
determined and new soaproot resources should be identified, and their cultivation and marketing possibilities
should be studied. Informtions must be given to our puplic to preserve our biological richness [ 25].

Acknowledgement
This study is a part of the project numbered TÜBĐTAK-TBAG 107 T 147, which is supported by TÜBĐTAK, and themed
“Revision of Gypsophila L. and Ankyropetalum Fenzl (Caryophyllaceae) Genera and Determination of Saponin Level”.
We are much obliged to the support provided by this institution.

References
[1] Davis, P.H., (ed). 1965-1988. Flora of Turkey and the East Aegean Islands, Vol.:1-10, Edinburgh Univ. Press.
[2] Williams, F. N., 1989. Revision of The Forms of The Genus Gypsophila L., Jown Bot. London, 27: 321-329.
[3] Chopra, G.L., 1966. Angiosperms (Systematic &amp; Life-Cycle), s: 85.
[4] Lawrence, H.M.G.,1951. Taxonomy of Vascular Plants. Cornell University, Newyork, s: 486–488.
[5] Huber-Morath, A., 1967. Gypsophila L., Ankyropetalum Fenzl in Davis, P.H.(ed.). Flora of Turkey and the East Aegean
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[6] Güner, A., Özhatay, N., Ekim, T., Başer, K.H.C., 2000. Flora of Turkey and the East Aegean Islands, Vol.:11,
Edinburgh University Press. Edinburgh.
[7] Đnan, M., 2006. Çukurova Koşullarında Farklı Kökenli Gypsophila L. Türlerinde Kök Verimleri ve Saponin Đçeriklerinin
Araştırılması. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Tarla Bitkileri A. B. Dalı (Doktora Tezi).
[8] Korkmaz, M., 2007. Türkiye’ de Yetişen Tek Yıllık Gypsophila L. (Caryophyllaceae) Taksonları Üzerinde Biyosistematik
Çalışmalar, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Isparta, 248 s. (Doktora Tezi).
[9] Özçelik, H., Muca, B., 2010. Ankyropetalum fenzl (caryophyllaceae) cinsine ait türlerin türkiye’deki yayılışı ve habitat
özellikleri, nobel dergisi( bibad)
[10] Kılıç, C.S., Koyuncu, M., Güvenç, A., 2008. Soaproot Yielding Plants of East Anatolia and Their Potential in Nature,
Turk. J. Bot. 32(2008) 489- 494.
[11] Baytop, T., 1984. Türkiye’de Bitkiler Đle Tedavi, Đstanbul Üniversitesi Yayınları, s: 213-214, Đstanbul.
[12] Öztürk, M., Özçelik, H., 1991. Doğu Anadolu’nun Faydalı Bitkileri (Useful Plants of East Anatolia) SĐSKAV Yayınları,
Semih Ofset ve Matbaacılık, Ankara.
[13] Özçelik, H., Özgökçe, F., 1999. Gypsophila bitlisensis Bark. ve Gypsophila elegans M.Bieb. Üzerinde Morfolojik,
Taksonomik ve Ekolojik Araştırmalar, 1st International Symposium on Protection of Natural Environment and Ehrami
Karaçam, 23-25th September 1999, Kütahya / Türkiye, 295- 313.
[14] Özçelik, H., Özgökçe, F., 1995. Taxonomic Contributions to Genus Gypsophila L.(Caryophyllaceae) from East Anatolia
(Turkey), IV th Plant Life in Soutwest and Central Asia (Ed. M.Öztürk, Ö. Seçmen and G.Görk), Ege Univ. Pres, Đzmir,
Türkiye, 195- 209.
[15] Afifi, F.U., Abu-Irmaileh, B., 2000. Herbal Medicine In Jordan With Special Emphasis on Less Commonly Used
Medicinal Herbs, Journal of Ethnopharmacology, 72, 101–110.

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�[16] Boissier, E., 1867. Flora Orientalis, Vol: 1, s: 532-534, Genevae.
[17] H´erold, M-C., &amp; Henry M., 2001. UDP-Glucuronosyltransferase activity is correlated to saponin roduction in
Gypsophila paniculata root in vitro cultures, Biotechnology Letters, 23: 335–337, Netherlands.
[18] Anonim, 2010. www.hammaddeler.com.tr
[19] Orman Genel Müdürlüğü, 1991. Ülkemizde Bazı Önemli Orman Tali Ürünlerinin Teşhis ve Tanıtım Kılavuzu, Orman
Bakanlığı, Ankara.
[20] Battal, H., 2002. A Research on the production of a soapwort extract, Ankara University Graduate School of Natural and
Applied Sciences. Department of Food Engineering. Master Thesis. Ankara, Turkey, pp: 44 .
[21] Anonymus, 2010. www.bibilgi.com/ÇÖVEN-(ÇÖĞEN)-OTU-(Gypsophila arrostii)
[22] Sezik, E., 1982. The Origin and the Quality of the Turkish Soaproots. J. Fac Pharm Ankara 12: 41-54.
[23] Babaoğlu, M., Gezgin, S., Topal, A., Sade, B., Dural, H., 2004. Gypsophila sphaerocephala Fenzl ex Tchihat.: A Boron
Hyperaccumulator Plant Species That May Phytoremediate Soils with Toxic B Levels, Turk J Bot, 28 (3): 273-278.
[24] Anonymus, 2009, Dış Ticaret Đstatistikleri, 2009.
[25] Koyuncu, M., Kılıç, C.S., Güvenç, A., 2008. Soaproot Yielding Plants of East Anatolia and Their Potential in

559

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                <text>Economic Importance and Using Purposes of Gypsophila L. and  Ankyropetalum Fenzl (Caryophyllaceae) of Türkiye</text>
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Özçelik, Hasan
Özgökçe, Fevzi</text>
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                <text>Gypsophila L. is the third biggest genus of Caryophyllaceae family in  Türkiye. 55 species of the genus have been growing naturally in our country. 33  of them are endemic and total number of the taxa is 55. Ankyropetalum Fenzl is a  small genus with 3 species and 1 of them is endemic. It is agreeable that gene center  of the both genera is Türkiye. In terms of growing habitats there are large areas in  Türkiye. According to importance order East, Central and Southeast Anatolia regions  have the biggest number of taxa growing there. Ankyropetalum genus distributed only  in the Southeast Anatolia and Mediterranean regions and in their intersection areas of  Türkiye.  Both of the genera have known as “çöven, çöğen”, halvah root and largened root  parts or rhizomes are economically very important. Extracts produced from under  parts of the plants known as fire extinguisher, gold polishing, silk and cloth cleaner  and softener and crispness giving to halvah. These extracts have often used for  making liqueur, preparing herbal cheese and making ice cream. Because of giving  flavour, crispness and nice odor they generally preferred in food industry.  With different ratios all of the taxa are boron (B) hyperacumulators. For this reason  they can be used for destroyed agricultural areas. They can be planted to elevated  slopes and hills to control erosion and survive biological diversity. General character  of the family is their importance for horticulture. G. paniculata is very important for  horticulture industry. In the presentation, some information about economic  importance of the plants in the light of our observations and literatures were given.</text>
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                    <text>Accumulation of Heavy Metals in Some Plants Grown on Serpentine Soils
of Mersin, Turkey
Nurcan Koleli
Mersin University, Faculty of Engineering, Department of Environmental Engineering, 33342 Mersin, Turkey
nkoleli@mersin.edu.tr
Aydeniz Demir
Mersin University, Faculty of Engineering, Department of Environmental Engineering, 33342 Mersin, Turkey
aydenizdemir@mersin.edu.tr
Muzeyyen Eke
Mersin University, Faculty of Engineering, Department of Environmental Engineering, 33342 Mersin, Turkey
muzeyyeneke@hotmail.com
Ozlem Cakmak
Cukurova University, Faculty of Agriculture, Department of Soil Science, 01330 Adana, Turkey
ocakmak@cu.edu.tr

Abstract: The purpose of this study was to determine hyperaccumulator species growing in
Findikpinari-Mersin. The soils derived from ultrafamic rocks lead to unusual and sparse
associations of flora that are tolerant to extreme environmental conditions such as high heavy
metal contents. As the geological structure, Mersin-Findikpinari has rocks containing
ultramafic and serpentine, but this site is one of the less studied areas. The 26 specimens of
total 755 plants identified systematically from Mersin-Findikpınari in between in 1997-2002
were randomly selected and studied whether hyperaccumulator or not. Twenty six plants
collected (members of 26 genera and 8 families) from different sampling locations were
analyzed for their total As, Cd, Co, Cr, Cu, Mn, Ni, Pb, Se and Zn concentrations using an
ICP-MS. A certified reference material (SRM 1573A, SRM 1547) was also analyzed to check
the accuracy of the used extraction technique. In the present study, Mn content (548 mg kg-1)
of Anthemis aciphylla Boiss. (Asteraceae) was higher than the critical Mn value (300-500 mg
kg-1) and Ni content (115 mg kg-1) Crocus graveolens Boiss&amp;Reute (Iridiceae) was higher
than the critical Ni value (10-100 mg kg-1) but unfortunately none of the plants studied was
hyperaccumulator.
Keywords: hyperaccumulator, Findikpinari-Mersin, serpentine, heavy metal

Introduction
Heavy metal contamination in soil is a global environmental and health safety issue in the world.
Remediation of contaminated soils is essential for sustainable soil use. Conventional remediation technologies
for soils contaminated with heavy metal cations are generally termed as ‘pump and treat’ and ‘dig and dump’
techniques (Chin, 2007). They can be divided into either in situ or ex situ remediation. The conventional
technologies used for in situ and ex situ remediation are typically expensive and destructive (Prasad and Freitas,
1999). The environmental impact of such technologies can be very high. For example, soil washing methods
may render the soil infertile or spread the contaminant, and excavation methods can produce high waste volumes.
Additionally, these remediation methods are often limited to small areas and depend on accessibility to the
contaminated site (Chin, 2007). The high cost and environmental concerns of conventional remediation
technologies has fuelled the need for alternative remediation method. Phytoremediation is one of alternative
remediation technologies (Chaney et al., 1997; Chin, 2007). Phytoremediation is defined as the use of green
plants to remove pollutants from the environment or render them harmless (Raskin et al., 1997). The five classes
of phytoremediation are outlined below. (i) Rhizofiltration, (ii) Phytostabilisation, (iii) Phytodegradation, (iv)
Phytovolatilisation, (v) Phytoextraction (Chin, 2007). The phytoextraction and rhizofiltration technologies are
the most useful branches for heavy metal removal from soil and water respectively. The goal of phytoextraction
is to reduce heavy metal levels in the soil to acceptable levels within three to ten years (Huang and Cunningham
et al., 1996). In order to achieve this goal, plants must be screened and selected for certain attributes. The ideal
499

�plant for phytoextraction would have: (i) a rapid growth rate, even under harsh conditions, (ii) a high shoot
biomass (20 metric tons dry mass (DM) ha-1 yr-1) (Huang et al., 1997), and (iii) a capacity to accumulate or
tolerate high amounts of metals in shoots; in the case of Pb, 10,000 mg kg-1 (1% DM) (Brooks, 1998). There are
three types of metal-tolerant plants which are classified according to their tolerance and accumulation response
on soils contaminated with heavy metal cations: (i) excluders - restrict metal uptake into roots except at extreme
metal concentrations (ii) indicator plants - metal level accumulated in the shoot is relative to metal levels in soil
and (iii) hyperaccumulators – concentrate metals in shoots, regardless of soil metal concentrations (Greger,
1999; Ghosh and Singh, 2005). Metal hyperaccumulator plants comprise species that accumulate (in mg kg1
)&gt;10000 (Mn or Zn), &gt;1000 (Cu, Co, Cr, Ni, Pb) or &gt;100 (Cd) in their shoots (Baker and Brooks, 1989; Wenzel
and Jokwer, 1999). Initial phytoextraction research began with hyperaccumulator plants, such as Thlapsi
caerulesences and Alyssum bertoloni (Keller et al., 2003). Whilst these plants are useful for studying metal
tolerance and accumulation mechanisms, their slow growth rate and small biomass may limit their application in
phytoremediation (Ebbs and Kochian, 1998). This is because the total amount of metals extracted (a measure of
phytoremediation potential) is the product of biomass and tissue concentration (Kayser et al., 2000). Of the over
450 plant species which have been identified as hyperaccumulators, about 75% of their have been Ni
hyperaccumulators (Clemens, 2001). These hyperaccumulator plants have attracted the interest of plant and soil
scientist because of their role in the development of phytoremediation technologies for the treatment of heavy
metal contaminated soils, sediments and water resources (Wenzel et al., 1999; Lombi et al., 2000). For instance,
some varieties of Thlaspi and ecotype of Silene vulgaris have been found to be Cd accumulators; Larrea
tridendata, a desert inhabitant shrub, accumulates Cu, several wild species of Sutera accumulate Cr, and other
cultivated species accumulate Cd, Cr and Cu, maize and ambrosia accumulate Pb (Gardea Torresday et al., 2004).
However, researchers all over the world are searching new plant species susceptible to be used in
phytoremediation (Gardea Torresday et al., 2004). First, hyperaccumulators are usually specific for one
particular metal (Baker and Brooks, 1989), and are adapted to precise climate and soil conditions. Furthermore,
they cannot be managed as a conventional crop, have low biomass, and often a short life cycle. Therefore it
seems more reasonable to search for non hyperaccumulator plants showing good features for phytoremediation
and then transfer biotechnologically traits that make the modified plant even a more powerful tool than natural
hyperaccumulators.
Over the last few years on heavy metal tolerance and accumulation studies, the genetic modification
approach has gained significant momentum. The goal of genetic modification approach is to develop fast
growing, high shoot biomass plants with the metal accumulation traits of natural small biomass
hyperaccumulators: ‘engineered phytoremediators’ (Ow, 1996). The advantage of this technique is the relatively
short space of time and selective targeting of genes for improvement. With genetic engineering, plants can be
manipulated to accumulate, translocate and tolerate heavy metals, thus creating the ideal transgenic plant for
environmental cleanup in the shortest possible time (Pilon-Smits, 2005; Bennett, 2003; Persans et al., 2001). For
instance, genes can be isolated from metal hyperaccumulators and inserted into fast growing high biomass plant
species (Persans et al., 2001). It has been suggested that especially phytoextraction would become commercially
available if metal removal and tolerance properties of hyperaccumulator plants, such as Thlapsi caerulescens
(Brown et al., 1995; Bennett, 2003) or Pteris vittata (Ma et al., 2001), could be transferred into fast growing,
high biomass producing crop species. For example, most recently, Cd accumulation was enhanced when a
metallothionein gene from Silene vulgaris L. was overexpressed in the high biomass Nicotiana tabacum L.
(tobacco) (Gorinova et al., 2006).
Ultramafic rocks exposed to heavy tectonic activities usually contain high amounts of serpatine soils in
the Earth’s crust. Serpentine areas are generally characterized by high levels of heavy metals such as nickel,
cobalt and chromium. The soils derived from ultrafamic rocks lead to unusual and sparse associations of flora
that are tolerant of extreme environmental conditions such as high heavy metal contents. Serpentine soils,
"hotspots" of metallophyte endemics are a rich source of toxic trace elements. There are serpentine soils derived
from ultramafic rocks in various parts of the world. Serpentinized rocks are distributed all over the world viz.,
western north America; Newfoundland, Mount Albert in eastern Canada; Lizard peninsula, Wales and Scotland;
north-east Cuba; Portugal; Italy; Balkan peninsula; Turkey; topical far east; Central Brazil; New Caledonia;
south east Asia; Philippines; Japan; Zimbabwe; eastern Transvaal Loweveld of South Africa, New Zealand;
greenstone belts of western Australia (Proctor and Woodell, 1975; Sequeira et al., 1991). Significant exposures
of ultramafic rocks and soils are found in many parts of Turkey (Figure 1), although they are not such important
features of the geology of the eastern and south-eastern provinces. Notable areas include the central part of the
North-west (Kutahya and Balikesir provinces), the South-west between Antalya and Marmaris (Antalya and
Mugla provinces), the Amanus Mountains (Hatay and Adana provinces), regions of the eastern Taurus (north
and north-east of Mersin) and its extension into the Aladag massif (Nigde and Adana provinces), and numerous
areas in a band running generally north-eastwards for several hundred kilometers from near Adana to near
Erzincan (Figure 1). Other significant outcrops include several smaller areas near Ankara and in Canakkale
province. Soils developed on serpentine rocks cover a large area in Fındıkpınarı (Mersin, Turkey) where there
500

�are a large number of mines (e.g., chromium). Little is known about heavy metal contents of the natural plants
grown on Mersin-Findikpinari. Findikpinari is one of the plataeus used as a settlement place and has 1250 m
altitude (Orcan et al., 2004). Research area is on the Bolkar Mountains which is an interesting place from the
point of endemism (Orcan et al., 2004). The geological structure of the area is formed upper Crataceous
ultramorphic and serpentine. Common soil formations distinguished in the area as follows: brown forest soils,
reddish Mediterranean soils and brown calcareous soils (Orcan et al., 2004). Koleli et al., (2008) reported that
the maximum concentrations of metals in 11 soil samples collected from Mersin-Findikpinari (as dry mass)
were 909 mg kg-1 Cr, 3615 mg kg-1 Ni, 246 mg kg-1 Cu, 467 mg kg-1 Zn, 8.2 mg kg-1Cd and 111 mg kg-1 Pb.
Koleli et al., (2008) to determine hyperaccumulator species growing in serpentine soils in Findikpinari-Mersin,
total 123 plant species (members of 23 genera and 15 families) from 5 different sampling locations were
collected and analyzed for their total Cd, Cr, Cu, Ni, Pb, and Zn contents using an ICP-MS. The results indicate
that four plants species, mainly Thlaspi elegans Boiss. and Alyssum murale Waldst.&amp; Kit. contained Ni
concentrations up to 15693 and 13591 mg kg-1 Ni dry matter, respectively. Similarly, Anthemis cretica L. and
Sanicula europaea L. also contained Ni concentrations of 7741 and 4247 mg kg-1 DM, respectively. The
collected 755 specimens (52 family, 149 genera and 327 species) in Mersin-Findikpinari were identified by
Orcan et. al. (2004) in between 1997-2002. Orcan et al., (2004) reported that the largest family according to
number of the species is Fabaceae and the largest genus is Trifolium in this area.

Figure 1: Map of Turkey showing areas of ultramafic geology (in black) and of Mersin-Findikpinari (in red)
(from Reeves and Adiguzel, 2004)
The main objective of this study is to evaluate heavy metal accumulation ability of the different
plantspecies grown on Mersin-Findikpinari. The 26 specimens from the 755 specimens collected and identified
in between 1997-2002 by Orcan et. al. (2004) in Mersin-Findikpinari The plants were randomly selected to
evaluate heavy metal accumulation capacity..

Material and Methods
The shoots of identified plants were oven-dried at 70 °C for dry matter amount determination. Dried
shoot samples were ground and digested in 2 mL 30% H2O2 and 5 mL 65% HNO3 in sealed vessels of a
microwave (MarsXpress) apparatus. Each plant was replicated three times. Arsenic, Cd, Co, Cr, Cu, Mn, Ni, Pb,
Se and Zn concentrations were analyzed using an ICP-MS (Inductively Coupled Plasma-Mass Spectroscopy,
501

�Agillent 7500ce). Certified reference materials (SRM 1573A, SRM 1547) were also analyzed in order to check
the accuracy of the extraction technique used in the study.

502

�Family
Asteraceae
Asteraceae
Asteraceae
Boraginaceae
Caryophyllaceae
Iridaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Papaveraceae
Papaveraceae
Poaceae
Poaceae
Poaceae
Poaceae
Poaceae
Poaceae
Poaceae
Ranunculaceae

Plan
t no
689
699
85
2
111
4
141
744
740
758
208
741
739
747
759
72
775
712
720
722
707
728
708
713
5
Collection site
Purcu surroundings, under forest, rocky places
Purcu surroundings, under forest, rocky places
Cayirbogazi surroundings, waste places, open forest, under forest
Akarca Guzlesi-Findikpinari, roadside, under forest and open forest
Akarca Guzlesi-Findikpinari, roadside, under forest and open forest
Akarca Guzlesi-Findikpinari, roadside, under forest and open forest
Akarca Guzlesi-Findikpinari, roadside, under forest and open forest
Capurgedigi, surroundings, under forest
Purcu surroundings, under forest, rocky places
Capurgedigi surroundings, under forest and open forest
Devekoyagi surroundings, under forest and open forest
Findikpinari-Caglarca village, roadside
Cayirbogazi surroundings, under forest, rocky places, waste places
Capurgedigi surroundings, under forest and open forest
Capurgedigi surroundings, under forest and open forest
Cayirbogazi surroundings, waste places, open forest, under forest
Findikpinari, under forest
Bozon Guzlesi-Findikpinari, roadside, stony, rocky places
North of the Findikpinari, under forest, rocky slopes
Bozon Guzlesi-Findikpinari, roadside, stony, rocky places
Bozon Guzlesi-Findikpinari, roadside, stony, rocky places
Bozon Guzlesi-Findikpinari, roadside, stony, rocky places
Akarca Guzlesi-Findikpinari, roadside, under forest, stony places
Bozon Guzlesi-Findikpinari, roadside, stony, rocky places
Akarca Guzlesi-Findikpinari, roadside, under forest and open forest

503

Table 1: Family, genus, altitude, name of the collected site, and the collection date (from Orcan et. al., 2004)

Ranunculus ficaria L. subsp. calthifolius (Reichb.) Arc

Cynosurus echinatus L.

Festuca pinifolia (Hackel ex Boiss.) Bornm. var. pinifolia

Bramus diandrus Roth

Festuca jeanpertii (St.- Yves) F. Markgraf subsp. jeanpertii.

Briza humilis Bieb.
Poa speluncarum Edmondson
Aegilops neglecta Req. ex Bertol.

Corydalis solida (L.) Swartz subsp. tauricola Cullen &amp; Davis

Marrubium astracanicum Jacq.subsp.astracanicum
Purunella orientalis Bornm.
Prunella vulgaris L.
Lamium crinitum Montbret &amp; Aucher ex Bentham.
Nepeta nuda L. subsp. nuda
Fumaria kralikii Jordan

Lamium garganicum L. subsp. reniforme(Montbret &amp; Aucher ex Bentham) R. Mill

Name of the plant
Conyza bonariensis (L.) Cronquist
Crupina curipinastrum (Moris) Vis.
Anthemis aciphylla Boiss.var. aciphylla
Alkanna aucherana A.DC.
Silene dichotoma Ehrh. subsp. dichotoma
Crocus graveolens Boiss. &amp;Reuter
Scutellaria salviifolia Bentham
Micromeria carica P. H. Davis
Prunella vulgaris L.

Altitude,
m
1350
1350
1300-1500
1150
900-1150
900-1150
900-1150
1200-1300
1350
1200-1300
1800
1300-1400
1300-1500
1200-1300
1200-1300
1300-1500
1300-1350
1250
1300-1400
1250
1250
1250
900-1150
1250
900-1150

Collection
date
14.06.1998
14.06.1998
20.04.2002
14.03.2002
11.05.2002
14.03.2002
11.05.2002
28.06.1998
14.06.1998
09.05.1998
27.06.2002
15.07.1998
31.05.1998
09.05.1998
09.05.1998
20.04.2002
14.03.1999
01.06.1997
18.05.1997
01.06.1997
01.06.1997
01.06.1997
21.06.1997
01.06.1997
14.03.2002

�Findings
Research area is on the Bolkar Mountains which is an interesting place from the point of endemism of
Turkey. The collected 26 plants from different sampling locations have 26 genera and 8 families. Different 8
families were Asteraceae (3), Boraginaceae (1), Caryophyllaceae (1), Iridaceae (1), Lamiaceae (9), Papaveraceae
(2), Poaceae (7) and Ranunculaceae (1). In the identified 755 plant, the largest family according to number of the
species is Fabaceae and the largest genus is Trifolium. In the tested 26 plants, the largest family according to
number of the species is Poaceae (7). Table 1 shows family, genus, altitude, name of the collected site, altitude
and collection date of the tested plant samples.
Table 2 shows heavy metal concentrations in shoots of the investigated plant specimens. The highest As
(6), Co (10), Cr (46), Mn (548), Se (4) concentrations were Anthemis aciphylla Boiss. (Asteraceae). Manganese
concentration in Anthemis aciphylla Boiss. (Asteraceae) was higher than the critical concentration (300-500) in
plants according to Kabata-Pendias and Pendias (1992). Fumaria kralikii (Papaveraceae) has higher metal
content, except for Cd and Zn, than other plants and higher than normal concentration in plants according to
Kabata-Pendias and Pendias (1992). The highest Ni concentration was 115 mg kg-1 DM for Crocus graveolens
Boiss&amp;Reute (Iridaceae) and this value was higher than the critical concentration (10-100) in plants according to
Kabata-Pendias and Pendias (1992).
In the future, the identified 755 plants will be studied to evaluate heavy metal accumulation capacity
because of the research area is an interesting place from the point of endemism and remediation of contaminated
soils is essential for sustainable soil use. New selected metal hyperaccumulator plant may be genetically modify
and remediate metal-contaminated soils. But metal hyperaccumulator plants after treatment evaluated as
hazardous waste because of the higher concentration of the extracted metals. Therefore, further treatment of this
biomass is environmentally necessary.

504

�Scutellaria salviifolia Bentham
Micromeria carica P. H. Davis
Prunella vulgaris L.

Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Lamiaceae
Papaveraceae
Papaveraceae
Poaceae
Poaceae
Poaceae
Poaceae
Poaceae
Poaceae
Poaceae
Ranunculaceae
Asteraceae

9.

As
3
1
6
1
2
1
&lt;BD

Cd
1
1
1
3
1
1

Co
6
&lt;bd
10
1
2
8

Cr
24
1
46
8
11
31

Cu
29
11
29
18
16
21

Mn
335
47
548
88
235
189

Ni
40
3
67
35
13
115

505

&lt;bd
20
67
4
16
79
&lt;bd
&lt;bd
&lt;bd
&lt;bd
2
3
1
1
1
&lt;bd
3
5
21
126
1
1
&lt;bd
4
11
97
6
Lamium garganicum L. subsp. reniforme(Montbret &amp; Aucher ex Bentham) R. Mill
1
1
&lt;bd
7
17
96
24
Marrubium astracanicum Jacq.subsp.astracanicum
Purunella orientalis Bornm.
1
&lt;bd
1
3
16
80
9
Prunella vulgaris L.
1
2
1
5
15
101
7
&lt;bd
&lt;bd
&lt;bd
2
12
76
24
Lamium crinitum Montbret &amp; Aucher ex Bentham.
Nepeta nuda L. subsp. nuda
1
1
&lt;bd
3
12
158
6
Fumaria kralikii Jordan
1
6
6
26
31
247
30
&lt;bd
1
&lt;bd
3
17
9
107
Corydalis solida (L.) Swartz subsp. tauricola Cullen &amp; Davis
Briza humilis Bieb.
1
&lt;bd
&lt;bd
7
11
76
12
Poa speluncarum Edmondson
&lt;bd
&lt;bd
&lt;bd
2
11
67
4
Aegilops neglecta Req. ex Bertol.
&lt;bd
1
&lt;bd
4
6
49
6
&lt;bd
&lt;bd
&lt;bd
4
18
49
35
Festuca jeanpertii(St.- Yves) F. Markgraf subsp. jeanpertii.
Bramus diandrus Roth
&lt;bd
&lt;bd
&lt;bd
3
10
83
6
1
1
9
16
2
133
16
Festuca pinifolia (Hackel ex Boiss.) Bornm. var. pinifolia
Cynosurus echinatus L.
&lt;bd
1
&lt;bd
2
9
48
9
1
&lt;bd
3
19
24
137
60
Ranunculus ficaria L. subsp. calthifolius (Reichb.) Arc
Conyza bonariensis (L.) Cronquist
&lt;bd
1
&lt;bd
2
18
34
3
The highest value in plants
6
3
10
46
31
548
115
The lowest value in plants
1
1
1
1
2
3
1
Common concentrations in plants*
0.02-7
0.1-2.4
0.02-1
0.03-14
5-20
20-100
0.02-5
The critical concentration in the plants*
5-20
5-30
15-50
5-30
20-100
300-500
10-100
Hyperaccumulation threshold value
1000
100
1000
1000
1000
10000
1000
Table 2: Heavy metal concentrations of the tested plants (As, Cd, Co, Cr, Cu, Mn, Ni, Pb, Se and Zn), mg kg-1 DM *Kabata-Pendias (1992)

Name of the plant
Conyza bonariensis (L.) Cronquist
Crupina curipinastrum (Moris) Vis.
Anthemis aciphylla Boiss.var. aciphylla
Alkanna aucherana A.DC.
Silene dichotoma Ehrh. subsp. dichotoma
Crocus graveolens Boiss. &amp;Reuter

Family
Asteraceae
Asteraceae
Asteraceae
Boraginaceae
Caryophyllaceae
Iridaceae
13
2
7
22
20
3
17
8
20
35
7
6
9
4
14
18
19
3
9
6
35
2
0.2-20
30-300
1000

Pb
15
15
16
28
9
6
1
&lt;bd
1
1
1
1
1
1
1
3
1
&lt;bd
1
1
1
1
1
1
1
1
4
1
0.001-2
5-30
1000

Se
3
2
4
1
2
1

112
6
79
52
79
100
207
58
165
144
175
68
106
46
60
45
68
76
92
80
207
6
1-400
100-400
10000

Zn
137
179
84
79
65
104

�Acknowledgement
We thank Dr. Nermin Orcan and Dr. Riza Binzet for plant samples. We also thank Dr. Mehmet Arslan for critical reading of
the manuscript and helpful comments.

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507

�</text>
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                <text>Accumulation of Heavy Metals in Some Plants Grown on Serpentine Soils  of Mersin, Turkey</text>
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              <elementText elementTextId="23801">
                <text>Koleli, Nurcan
Demir, Aydeniz
Eke, Muzeyyen
Cakmak, Ozlem</text>
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          <element elementId="94">
            <name>Abstract</name>
            <description>A summary of the resource.</description>
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                <text>The purpose of this study was to determine hyperaccumulator species growing in  Findikpinari-Mersin. The soils derived from ultrafamic rocks lead to unusual and sparse  associations of flora that are tolerant to extreme environmental conditions such as high heavy  metal contents. As the geological structure, Mersin-Findikpinari has rocks containing  ultramafic and serpentine, but this site is one of the less studied areas. The 26 specimens of  total 755 plants identified systematically from Mersin-Findikpınari in between in 1997-2002  were randomly selected and studied whether hyperaccumulator or not. Twenty six plants  collected (members of 26 genera and 8 families) from different sampling locations were  analyzed for their total As, Cd, Co, Cr, Cu, Mn, Ni, Pb, Se and Zn concentrations using an  ICP-MS. A certified reference material (SRM 1573A, SRM 1547) was also analyzed to check  the accuracy of the used extraction technique. In the present study, Mn content (548 mg kg-1)  of Anthemis aciphylla Boiss. (Asteraceae) was higher than the critical Mn value (300-500 mg  kg-1) and Ni content (115 mg kg-1) Crocus graveolens Boiss&amp;Reute (Iridiceae) was higher  than the critical Ni value (10-100 mg kg-1) but unfortunately none of the plants studied was  hyperaccumulator.</text>
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PeerReviewed</text>
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                    <text>The Case, Problems and Solution Suggestions of The Greenhousing In
Biga
Hasan Kocabiyik
Çanakkale Onsekiz Mart University, Biga Vocational College
hkocabiyik@comu.edu.tr

Abstract : The greenhousing activity in Biga firstly started in an area of 500 m2 in
1985. In a review study, it was observed that total green housing area of 52.000 m2 and
42 greenhousing cooperations were reached. Greenhouses in Biga are in the structure of
bow roof, with plastic cover and high tunnel cold greenhouses. As the first investment
expenses are low and high productivity in a unit area, producers can make much money
at a short time. Due to all enterprises are small family cooperations, producers don’t pay
to workers. The distribution of productive power is regular in year and all enterprises
use dripping irrigation system. Lettuce is cultivated in greenhouses in winter. In spring,
summer and autumn cucumber, tomato and bean are cultivated. While some of the
produced yields are consumed in Biga and around villages, many parts of the yields are
marketed in Bandırma. The big problems in greenhousing cooperations; increasing of
entry prices, soil tiring, unconciously manurig and giving pesticides, structural
problems, insufficient of technique knowledge, packing and marketing. In this
presentation, the present case of greenhousing enterprises in Biga as alternative
incoming resource, its mainly problems and its possible solutions will be discussed.
Keywords: Biga, Greenhouse, family enterprise, plant production

Introduction
The first greenhouse in Biga was established in the village of Çeşmealtı in 1985 by a manufacturer.
Currenty in Biga 42 manufacturer is engaged in greenhouse and 52 decare greenhouse space and 155
greenhouses are present. These enterprises, 19 of them are in the center of the Biga and other
manufacturers are operating in the village. Biga plain, by the presence of 89.000 hectares watered, 640.000
hectares not watered, totally 729.000 hectares farmland is the most important district of Çanakkale from
the agricultural aspects (Çavuşgil and et al., 2005: 4).
According to the long years of climate data, average rainfall in the region is 765.7 mm and average
relative humidity is 74.5%. Annual average temperature is 14.2 0C, the highest temperature and lowest
temperature are 39.8 0C and -11.4 0C respectively. (Yavuz and et al., 2004:163)
The purpose of this research is the development of Biga on the greenhouse industry, to identify
the problems facing in the sector and to bring solutions to these problems. For this purpose, all owners of
greenhouse in Biga were discussed and all inventory owned by businesses were prepared.

Materials and Methods
In this study, all greenhouse enterprises in Biga and village were selected as the main material. A survey
comprising 18 questions were asked to business owners for the development of greenhouses and to identify
problems in greenhouses in Biga and villages. The data obtained from the sera owners were evaluated
through % rates and analyzed under the main heading.

174

�Results
In the inventory study, 42 greenhouse owners were interviewed in Biga and surrounding villages,
and the inventory information about greenhouse and greenhouse manufacturers were given.
Greenhouse Site Selection and Distribution of Greenhouse Enterprises
When all the ecological and economic factors that effect the greenhouse site selection taken into
consideration, definition of the location of the greenhouse can be made as follows: in autumn, winter and
spring months, it has high light intensity, with the winter is mild, good transport facilities, market demand
with cheap fuel, constant electricity, good-quality irrigation water and soil with heavy winds closed and
qualified workers can be found where appropriate are the places for greenhouse.(Sevgican et al., 1989 : 34).
19 of them in the greenhouse business (45.23%) are in the center of the Biga, 23 of them (54.76%) showed
activity in the village.
Observations Related to The Manufacturer

Experience of manufacturers in the industry ranged with 1 from 23. The average number of years
of dealing with greenhouse growers is 8. The educational level is often high school. For nine of them
(21.4%) the greenhouse business is a additional work. None of the greenhouse producer did not use credits,
incentives, support. New developments in the greenhouse can be examined in three groups: the greenhouse
structure improvements, new ways to reduce greenhouse labor and using the greenhouse except growing
plants (Yüksel et al., 2000: 233).
Although manufacturers are open to innovation of the manufacturers and to search of an
alternative open to innovation, they did not participate in activity about agricultural information
(conferences, symposia, etc.) in the area. Many manufacturers have no information on modern agricultural
practices such as using computer in greenhouses, soilless agriculture, organic agriculture, GAP,
EUREPGAP. Many manufacturers do not hold any record of fertilizers, drugs and yield. Hence the best
evaluation method for small fragmented land is greenhouse, farmers having small land turned to this sector.
Structural Features

Greenhouses are classified according to their size, organization forms, temperatures, roof shapes,
types of materials used in the skeleton and mask and also mobility status. According to their size
greenhouses are separated from big, medium and small greenhouses. If floor area greater than 1000 m2,
greenhouse is big greenhouse. If area between 100-1000 m2, greenhouse is medium greenhouse and if area
smaller than 100 m2, greenhouse is small greenhouse (Yüksel et al., 2000: 36).
Sizes of the greenhouse range in between 50 m2 to 3250 m2. The average size of the greenhouse
was found to be 344 m2. Total greenhouse area is 52 000 m2 and average greenhouse area per farm is 1238
m2. 6 greenhouse enterprises (14.28%) have block greenhouses and the other 36 enterprises (85.72%) have
individual greenhouses. Because of less snowfall, the block greenhouse was established in villages near the
sea.
Greenhouses in Biga are usually structured as a spring-roofed, plastic covered high-tunnel
shapping and cold. In individual greenhouses, base of greenhouse is thee oak piles and in block
greenhouses, base of greenhouse is a concrete pillar. In all skeletal material used in pipes but wooden
greenhouses profiles were not found. As greenhouse covering materials in all of the ultraviolet (UV) doped
with 3 to 3.5 years in life are using plastic sheeting. Because of lightweight, inexpensive, easily workable,
durable, good light transmission plastic was preferred.
Depending on the vegetable side elevation varies between 1.75 to 3.00 meters in vegetable
greenhouses. As the issue of width and length in the plastic greenhouses was relatively free movement,
usually width of 6-9 m and length of 30-60 m are used (Sevgican et al., 1989: 35). It was observed that the
width and length of greenhouse changing in a very large extent. The ridge height of greenhouse ranged 2 m
and 4 m. The side elevation in greenhouse cultivation is 2 m and also in seedling greenhouse is 1.5 m. In
cultivation greenhouse, width is 8.2 m in the 29 companies (69%) and height is 3.5 m in the 35 business
(83.3%) were observed. In individual greenhouses, width varies between 6 m and 12 m, and in block
greenhouse it varies between 20 m and 50 m.

175

�Ventilation and Moisture Control

For propose of natural ventilation is adequate, total area of roof windows should be between 1620 % of the greenhouse floor area and the openings should be set according to environmental conditions
(Yüksel et al., 2000: 71).
In four greenhouses (2.58%) of examined 155 greenhouses, the roof is ventilated, but ventilation
of the roof is not in others. 36 (23.22%) greenhouses were included in the side ventilation, and the
remaining 119 greenhouses (76.77%) are not included in the side ventilation. In all of the greenhouse has
been equipped with front and back air conditioning, hot summer weather when fully open front and back
surfaces have tried to improve the ventilation efficiency. Generally poor ventilation was observed.
Mandatory ventilation (ventilator-Extractor) has not been found. Ventilation was not getting control CO2
and humidity but in order to reduce greenhouse temperature. As a result of this, mildiyö and root rot disease
was observed to be effective in the greenhouse.
Heating &amp; Cooling

So the greenhouse effect of sunlight in the summer, especially growing greenhouse inside
temperature, outside air temperature may be higher than 50-10 0C. This reduction of assimilation in plants
and may lead to arrest. That is gained with assimilation of the plant material, may be less than that lost
through respiration (Yüksel et al., 2000: 128).
Heating is made in three greenhouses with a total area of 4470 m2 (8.6%). Heating is not to
ensure optimum temperature for the plant needed but only to ensure earliness in February and March.
Central heating system is used in the two companies, stove is used.one company.
Cooling system is not used in any business. Whereas the type of greenhouse warming is too important to be
cooled even in winter.
Irrigation and Drainage

Review of the greenhouse is used all the drip irrigation method. Taken from artesian well water is
filtered and purified by passing hidrosiklondan thus prevents clogging of drip breast. Chelate fertilizers are
added to the system with fertilizer tanks and soil pesticides are injected into the system.
Only one of the greenhouses used in the internal drainage system, both internal and external drainage was
not used in others.
Supply of Seed-Seedling

All greenhouses were used in the hybrid seeds. In recent years, the craftsmanship of local
producers as well as less healthy because they are directed to prepare the seedlings were observed. The
producers also were grafted on seedlings.
Production Pattern

784 m2 areas (1.5%) of review of the greenhouse were grown ornamental plants. In all of the other
greenhouse vegetables are grown. Type usually cucumbers in summer and lettuce(curly) -salad in winter
were grown. Moreover, purslane, eggplant, beans, peppers and tomatoes are grown by the manufacturer.
Fertilizing and Spraying

Soil pH values of Biga Plains ranged from 7.49 to 5.85. (Çavuşgil et al. 2005: 17).
The cucumber plants that are sensitive to acidity in the structure like the neutral or slightly alkaline soil
(Sevgican et al., 1989: 128).
Salad grows well in soils with pH = 6.0-7.0 , lettuce grows well in soils with pH = 5.5-7.0 (Aybak
et al. 2002: 46).
In review, 14 manufacturers (26.9%) analyzed their soil at least once. According to the results of
this greenhouse soil pH values were found to vary between 4.5 and 7, the average value was found to be
5.98.
176

�According to the analysis of the Biga Plains soil lime content is very low and many samples were found to
contain quantities of lime (Çavuşgil et al. 2005: 18).
Greenhouse manufacturers in the investigation they were often used on fertilizer: Before starting
the production of cucumber in soil , 10 ton/da of burnt.manure used were found. 15-15-15 compound
fertilizer as base fertilizer is used often. Drip irrigation system with ammonium nitrate, urea, potassium
nitrate, MAP and humic acids are used. In addition, some manufacturers are using magnesium nitrate and
ammonium sulfate fertilizers. Against micro-nutrient deficiency is the use of foliar fertilizers.
In greenhouses rest rotation is not applied. Usually removed product immediately the soil has been
processed with machines then the floor manure thrown and new products planted.
Examined business are to spray once the average 7-8 day. the production of cucumber is
commonly used systemic drugs in particular have been identified. Especially, cucumber production
commonly used systemic drugs were determined. Because of the hybrid seeds are used to fertilize itself,
hormones are not used. However, they are kind of some plant growth by the regulators were used. Some
producers are spraying gas engine, some spray back with a portion had been found.
Diseases and Pests

Cucumber and salads widely grown in greenhouses mostly determined diseases; cucumber angular leaf spot
disease (Pseudomonas syringae pv. Lachrymans), cucumber downy mildew (Pseudoperenospora cubensis),
the powdery mildew on cucumber and lettuce (Erysiphe cichoracearum), the gray mold disease on
cucumber and lettuce ( botrytis cinerea), white mold disease on cucumber and lettuce (Sclerotinia
sclerotiorum), mildiyö disease in lettuce (Bremia lactucae), such as bacterial and fungal diseases.
Most identified harmful pests are red spider (Tetranychus spp.), Leaf lice (Aphis gossypii), leaf gallery
beetle (Liriomyza spp.), Green worm (Heliothis armigera), thrips (Heliothrips haemorrhoidalis Bouche).
In the examined greenhouse, soil solarization is not done, and most were not known.
Harvesting, packaging and marketing
Cucumbers harvested are put into bananas boxes so that the reduced sweating and moisture loss.
15-20 pieces of Salad and lettuce in a big plastic bag were shown to be introduced to the market.
The biggest problem in marketing the market could not be a regular supply of goods because the
supply / demand balance against the manufacturer of the disruption caused by the instability of the prices
that have been identified.
Solution Proposals

Although it is aimed to enhance the efficiency in production, today's product quality 'and' food
security 'phenomenon has gained importance. Due to consumer demand for safe food, production
technology has also affected (Tüzel et al., 2004: 17).
Biga's greenhouse producers should give up being the small family businesses to be more healthy
and more modern facilities for certified products. In the coming years it will become necessary. It will be
possible to getting consolidation and institutionalization for small businesses.
The most important deficiency in Biga’s greenhouses is ventilation. Therefore length of the
greenhouse should be short and planting should not be frequent. Otherwise, due to beig the excess moisture
inside, fungal and bacterial diseases are steadily increasing. For an effective ventilation of the greenhouses,
length is more than 30 m and the greenhouse should be in the prevailing wind direction.
Producers have little information about fertilizers and fertilization, disease, pests, pesticides. Fort
his reason, it is recommended to take advice.
One of the biggest problem is marketing. To overcome this problem, new markets should find or
production planning should be done well.

References
Aybak, H. Ç. 2002 Salata ve Marul Yetiştiriciliği. Đstanbul: Hasad Yayıncılık Ltd. Şti. (In Turkish)
http://www.biga.gov.tr/biga.php?sayfa_id=102&amp;id=24&amp;1=1, 16.07.2008 (In Turkish)

177

�Çavuşgil, V. S., Ekinci, H., Özcan, H., Kavdır, Y., Yiğini, Y., Çolakoğlu, H. 2005 Biga Ovası Tarım Arazilerinin Bitki
Besin Elementi Đçerikleri Üzerine Bir Araştırma. Çanakkale Onsekiz Mart Üniversitesi, Yayınlanmamış Bilimsel
Araştırma Projesi. (In Turkish)
Sevgican, A. 1989 Örtüaltı Sebzeciliği. Yalova: TAV Yayınları (In Turkish)
Tüzel, Y. 2004 “Türkiye’de seracılığın gelişimi.” V. Sebze Tarımı Sempozyumu Bildiriler, 21-24 Eylül 2004,
Çanakkale. F. C. Kuzucu, C. Öztokat Kuzucu (editör). Çanakkale: Onsekiz Mart Üniversitesi, 16-18. (In Turkish)
Yavuz, M. Y., Altay, H., Erken, O., Çamoğlu, G. 2004 “Organik madde içeriği düşük topraklarda analiz sonuçlarına
göre uygulanan gübre dozunun Biga yöresinde yetiştirilen sanayi tipi domateste verim ve kalite parametrelerine
etkisi.”V. Sebze Tarımı Sempozyumu Bildiriler, 21-24 Eylül 2004, Çanakkale. F. C. Kuzucu, C. Öztokat Kuzucu
(editör). Çanakkale: Onsekiz Mart Üniversitesi, 162-164. (In Turkish)
Yüksel, A.N. 2000 Sera Yapım Tekniği. Đstanbul: Hasad Yayıncılık Ltd. Şti. (In Turkish)

178

�</text>
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                <text>The Case, Problems and Solution Suggestions of The Greenhousing In  Biga</text>
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                <text>Kocabiyik, Hasan</text>
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                <text>The greenhousing activity in Biga firstly started in an area of 500 m2 in  1985. In a review study, it was observed that total green housing area of 52.000 m2 and  42 greenhousing cooperations were reached. Greenhouses in Biga are in the structure of  bow roof, with plastic cover and high tunnel cold greenhouses. As the first investment  expenses are low and high productivity in a unit area, producers can make much money  at a short time. Due to all enterprises are small family cooperations, producers don’t pay  to workers. The distribution of productive power is regular in year and all enterprises  use dripping irrigation system. Lettuce is cultivated in greenhouses in winter. In spring,  summer and autumn cucumber, tomato and bean are cultivated. While some of the  produced yields are consumed in Biga and around villages, many parts of the yields are  marketed in Bandırma. The big problems in greenhousing cooperations; increasing of  entry prices, soil tiring, unconciously manurig and giving pesticides, structural  problems, insufficient of technique knowledge, packing and marketing. In this  presentation, the present case of greenhousing enterprises in Biga as alternative  incoming resource, its mainly problems and its possible solutions will be discussed.</text>
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                    <text>Comparison of Eleven Mathematical Models for describing the first
Lactation Curve of Holstein Cattle in Turkey
Đsmail Keskin
Department of Animal Science,
Faculty of Agriculture,
Selcuk University, 42075, Konya, Turkey
ikeskin@selcuk.edu.tr
Nazire Memmedova
Department of Animal Science,
Faculty of Agriculture,
Selcuk University, 42075, Konya, Turkey
naziramamedova@yahoo.com
Fatma Đlhan
Department of Animal Science,
Faculty of Agriculture,
Selcuk University, 42075, Konya, Turkey
fatmailhan@selcuk.edu.tr
Birol Dağ
Department of Animal Science,
Faculty of Agriculture,
Selcuk University, 42075, Konya, Turkey
bdag@selcuk.edu.tr
Fariz Mikailsoy
Department of Soil Science and Plant Nutrition,
Faculty of Agriculture,
Selcuk University, 42075, Konya, Turkey
farizm@selcuk.edu.tr

Abstract: In this study, eleven standard lactation curve models (Incomplete Gamma (WD),
Quadratic (Q), Cubic (C), Linear Hyperbolic Function (LH), Inverse Polynomial Function
(IP), Mixed Log (MIL), Exponential (WIL), Dhanoa (DH), Cobby and Le Du (CD),
Polynomial Regression (AS) and New Model (NM)) were used to predict a typical dairy cow
lactation derived as the average daily milk yield of 105 complete first lactations of HolsteinFriesian cows in one herd. Milk yield controls were made daily in this farm. Total milk yield
(TMY) was calculated from observation of daily milk yield. TMY was also predicted by using
11 different models. The total milk yields predicted by the models were very close to each
other and the differences between observation of TMY were not found statistically significant
(P&gt;0.05). The models were found to be adequate for estimation of milk yield.
Determination coefficients (R2) of the models ranged from 67.15 % to 86.68 %. In
comparison of the models, the TMY, peak yield (PY), peak time (PT), persistency (P), mean
square prediction error (MSPE), approximation error ( ε ), reliability criterion for estimating
trustworthiness of the determination coefficient ( σ ), standard error ( θ ) and Durbin-Watson
(DW) values were evaluated together.
Using the AS, WD and new developed NM models accurately predicted the milk
yield of Holstein cows.
Key Words: Holstein, Cows, Lactation Curve, Milk Yield, Mathematical Model

246

�Introduction
Turkey has 11.3 million head of cattle and 70 % of them are improved cattle and their crossbreeds. The
number of milked animals is 4.2 million and approximately 3 million of them are improved cattle and their
crossbreeds. The milk obtained from cows is 11.3 million tons and nearly 86 % of the milk production is
produced by improved cattle and their crossbreds. But the lactation milk yields is very low (i.e. for native breeds
1.3, for crossbreeds 2.7 and for improved cattle 3.9 tons) (TurkStat, 2007).
Producers aim to increase milk yield and decrease cost for a profitable dairy cattle production. Persistency
is one of the most important factors which determine milk production cost along the lactation. Milk yield begins
with calving and reach to highest level between 40 to 70 days and then continues to decrease along the lactation.
With decreasing of daily milk yield, the production cost begins to increase from day to day (Gengler, 1996;
Koçak and Ekiz, 2006). A mathematical model of the lactation curve provides summary information about dairy
cattle production, which is useful in making management and breeding decisions and in simulating a dairy
enterprise (Olori et al., 1999). In order to asses plausible forms of lactation curves, milk yield records collected
throughout the whole lactation are required. But most of the small and medium sized dairy farms in Turkey still
use classical milking systems. Milk yield is generally recorded monthly in these farms. The lactation curve
models enable them to evaluate lactation as a whole. So that the lactation curve shape is determined and
unbiased comparison methods among animals with incomplete lactation records for genetic evaluation purposes
can be practiced (Keown and van Vleck, 1973). Knowledge of the lactation curve allows prediction of total milk
production from partial production measured at several test days early in lactation (Goodal and Sprevak, 1985).
Animals with a high milk yield potential can be identified by using this information before the whole lactation is
completed. Also, lactation curves can be used for prediction of lifetime milk production from early lactation
traits (Dalal et al., 2004), culling, assessing nutritional and health status of animals (Duoduet, 1982; Souvant and
Fehr, 1975) and evaluating a suitable time to end milking (Chang et al., 2001).
The first mathematical model aimed to describe lactation curves was developed by Brody et al., (1923). It
was notified that this model was followed by the models reported out by Sika (1950), Nelder, (1966), Wood
(1967), Dave (1971) and Jenkins and Ferrel (1984) (Landete-Castillejos and Gallego, 2000). The Wood model
has been used in most lactation curve model studies, because it includes the basic features of lactation curves
with only three parameters a, b and c which allow the calculation of average yield, peak yield and peak time,
respectively. This has made the Wood model the most widely used function for the description of lactation
curves. Most of the alternative models are also based on the Wood model (Cobby and Le Du, 1978; Wilmink,
1987; Papajcsik and Bodero, 1988). However, some mathematical models have been proposed to describe the
regular shape of the lactation curve in dairy cows from partial or incomplete data (Neal and Thornley, 1983;
Goodal and Sprevak, 1984; Batra, 1986; Morant and Gnanasakthy, 1989; Dijkstra et al., 1997; Olori et al., 1999;
Vargas et al., 2000). Also these models provide analysing systemic changes in milk yield caused by
environmental factors (Goodall and Sprevak, 1985; Morant and Gnanasakthy, 1989) and determining the milk
production characteristics such as persistency (Gengler, 1996), peak yield and time to peak yield (Masselin et al.,
1987; Gipson and Grossman, 1990).
The objective of this study was to compare the suitability of WD, WIL, MIL, C, Q, DH, IP, CD, LH, AS
and NM models to the first lactation data of Holstein cows.

Materials and Methods
The data of this study was from the first lactation records of 105 Holstein cows raised in a private
enterprise in the Karapınar district (37o 42' K, 33o 35' D and 994 m above sea level) of the Konya Province in the
Central Anatolia Region of the Turkey. The data were collected from the first lactation records of the cows that
gave birth in 2004. They were machine milked twice daily and milking records were started 3th days of lactation.
There is a computer-based herd managing program in the enterprise and milk yield controls were made daily.
Average lactation length was 312±4.37 days. The experiment was carried out according to guidelines of Selçuk
University Faculty of Agriculture located in the Konya Province.
In the study, to explain lactation curves, eleven different empirical mathematical models were used
together and compared. These models are as follows:
(1) Incomplete Gamma (WD), (Wood, 1967):

Y( t ) = at b e − ct
(2) Quadratic (Q), (Dave, 1971):

Y( t ) = a + bt + ct 2
(3) Cubic (C),
247

�Y( t ) = a + bt + ct 2 + dt 3
(4) Exponential (WIL), (Wilmink, 1987):

Y( t ) = a + be − kt + ct (Which was fitted with the parameter k fixed at 0.61)
(5) Mixed log (MIL), (Guo and Swallve, 1995):

Y( t ) = a + bt 1 / 2 + c log t
(6) Polynomial Regression (AS), (Ali and Schaeffer, 1987):

Y( t ) = a + bt + ct 2 + d log t + e log t 2
(7) Cobby and Le Du (CD), (Cobby and Le Du, 1978):

Yt = a − bt − ae − ct
(8) Linear Hyperbolic Function (LH), (Bianchini, 1984):

Y( t ) = a + bt + c (1 / t )
(9) Inverse Polynomial Function (IP), (Nelder, 1966):

Y( t ) = t /(a + bt + ct 2 )
(10) Dhanoa (DH), (Dhanoa, 1981):

Yt = at bc e ( − ct )
(11) New developed model (NM):

Y( t ) = at b e

− ct −

d
t

For all models, Yt is the observed milk yield at day t,
a: is linked to milk yield at the beginning of lactation,
b: to the ascending phase before peak yield,
c: to the decreasing phase after peak yield,
d: parameters which characterize the shape of the curve
e: is the base of natural logarithm,
which were estimated from a nonlinear regression analysis using the Statistica program. The WIL model
has a total of four parameters, with k being exponent; following Wilmink (1987) a fixed value of k was used,
which was estimated at 0.61 in a preliminary analysis as the best fitting value for the herd mean data.
Subsequently the WIL model was considered as a three parameter curve in analysis of individual animals.
Persistency (P) was calculated as:
k

P (%) =

∑ (pi + 1) / pi
i =1

k

× 100

Where pi is the yield of the record i that starts at peak time and k is the record number from peak time to
the end of lactation (Sturtevant, 1986).
The parameters obtained were used to calculate the predicted yields in the original equations at above.
Residuals, defined as the absolute values of the difference between the predicted yield and real data of daily milk
yield, were calculated and then the mean square prediction error (MSPE) for each lactation curve fitted was
calculated and averaged for each model (Ruiz et al., 2000).
True peak yield (PY) was determined from the test day milk yield means for the 105 cows and true peak
time (PT) was determined as the average day on which daily milk yields were at their maximum value. Peak
time values of the models were calculated by equalizing the first partial derivations of the functions to zero, and
PY values were found for each cow by replacing PT values in the functions.
The Durbin-Watson statistics was used as a measure of first order positive autocorrelation to test whether
the residuals were randomly distributed (Grossman and Koops, 1988). DW was calculated for each lactation and
models.
Approximation error was calculated as:

ε=

100 n Yi − Y
∑
n i =1 Yi

Reliability criterion for estimating trustworthiness of the determination coefficient was calculated as:

248

�θ=

R2
n
1 − (R 2 ) 2

Standard error was calculated as:
n

∑ (Y

i

σ=

− Y)

i =1

n−m

The models were compared in respect of their MSPE, correlation between yields and residuals (RESC),
2
R , TMY, peak yield (PY), peak time (PT), persistency (P), ε , θ and σ .

Results and Discussion
Lactation curve parameters in Holstein cattle were given in Table 1. The parameter a, which expressed the
milk yield at beginning was 0.10 in IP model, and ranged from 12.71 to 25.81 among the other models.
Estimates of parameter a, were found in this study more higher than estimated for WD, Q, C, LHF and IP
models in Brown Swiss cows by Keskin and Tozluca (2004), for Q, C and WIL models in Simmental cows by
Çilek and Keskin (2008), for WD, IP and AS models in Holstein-Friesian cows by Olori et al., (1999); but less
than estimated for WD, MIL and AS models in Simmental cows by Çilek and Keskin (2008), for WD, MIL, WIL
and AS models in Brown Swiss cows by Keskin et al., (2009), for MIL and WIL models by Olori et al., (1999)
and very close to value estimated for WIL model in Holstein cows by Dědková and Němcová (2003).
Models*

Model parameters

a ± Sa

b ± Sb

WD
Q

15.34±0.428
22.04±0.419

0.161±0.0081
0.024±0.0038

C

20.53±0.649

AS

12.71±0.474

WIL

24.96±0.477

MIL

13.25±0.483

DH

20.38±0.759

LHF

25.81±0.438

0.062±0.0124
0.640±0.1099
26.064±1.1519
1.886±0.0831
49.468±11.7626
0.034±0.0015
0.032±0.0015

c ± Sc
0.0030±0.00012
0.0002±0.00001
-0.0004±0.00008
2.1894±0.34652

d ± Sd

0.00000054±0.000
0001
6.330±0.5275

e ± Se

1.022±0.1533

0.0307±0.00140
6.2888±0.25894
0.0020±0.00017

17.1840±0.74544
CD
25.44±0.439
18.3727±3.3172
0
IP
0.10±0.012
0.034±0.0009
0.0001±0.00000
5
NM
17.11±0.704
0.145±0.0118
0.0029±0.00013
0.153±0.0587
*
: WD: Incomplete Gamma, Q: Quadratic, C: Cubic, LH: Linear Hyperbolic Function, IP: Inverse Polynomial
Function, MIL: Mixed Log, WIL: Exponential, DH: Dhanoa, CD: Cobby and Le Du, AS: Polynomial
Regression and NM: New Model
Table 1. Estimates of the model parameters and their standard errors of eleven models
The highest estimate of parameter b was fixed in WD model, but the lowest estimate was fixed in DH
model. Estimates of parameter c were ranged from -17.1840 to 18.3727.
Estimated lactation curve parameters for this herd were generally different from the previous studies. It
may be due to raising in different environmental conditions or a result of management and administration in
different ways in terms of milk production. On the other hand it is well known that the Holstein breed is more
productive in temperate climatic zones and its milk production capacity may change by geographical regions.
249

�The parameter a expressing milk yield at the beginning and the b parameter indicating the speed of curve
increase must be higher and the c parameter meaning the speed of curve decrease must be lower in order to
obtain more milk production.
The lactation curves of Holstein cows are given in Figures 1. As seen in this figure, fit lines of WD, AS
and NM models are very close to the observed values. The total milk yield predicted by different models is very
close to observed total milk yield, and the differences between them, were not significant (P&gt;0.05).

250

�MSPE
5.95±0.321
6.11±0.300
5.75±0.712
4.90±0.256
7.04±0.370
5.99±0.329
7.90±0.542
6.57±0.356
6.88±0.370
7.57±0.418
5.67±0.315

RESC
-0.13
0.30
0.31
0.00
0.17
-0.17
0.61
0.04
0.13
-0.35
-0.17

Ɛ
14.00
13.71
14.12
12.74
15.33
14.29
15.90
14.95
15.13
15.78
13.93

σ
2.47
2.43
2.55
2.18
2.59
2.39
2.69
2.51
2.57
2.70
2.33

Ɵ
45.52
39.61
41.70
54.36
34.74
44.71
36.90
39.01
36.77
31.69
47.30

DW
0.849
0.776
0.804
0.949
0.716
0.833
0.737
0.775
0.732
0.673
0.877

Table 2. Comparison of the models for estimating total milk yield (TMY), peak yield (PY), time to peak yield (PT), Persistency (P), Correlation between yields and residuals
(RESC) and goodness-of-fit statistics (R2 and MSPE values)

Goodness of fit statistics
Models
TMY (l)
PY (l)
PT (day)
P (%)
R2 (%)
ns
bc
a
ns
WD
6407±150
24.50±0.368
72.84±17.20
99.7±0.01
76.17±0.014
Q
6370±148 ns
22.04±0.414d
0.00±0.000d
99.5±0.01 ns
75.23±0.013
20.53±0.649d
0.00±0.000d
99.6±0.03 ns
77.87±0.011
C
6338±145 ns
23.96±0.419c
56.14±3.267a
99.8±0.03 ns
80.65±0.011
AS
6370±148 ns
24.90±0.448bc
10.16±0.15c
99.9±0.05 ns
86.68±0.171
WIL
6370±148 ns
25.29±1.078bc
62.79±10.82a
99.8±0.04 ns
75.25±0.015
MIL
6370±148 ns
24.46±0.358bc
-46.17±11.66d
99.6±0.01 ns
63.59±0.025
DH
6372±148 ns
24.71±0.366bc
23.69±0.771c
99.9±0.38 ns
72.22±0.016
LHF
6370±148 ns
25.08±0.395bc
13.40±1.288c
99.8±0.03 ns
69.93±0.019
CD
6364±148 ns
IP
6335±147 ns
22.88±0.424b
29.31±1.287bc
99.9±0.02 ns
67.01±0.019
24.65±0.366bc
45.67±2.89ab
99.6±0.03 ns
76.88±0.014
NM
6309±159 ns
28.72±0.422a
69.38±4.85a
99.9±0.03 ns
Really
6369±149 ns
a, b, c, d
: The means within columns with different superscript are significantly different at P&lt;0.01
ns
: not significant.

�30

25

20
Milk Yield (l)

15

10

5

0
1

16 31

46 61

76

91 106 121 136 151 166 181 196 211 226 241 256 271 286 301
Day of Lactation

Data

AS

WD

WL

IP

LH

MIL

NM

DH

Q

C

CD

Figure 1. Shape of lactation curve according to the models
Total milk yield (TMY), peak yield (PY), time to peak yield (PT), Persistency (P), Correlation between
yields and residuals (RESC) and goodness-of-fit statistics (R2 and MSPE values) of the models are given in
Table 2. In this study, the differences between estimated and observed peak yields were found significant
(P&lt;0.01). But the differences between Q and C models’ peak yields were not significant and very close
predictions obtained with WD, WIL, MIL, DH, LHF, CD, IP and NM models. However the really peak yield
was found as 28.72, all models underestimated the peak yield. Generally the curves of real lactation data based
on daily milk yields are very fluctuant. But the estimated yields of the models are not fluctuant and also the
estimated peak yields do not show sharply increases as seen in real data. On the other hand it is expected that the
real lactation data of well managed herds do not fluctuate.
The peak time obtained from actual milk yield and the predicted PT from WD, AS and MIL models were
very close and the differences between them, were not significant (P&gt;0.05). But, the peak time of DH model was
negative and the peak times of Q and C models were estimated to be only zero. It is likely caused by the
decreasing curves from the beginning to the end of lactation for DH, Q and C models estimated by present data
as seen in Figure 1.
Persistency (P) values among all models were very close to each other and the differences between them,
were not significant (P&gt;0.05).
The P values, were found in this study were higher than values predicted for WD, Q, C, LHF and IP
models in Brown Swiss cows by Keskin and Tozluca (2004), for Q, C, WD, MIL, AS and WIL models in
Simmental cows by Çilek and Keskin (2008).
Higher determination coefficients for the used models show good fitting level of independent variables
for explaining dependent variables. For all models R2 value were estimated between 63.59 % and 86.68 %, it
was obtained the lowest value in DH (63.59) model, the highest in WIL (86.68) model. The best fitness was
obtained with WIL, it was followed by AS, but DH fitted worst. The lowest MSPE values were fixed in AS
model, then in WD model. The highest MSPE value was fixed in DH model. The R2 in this study, were lower
than R2 values, were notified for WD, Q, C, LHF and IP models in Brown Swiss cows by Keskin and Tozluca
(2004), for Q, C, WD, MIL, AS and WIL models in Simmental cows by Çilek and Keskin (2008), for WD, IP,
WIL, MIL and AS models in Holstein-Friesian cows by Olori et al., (1999).
MSPE values were higher than values, notified for Q, C, WD, MIL, AS and WIL models in Simmental
cows by Çilek and Keskin (2008).

252

�The lowest ε values were realized in AS model and it was followed by Q, NM and WD models. The
same condition was found for σ values, too. The highest θ values were estimated in AS model, it was followed
by NM and WD models. Autocorrelation values for all models were close to zero, indicating positive
autocorrelation which may pose problems with statistical inferences about the models.
Correlations between the residuals and observed milk yield (RESC) ranged between -0.35 (IP) to 0.61
(DH) for all models. Though estimated residuals generally increased with observed yields, there are a little
except for C, Q and DH. High daily yields being most difficult to predict while very low yields also caused
problems (Olori et al., 1999).

Conclusion
The TMY, PY, PT and P values in AS, WD and NM models were found very close to actual values, but
MSPE, RESC, ε and σ values were the lowest than actual. The highest R2 and θ values were found in these
models.
As the result of assessing of TMY, PY, PT, P, R2, MSPE, RESC, ε , σ , θ and DW statistics together, it
can be said that the using AS, WD and new developed NM models make possible of predicting milk yields,
close to actual values in Holstein cows at first lactation.

Acknowledgments
This research was funded in part by a grant from the University of Selcuk (BAP), The authors wish to thank the staff
of KAR-YEM AŞ, Konya, TURKEY.

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255

�</text>
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                <text>Comparison of Eleven Mathematical Models for describing the first  Lactation Curve of Holstein Cattle in Turkey</text>
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Memmedova, Nazire
İlhan, Fatma
Dağ, Birol
Mikailsoy, Fariz</text>
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                <text>In this study, eleven standard lactation curve models (Incomplete Gamma (WD),  Quadratic (Q), Cubic (C), Linear Hyperbolic Function (LH), Inverse Polynomial Function  (IP), Mixed Log (MIL), Exponential (WIL), Dhanoa (DH), Cobby and Le Du (CD),  Polynomial Regression (AS) and New Model (NM)) were used to predict a typical dairy cow  lactation derived as the average daily milk yield of 105 complete first lactations of Holstein-  Friesian cows in one herd. Milk yield controls were made daily in this farm. Total milk yield  (TMY) was calculated from observation of daily milk yield. TMY was also predicted by using  11 different models. The total milk yields predicted by the models were very close to each  other and the differences between observation of TMY were not found statistically significant  (P&gt;0.05). The models were found to be adequate for estimation of milk yield.  Determination coefficients (R2) of the models ranged from 67.15 % to 86.68 %. In  comparison of the models, the TMY, peak yield (PY), peak time (PT), persistency (P), mean  square prediction error (MSPE), approximation error ( ε ), reliability criterion for estimating  trustworthiness of the determination coefficient (σ ), standard error ( θ ) and Durbin-Watson  (DW) values were evaluated together.  Using the AS, WD and new developed NM models accurately predicted the milk  yield of Holstein cows.</text>
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                    <text>Effects of Arbucular Mycorrhizal Fungi Applications On Eggplant Seedling
Development
Levent Keskin
Department of Horticulture, Agricultural Faculty,
Selçuk University,TR-42031 Konya, Turkey
Mustafa Paksoy
Department of Horticulture, Agricultural Faculty,
Selçuk University,TR-42031 Konya, Turkey
paksoy@selcuk.edu.tr
Önder Türkmen
Department of Horticulture, Agricultural Faculty,
Selçuk University,TR-42031 Konya, Turkey

Abstract: The purpose of this research was conducted to determine the effects of arbuscular
Arbuscular Mycorrhizal Fungi applications (Gigaspora margarita and Glomus intraradices)
on seedlings growth parameters of Aydın siyahı, Faselis F1, Fabina F1, Topan, Vezir F1,
Kemer, Uzun patlıcan 50896, Uzun patlıcan 50516, Kara patlıcan 50710 and Pala eggplant
seedlings grown into the plastic greenhouse in Selçuk University. In results, hypocotil lenght
of Aydın siyahı and Kara patlıcan 59710, cothyledone width of Uzun patlıcan 50896,
cothyledone length, shoot lenght and diameter of Vezir F1, number of leaves of Aydın siyahı,
shoot fresh weight of Topan and Uzun patlıcan 50516, root fresh weight of Topan patlıcan
seedlings were found to be higher than the other eggplant cultivars. In the results of AMF
applications, hypocotil length, cothiledone width, cothyledone length, shoot length, number of
leaves, root fresh weight had been increased by G. margarita applications. Also, G.
intraradices applications had been increased the shoot fresh weight. In cultivar x Arbuscular
Mycorrhizal Fungi interactions, G. margarita exhibited better results on the hypocotil length
of seedlings of Aydın siyahı, cothyledone width of Uzun patlıcan 50896, shoot length and
number of leaves of Fabina F1, Kemer and shoot fresh weight of Uzun patlıcan 50516, root
fresh weight of Topan. Consequently, it was shown that it is necessary to determine the
proper cultivar materials and proper Arbuscular Mycorrhizal Fungi rage interactions to get a
better success in seedling development of eggplant.
Keywords: Glomus intraradices, Gigaspora margarita, eggplant cultivars, seedling
development.

Introduction
It is a more realistic approach in terms of environmental health and using natural sources to benefit from
the vegetable nutrient elements in an effective way instead of fertilizing with easy-taken nutrient elements to the
soil. It is a fact that one of the best ways of benefiting from the unit area is evaluating the microorganism activity
of soil. One of the microorganism formations that provide a better benefiting of soil for the plant is mycorrhizal.
Until now, it was thought that nutrient elements were taken by only roots. Recent researches have shown that
beside roots, vegetable nutrient elements are also mostly taken by fungus types which are called mycorrhizal and
produce plenty of hyphe (Ortaş et al. 2000). Researchers searched the effects of three different mycorrhizal fungus,
G.fasciculatum,G. monospromu and G. mossea, under field conditions by inoculating into tomato, eggplant and
pepper seedlings. The parameters that they used to measure the effects of mycorrhizal fungus on vegetable
development are; vegetable length, shoot fresh weight, total yield, fruit sizes and leaf length. Shoot fresh weight
for eggplant with G. mossea, G. monosporum and G. fasciculatum inoculations showed 47%, 28% and 29%
increases, respectively, and total yield of the same plant showed 60%, 43% and 7% increases. The most affective
fungus type among the three plant types inoculated to increase the development of the plant is G. mosseae.
However, G. fasciculatum is determined as the most effective fungus in the context of root colarisation of
eggplant and pepper plants. Mikorizal trials were conducted on a plenty of vegetable types among horticulture.
482

�Carrot (Smith and Read, 1997), tomato (Demir, 1998), pepper (Türkmen et al. 2005) are some of the examples of
these studies. The effects of mikorizal fungi on vegetable types can variable. This effect can be summed up in
the following way (Ortaş and Akpınar, 2004). Yield and fruit number increased for the eggplant inoculated by
AMF, and meaningful differences appeared among the mycorrhizal types in this increase. Especially, it was
determined that the spread of Verticillilum disease the eggplant inoculated by G.etinicatunium and G. margarita
spore were prevented (Matsubara and et al., 1995). According to Şen (2008), a positive effect was observed
through the G.intraradices application on eggplant seedling shoot length, shoot diameter, number of leaves,
shoot fresh weight, shoot dry weight, root fresh weight and root dry weight. All of these studies represent that
mycorrhizal is important for plant nutrition.
In this study, the purpose was to determine the effects of mycorrhizal specious (G.
intraradices and
G. margarita) on seedling development and growing up of the eggplant cultivar.

Material and Methods
This research was conducted to determine the effects of two Arbucular Mycorrhizal Fungi and ten
eggplant genotypes and cultivars in the greenhouse which belongs to Selçuk University Silifke Taşucu
Vocational High School. Aydın siyahı, Faselis F1, Fabina F1, Topan, Vezir F1, Kemer, Uzun patlıcan 50896,
Uzun patlıcan 50516, Kara patlıcan 50710 and Pala eggplant genotypes and cultivars were used as plant
materials. The soil mixture used in the trial was supplied with the mixture of garden soil and torf in the ratio of
1:1. Heat and humidity values were recorded data with microlog regularly. According to these records,
maximum temperature, average temperature and minimum temperature were measured 30 and 35 oC, 20 and 26
o
C, 16 and 22 oC. Relative humidity was measured between 55% and 56%. The soil mixture to grow seedlings
was sterilized at 121 oC in autoclave for two hours. The trial was planned and carried out in the consideration of
factorial trial pattern. This researched was designed with the notion of three replications, and in each parcel of
the research, there were 10 pots (10 plants) in each plot. Each pot had a 300 ml volume and had no drainage, and
pots were filled with soil mixture with was in the ratio of 1:1 soil and torf. The mixture including that had the
average 25 spore/g was added in 5 g to each pot in the same dept and at the same time with the seeds. The
nutruint solution melted in the pure water was added in 5 ml into each pot only once at the time of sowing. Three
seeds were planted into the each pot, and after they grew up, two of them were taken out. Each pot was watered
with pure water. Two Arbucular Mycorrhizal Fungi (G. intraradices and G. margarita) were applied in the trial.
In the control plants, mycorrhiza was not applied. The date of sowing seed which was the beginning of the
research was recorded. Hypocotyl length, cotyledon length, cotyledon width, period of real leaf appearance,
shoot length, shoot diameter, number of leaves, shoot fresh weight, root fresh weight were determined.
Determined research data were analyzed by Minitab program and means compared by Tukey Test.

Results and Discussion
The highest hypocotyls length was found out in Aydın siyahı and Kara patlıcan 50710 cultivar (2.89
mm) among the differences of cultivars in Table 1. The lowest hypocotyls lengths among the eggplant cultivars
were assessed as Fabina F1 (1.87 mm), Vezir F1 (1.90 mm), and Kemer (1. 95 mm) respectively. When the
effects of AMF applications on hypocotyls length were considered, the longest hypocotyls length was found out
in G. margarita (2.29 mm) and the shortest hypocotyls length was determined in G. Intraradices (2.23 mm).
The hypocotyls length of Aydın siyahı x G. margarita (3.23 mm) interaction was the highest and hypocotyls
length of Fabina F1 into G. margarita and G. intraradices was the lowest among to the AMF x eggplant
genotypes interactions. Consequently Al-Momany (1987) and Türkmen et al. (2008) have got similar results in
their researches.
The cotyledon length was observed in Vezir F1 cultivar (24.71 mm), and this was followed by Kara
patlıcan (22.77 mm). The shortest cotyledon length was found in Fabina F1 eggplant cultivars. The cotyledon
length in G. margarita (22.55 mm) took the first degree (Table 2). Menge and et al. (1978) appeared to support
our studies in their research results.
While the highest cotyledon width among the eggplant cultivar was observed in Uzun 50896 (11.19
mm) eggplant cultivar, the lowest cotyledon width was in Kemer eggplant (7.57 mm). According to the effects
of AMF applications on cotyledon width, the highest cotyledon width was detected in G. margarita applications
(8.58 mm), the lowest cotyledon width, on the other hand, was determined in G. intraradices (8.16 mm) (Table
3). Mosse (1981), Harley and Smith (1983) also found similar results with us.
When the effects of AMF applications on real leaves appearance duration were taken into consideration,
early real leaves appearance duration was determined in the control group (25.48 days). The longest real leaves
483

�appearance period appeared in G. intraradices as 26.59 days. As seen in interaction results, early real leaves
appearance was detected in control application with the eggplant cultivar of Aydın siyahı (24.96 days), Uzun
patlıcan 50896 (25.16 days), G. margarita Vezir F1 (25.31 days). In the longest G.intraradices application,
Aydın siyahı was determined as 27.56 days (Table 4). These findings are in accordance with the literature reports
which emphasize that proper cultivars specious interaction must be determined in order to get the purposed result
in AMF applications (Türkmen et al., 2008, Menge and et al., 1978).
While the highest shoot length among the eggplant cultivars was observed in Vezir F1 eggplant (18.61
cm), the lowest shoot length, on the other hand, was detected in Topan eggplant as 7.75 cm. When the effects of
AMF applications on shoot length were taken into consideration, the longest shoot was found out in G.
margarita with the length of 12.25 cm, but the shortest shoot length was assessed in the specious of G.
intraradices with the length of 10.48 cm (Table 5). In the research of Şen (2008), it was observed that the shoot
length of eggplant seedlings were between 13.62 and 11.48 cm. Al-Momany (1987) also found the same results.
While the highest shoot diameter among the eggplant cultivars was observed in Vezir F1 eggplant (5.75
mm), the lowest shoot diameter was detected in Kemer cultivar (3.91 mm). If the effects of mycorrhizal specious
on shoot diameter are examined, the highest shoot diameter is detected in the control application as 5.27 mm and
the lowest shoot diameter is determined in G. intraradices as 4.67 mm (Table 6). Although, Tinker (1980) and
Şen (2008) reported that seedling shoot diameters were increased through the AMF applications. According to us,
this difference is caused by the differences between the AMF specious and the cultivars.
While the most number of leaves was found out in Aydın Siyahı (7.52), the fewest number of leaves
was found out in Uzun patlıcan 50516 (7.08). When the effects of AMF applications on the number of leaves
were examined, the most number of leaves was determined in G.Margarita (7.64) and the fewest number of
leaves was observed in G. intraradices (6.87) (Table 7). Şen (2008) recorded an increase in the number of leaves
through the G.intraradices on eggplant seedlings (4.97), but the number of leaves for the eggplant seedlings on
which mycorrhizal wasn’t applied, it was found as 3.84. The study results of Harley and Smith (1983) are
parallel to our study results.
The highest shoot fresh weight among the eggplant cultivars was remarked in Topan (24.45 g) and
Uzun Patlıcan 50516 types (24,45), whereas the lowest shoot fresh length was in Fabina F1 (17.94 g) and Faselis
F1 (17.93 g). When the effects of mycorrhizal on shoot fresh weight were taken into consideration, the heaviest
shoot fresh weight was detected in G. intraradices (22.68 g). The lowest shoot fresh weight was found in the
control group (17.60 g) (Table 8). Al-Momany (1987) was discovered that the shoot fresh weight for eggplant
increased with inoculations of G.mossea, G.monosporum and G.fasciculatum in the ratios of 47%, 28% and 29%,
respectively, and the yield for the same plant increased in the ratios of 60%, 43% and 7%, espectively. Şen
(2008) found the increase in terms of shoot fresh weight.
While the highest root fresh weight among eggplant cultivar was remarked in Topan genotype (8.80 g),
the lowest root fresh weight was determined in Faselis F1 cultivar (3.35 g), Uzun patlıcan 50516 (2.91 g) and
Kemer (3.04 g) cultivar. When the effects of mycorrhizal on root fresh weight were examined, the harvest root
fresh weight was noted as G. margarita (6.18 g) and the lowest root fresh weight was determined in the G.
intraradices group as 4.67 g (Table 9). Şen (2008) obtained the increase in terms of root fresh weight through
the application of G. intraradices. Onuğur and Demir (1988) concluded that shoot and root fresh and dried
weights increased through the AMF applications.

Conclusion
In this research, according to eggplant materials, in the context seedling development change
parameters, Aydın siyahı and Kara patlıcan 59710 in terms of hypocotyls length, uzun patlıcan 50896 in terms
of hypocotyls width, Vezir F1 in terms of hypocotyls l length enght, shoot l length, and shoot diameter, Aydın
siyahı in terms of the number of leaves, Topan and Uzun patlıcan 50516 in terms of shoot fresh weight, Topan
eggplant in terms of root fresh weight were found to be superior cultivar than the others. It was determined that
in the context of AMF applications, through the G. margarita application hypocotyls length, cotyledon width,
cotyledon length, shoot length, number of leaves, root fresh weight increased, the G. intraradices application, on
the other hand, increased shoot fresh weight. To sum up, it is discovered that it is necessary to determine the
proper vegetable materials and proper AMF specious interactions to get a better success in vegetable
development of eggplant through AMF applications.

484

�References
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pepper in a field soil. Dirasat (Jordon) 14:11, 161-168.
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and their role in resistance. Doctora Thesis, Ege University. Đzmir
Harley, J.L., Smith, S,E. (1983). Mycorrhizal Symbiyosis Academic Press. London UK.
Matsubara, Y., Harada, T., Yakuwa, T. (1995). Effect of inokulum density of Vesicular Arbuscular Mycorrhizal Fungal
spores and addition of carbonized material to bed soil on growth of welshonion seedlings. Journal of the Japanese Society
for Horticultiral Science 64(3): 549-554.
Menge, J.A., Johnson, E.L.V., and Platt R.G. (1978). Partial substitution of Mycorrhizal Fungi for phosphorus fertilization in
the greenhouse culture of citrus. Soil Science Society of American Journal, 42: 926-930.
Mosse, B. (1981). Vesicular- Arbuscular Mycorrhiza research for tropical agriculture research bulletin. Hawaii Instıtute of
Tropical Agriculture and Human Resources. 82p.
Onoğur, E., Demir, S. (1988). Bazı kültür bitkilerinde Vesicular- Arbuscular Mycorrhiza (VAM) oluşumu ve bunun bitki
gelişimi ve dayanıklılıktaki rolü üzerinde araştırmalar. TUBITAK Tarım ve Ormancılık Grubu Proje No: TOGTAG 1506.
Ortaş, Đ., Kaya, Z., Sarı, N., Gök, M., Çakmak, Đ., Almaca, A., Ergün, B., Ortakcı, D., Köse, Ö., Ercan, S., Bolat, H., (2000).
Doğal bir gübre olan mikoriza uygulamasının bitkisel verim ve mineral gübre tasarrufundaki rolü ve mikorizaya bağımlılık
duyan kültür bitkilerinin seleksiyonu. DPT Toprak Biyoteknolojisi Araştırma Projesi Kesin Sonuç Raporu (Proje No:96 K
120-580). Adana.
Ortaş, Đ., Akpınar, Ç. (2004). Use and Importance of Agriculture Mikoriza. Turkey 3. the National Congress of Agricultural
Fertilizer Industry Environment, 861-876, 11-13 October. Tokat
Smith, S.E., Read, D.J. (1997). Mycorrhizal Symbiosis. second edition. Combridge Academic Press.
Şen, Ö. (2008). Tuz Stresi Altında Yetiştirilen Patlıcan Fidelerinin Gelişimi ve besin elementi Đçerikleri Üzerine Arbuscular
Mikorizal Fungus (Glomus intraradices) Uygulamalarının Etkisi. Master Thesis, University Of Selcuk, Faculty of
Agriculture, Dept. of Horticulture, Konya
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Agriculture (Eds, Khosewenek, F,E et al). ASA-CSSA- SSSA, Madison, USA.
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seedling development and nutrient content of pepper grown under saline soil conditions. Journal of Biological Sci. 5 (5): 568
574.
Türkmen, Ö., Şensoy S., Demir, S., Erdinç, C. (2008). Effect of two different AMF species on growth and nutrient content of
pepper seedlings grown under moderate salt stres. African Journal of Biotecnology 7(4) : 394-396.

Cultivars
Fabina F1

Control
1,95±0.010I

G. intraradices
1,84±0.032m

G. margarita
1,82±0.011m

Means
1,87±0.061E

Faselis F1
Vezir F1
Pala
Kemer
Topan
Aydın Siyahı
Uzun patlıcan 50516
Kara patlıcan 50710
Uzun patlıcan 50896

2,13±0.005gh
1,96±0.005kI
2,04±0.005ı-k
2,02±0.010j-l
2,11±0.015g-ı
2,77±0.011d
2,41±0.011f
2,87±0.011c
2,38±0.068 f

2,01±0.010j-l
2,05±0.005h-j
2,03±0.010j-l
1,80±0.020m
2,05±0.011h-j
2,65±0.010e
2,18±0.026g
2,97±0.010b
2,76±0.020d

2,12±0.340g-ı
1,70±0.052n
2,03±0.005j-l
2,03±0.011j-l
2,02±0.005j-l
3,23±0.041a
2,44±0.020f
2,84±0.020cd
2,67±0.030e

2,09±0.059D
1,90±0.158E
2,03±0.007D
1,95±0.114E
2,06±0.043D
2,89±0.267A
2,34±0.123C
2,89±0.061A
2,60±0.173B

Means
S x 0.01

2,26±0.324B
2,23±0.393C
0.01354
S
(cultivars) =
x 0.01 (Mycorrhiza) =0.004282 S

2,29±0.473A

x

0.01 (cultivars x Mycorrhiza)

=0.01354

Table 1. The effects of AMF applications on hypocotyl length of eggplant cultivars (mm).

485

�Cultivars
Control
G. intraradices
Fabina F1
21.07±0.1j-o
17.33±0.9p
Faselis F1
20.72±0.3k-o
20.35±0.3m-o
Vezir F1
25.47±0.3a
24.49±0.2a-c
Pala
22.52±0.1e-h
20.25±0.0no
Kemer
21.11±0.0i-o
18.61±0.2p
Topan
22.41±0.0f-ı
21.64±0.0h-m
Aydın Siyahı
23.08±0.3d-g
21.21±0.0ı-o
Uzun patlıcan 50516
21.72±0.0h-l
20.00±0.0o
Kara patlıcan 50710
23.06±0.0d-g
22.20±0.6f-j
Uzun patlıcan 50896
15.96±0.3q
24.77±0.7ab
Means
21.72±2.3B
21.09±2.2C
S x 0.01 (cultivars) =0.2008 S x 0.01 (Mycorrhiza) =0.06351 S

G. margarita
20.44±0.0l-o
21.58±0.1h-m
24.16±0.0b-d
22.56±0.1e-h
21.46±0.0h-n
23.32±0.0c-f
23.29±0.5c-f
21.81±0.5g-k
23.05±0.5d-g
23.79±0.1b-e
22.55±1.1A

x

Means
19.61±1.7F
20.88±0.6DE
24.71±0.6A
21.78±1.1B-D
20.40±1.3EF
22.46±0.7BC
22.53±1.0BC
21.18±0.9DE
22.77±0.5B
21.51±4.2CD

0.01 (cultivars x Mycorrhiza) = 0.2008

Table 2. The effects of AMF applications on kolitedon length of eggplant cultivars (mm).

Cultivars
Control
G.intraradices
Fabina F1
7.46±0.07n-p
7.11±0.03p
Faselis F1
8.61±0.04e-g
7.95±0.06ı-m
Vezir F1
9.28±0.06d
9.20±0.02d
Pala
8.04±0.01h-l
7.36±0.05n-p
Kemer
7.67±0.07l-o
7.25±0.10op
Topan
8.07±0.04h-l
7.49±0.06m-p
Aydın Siyahı
8.48±0.05e-h
7.40±0.07n-p
Uzun patlıcan 50516
8.62±0.25e-g
8.22±0.04g-j
Kara patlıcan 50710
8.95±0.04de
8.62±0.07e-g
Uzun patlıcan 50896
9.96±0.09c
11.03±0.04b
Means
8.51±0.73A
8.16±1.16B
S x 0.01 (Cultivars) =0.07348 S x 0.01 (Mycorrhiza) =0.02324 S

G. margarita
7.71±0.14k-o
8.42±0.15f-ı
8.860±0.05d-f
7.51±0.076m-p
7.81±0.066l-o
8.34±0.055g-ı
8.17±0.068g-k
8.03±0.104h-l
8.42±0.026f-ı
12.58±0.52a
8.58±1.415A

x

Means
7.42±0.27G
8.32±0.30CD
9.11±0.19B
7.63±0.31E-G
7.57±0.26FG
7.97±0.38D-F
8.01±0.48DE
8.29±0.29CD
8.66±0.23C
11.19±1.17A

0.01 (Cultivars x Mycorrhiza)=0.07348

Table 3. The effects of AMF applications on kolitedon width of eggplant cultivars (mm).

Cultivars
Control
G. intraradices
Fabina F1
25.75±0.1 c-e 26.40±0.2b-d
Faselis F1
25.65±0.2c-e
27.23±0.2ab
Vezir F1
25.81±0.0 c-e 26.37±0.0b-d
Pala
25.39±0.1de
25.71±0.6c-e
Kemer
25.41±0.5de
25.93±0.1c-e
Topan
25.42±0.5de
25.93±0.1c-e
AydınSiyahı
24.96±0.2e
27.56±0.1a
Uzun patlıcan 50516
25.68±0.0cde
27.28±0.0ab
Kara patlıcan 50710
25.46±0.0de
25.54±0.0de
Uzun patlıcan 50896
25.16±0.0e
27.25±0.0ab
Means
25.48±0.3B
26.59±0.7A
S x 0.01 (Cultivars) = Ö.D. S x 0.01 (Mycorrhiza) =0.0499 S x

G. margarita
25.68±0.1c-e
25.71±0.1c-e
25.31±0.0e
25.40±0.1e
25.64±0.6c-e
25.64±0.6c-e
25.41±0.6de
25.35±0.0e
25.67±0.2c-e
24.99±0.0e
25.49±0.3B

Means
25.94±0.37
26.19±0.79
25.83±0.46
25.50±0.40
25.95±0.53
25.66±0.50
25.97±1.25
26.10±0.88
25.55±0.15
25.80±1.08

0.01 (Cultivars x Mycorrhiza)=0.1579

Table 4. The period of real leaves appearance in AMF applications of eggplant cultivars.

Cultivars

Control

G.intraradices

G. margarita

Means

Fabina F1
Faselis F1
Vezir F1
Pala
Kemer

12.49±0.46 gh
15.29±0.10d
17.42±0.06b
10.71±0.07k
7.71±0.25no

9.47±0.03I
16.44±0.12c
18.06±0.05b
10.93±0.10jk
6.77±0.04pq

9.29±0.03 I
15.68±0.07cd
20.36±0.19a
11.93±0.16hı
8.77±0.13Im

10.41±1.5E
15.80±0.51B
18.61±1.34A
11.19±0.57D
7.75±0.87G

Topan

7.33±0.05o-q

6.62±0.11qr

7.50±0.05op

7.15±0.40G

486

�Aydın Siyahı
Uzun patlıcan 50516
Kara patlıcan 50710

7.51±0.15op
7.04±0.06o-q
12.67±0.19f-h

8.41±0.03mn
5.90±0.10r
11.63±0.20u

Uzun patlıcan 50896
13.06±0.02e-g
10.64±0.32k
Means
11.12±3.54B
10.48±3.93C
S x 0.01 (Cultivars) = 0.04 S x 0.01 (Mycorrhiza)=0.12 S

x

9.55±0.38L
13.31±0.39ef
13.49±0.61e

8.49±0.91F
8.75±3.46F
12.59±0.87C

12.63±0.07f-h
12.25±3.69A

12.11±1.13C

0.01 (Cultivars x Mycorrhiza)=0.12

Table 5. The effects of AMF applications on shoot lengths (cm) of eggplant cultivars

Cultivars
Fabina F1
Faselis F1
Vezir F1
Pala
Kemer
Topan
Aydın Siyahı
Uzun patlıcan 50516
Kara patlıcan 50710

Control
4.19±0.02 no
5.42±0.07f-h
5.55±0.10e-g
4.99±0.11ı-k
4.18±0.03no
5.26±0.05g-ı
6.37±0.27a
4.9±0.08k-m
6.26±0.07ab

G. intraradices
3.90±0.13op
5.16±0.05h-j
5.69±0.01c-f
4.78±0.02j-l
3.81±0.17op
4.55±0.05l-n
4.69±0.01k-m
3.85±0.12 op
4.60±0.01k-m

G. margarita
4.45±0.11l-n
6.07±0.02a-c
6.03±0.02a-d
5.68±0.08d-f
3.74±0.11p
4.68±0.10k-m
4.75±0.04kl
4.35±0.30mn
5.39±0.01f-h

Means
4.18±0.25FG
5.55±0.41A-C
5.75±0.21A
5.15±0.41DE
3.91±0.02G
4.84±0.33E
5.27±0.83CD
4.30±0.40F
5.41±0.72B-D

Uzun patlıcan 50896
Means

5.88±0.02b-e
5.27±5.281A

5.16±0.03h-j
4.61±0.60 C

6.11±0.03ab
5.13±0.81B

5.71±0.43AB

S

x

0.01 (Cultivars)=0.06

S

x

0.01 (Mycorrhiza) =0.02

S

x

0.01 (Cultivars x Mycorrhiza )

=0.06

Table 6. The effects of AMF applications on shoot diameters (mm) of eggplant cultivars

Cultivars
Fabina F1
Faselis F1
Vezir F1
Pala
Kemer
Topan
Aydın Siyahı
Uzun patlıcan 50516
Kara patlıcan 50710

Control
7.23±0.03j-l
7.40±0.05f-j
6.91±0.01mn
7.31±0.09ı-l
7.35±0.05g-k
7.22±0.02j-l
7.60±0.04d-f
7.11±0.12Im
7.60±0.04d-g

G.intraradices
6.62±0.11qp
6.81±0.07no
7.53±0.03d-h
6.91±0.01mn
6.67±0.04op
6.58±0.02p
7.32±0.02h-l
6.56±0.03p
6.92 ±0.02mn

Uzun patlıcan 50896
7.46±0.03e-ı
6.74±0.01n-p
Means
7.31±0.21B
6.87±0.31C
S x 0.01 (Cultivars) =0.03 S x (0.01 (Mycorrhiza) =0.01 S

x

G. margarita
7.95±0.13a
7.87±0.06ab
7.50±0.05d-ı
7.66±0.03c-e
7.86±0.03a-c
7.69±0.02b-d
7.66±0.01c-e
7.55±0.05d-g
7.57±0.02d-f

Means
7.26±0.58B-D
7.36±0.46AB
7.31±0.30BC
7.29±0.32B-D
7.29±0.51B-D
7.16±0.47C-E
7.52±0.15A
7.08±0.43E
7.36±0.33AB

7.16±0.03kl
7.64±0.22A

7.12±0.31DE

0.01 (Cultivars x Mycorrhiza)=0.03

Table7. The effects of AMF applications on the number of leaves of eggplant cultivars.

Cultivars
Fabina F1
Faselis F1
Vezir F1
Pala
Kemer
Topan
Aydın siyahı
Uzunpatlıcan50516
Kara patlıcan 50710
Uzun patlıcan 50896
Means

Control
16.16±0.125I
15.61±0.061I
20.42±0.094ı
15.68±0.325I
17.39±0.138k
22.38±0.023gh
13.98±0.553n
19.86±0.140ıj
19.28±0.160j
15.20±0.172m
17.60±2.647C

G. intraradices
18.17±0.186k
20.57±0.064ı
25.04±0.010d
16.38±0.637I
25.16±0.485cd
24.30±0.110de
23.87±0.107ef
26.07±0.030bc
22.12±0.046h
25.13±0.034d
22.68±3.192A

G. margarita
19.51±0.076j
17.62±0.026k
19.23±0.015j
17.48±0.425k
27.02±0.540a
26.67±0.026ab
24.42±0.068de
27.43±0.034a
19.85±0.157u
23.11±0.036fg
22.24±3.824 B

Means
17.94±1.466 E
17.93±2.161 E
21.56±2.655 C
16.51±0.889 F
23.19±4.438 B
24.45±1.859A
20.75±5.096 D
24.45±3.496A
20.41±1.306D
21.14±4.544C

S x 0.01 (Cultivars) = 0.14 S x 0.01 (Mycorrhiza) =0.04 S x 0.01 (Cultivars x Mycorrhiza) =0.14
Table 8. The effects of AMF applications on shoot fresh weight in eggplant cultivars
487

�Cultivars
Control
G. intraradices
Fabina F1
7.50±0.02 cd
6.08±0.03ef
Faselis F1
3.45±0.06 g-j
3.15±0.03h-j
Vezir F1
6.14±0.01ef
4.26±0.01gh
Pala
5.66±0.03f
6.51±0.01def
Kemer
2.61±0.04jk
2.41±0.03 jk
Topan
9.33±0.10ab
6.79±0.01 d-f
Aydın Siyahı
6.98±0.02de
3.41±0.06 g-j
Uzun patlıcan 50516
2.92±0.07ı-k
1.85±0.17 k
Kara patlıcan 50710
7.49±0.01cd
6.24±0.01ef
Uzun patlıcan 50896
7.52±0.03cd
6.01±0.03ef
Means
5.96±2.19B
4.67±1.79 C
S x 0.01 (Cultivars) =1.04 S x 0.01 (Mycorrhiza) =0.06 S

G. margarita
4.47±0.02 g
3.45±0.05 g-u
6.14±0.01ef
6.30±0.01ef
4.11±1.31gh
10.29±1.0 a
5.86±0.01ef
3.98±0.02g-ı
8.96±0.05b
8.30±0.02bc
6.18±2.28A

x

Means
6.01±1.31CD
3.35±0.15F
5.51±0.94DE
6.15±0.38C
3.04±1.03F
8.80±1.65A
5.41±1.58E
2.91±0.92F
7.56±1.17B
7.27±1.01B

0.01 (Cultivars x Mycorrhiza)

=0.18

Table 9. The effects of AMF applications on root fresh weight in eggplant cultivars (g).

488

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Paksoy, Mustafa
Türkmen, Önder</text>
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                <text>The purpose of this research was conducted to determine the effects of arbuscular  Arbuscular Mycorrhizal Fungi applications (Gigaspora margarita and Glomus intraradices)  on seedlings growth parameters of Aydın siyahı, Faselis F1, Fabina F1, Topan, Vezir F1,  Kemer, Uzun patlıcan 50896, Uzun patlıcan 50516, Kara patlıcan 50710 and Pala eggplant  seedlings grown into the plastic greenhouse in Selçuk University. In results, hypocotil lenght  of Aydın siyahı and Kara patlıcan 59710, cothyledone width of Uzun patlıcan 50896,  cothyledone length, shoot lenght and diameter of Vezir F1, number of leaves of Aydın siyahı,  shoot fresh weight of Topan and Uzun patlıcan 50516, root fresh weight of Topan patlıcan  seedlings were found to be higher than the other eggplant cultivars. In the results of AMF  applications, hypocotil length, cothiledone width, cothyledone length, shoot length, number of  leaves, root fresh weight had been increased by G. margarita applications. Also, G.  intraradices applications had been increased the shoot fresh weight. In cultivar x Arbuscular  Mycorrhizal Fungi interactions, G. margarita exhibited better results on the hypocotil length  of seedlings of Aydın siyahı, cothyledone width of Uzun patlıcan 50896, shoot length and  number of leaves of Fabina F1, Kemer and shoot fresh weight of Uzun patlıcan 50516, root  fresh weight of Topan. Consequently, it was shown that it is necessary to determine the  proper cultivar materials and proper Arbuscular Mycorrhizal Fungi rage interactions to get a  better success in seedling development of eggplant.</text>
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                    <text>Use of Computer Simulation Technology in
Engineering Education
Ramazan Kayıkcı
Faculty of Technology,
Sakarya University, 54187 Sakarya, Turkey
rkayikci@sakarya.edu.tr

Abstract: The function of the engineering profession is to manipulate materials, energy, and
information, in that way creating benefit for humankind. To perform this effectively, engineers
must have knowledge of nature that goes beyond plain theory that is traditionally gained in
educational laboratories. In recent years, however, the environment of these laboratories has
changed. This paper describes the use of simulation technology in engineering education. In
particular, the paper considers materials and metallurgical engineering field which involves
many high temperature materials processing such as castings and solidification. In this study,
an example of use of simulation technique to demonstrate the effects of casting geometry on
solidification times of three different geometries in sand casting process been presented.

Introduction
The overall goal of engineering education is to prepare students to practice engineering and, in particular,
to deal with the forces and materials of nature. Thus, from the earliest days of engineering education,
instructional laboratories have been an essential part of undergraduate and, in some cases, graduate programs.
Indeed, prior to the emphasis on engineering science, it could be said that most engineering instruction took
place in the laboratory. As an example, in surveys of the articles published in the Journal of Engineering
Education from 1993 to 1997, it was found that only 6.5 percent of the papers used laboratory as a keyword.
From 1998 to 2002, the fraction was even lower at 5.2 percent (Wankat 2004).
Laboratory instruction has been complicated by the introduction of two phenomena in the past two decades: the
digital computer and systems of distance learning, particularly over the Internet. The digital computer has
opened new possibilities in the laboratory, including simulation, automated data acquisition, remote control of
instruments, and rapid data analysis and presentation. Today, simulation software programs are available that
accurately emulate many technical and physical processes. These software programs play an important role in
engineering education (Quinn 1993).
Simulation is an important feature in engineering systems or any system that involves many processes. For
example in electrical engineering, delay lines may be used to simulate propagation delay and phase shift caused
by an actual transmission line (Kadlowec et al. 2002). Most engineering simulations entail mathematical
modeling and computer assisted investigation. There are many cases, however, where mathematical modeling is
not reliable. Simulation of such phenomena as fluid dynamics problems and materials processing often requires
both mathematical and physical simulations (Kadlowec et al. 2002).
In education, simulation has been used to provide illustrations of phenomena that are not easily visualized, such
as electromagnetic fields, laminar flow in pipes, heat transfer through materials, and electron flow in
semiconductors or solidification of liquid metal in a mold (Kadlowec et al. 2002). Since simulators essentially
execute mathematical equations and since we are able to develop reasonably accurate mathematical models of
the physical phenomena we study in engineering laboratories, it is natural that simulators have been used as an
adjunct to or even as a substitute for actual laboratory experiments. Simulations can be used as a pre-lab
experience to give students some idea of what they will encounter in an actual experiment (Hodge et al. 2001).
This can improve laboratory safety by familiarizing students with the equipment before actually using it. It also
can result in significant financial savings by reducing the time a student or team needs on real—and expensive—
laboratory equipment, thereby reducing the number of laboratory stations required. Simulations are useful for
experimental studies of systems that are too large, too expensive, or too dangerous for physical measurement by
undergraduate students (Baher 1999, Lee et al. 2001, Svajger and Valencic 2003).

361

�In this study, an example of use of simulation technique to demonstrate the effects of casting geometry on
solidification times of three different geometries in sand casting process been presented.

The Study
The objective of this study is to demonstrate how a simulation technique can be used instead of a real
casting laboratory (or a foundry) for technology students in engineering education. The objective is to deliver to
the students the effects casting geometries on the cooling and solidification time of liquid steel which allow
solidifying in sand molds.
The Theoretical Aspect of the Study The theoretical aspects of this study were taken from an engineering
textbook (Kalpakjian 1995) which deals with determining the solidification time of sand castings. During the
early stage of solidification, a thin solidified skin begins to form at the cool mold walls and, as time passes, the
skin thickens. With flat mold walls, this thickness is proportional to the square root of time. Thus doubling the
time will make the skin √2=1.41 times, or 41 times thicker. The solidification time is a function of the volume
(V) of a casting and its surface area (A), which is defined by Chvorinov’s rule as;

 V o lu m e 

Solidification time = C 
 S u rfaceA rea 

2

Eqn [1]

where C is a constant that reflects mold material, material properties (including latent heat) and temperature.
Thus a large sphere solidify and cools to ambient temperature at much slower rate than does a smaller sphere.
The reason is that the volume of a sphere is proportional to the cube of its diameter. Similarly, we can show that
the molten metal in a cube-shaped mold will solidify faster than in a spherical mold of the same volume.
Example: Three pieces being cast have the same volume (1dm3) but different shapes. One is a sphere, one a cube,
and the other a cylinder with a height equal to its diameter. We can determine which piece will solidify the
fastest and which one the slowest.
Solution: The volume is unity, so we have from Eqn 1;
1
Solidificatio time ∞ SurfaceAera
(
)

The respective surface areas are;
1

Sphere:

4
V =   π r 3 , r=  3  3 ,


3
 4π 

Cube:

V=a3,

2

 3 3
and A = 4 π r = 4 π 
 = 4 .8 4
 4π 
2

a = 1, and A = 6a2 = 6

1

2

 1  3 and A=2 πr 2 + 2 πr h = 6 πr 2 =  1  3 =5.54
Cylinder: V= πr h = 2 πr , r= 
6π 


 2π 
 2π 
2

3

Thus the respective solidification times t are;
Tsphere=0.043C, tcube=0.028C and tcylinder = 0.033 C. Hence the cube-shaped casting will solidify the fastest
and the sphere-shaped casting will solidify slowest.
Simulation: The above simple example of the effect of casting geometry on the solidification time of three
different shapes was computer simulated as follows. The given casting geometries were drawn as 3D solid
geometries using a CAD program. Each geometry was dimensioned to poses the required volume of 1 dm3 liquid
362

�metal. The solid geometries were then imported into a 3D casting simulation software. The simulation software
used in this study was able to create all the physical environment of a casting laboratuary or a foundry required
to solidify liquid alloy in sand mold of three different shapes. A steel alloy (ST1020) was chosen as the casting
alloy. Finally to solve required finite difference heat transfer equations the casting geometries were meshed with
a 40 mm thick sand mold for each into 1000 000 cubic element. The meshed casting geometries were cut into
two pieces from their common symmetrical planes to reduce the computation time as shown in Figure 1.

Figure 1: Isometric view of the meshed casting geometries used as models in simulation (as cut into half from symmetrical
planes) a-sphere, b-cylinder and c-cube.

Findings
Simulations were performed in computer environment. For the sake of simplicity and to model exactly
the same phenomena given in above example no running and feeding system was used in the casting model.
Figure 2 shows a scene of cooling and progress of solidification for each casting. As seen from Figure 1 advance
of solidification is fastest in cube-shaped casting and slowest in the sphere-shaped casting. This is in agreement
with the above given results from solution of the example. That is, the engineering students attending this class
will virtually see the solidification progress according to the theoretical rule and calculation without any real
experimental work in casting laboratory.
After the simulation have been completed post simulation evaluations were performed. Solidification
time of each casting is plotted to its quarter section of and shown in Figure 3. As seen from the figure the
solidification has lasted 14.66 minutes in sphere, which is the longest time as calculated in above example. This
is followed by cylinder and cube in exactly the same sequence as calculation. The smallest volume/surface area
value was calculated for the cube-shaped casting leading to a smallest solidification time. Results from the
simulation have also confirmed that the solidification has completed in 12.04 minutes in cube-shaped casting. It
is also in agreement with the results from mathematical calculation. This example illustrate that the casting
simulation technology can be useful to illustrate number of things which is difficult to or impossible when done
in a real laboratory environment. Thus, in such areas as castings where molten metals and high temperature are
often involved to experiment which might be dangerous, difficult and expensive to implement, laboratory can be
substituted by simulation.

363

�Figure 2: Cross-sectional view of progress of solidification in the cast parts a-sphere, b-cylinder and c-cube.

Figure 3: Plot of solidification times of the castings (qurter sections) a-sphere, b-cylinder and c-cube.

Conclusions
In this study, an example of use of simulation technique to demonstrate the effects of casting geometry
on solidification times of three different geometries in sand casting process been presented.
Theoretical background and an example were given to calculate the solidification times of three
different geometries as sphere, cube and a cylinder. Result from calculation showed that cube-shaped casting
will solidify the fastest and the sphere-shaped the slowest.
Computer simulation of the solidification process with 3D CAD data of the geometries was also
performed. Results from simulation were in excellent agreement with the result from calculations. This
confirmed that casting simulation technology might substitute the casting laboratory in engineering education.
This can be even more useful for such experiments where high temperature and expensive experimental set-up
involves.

Acknowledgement
Author thanks to Sakarya University -Turkey for financial support through BAPK. (Project No: 2007-05-06-004).

364

�References
Baher, J., “Articulate Virtual Labs in Thermodynamics Education: A Multiple Case Study,” Journal of
Engineering Education, Vol. 88, No. 4, 1999, pp. 429–434.
Hodge, H., Hinton, H.S., and Lightner, M., “Virtual Circuit Laboratory,” Journal of Engineering Education, Vol. 90, No. 4,
2001, pp. 507–511.
Kadlowec,J., Lockette, P.V., Constans, E., Sukumaran, B., and Cleary, D., “Visual Beams: Tools for Statics and Solid
Mechanics,” 32nd ASEE/IEEE Forntiers in Education Conference, Boston Mass., November 6–9, 2002, pp. T4D-7-T4D-10,
2002.
Lee, W-J., Gu, J-C., Li, R-J., and Ditasayabutra, P., “A Physical Laboratory for Protective Relay Education,” IEEE
Transactions on Education, Vol. 45, No. 2, 2002, pp. 182–186.
Quinn, R. "The E' Introductory Engineering Test, Design and Simulation Laboratory" Journal of Engineering Education, Vol.
82, No. 4, October 1993.
S.Kalpakjian, Manufacturing Engineering and Technology, 3rd Edtn.,Addison-Wesley N.York, 1995.
Svajger, J., and Valencic, V., “Discovering Electricity by Computer- Based Experiments,” IEEE Transactions on Education,
Vol. 46, No. 4, 2003, pp. 502–507.
Wankat, P.C., “Analysis of the First Ten Years of the Journal of Engineering Education,” Journal of Engineering Education,
Vol. 93, No. 1, 2004, pp. 13–21.

365

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                <text>Kayıkcı, Ramazan</text>
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                <text>The function of the engineering profession is to manipulate materials, energy, and  information, in that way creating benefit for humankind. To perform this effectively, engineers  must have knowledge of nature that goes beyond plain theory that is traditionally gained in  educational laboratories. In recent years, however, the environment of these laboratories has  changed. This paper describes the use of simulation technology in engineering education. In  particular, the paper considers materials and metallurgical engineering field which involves  many high temperature materials processing such as castings and solidification. In this study,  an example of use of simulation technique to demonstrate the effects of casting geometry on  solidification times of three different geometries in sand casting process been presented.</text>
              </elementText>
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                    <text>2nd International Symposium on Sustainable Development, June 8-9, 2010 Sarajevo

Sustainability and Feasibility of English-medium Instruction at Turkish
Universities
Asst. Prof. M. Naci Kayaoğlu
Faculty of Letters
Karadeniz Technical University
Turkey
naci@ktu.edu.tr
Emine Buket Sağlam
School of Foreign Languages
Karadeniz Technical University
Turkey
saglambuket@hotmail.com

Abstract: More and more often, universities in Turkey are adopting English as a
medium of instruction. In some cases English is the medium for entire faculties while
in others it is only used in certain departments. One notable signal of this
development is the number of universities offering compulsory one year English prep
programs that take place before the student matriculates in to their own department or
faculty. More than 110 Turkish universities have turned to English as a medium of
instruction to varying degrees, investing a huge amount of capital and human
resources. While we recognize that learning to speak and write in English in this age
of globalization is of great help and necessity in order to be able to compete in a
knowledge-based world, the current situation is not without grave concerns. In many
ways it runs counter to the effectiveness and quality of higher Education. Based on a
cased study, this paper examines sustainability and feasibility of English medium of
instruction.

Introduction
One of the most significant projects carried out by Karadeniz Technical University over the last ten
years is the regulation of and the investment in foreign languages (mainly English). That the fourteen
departments including the Medical school now have compulsory English prep program for a year and also 30 %
of the vocational courses at the departments will be in English indicates that English teaching is a serious
endeavor at Karadeniz Technical University. It also indicates that the university hopes the use of English in all
the fourteen departments might lead to more effective results on education and training programs. However, it
should be noted that when the data was collected there were fourteen departments which had one-year obligatory
English prep school. Today, the number of the programs which has one-year obligatory English prep program
has reached 24.
Now that we have a one year compulsory English prep program with many students and English
instructors, the time has come to evaluate the program by examining it from many academic and scientific
approaches in hopes of becoming part of the European Union Education programs. Our aim in this examination
is to ease the processes of accreditation and free moving system in Europe. It is obligatory to determine the
strategies in foreign language teaching and learning under the content of The European Languages Portfolio.
Additionally, the implementation of The Language Passport throughout Europe is a motivating factor for
reviewing all the foreign language teaching and learning issues in the institution. The policies and the strategies
of the Foreign Language should be reconstructed under the light of the scientific data and strong theories which
have been offered by the existing literature (Christison &amp; Stoller, 1997).
The purpose of this study is therefore to determine the problems and the difficulties in teaching the
vocational courses in English at the university level, from the points of the view of the students and the academic
staff. Within this framework, several other goals are brought to light as well: (1) To increase the quality and the
productivity of the obligatory English prep program, (2) To determine the strategies which will contribute to
solve the problems faced during the educational process in which the courses are taught in English in the
departments.

195

�2nd International Symposium on Sustainable Development, June 8-9, 2010 Sarajevo

Method
This study is a case study which reveals the problems and the difficulties in teaching the vocational
courses in English in the fourteen departments at a Turkish university from the points of view of the students and
the academic staff. The sample of this study consists of 1442 students and 52 academic staff from various
departments at Karadeniz Technical University. A questionnaire for the academic staff including four openended questions has been used for data collection. Questionnaire used for the students who studied one year at
prep had 20 items in order to evaluate the sustainability and productivity of the English medium in vocational
courses. This questionnaire was given to the students studying in their second, third and fourth year of their
training. The data from this questionnaire was later compared to those which were obtained from the
questionnaire given to English prep school students.

Findings
The data were obtained from three samplings: (1) from the students at English prep school, (2) from the
students who studied one year at prep, (3) from the academic staff who teaches their courses in English at the
departments.
1. The data obtained from the students at English prep school: According to Table 1, the participants
are composed of the students from 14 departments at Karadeniz Technical University. 4,5% is from Computer
Engineering, 5,6% is from Biology, 8,9% is from Electric-Electronic Engineering, 5,3% is from Physics, 4,1% is
from the Deck Department, 8,8% is from Civil Engineering, 8,1% is from Geodesy, 6,0% is from Geology,
6,5% is from Public Administration, 7,3% is from Chemistry, 11,4% is from Mechanical Engineering, 9,6% is
from Forest Engineering, 7,6% is from the Medical Faculty, and 4,7% is from the International Relations.
Departments

N

%

Computer Engineering

36

4,5

Biology

45

5,6

Electric-Electronic

72

8,9

Physics

43

5,3

Deck

33

4,1

Civil Engineering

71

8,8

Geodesy

65

8,1

Geology

48

6,0

Public Administration

52

6,5

Chemistry

59

7,3

Mechanical Engineering

92

11,4

Forest Engineering

77

9,6

Medical Faculty

61

7,6

International Relations

38

4,7

Table 1. The Number of the Departments and the Students Participated in the Study

196

�2nd International Symposium on Sustainable Development, June 8-9, 2010 Sarajevo
Table 2 shows the evaluations of the participants for the speaking courses. According to the data
obtained from the questionnaire, 23.3 % of the students state that at prep school English instructors are not wellprepared for the lessons whereas 19.5 % of them have no idea. However, 56.6 % of the students agree that the
instructors are well-prepared. For the second item, 53.1% of the students think that the instructors are punctual
whereas 21.6% disagree. 65% of the students agree that the teachers explain lessons well. However, 21.6
disagree. For the fourth item, 69.1% of the students think that the instructors encourage every student to
participate in the lesson. 72.4 of the participants consider that their teachers respect their personality. As for the
sixth item, 76.9% of the participants consider the instructors encourage them to speak in English in the class.
68.8% of them think that the instructors tolerate their mistakes. 50.4% of the participants consider the instructors
encourage them to join the lesson. 58.1% of them think that the instructors support them during the preparation
of their projects. As for the projects prepared during the academic year, 73.4% of the participants think that the
projects in the first term are useful. 55.9% of the students consider the projects in the second term are beneficial.
For 67.2% of the participants, the materials are beneficial.

ITEMS
INSTRUCTORS;

1. are well-prepared for the lessons
2. are always punctual
3. explain lessons well
4. encourage every student to
participate the lesson
5. respect our personality
6. encourage us to speak in English
in the class
7. tolerate our mistakes
8. encourage us to join the lesson
9. support us during the preparation
of our projects
Items
Instructors;
10. given projects (first term)
11. given projects (second term)
12. Materials used

STRONGL
Y
DISAGRE
E
12,8
14,3
11,7
7,8

DISAGRE
E

NO
IDEA

AGRE
E

STRONG
LY
AGREE

MISSI
NG

10,5
13,9
9,9
6,5

19,5
17,2
12,4
15,5

31,9
25,4
30,8
38,6

24,7
27,7
34,2
30,5

1,6
1,5
1,0
1,3

6,6
6,7

3,7
5,3

15,3
8,9

31,1
28,3

41,3
48,6

2,0
2,3

7,6
19,9
14,0

5,3
11,2
10,3

17,4
17,7
16,0

30,6
21,6
23,7

38,2
28,8
34,4

0,9
0,8
1,6

quite
useless
8,6
17,7
12,7

useless

normal

useful

Missing

4,8
8,2
6,6

11,0
16,4
12,7

28,2
20,8
26,1

very
useful
45,2
35,4
41,1

2,1
1,5
1,0

Table 2. The data obtained from the Speaking courses
Table 3 illustrates the evaluations of the participants for the reading courses. According to Table 3, 23.5
% of the students state that at prep school English instructors for the reading courses are not well-prepared for
the lessons whereas 20.2 % of them have no idea. However, 55.8 % of the students agree that the instructors are
well-prepared. For the second item, 52.6% of the students think that the instructors are punctual whereas 26.6%
disagree. 67.6% of the students agree that the teachers explain lessons well. However, 16.5 disagree. For the
fourth item, 67.5% of the students think that the instructors encourage every student to participate in the lesson.
75.8 of the participants consider that their teachers respect their personality. As for the sixth item, 81.5% of the
participants consider the instructors encourage them to speak in English in the class. 70.8% of them think that
the instructors tolerate their mistakes. 51.4% of the participants consider the instructors encourage them to join
the lesson. 53.1% of them think that the instructors support them during the preparation of their projects. As for
the projects prepared during the academic year for the reading courses, 71.6% of the participants think that the
projects in the first term are useful. 62.8% of the students consider the projects in the second term are beneficial.
For 71.9% of the participants, the materials are beneficial.

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ITEMS
INSTRUCTORS;

1. are well-prepared for the lessons
2. are always punctual
3. explain lessons well
4. encourage every student to participate the lesson
5. respect our personality
6. encourage us to speak in English in the class
7. tolerate our mistakes
8. encourage us to join the lesson
9. support us during the preparation of our projects
Items
10. given projects (first term)
11. given projects (second term)
12. Materials used

STRONG
LY
DISAGR
EE
10,9
12,2
8,1
5,8
4,0
5,3
5,6
13,8
13,2
quite
useless
5,5
9,1
8,6

DISAGR
EE

NO
IDEA

AGR
EE

STRONG MISSIN
LY
G
AGREE

12,6
14,4
8,4
10,0
4,0
4,4
7,0
12,0
13,0
useless

20,2
19,7
14,9
15,7
13,2
6,4
15,6
21,5
19,8
normal

29,8
23,5
29,5
37,1
30,3
28,8
31,8
22,8
25,3
useful

6,0
9,1
6,0

15,6
17,6
12,5

32,3
26,4
27,5

26,0
29,1
38,1
30,4
45,5
52,7
39,0
28,6
27,8
very
useful
39,3
36,4
44,4

0,5
1,1
1,0
0,8
3,0
2,3
1,0
1,3
0,8
Missing
1,3
1,4
1,0

Table 3. The data obtained from the Reading courses
Table 4 illustrates the evaluations of the participants for the grammar courses. According to Table 4,
64.7 % of the students state that at prep school English instructors for the grammar courses are well-prepared for
the lessons whereas 17.5 % of them have no idea. For the second item, 69.5% of the students think that the
instructors are punctual. 80.5% of the students agree that the teachers explain lessons well. However, 16.5
disagree. For the fourth item, 59.2% of the students think that the instructors encourage every student to
participate in the lesson. 57.9 of the participants consider that their teachers respect their personality. As for the
sixth item, 86.6% of the participants consider the instructors encourage them to speak in English in the class.
71.1% of them think that the instructors tolerate their mistakes. 66.8% of the participants consider the instructors
encourage them to join the lesson. 63.8% of them think that the instructors support them during the preparation
of their projects. As for the projects prepared during the academic year for the reading courses, 79.5% of the
participants think that the projects in the first term are useful. 76.9% of the students consider the projects in the
second term are beneficial. For 80.5% of the participants, the materials are beneficial.
ITEMS
INSTRUCTORS;

STRON
GLY
DISAG
REE

DISAGR
EE

NO
IDEA

AGR
EE

STRON
GLY
AGREE

MISSING

1. are well-prepared for the lessons
2. are always punctual
3. explain lessons well
4. encourage every student to participate the
lesson
5. respect our personality
6. encourage us to speak in English in the
class
7. tolerate our mistakes
8. encourage us to join the lesson
9. support us during the preparation of our
projects

7,3
6,8
4,5
13,2

9,3
7,7
4,7
11,0

17,5
143,7
8,8
15,6

29,2
28,7
28,7
30,4

35,5
40,8
51,8
28,8

1,1
1,3
1,3
1,0

3,8
2,1

2,9
1,7

13,0
7,4

28,5
29,5

29,4
57,1

2,4
97,8

6,8
6,8
6,3

6,7
6,0
9,3

14,4
19,1
19,8

29,9
25,9
28,8

41,2
40,9
35,0

1,1
1,3
0,1

Items

quite
useless

useless

normal

useful

very
useful

Missing

10. given projects (first term)
11. given projects (second term)
12. Materials used

3,6
4,5
4,6

3,1
5,5
2,9

12,1
11,7
10,8

32,1
25,0
29,0

47,4
51,9
51,5

1,5
1,6
1,3

Table 4. The data obtained from the Grammar courses

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�2nd International Symposium on Sustainable Development, June 8-9, 2010 Sarajevo

Table 5 illustrates the evaluations of the participants for the writing courses. According to Table 5, 32.7
% of the students state that at prep school English instructors for the writing courses are well-prepared for the
lessons whereas 20.6 % of them have no idea. However, 35.9 % of the students agree that the instructors are not
well-prepared. For the second item, 46.8% of the students think that the instructors are punctual whereas 33.7%
disagree. 61.3% of the students agree that the teachers explain lessons well. For the fourth item, 59.1% of the
students think that the instructors encourage every student to participate in the lesson. 65.6 of the participants
consider that their teachers respect their personality. As for the sixth item, 87.3% of the participants consider the
instructors encourage them to speak in English in the class. 60.9% of them think that the instructors tolerate their
mistakes. 45.3% of the participants consider the instructors encourage them to join the lesson. 44.7% of them
think that the instructors support them during the preparation of their projects. As for the projects prepared
during the academic year for the reading courses, 63.3% of the participants think that the projects in the first
term are useful. 68.1% of the students consider the projects in the second term are beneficial. For 68.6% of the
participants, the materials are beneficial.
ITEMS
INSTRUCTORS;

1. are well-prepared for the lessons
2. are always punctual
3. explain lessons well
4. encourage every student to participate the lesson
5. respect our personality
6. encourage us to speak in English in the class
7. tolerate our mistakes
8. encourage us to join the lesson
9. support us during the preparation of our projects
Items
10. given projects (first term)
11. given projects (second term)
12. Materials used

STRON
GLY
DISAG
REE
20,8
19,4
13,1
9,9
7,5
4,2
8,7
18,2
19,4
quite
useless
9,0
14,0
12,0

DISAG
REE

NO
IDEA

AGR
EE

STRON
GLY
AGREE

MISS
ING

15,1
14,3
9,2
9,3
5,3
5,6
8,9
11,1
14,0

20,6
18,0
15,5
19,4
18,9
9,9
20,1
24,0
20,7

23,4
23,5
27,0
34,5
26,4
29,9
28,2
20,7
23,6

19,3
23,3
34,3
25,6
39,2
48,4
32,7
24,6
21,1

0,8
1,5
0,9
1,3
2,7
2,0
1,4
1,4
1,1

useless

normal

useful

9,3
8,2
4,9

16,6
17,9
13,5

28,1
22,8
27,3

very
useful
35,2
35,3
41,3

Missi
ng
1,7
1,7
1,1

Table 5. The data obtained from the Writing courses
The data obtained from the students who studied one year at prep:The following are the tables
illustrating the results of the study conducted at Karadeniz Technical University in 2008-2009 academic years.
Table 6 shows which departments participated in the study. Table 7 informs us about the age of the students who
participated in the study.

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NAVAL
ARCHITECT
URE
F 36
% 5,9

MARITIME
TRANSPORTA
TION

DEC
K

123
20,1

5
0,8

ELECRICA
LELECTRO
NIC
129
21,0

CIVIL
ENGINEERI
NG
125
20,4

GEOMATIC
S
ENGINEERI
NG
33
5,4

MECHANIC
AL
ENGINEERI
NG
162
26,4

Table 6. The student frequencies by percentages according to the departments

Table 7: Age Percentages
In Table 8, 72,9% of the students agreed that English prep school is necessary for them. 85,1% stated
they have vocational courses in English at their department. 82,6% said not all the courses should in English at
their department. 37,4% circled “yes” for item 4, which is “Are at least 2 courses in English at your
department?”. For item 5, which is whether the vocational courses should be completely in English, only 54,5%
said “yes”. 52,6% of the students think it is advantageous to have their courses related to your branch in English
in their work life in the future. 20,6% state that the instructors speak in English all through the lesson. 69,8%
state they do not have enough reference books suitable for the vocational courses in English. After the English
prep school, 65,7% think their English level has shown regression.

ITEMS
Q1. Is prep school necessary for your department?
Q2. Are you having vocational courses in English at your department?
Q3. Should all the courses be in English at your department?
Q4. Should at least 2 courses be in English at your department?
Q5. Should the vocational courses be completely in English?
AGE
F
%

18
4
0,7

19
36
6,0

20
100
16,5

21
177
29,3

22
145
24,0

23
77
12,7

YES (%)
72,9
85,1
17,3
37,4
54,5
24
48
7,9

Q6. Do you think it is advantageous to have your courses related to your
branch in English in your work life in the future?
Q7. Does the instructor speak in English all through the lesson?
Q8. Do you have enough reference books suitable for the vocational courses in
English?
Q9. After the prep school, do you think your English level has shown
regression?

25
13
2,1

26
4
0,7

NO (%)
27,1
14,9
82,6
62,6
45,5
27
1
0,2

52,6

47,4

20,6
30,2

79,4
69,8

65,7

34,4

Table 8. The student questionnaire for the items 1-9 according to “yes” “no” percentages
According to the results of the questionnaire, 36,8% of the participants state they are sometimes given
homework in English whereas 36,7% claim that they are never. While 10,2% state that they are always using
English books as references for their courses, 38,6 say they never. 30,3% find the instructors are never sufficient
in English whereas 25,7% say they sometimes are. 25,8% always find the courses in English are comprehensible
while 17,7% think they never are. The results of the items 10, 11, 12, and 13 are illustrated in Table 9.

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ITEMS

NEVER

SOMETI
MES

OFTEN

USUAL
LY

ALWAY
S

Q10. Are you being given homework in English related
to your field?
Q11. Are you using English books as references for
your courses?
Q12. Do you think the instructors are sufficient in
English while teaching?
Q13. Do you have difficulty in comprehending the
courses in English?

36,7

36,8

15,6

7,1

3,8

38,6

24,4

15,6

11,3

10,2

30,3

25,7

20,9

14,0

9,0

17,7

18,4

19,3

18,7

25,8

Table 9. The student questionnaire for the items 10-13 according to adverbs of frequency percentages
Table 10 contains the questionnaire items 14, 15, and 16. As for item 14, which is “What is the
percentage of the talk in English in the lessons done by the instructors?”, 32.5% of the students think that 7090% of the talk is in English whereas 12,3% state 10-30% of the talk is in English. For item 15, which is “What
is the percentage of the talk in English in the lessons done by the students?” 2,2% of the students think that 7090% of the talk is in English whereas 75,5% state 10-30% of the talk done by the students is in English
ITEMS

10-30%

30-50%

50-70%

70-90%

Q14. What is the percentage of the talk in English in
the lessons done by the instructors?
Q15. What is the percentage of the talk in English in
the lessons done by the students?
Q16. What is the percentage of the talk in English in
the lessons?

12,3

9,8

15,7

32,5

90100%
29,8

75,5

15,7

6,1

2,2

0,5

20,8

23,4

27,9

23,2

4,6

Table 10. The student questionnaire for the items 14-16 according to the scale of percentages
In Table 11, the answer to the question “What is the productivity of the lessons in English?” is “normal”
for the 33,2% of the students, “productive” for the 8.9% of the students, and “poor” for the 57.9% of the
students.
ITEMS
VERY
PRODUCT NORMA POOR
VER
PRODUCT IVE
L
Y
IVE
POO
R
Q17. What is the productivity of the lessons
1,3
7,6
33,2
32,7
25,2
in English?
Table 11. The student questionnaire for the item 17
The data obtained from the academic staffs who teach their courses in English at the departments.
Table 12 contains the questionnaire items 1, 2, 3, and 4, which is given to the instructors who teach
their courses (at the department) in English. According to the table, 92,2% of the instructors agree that prep
school is necessary for their department. 74,5% agree that there are enough reference books in the library related
to their department. 40,8% agree it is necessary to teach the courses in English at the departments. 80% state that
there are enough materials to use in the courses in English.
ITEMS
Q1. Is prep school necessary for your department?
Q2. Are there enough reference books in the library related to your
department?
Q3. Is it necessary to teach the courses in English?
Q4. Do you lack materials in the courses while teaching?

201

YES
92,2
74,5

NO
7,8
25,5

40,8
20

59,2
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�2nd International Symposium on Sustainable Development, June 8-9, 2010 Sarajevo

Table 12. The instructor questionnaire for items 1-4 according to “yes” “no” percentages
According to Table 13, for item 5, 62% of the instructors state that their students are “sometimes” able
to use their English efficiently in the courses at their department. 29,4% “always” suggest their students they use
reference books in English in their field courses. 43,1% state that their students are sometimes able to prepare
their papers in English whereas 27,5% think the students never can. Additionally, 41,2% think that their students
are “sometimes” able to understand what they read in English in their field.
ITEMS

NEVER
8

SOMETIM OFTEN
ES
62
14

USUAL
LY
12

ALWA
YS
4

Q5. After studying English at Prep school, are your
students able to use their English efficiently in the
courses at your department?
Q6. Do you suggest your students they use reference
books in English in their field courses?
Q7. Are your students able to prepare their papers in
English?
Q8. Are your students able to understand what they
read in English in their field?

4

17,6

23,5

25,5

29,4

27,5

43,1

17,6

7,8

3,9

23,5

41,2

19,6

13,7

2

Table 13. The instructor questionnaire for the items 10-13 according to adverbs of frequency percentages
According to Table 14, the instructors have 47.1% difficulty in Speaking during the lessons. This is
followed by Writing and Reading.
Q9. WHICH SKILLS BOTHER YOU
TEACHING TO YOUR STUDENTS?
Reading, Speaking, Writing
Reading, Speaking
Writing
Writing, Speaking
Speaking
None of them

MOST

WHILE

F

%

7

13.7

1
4
8
24
5
Table 14. The instructor questionnaire for the item 9

2.0
7.8
15.7
47.1
9.8

As for the evaluation of the productivity of the courses in English, 50% find the courses in English
“normal” and “productive” whereas 50% find them “poor” and “very poor”. As for the “teachability” of the
courses scheduled in English at Karadeniz Technical University, 50% find them “normal” and 33,3% find them
“bad”. These findings are illustrated in Table 15.
ITEMS
Q10. How do you evaluate the productivity of the courses
taught in English?
Items
Q11. How do you evaluate the “teachability” of the courses
scheduled in English at our university?

PRODUC
TIVE
6

NORM PO
AL
OR
44
46

VERY
POOR
4

Good

Normal

Bad

Very bad

14,6

50,0

33,3

2,1

Table 15. The instructor questionnaire for the items 10-11

Discussion
This study sought to better understand how feasible and effective to adopt English as a medium of
instruction in the departments or faculties at Karadeniz Technical University after having one-year English prep

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�2nd International Symposium on Sustainable Development, June 8-9, 2010 Sarajevo
program. Thus, it would be easier to determine more effective and productive foreign language policy, mainly
English. With the help of this policy, the university would add a global etiquette to its local identity.
The analysis of the descriptive statistics showed that great amount of the students and teachers consider
English prep school is necessary for their departments. This finding indicates that prep school is a necessity at
the departments included in the study. However, for using English as a medium of instruction, 60% of the
teachers showed objection. Likewise, 82.5% of the students disagreed that in all vocational courses English
should be used as a medium of instruction. Similarly, 62.6% of the participants’ (students) answer was “no” to
the question “Should at least 2 courses be in English at your department?”. These findings might be interpreted
as both instructors and the students do not want English to be used as a medium of instruction. 52.6% of the
students consider it is advantageous to have their courses related to their branch in English in their work life in
the future. This might indicate that although it is not in a high percentage, many students consider that having
courses in English can be advantageous in their future life. However, it has been observed that there is a
controversy between this finding and the students’ responses to the questions “Should at least 2 courses be in
English at your department?”, “Should all the courses be in English at your department?”, and “Should at least 2
courses be in English at your department?” since 62.6% and 82.5% of the students said “no”. “Does the
instructor speak in English all through the lesson?” is another question which was responded with a “no” by 80%
of the students. This can be explained by a response which was given to the question whether their English level
has shown regression during their four-year education. That is 65.7% of the students responded this question as
“yes”. From this finding, it might be interpreted that these students might show a regression in their foreign
language knowledge. This can be supported by the data that 43.2% of the instructors consider their students can
“sometimes” prepare their papers in English. Additionally, only 41.2% of the instructors think that the students
might comprehend what they read in their major in English. As for the reference books in English, although 80%
of the instructors state that they have enough of them, 70% of the students state that they lack reference books at
their departments. This finding can be interpreted as the instructors show not enough effort and guidance for the
students to reach these sources.
The findings from the data also show that instructors do not force their students to use their knowledge
in English. This can be supported by the answer “sometimes” of 62% of the instructors to the question whether
the students use English after prep school in their major. The difficulty that the instructors have in the courses is
mostly in verbal expressions, in other words speaking (47.1%). This finding can be explained by the respond
which the instructors gave to one of the open-ended questions. The instructors claim that the students cannot
comprehend what they read and listen. Besides the instructors add that the students cannot efficiently use their
prep English after they have started to study their major. Both the students and the instructors do not specifically
wish their courses to be English. The reason for this might be the perspective of the teachers and the students
who do not favor the use of English in their major. 59% of the instructors are not in favor of using English as a
medium of instruction in their courses. This might directly be affecting the view students have.
As a result, it is known that in the courses with the students who consider their English has shown
regression after prep and who cannot reach reference books in English, which has a salient role in their studies,
46% of the instructors evaluate the feasibility and the productivity as inefficient. At the university base, 33% of
the instructors graded it as “bad”, and 50% graded it as “normal”. As for the evaluation of the students about the
feasibility and productivity of the courses in English, 33% find the courses ineffective.
Giving the importance to the quality of education and having universal values, Karadeniz Technical
University has recently restructured its foreign language policies and priorities. It should be noted that today 24
departments have one-year English prep school. In order to maintain the success of an English prep program for
the 24 departments, the administration of KTÜ has offered 30% of the courses in English. Some of the
postgraduate courses at the university are also using English as a medium of instruction. The students who
studied English prep for a year were previously claimed that they were not able to utilize the language efficiently
after they had matriculated in to their own faculty or departments. The reasons for that might be the lack of
motivation of these students. Most of them do not internalize their major and 47.4% of them consider learning
English is advantageous. Also, the lack of sustainability of using English as a medium of instruction can be
explained by the views of the instructors. That is, although 92% of the instructors agree that there should be a
prep program for their departments, 59% consider that using English as a medium of instruction is unnecessary.
The study can be summarized in a chart as follows:

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�2nd International Symposium on Sustainable Development, June 8-9, 2010 Sarajevo
The Administrative Policy at KTÜ
Having 30% of the courses in English
The number of the departments which has English
prep program increased from 14 to 24
Materials used
Writing S. (82%)
Reading S. (84%)
Speaking S. (80%)
Grammar S. (91%)

The Feasibilty and the Sustainability
of English as a Medium of
Instruction at University Level

Reference Books
Instructor (80%)
S. (30%)

(+)

(+)
English Prep School

(+)

Field Courses in English

(-)

(+)

(-)
(-)

Ideas
Instructor (92.2%)
S. (%73%)

Ideas
Instructor(%18)
S. (27%)

Ideas
Instructor (41%)
S. (17%)

Ideas
Instructor(59%)
S. (83%)

Productivity
Instructor (50%) (-/+)
S. (58%) (-)
According to the chart, most of the instructors have negative ideas about teaching the field courses in
English. This is thought to affect feasibility and the productivity of the courses in English. Likewise 83% of
the students disagree that the courses should be in English. Also, the students in the survey consider they
lack enough materials in English in their field whereas 80% of the instructors state that there are enough of
materials. Additionally, there is a controversy between the ideas of both students and teachers about
studying English prep and using English in their field courses. At the prep level, the majority of the students
and the instructors agree that there should be an English prep program for their departments, both disagree
in continuing studying their major in English. The lack of motivation, the idea that it is not necessary to
learn English in their major, and lack of prediction that they might need English in their future life might be
some of the reasons which affect the feasibility and the sustainability of using English at a university level
as a medium of instruction.

Conclusion
All in all, considering the priorities and the foreign language policies of the administrative staff at KTÜ,
it is possible to say that the administration gives importance to foreign language teaching at KTÜ, mainly
English. However, it should be noted that for a better applicability, feasibility, and sustainability of using English
as a medium of instruction, human sources, realities of the departments, conscious of the students about learning
a foreign language, and the perspectives of the academic staff should be taken into consideration.

Reference
Christison, M.A., Stoller, F. L. (1997). A Handbook for Language Program Administrators, Burlingame, CA: Alta Book
Center.

204

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                <text>More and more often, universities in Turkey are adopting English as a  medium of instruction. In some cases English is the medium for entire faculties while  in others it is only used in certain departments. One notable signal of this  development is the number of universities offering compulsory one year English prep  programs that take place before the student matriculates in to their own department or  faculty. More than 110 Turkish universities have turned to English as a medium of  instruction to varying degrees, investing a huge amount of capital and human  resources. While we recognize that learning to speak and write in English in this age  of globalization is of great help and necessity in order to be able to compete in a  knowledge-based world, the current situation is not without grave concerns. In many  ways it runs counter to the effectiveness and quality of higher Education. Based on a  cased study, this paper examines sustainability and feasibility of English medium of  instruction.</text>
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                    <text>2nd International Symposium on Sustainable Development, June 8-9, 2010 Sarajevo

An Education Structuring Model in Vocational and Technical Education
with The Orijins of Young Lecturers: Kaman Vocational School
Prof.Dr. Ali Đhsan Karayiğit
Ahi Evran University, Kaman Vocational School, Kaman, Kırşehir/Turkey
aikarayigit@ahievran.edu.tr
Assist.Prof.Dr. Latif Onur Uğur
Ahi Evran University, Kaman Vocational School, Kaman, Kırşehir/Turkey
lougur@ahievran.edu.tr

Abstract: In this study, it is discussed that a breakthrough approach to education with young
faculty members in vocational technical education. Kaman Vocational School which was
connected another university before, connected Kırşehir Ahi Evran University with a decision
received in its foundation date in 2006. Continuing its tarining activities given 160 students three
departments, two permanent faculty members and the others coming from nearby provinces;
Kaman Vocational School has gained acceleration with a department at four years, students over
1700 and 48 faculty members and the radical decisions given by university management. Not
only physical growth but while improving the quality of education with seminars, conferences,
technical tours, modern laboratories, important scientific progress achieved by numerous
scientific articles and reports, papers, original and alternative textbooks. Under European Union
(EU) projects, it was participated with lots of faculty members and students to eight different
countries and projects; the social integration increased with the EU higher education and youth
arrangements. Successes in sporting activities held within the Vocational School and the
university; higher artistic levels in light music, folk music, rock music concerts, theatres, musical
and poetry nights prepared by student groups; publishing students’ works that take a degree and
honorable mention in story and poetry writing competitions are all provided with the
contributions of young and dynamic academic staff. Among over 550 Vocational School in
Turkey, Kaman Vocational School that catches on the tops in a short time, constituted a sample
training model. In the study, in this model and the role of young teaching staff and the added
value they provide within this model is discussed with the help of related data.
Key Words: Vocational and Technical Education, Vocational School, Education Model,
Education Technology, Human Resources, Young Faculty Members.

Introduction
Kırşehir Faculty of Education which is considered to be the beginning of Ahi Evran University was
opened as Male Teacher’s Training School in 1961 and was transformed into an Institution of Education in
1974-1975 academic -year. In 1982, it was transformed into a (two- year) College of Education and became
affiliated to the Gazi Faculty of Eduction of the University of Gazi. In 1988 , the education period was increased
to four years and in 1992 it became the Kırşehir Faculty of Education in accordance with the Law numbered
3837.
Kaman Vocational School of Higher Education (Kaman MYO) started its education life within the
body of University of Hacettepe as per the decree of General Staff dated 23rd January, 1998 and numbered 8 and
was affiliated to the University of Gazi in accordance with the protocol signed between University of Gazi and
University of Hacettepe as per the decree of General Staff dated 4th April, 2003 and numbered 39. Kaman MYO
was later affiliated to the newly established University of Ahi Evran as per the law dated 1st March, 2006 and
numbered 5467. There are still 5 Faculties, 3 Colleges, 4 Vocational Schools of Higher Education, and 3
Institutes.
University of Ahi Evran still continues to its activities with its experienced staff who are devoted to the
principles and revolutions of Ataturk, with its current physical conditions and with the excitement of being a
newly established university.
In 1913 Kaman became a township centre and had a municipality. It became a district on September 1,
1944. It became affiliated to Ankara in 1954 when Kırşehir became a district itself. It again became affiliated to

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Kırşehir after three years when Kırşehir received the status of a province again. According to the archeological
excavations , Kaman and its round has been a settlement place since B.C. 3000.
Industry has been also improving in Kaman whose main source of living is agriculture and husbandry .
The three local papers in Kaman (Kaman Akhaber, Yesil Kaman and Kaman Postası) are weekly issued. A
Cultural Centre Building with its well-equipped, 500 people capacity conference hall and a Public Library with
lots of books and reading halls are present for the service of all level-students and guests.
Although the population of Kaman is 22,000 people, the level of education is very high and it has a very
high schooling level which is even much higher than the average in Turkey. The high schools which are
generally the main source of the students of Kaman MYO and which are located in the town centre are Kaman
Anatolian High School , Kaman Anatolian Teacher’s Training High School, Kaman Anatolian Trade Vocational
High School , Imam Hatip High School, Vocational School for Girls, Kaman High School and Kaman Medicine
Vocational High School . Thanks to the quality of its education, Kaman is rapidly improving to be a training
base and the role of Kaman MYO in this is crucial.
The historical Kalehoyuk, Archaeology Museum, Japon Botanic Garden, Hirfanlı Dam and HEP,
Ostrich Farm, Closed sports centre, Atletism Stadium, Swimming Pool, Water Sports Centre, mausoleum of the
great minstrel Dadaloglu, Walnut tree gardens are some of the touristy, cultural and sporty facilities in Kaman or
its round.
Kaman MYO started giving education within the body of University of Hacettepe in the academic year
of 1998-1999 . The programmes of Food Technology, Construction, Marble Processing and Drilling were
opened within the Department of Technical Programmes during the period of foundation. Furthermore, new
students have been accepted to the Department of Computer Technologies and Programming within the scope of
Ministry of National Education (MEB) - Higher Education Council (YOK) Project of Improving the Vocational
Schools of Higher Education since 2002-2003. The school continued its academic life by being transferred into
the body of University of Gazi in accordance with the protocol signed between University of Gazi and
University of Hacettepe in 2003. Halls of residence with a high capacity which can meet the accommodation
needs of both female and male students have been built in Kaman by Credit and Dormitories Institution
(YURTKUR) and the facilities of food, studies, internet connection and cafeterias are available in these
residence halls. After graduation, it is possible for the students to be transferred to the faculties of four-year
education through the External Transfer Exam. The course language is Turkish .
In the academic year which will start in September, 2010, the departments at Kaman MYO and their
quotas are listed in Table 1.
Programmes
Computer Programming
Computer Programming (E . E . )
Office Services and Executive Assistance
Office Services and Executive Assistance (E . E . )
Technology of Natural Building Stones
Electricity
Electricity (E . E . )
Electronic Correspondence Technologies
Electronic Correspondence Technologies (E . E . )
Technology of Electronics
Food Technology
Food Technology (E . E . )
Mapping and Cadastre
Mapping and Cadastre (E . E . )
Construction Technologies
Construction Technologies (E . E . )
Control and Automation Technologies
Control and Automation Technologies (E . E . )
Machining
Accountancy and Taxing Applications
Accountancy and Taxing Applications (E . E . )
Marketing
Drilling Technologies
Drilling Technologies (E . E . )
* E . E . = Evening Education

Quotas
120
120
80
40
40
40
40
80
40
40
100
100
80
60
60
60
40
40
40
40
40
40
80
40

Table 1 : Kaman MYO, Programmes and the Quotas for 2010 (Source: www://osym . gov . tr).

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According to the foregoing data, the total quota is 1460 and in the event that all the quotas are full, the total
number of the students will be nearly 2200 and will be almost 3000 a year later.

Literature Review
A group of people who come together to achieve a goal forms the organizations. To achieve the goals of
the organizations is possible through the management activities. It goes without saying that the goals can be
effectively and productively achieved thanks to the existence of the human factor. Akyol (2008)
The success of the human resources applications carried out in education organizations directly affect
the quality, motivation and performance of the instructors which, as a result, plays an important role in forming
the future of the society by directly affecting the quality of the individuals educated. Akyol (2008)
The human resources management, in the broadest term, can be defined as a strategic and consistent
approach which is developed in order to manage the most important entity of an organization; that is the
employees. The human resources management is the management of all activities made in order to acquire and
improve a high work force in and organization , ensure and keep their motivation high. Barutçugil (2004)
The instructors at school are in a dynamic structure which is continuously changing, innovating, and
improving. In the difficult process of becoming an information society, the movements towards a change based
on information has also changed the expectations of people from the education. Today, the traditional way of
education is insufficient; planning radical changes and transformations in the education policies, their goals, and
the structure and functions of the education organizations and in education programmes have become an urgent
need. Arslan and Eraslan (2003).
The function of the education system and realization of its functions as required are only possible with
an organization with a sufficient capacity and quality. Balcı (2000)
Within the scope of the human resources management practices to be carried out in the education
institutions, it should be first determined what kind of people and skills are required for the management of the
school in an effective way and the conduct of the training activities in a successful way. The required human
resources should be selected carefully and an effective method should be developed in order to carry out this
selection successfully. The activities made at school should be analyzed, the content of the work, the requirement
for them, and the order of operations should be determined in this respect. New systems should be created in
order to increase the performance of the personnel at school. The professional future of personnel should be
planned, thus their professional improvement should be guided. The human resources that the school has in it
should be used in the fields where they can be successful, that is the right person should do the right thing. The
personnel should continuously be ensured to improve themselves. In order for the human resources at school to
be managed in an effective way, the school should keep in touch with other schools and institutions and should
have information about their human resources as well. New plans should be made to meet the need for a human
force for the future and the personnel should be educated for the needs of the future. Erdoğan (2003)
The most important factor which leads the way to the success or failure of an education organization is
the determination of the instructors in the right way, in the right time and with the right qualifications and the
selection of the most useful one among them is individually an important issue. Therefore, finding, selecting and
placing an employee which is a dynamic function of the human resources management, which is the employment
is the hardest but the most important function of the process. Akyol (2008)
The most general and suitable definition for career can be summarized as this: “Career is the experience
gained and the series of attitudes and behaviours about activities that a person has acquired all his life”. The
process to achieve the goals that individuals determined in their professional or social lives and the experiences
which came along through the programmes they applied in this process play an important role in terms of
structuring their career. insankaynaklari.com (2008).
Career development is the collection of all activities that individuals made in order to achieve their
career goals all their lives. Barutçugil (2004)
According to the results of a survey conducted among the instructors, although their needs and expectations
are different, these common facts play an important role in motivation and job satisfaction. Ataklı (1996):
• A good and fair salary
• Good working conditions
• Promotion and improvement opportunities
• Appraisal of success
• A job guarantee
• Understanding of personal problems
• The nature of the job as interesting and meaningful.

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The key factor in increasing the motivation of the instructors is to ensure the efficacy at school . The school
management should lead the instructors to cooperation and to increase the quality of the education in their
departments or classes. Increasing the responsibility for education, decreasing the loss of a job, developing
objective standards and encouraging groups are the main fields that are required for being successful. The
school managements should create a bond between the super-units and subunits. Emmanuel (1998)

Method (The Structure of the Model Created)
Within the scope of the human resource practices to be realized in the education organizations , it has
been determined what kind of people and skills are required in order to manage the Kaman MYO effectively and
carry out the educational activities successfully and thus the main philosophy has been formed .
In order to apply for the exam of being an instructor at a Vocational School of Higher Education in
Turkey , one needs to finish a four year degree with a higher score than the average , and to work in his/her
field for at least two years or to do a master’s degree programme . In addition, one is required to receive a score
over a minimum passing score from the Academic Personnel Master’s Degree Exam (ALES) and to have a good
score from the foreign language exams that are conducted with a centralized system. The candidates who can
meet these requirements are subject to a written exam and an interview and then they are employed.
In the process of recruitment , in addition to the requirements for being an instructor , the applicants
are told that the ones who want to make an academic career will be preferred , and therefore they will have an
off day in a week for their master’s degree or doctorate’s degree studies , and the ones who finish their
doctorate’s studies will be an instructor (as an associate professor) as practicable as possible . As we believe
that “Only going into the classroom and teaching” is obsolete even at secondary education institutions , we have
paid attention to choose the applicants who may do academic studies , participate in social and group activities ,
and hence contribute to the vision of Kaman MYO .
In order to realize our aims , it is considered that the most productive choice is to combine the efforts of
an experienced academic staff that is devoted to the vision of Kaman MYO and has high academic values with
the efforts of a junior personnel who can achieve high motivation by learning the goals and thus a good training
structure has been planned for the junior staff .
Since Kaman MYO is affiliated to a state university, the charges are determined as per the public
regulations. As good working conditions are required for the highly qualified staff employment, lodgings are
provided for most of our academicians who come out of Kaman. New office furniture, telephone and internet
connections have been supplied in their offices which are clean and spacious; ring services are also available
from the lodgings to the school buildings.
The rewarding of the instructors has been paid great attention. All the instructors have been guaranteed
that they will have their jobs on condition that they improve themselves scientifically and educationally. All
kinds of academic studies either in Turkey or abroad have been supported. The new instructors have also been
informed that their personal problems will be tried to be overcome in Kaman MYO where we try to build close
relationships.
We state that the relationship between the academicians and the students is warm but the factor of
respect shall never be forgotten. We also state that Kaman MYO is a model school and is known as mentioned
in its vision and will have a wide range of products which is highly prestigious and preferred. Therefore, the
instructors who will contribute to the provision of these products will be also highly respected and demanded.
This great target has been internalized by all staff, and the academic and administrative studies have continued
and will continue until late at nights.
New systems have been created for increasing the performances of the young instructors, their
professional futures have been planned, and therefore their improvements in their careers have been guided. The
current human resources have been led to activities where they can use and improve their skills. The personnel
should always be ensured that they will improve themselves. Other institutions and schools have been contacted
and cooperative opportunities have been created. Plans for meeting the human force which is required for the
future have been made; the academic staff has been trained for the future needs.
The ages, and the rates of finishing master’s and doctorate’s degree of the academic staff of Kaman
MYO are listed in Table 2.

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�2nd International Symposium on Sustainable Development, June 8-9, 2010 Sarajevo

No

Age

MS

PhD

52

OK

OK

55

OK

OK

35

OK

44

OK

OK

42

OK

OK

34

OK

OK

27

OK

28

OK

28

OK

27

OK

29

OK

26

OK

25

OK

30

OK

26

OK

33

OK

29

OK

29

OK

OK

28

OK

OK

27

OK

OK

29

OK

OK

28

OK

31

OK

29

OK

30

OK

33

OK

28

OK

33

OK

32

OK

30

OK

26

OK

26

OK

29

OK

33

OK

27

OK

32

OK

27

OK

28

OK

33

OK

40

Ali Đhsan KARAYĐĞĐT
Orhan ÇAKIRER
Engin YÜKSEL
Ebubekir KILIÇ
Latif Onur UĞUR
Zeynel BAŞIBÜYÜK
Ahmet AKSOY
Kadir Can BURÇAK
Muhammed ARSLAN
Mustafa KILIÇ
Mehmet Fatih TEFEK
Serdar KORKMAZ
Đlknur BEKEM
Emel Elif YARDIMCI
Yasemin TAŞKIRDI
Sertan AYTAÇ
Göktürk ÖZTÜRK
Gökhan ÖZEN
Serap YALÇIN
Berna ÖZALP ÖZEN
Ediz Sadık KANBĐR
Engin EKDUR
Faih BALLI
Yasin KAPLAN
Emre ĐNCE
Gökhan HEPYÖRÜK
Şekip Esat HAYBER
Barış Gürcan HAKANOĞLU
Bekir YURDUGÜZEL
Serkan KESER
Mücella ÖZBAY
Ali ÖZTÜRK
Gökhan EKĐNCĐOĞLU
Halil Özcan ÖZDEMĐR
Fatih KESKĐNKILIÇ
Başar ALTUNTAŞ
Mustafa Halid KARAARSLAN
Öznur ŞAKLAK
Ramazan LEYLEK
Murat AVAN

39

OK

41

Mehmet GÜCÜYETMEZ

30

OK

42

Nilüfer KAMAN
M . Akif ALKAYA
Ceyda ZERENAY
Yasin YAYLAR
Suzan ERTÜRK
Filiz GEMĐCĐ
Tuğba AKKOYUN
Total
Average

28

OK

44

OK

24

OK

27

OK

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39

43
44
45
46
47
48

Name , Family Name

31

OK

28

OK

28

OK

OK

OK

OK

OK

OK

1497
31 . 2

Table 2 : Education and Ages of the academic staff of Kaman MYO.
According to these data, there aren’t any academicians who did not do their master’s degree and the
average age level (together with the instructors) is 31.2 .
Creation of a free working environment where all kinds of positive efforts put forth by the young instructors
in the educational, cultural, arts, sport and social fields, is supported .

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Applications and Acquisitions
Some of the applications made in approach origin in the method section and some of the improvements
and acquisitions have been listed as follows:
Kaman MYO Total Quality Management System
Kaman MYO has created and certified a Total Quality Management System since 2007. The system
which is dynamically improving with the detection and development efforts made every year and which is
applied in a wide range of fields can be found in many different departments of a great number of universities in
Turkey. The vision of Kaman MYO is: “To be a vocational school of higher education which is a leader in
Turkey and which is known all over the world”. Its mission is: “To train the technicians whose level of
knowledge and skills is very high, who received a modern education, who are required in both public and private
sector in the market needs, and who can fulfil their duties at a demanded quality level and on time”. See (Fig. 1)

Figure 1: Certificates of Total Quality Management System.
Publications: Handouts, posters, articles, reports, books
Over the last 3 years, the academicians at Kaman MYO have had 7 papers that are browsed at Science
Situation Index, 9 international congress notices, 36 articles in national scientific journals with referees, 8
national congress poster notices, 4 national sector reports and three scientific course books published. The
instructors of Kaman MYO Construction Programme have prepared a book which has been published by Turkish
Technicians Association and which is based on “The Profession of Civil Technician and Training of Civil
Technicians” as a precedent in Turkey; and they have been given a letter of appreciation for their efforts by the
Turkish Technicians Association .
Courses, seminars, conferences, and interviews outside Kaman MYO
The academicians at Kaman MYO have given training courses through conferences and seminars at the
Ankara, Kayseri and Eskisehir branches of Chamber of Civil Engineers, at the central office of Turkish
Contractors Association (TMB), at the central office of Turkish Employers’ Association of Construction
Industries (INTES). They have also held conferences at Cicekdagi MYO University of Ahi Evran and at
Ministry of Justice Kaman Prison.
Courses, seminars, conferences, and interviews at Kaman MYO

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In addition to the training courses in the fields of AutoCad , Net Cad , DGS Mathematics , Total
Quality Management , MS Project and planning , a great number of seminars , conferences , and interviews
were organized with the participation of experts in the sectors , public servants and the managers of professional
unions . Among these comes the Branch Manger of Building Control by Ministry of Housing, Chairman of
Ankara Project Management Association, Chairman of Union of Building Control Institutions, and the members
of the Executive Committee of Turkish Technicians Association.
Theatre Club, Novel Club
In addition to the stage plays that the Theatre and Novel Clubs at Kaman MYO have in Kaman, they
have successfully performed 4 different plays and poem concerts with 6 different themes and contents at
Performance Hall of University of Ahi Evran, Mucur MYO, Cicekdagi MYO, Kirsehir Governorship, Kirsehir
Prison and Kaman Prison many times. See (Fig. 2)

Figure 2: Views from different performances, news from newspapers atc.
Performances of Turkish Folk Music Club, Rock Music Club, Folk Dances Club
After the regular concerts the Turkish Folk Music and Rock Music groups which were established in
2009 give in Kaman, they were asked to take the stage in the Spring Festivals and Graduation Ceremonies of
University of Ahi Evran and they also passed the elections for the Rock Music Festival to be held among all
universities in Turkey and a competition organized by a national music television. The Folk Dances Club
performed waltz as well as the Azerbaijani and Caucasian dances.
Celebration programmes of the national days
With the performances of the art groups at Kaman MYO have so far presented and will present Kaman
MYO at the celebration programmes for the national days/festivals (such as 18th March , 19th May , 23rd April
29th October) prepared within the body of Kaman MYO and University of Ahi Evran , Kaman Governorship ,
Kirsehir Governorship and Kirsehir Municipality .
Scientific, cultural and social expeditions abroad
Academicians and students at Kaman MYO have participated to represent Turkey and the University of
Ahi Evran in academic, cultural and social activities held in Italy, Poland, Slovakia, Austria, Belgium and
Netherlands for the last two years and have contributed to the cultural integration activities. Furthermore, the

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academicians of Kaman MYO made speeches about disarmament, decreasing the drug abuse, and nuclear wastes
at Headquarters of the European Union.
Technical Excursions in Turkey
A great number of vocational and technical excursions have been organized to a lot of production
plants, industrial plants, administrative centres, application units and fairs at local and international level so that
the students have been given the opportunity to learn more about the fields they are studying and the production
stages and to meet the experts in the professional sector practices. Among the places which have been visited so
far comes Hirfanlı Hydroelectricity Plant , Kaman-Ankara Divided Highway Constructions, Karabuk Iron and
Steel Plant , Kayseri Organized Industrial Site, Tuyap Istanbul Electronics Fair, Ankara Construction Fair, Izmir
Natural Stones Fair, Production Plants of Milk, Meat and Oil, Cement factories, Ready Mixed Concrete Plants,
and numerous construction sites. See (Fig. 3)

Figure 3: Technical Excursions.
Social and cultural excursions
Excursions based on architectural history of Safranbolu Historical Houses, on culture of Nevsehir
Hacibektas Veli Mausoleum and on history of Kaman Archaeological Museum; trips to near touristy and natural
places have been held on holidays.
MYO and MYO Student congresses/ symposiums
For the first time in Turkey, two Kaman MYO students presented their notices which they had
previously prepared with their instructors in front of more than 600 scientists at an international symposium, and
this was on the news both on television and in the newspapers and Mayor of Kaman and the District Governor of
Kaman received the notices of appraisal from Kirsehir Governor and District Governor of Izmir Seferihisar
where one of the students reside. These two students at Kaman MYO also made presentations at the MYO
congress which was held in Inegol, Bursa in 2009. Ten of the abstract presentations which they had prepared
with their instructors were accepted for the National MYO Students Congress to be held in Duzce in November,
2010 which is the first of these congresses. The contributions of the instructors who take part in the organization
of these congresses and symposiums as well as the science councils and who spend their time with their students
so that they can improve themselves and produce on scientific and social fields at an international level are of
great importance .
Laboratories
The laboratories which are required by the departments giving technical training and they are still being
improved. Among these comes the Microbiological Food, Chemical Food, Natural Structural Stones,
Construction Material, Concrete, Electronics and Correspondence, Printed Circuit, PLC, Electronic laboratories.
See (Fig. 4)

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Figure 4: Different laboratories.
School buildings, Structure of Cultural Centre, new TOKĐ building
Kaman MYO, which continued its educational activities in an old building which used to be a pensioner
house until two years ago, in line with its growth in the number of departments, instructors and students, had a
new school building of 12 classrooms in February, 2008 and had a bigger building of 24 classrooms (TOKI
Building) in April, 2010 as a result of giving the first building back to the Ministry of National Education and
still continues its activities in these buildings . In addition to these buildings, thanks to the protocol signed, the
operation rights of the Kaman Culture Centre were transferred to Kaman MYO. See (Fig. 5)

Figure 5: School buildings.
News in the newspapers (at local and national levels)
The local press makes significant contributions to Kaman MYO and makes news on the local, national
and international success, social, cultural, artistic and sports activities of Kaman MYO. This news takes part in
the press of Kirsehir and national press.
Columns at local newspapers
The academicians at Kaman MYO continue their activities in order to open up the horizon of people in
Kaman and Kirsehir, widen their scope of perspective and increase their intellectual level. Various academicians
write literature critics on the column titled “Why should we read, and what should we read?” by taking turns and
try to increase the level of reading habits and quality of critics.
E-student newspaper
On the Kaman MYO web site, an e-student newspaper, named “Sesimiz (Our Voice)” which includes
the news and columns of students and the interviews with their instructors is published on a monthly basis.
Academic studies about Kaman
The instructors of Kaman MYO make and publish scientific researches not only in their fields of
expertise but also local elections , production of walnuts and milk , housing constructions of the construction
firms and their marketing activities , relationships between the Kaman public and Kaman MYO . These

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activities receive great attention and support by both Kaman and Kirsehir community, Kirsehir governorship,
Municipality and Chamber of Commerce, and the fields of study can be broadened.
Publication of scientific journal (Ahi Evran University, Journal of Vocational Sciences)
Kaman MYO is trying to complete the works related to the publication of a refereed, periodical, and
scientific journal (Ahi Evran University, Journal of Vocational Sciences) which will be published within the
body of Kaman MYO and it will soon be published . Therefore a dynamic platform which the publications both
from Turkey and from all over the world will be met on can be formed, the developments and researches
particularly from Vocational Technical Training will be contributed.
Competitions for Poems and Stories, books issued
Competitions for writing and reading poems and writing stories are organized open to the participation
of all students of Kaman MYO, the creators of the works which are placed are awarded with a ceremony and
their works are issued in books. Therefore, we try to improve the cultural and artistic capabilities of our students
and try to eternalize their works by publishing them.
Sports activities
In addition to the football, basketball and volleyball matches organized within the body of Kaman MYO
every year, the students take part in the championships held among the faculties, Vocational Schools of Higher
Education and Vocational Schools within the body of University of Ahi Evran.
Protocols signed with the industry
The protocols signed with the industrial firms are for the aim of contributing to the internship and employment
of students. All of the students of department of drilling works are ready even while they are studying; and the
training places of the students of the departments of construction, food and computing programmes and
employment of some of them will be realized based on the agreements made. We still continue our efforts to
make new protocols with different industrial firms for the other departments.
Construction of a Hotel and the University Campus; Technical consultancy services and concrete tests
The Construction Programme within the body of Kaman MYO, thanks to both its modern laboratories
and the experience and knowledge of its academicians, gives technical consultancy service in the construction of
a five star thermal hotel whose construction is still being made in Kirsehir as well as conducts and reports a great
number of tests –foremost among these comes the concrete tests- within the scope of the construction of the Ahi
Evran University Campus which is still being carried out in Kirsehir. In addition it gives consultancy in the
planning and control of construction projects.
Establishment of a Graduate Supervision System
Efforts to set up a graduate supervision system within Kaman MYO is still being made with great
speed; a new vice-director is going to be appointed and a new computer software is going to be prepared in this
respect .

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Getting the experts in Kaman and near towns to give lectures
Kaman MYO is also benefitting from the knowledge and experience of the experts in technical and
administrative fields. Professional pragmatics and administrators give lectures for a term in different
programmes. That the students know more about their sectors and the qualities they are required to have is
aimed. This has also become very prestigious among the professionals in Kaman and Kirsehir and the demand
for giving lectures has continuously increasing.
Kaman MYO’s role in the administration of the township of Kaman
The academicians of Kaman MYO take part in various councils in the administration of Kaman. In
addition to their roles in education, they continue to give support to the local administrations. Some of these
councils are Human Rights Council, Public Education Commission, Kaman Strategic Planning Council, etc.
Library
Kaman MYO, which has a big library, is continuously improving this library where both the
academicians and the students can benefit from national and international publications. With an agreement made
with the Kaman Central Library, all the students have become a member of this library and benefit from this
library and its good conditions for their studies and social activities . See (Fig. 6)

Figure 6: Library
Teaching Foreign Languages
The official foreign language which is being taught at state universities in Turkey is the English
language. However, other than English, Kaman MYO is providing German, Russian and Japanese classes and
courses either within the curriculum or additional courses. The students are aimed to be versatile and know the
values of different cultures.
Students’ opinions
As a result of the polls carried out about equipments, education, social facilities, personal development,
etc. , a vast majority of the students expressed that they are satisfied with their school, instructors and the level of
the education they are receiving. The number of students who responded to the question whether they would like
to study at a different Vocational school of higher education does not exceed 5% of the total number of students.
Since the age gap between the instructors and the students is not very big, the students express that they are
happy with their brother-sister relationship they have with their instructors and with their instructors’ efforts.
Supporters
Deputies of province of Kirsehir , Kirsehir Governorship , Kaman Municipality , Kaman District
Governorship, Rectorship of University of Ahi Evran, Ministry of National Education (MEB), local press,
Kaman Chamber of Commerce and benefactors support the development of Kaman MYO and contribute its
becoming well-equipped, construction of new residence halls , improvement of social and cultural facilities with
great satisfaction .

Results and Evaluation
While the quality of the education is being improved through not only the physical development but
also the seminars, conferences and technical excursions organized, and the modern laboratories established, a
great number of scientific articles, notices, scientific reports, original and supplementary course books produced
contributed to the scientific development of the school. Within the scope of the European Union grant projects,
numerous instructors and students participated in eight different countries and projects, and social integration has
been increased thanks to the EU higher education and youth projects. The success gained in the sports activities
within the vocational school of higher education and the university , the soft music , folk music and rock music

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concerts prepared by the student groups , high artistic values at theatre plays , musicals , and poem concerts ,
publication of the works of the students who were placed and got an honourable mention in the story and poem
writing competitions, have all been realized thanks to the young and dynamic academic staff . Kaman MYO,
which is at the top of the list among the 550 vocational schools of higher education in Turkey in a short period of
time, has created a good education model.
In order to achieve its goal “to be a leader in Turkey and widely known in the world”, the combination
of an experienced academic staff that is devoted to the vision of Kaman MYO and has high academic values
with the efforts of a junior personnel whose career goals have been clarified is deemed to be the best choice, in
addition to the training of the junior staff together with the aforementioned acquisitions, we believe that the
choice made and applications conducted are suitable.
All kinds of achievements we made are thanks to the great efforts and devoted approach of the young
academic staff. That this brings positive results to Kaman MYO as a model is well appreciated. Undoubtedly, all
the achievements we have made so far have an important role but they are not sufficient for our goal. The
progress we have made should be improved with the development efforts.
It is expected that the development of this model with a sustainable sense of quality and taking it into
account in the structuring to be carried out by other higher education institutions will have positive contributions
to the global vocational and technical training efforts.
References
Akyol, B. (2008). The Effect Of Human Resources Functıons On Teacher Performance In Schools, Ms Thesis,
Insitude of Social Sciences, Đstanbul, Yeditepe University, (in Turkish)
Barutçugil, Đ. (2004). Strategic Human Resource Management, Kariyer Pulishing, Đstanbul, p.32.
Arslan, M., Eraslan, L. (2003). New Education Paradigm and Necessity of the Conversion in Turkish Education
System, The National Education Magazine, http://yayim.meb.gov.tr/dergiler/160/arslan-eraslan.htm, 160.
Balcı, A. (2000). Organization and Management of Turkish Education System in New Millenium, Journal of
Educational Management in Theory and Practice, Ankara, 24(6), p.495-508.
Erdoğan, Đ. (2003). School Management and Instructional Leadership, 4. Edition, Sistem, Đstanbul, p. 114-115.
Individuals and Career Management Concept, (2008). www.insankaynaklari.com
Ataklı, A. N. (1996). Importance of Personel Qualities and Motivations to work in Lecturers, Contemporary
Education Review, 221, s. 23-26.
Emmanuel N.C. (1998). How Community College Administrators Can Improve Teaching Effectiveness,
Community College Journal of Research and Practice, 22, s.1-6.

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�</text>
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                <text>An Education Structuring Model in Vocational and Technical Education  with The Orijins of Young Lecturers: Kaman Vocational School</text>
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Uğur, Latif Onur</text>
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                <text>In this study, it is discussed that a breakthrough approach to education with young  faculty members in vocational technical education. Kaman Vocational School which was  connected another university before, connected Kırsehir Ahi Evran University with a decision  received in its foundation date in 2006. Continuing its tarining activities given 160 students three  departments, two permanent faculty members and the others coming from nearby provinces;  Kaman Vocational School has gained acceleration with a department at four years, students over  1700 and 48 faculty members and the radical decisions given by university management. Not  only physical growth but while improving the quality of education with seminars, conferences,  technical tours, modern laboratories, important scientific progress achieved by numerous  scientific articles and reports, papers, original and alternative textbooks. Under European Union  (EU) projects, it was participated with lots of faculty members and students to eight different  countries and projects; the social integration increased with the EU higher education and youth  arrangements. Successes in sporting activities held within the Vocational School and the  university; higher artistic levels in light music, folk music, rock music concerts, theatres, musical  and poetry nights prepared by student groups; publishing students’ works that take a degree and  honorable mention in story and poetry writing competitions are all provided with the  contributions of young and dynamic academic staff. Among over 550 Vocational School in  Turkey, Kaman Vocational School that catches on the tops in a short time, constituted a sample  training model. In the study, in this model and the role of young teaching staff and the added  value they provide within this model is discussed with the help of related data.</text>
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