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                    <text>Journal of Economic
and Social Studies
Volume 67

Number 2

2016
Fall 2018

Refereed Articles
55

Role ofofthe
State in
Financial
Sector Development
and
Growth: Stocks
Performance
Moving
Average
Investment
Timing Strategy
inAchieving
UK Stock Pro-Poor
Market: Individual
versusEvidence
Portfoliosfrom Bosnia and Herzegovina
Muhammad
Ishfaq Ahmad,
Wang Ghohui,
Mudassar
Amra Babajić
and Meldina
Kokorović
Jukan Hasan, Anika Sattar, Muneeb Ahmad and Ramiz Ur
Rehman
27
Trade liberalization and product structure: The case of Western Balkans
22 LabourEdward
MarketMolendowski
Transition Differences
Natives and Immigrants in EU Economies
and Łukaszbetween
Klimczak
Valerija Botrić
43
Key Success Factors for Sustainable Strategic Information Systems
56 Internal
Migration
Social Identity
Construction:
Implications for Prejudice and Stigma in Albanian
Planning
andand
Information
Technology
Infrastructure
Post-socialist
Society
Zana Pekmez
Merita H. Meçe

Journal of Economic and Social Studies

JECOSS

Journal of Economic
and Social Studies
JECOSS

57
Trends and Challenges of Female Unemployment in the Republic of Macedonia:
81 Determinants of the Financing Obstacles Faced by SMEs: An Empirical Study of Emerging Economies
A Regional Comparative Study
Mirgul Nizaeva and Ali Coşkun
Remzije Rakipi and Shpressa Syla

109

List of Reviewers for this Issue

Volume 67

Number 22
Number

Number 2
Number

129 List of Reviewers fot this Issue
81
Investigating the Drivers of Choice Behavior in Tourism: Corporate Image, Perceived Risk and Trust
Interactions through Reputation Management
Mesut Bozkurt and Emrah Özkul

Volume 67

100 Bilateral Intra-Industry Trade in Country Characteristics Context: The Case Study of Trade of Bosnia
79 and Herzegovina
Public Procurement
System in Service for Strengthening the Market Economy
with Croatia
in
Bosnia
and
Herzegovina
Snježana Brkić
Merim Kasumović, Sanela Meholjić-Kalajdžić and Harun Meholjić

Fall 2016
2018

Print ISSN: 1986 - 8499
Online ISSN: 1986 - 8502

Fall 2016
Fall
2018

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                    <text>�FORENSIC

GENETICS
Theory and Application

�FORENSIC GENETICS
THEORY AND APPLICATION

Damir Marjanović, Ph.D.
Dragan Primorac, M. D. , Ph.D.
Serkan Doğan, Ph.D.
Publisher:
International Burch University, Sarajevo, Bosnia and Herzegovina
Printed by:
Sabah Print d.o.o.
DTP-Design:
Veysel Cebe
Circulation:
500 copies
Place of publication:
Sarajevo, Bosnia and Herzegovina
Copyright:
International Burch University, 2018
______________________________________________________________________
CIP - Katalogizacija u publikaciji
Nacionalna i univerzitetska biblioteka
Bosne i Hercegovine, Sarajevo
577.21:340.64
MARJANOVIĆ, Damir
Forensic genetics : theory and application / Damir Marjanović, Dragan Primorac, Serkan
Doğan. - Sarajevo : International Burch University, 2018. - 289 str. : ilustr. ; 28 cm
Bibliografija uz tekst. - Registar.
ISBN 978-9958-834-63-9
1. Primorac, Dragan 2. Doğan, Serkan
COBISS.BH-ID 26376198

�FORENSIC

GENETICS
Theory and Application
Damir Marjanović, Ph.D.
Professor of Forensic Genetics and Molecular Anthropology, Department of Genetics and
Bioengineering, International Burch University, Sarajevo, Bosnia and Herzegovina
Scientific Associate, Institute for Anthropological Research, University of Zagreb, Zagreb,
Croatia

Dragan Primorac, M.D., Ph.D.
President of the Board of Trustees, St. Catherine Specialty Hospital, Zagreb and Zabok, Croatia
Adjunct Professor of Forensic Science, Eberly College of Science, The Pennsylvania State
University, University Park, PA, USA
Adjunct Professor of Forensic Science, The Henry C. Lee College of Criminal Justice and
Forensic Sciences, University of New Haven, West Haven, CT, USA
Professor of Pediatric Medicine, Medical School, University of Split, Split, Croatia
Professor of Pediatric Medicine, Medical School, University of Osijek, Osijek, Croatia
Professor, Medical School, University of Rijeka, Rijeka, Croatia

Serkan Doğan, Ph.D.
Assistant Professor of Population Genetics and Forensic Genetics, Department of Genetics and
Bioengineering, International Burch University, Sarajevo, Bosnia and Herzegovina

�Contributors
Adna Ašić
Department of Genetics and Bioengineering
Faculty of Engineering and Natural Sciences
International Burch University
Sarajevo, Bosnia and Herzegovina

Monia Avdić
Department of Genetics and Bioengineering
Faculty of Engineering and Natural Sciences
International Burch University
Sarajevo, Bosnia and Herzegovina

Serkan Doğan
Department of Genetics and Bioengineering
Faculty of Engineering and Natural Sciences
International Burch University
Sarajevo, Bosnia and Herzegovina

Mirela Džehverović
Institute for Genetic Engineering and
Biotechnology
University of Sarajevo

Mirsada Hukić
Larisa Bešić
Department of Genetics and Bioengineering
Faculty of Engineering and Natural Sciences
International Burch University
Sarajevo, Bosnia and Herzegovina

Lada Lukić Bilela
Department of Biology
Faculty of Natural Sciences and Mathematics
University of Sarajevo
Sarajevo, Bosnia and Herzegovina
BIOSPEL – Biospeleological Society of Bosnia
and Herzegovina
Sarajevo, Bosnia and Herzegovina
ADIPA – Society for Research and Conservation
of Croatian Natural Diversity
Zagreb, Croatia

Elma Ferić Bojić
Department of Genetics and Bioengineering
Faculty of Engineering and Natural Sciences
International Burch University
Sarajevo, Bosnia and Herzegovina

Institute for Biomedical Diagnostics and
Research NALAZ
Sarajevo, Bosnia and Herzegovina
Academy of Sciences and Arts of Bosnia and
Herzegovina
Sarajevo, Bosnia and Herzegovina

Damir Marjanović
Department of Genetics and Bioengineering
Faculty of Engineering and Natural Sciences
International Burch University
Sarajevo, Bosnia and Herzegovina
Institute for Anthropological Research
University of Zagreb
Zagreb, Croatia

Enisa Omanović Mikličanin
Faculty of Agriculture and Food Science
University of Sarajevo
Sarajevo, Bosnia and Herzegovina

Imer Muhović
Ascidea Genomics &amp; Bioinformatics CRO
Barcelona, Spain

Jasmina Čakar
Institute for Genetic Engineering and
Biotechnology
University of Sarajevo
Sarajevo, Bosnia and Herzegovina

Lejla Kovačević Mulahasanović
Center for Genomics and Transcriptomics,
CeGaT, GmbH
Tübingen, Germany

�Table of Contents
and Authors
Dragan Primorac
St. Catherine Specialty Hospital
Zagreb and Zabok, Croatia
Eberly College of Science
The Pennsylvania State University
University Park, PA, USA
The Henry C. Lee College of Criminal Justice
and Forensic Sciences
University of New Haven
West Haven, CT, USA
Medical School
University of Split
Split, Croatia
Medical School
University of Osijek
Osijek, Croatia
Medical School
University of Rijeka
Rijeka, Croatia

Edited by:
Damir Marjanović, Ph.D., Dragan Primorac, M. D.,
Ph.D., Serkan Doğan, Ph.D.
1.

Introductory concepts and facts
Damir Marjanović, Dragan Primorac, Serkan
Doğan

2.

Historical Development of Forensic Genetics
Damir Marjanović, Dragan Primorac, Serkan
Doğan

3.

The variability of DNA and molecular markers in
forensic genetics
Serkan Doğan, Adna Ašić, Dragan Primorac,
Damir Marjanović

4.

Basic models and phases of the process of DNA
analysis
Damir Marjanović, Jasmina Čakar, Lejla Smajlović
Skenderagić, Larisa Bešić, Serkan Doğan, Dragan
Primorac

5.

Application of lineage markers and the X
chromosome analyses in forensic genetics
Adna Ašić, Larisa Bešić, Lejla Kovačević
Mulahasanović, Elma Ferić Bojić, Serkan Doğan,
Dragan Primorac

6.

Technological development trends in forensic
genetics
Lejla Smajlović Skenderagić, Damir Marjanović,
Serkan Doğan, Imer Muhović, Larisa Bešić, Adna
Ašić

7.

Basic biostatistical rules in forensic genetics
Serkan Doğan, Imer Muhović, Adna Ašić, Larisa
Bešić, Dragan Primorac, Damir Marjanović

8.

DNA database and missing persons identification
Damir Marjanović, Imer Muhović, Monia Avdić,
Serkan Doğan, Lejla Smajlović Skenderagić

9.

Forensic DNA analysis of plant and animal
biological traces
Larisa Bešić, Imer Muhović, Adna Ašić, Jasmina
Čakar, Lada Lukić Bilela, Mirela Džehverović,
Monia Avdić

Lejla Smajlović Skenderagić
Department of Genetics and Bioengineering
Faculty of Engineering and Natural Sciences
International Burch University
Sarajevo, Bosnia and Herzegovina

10. Food Forensics
Enisa Omanović Mikličanin
11. Microbiomes as tools in human identification
Monia Avdić, Lejla Smajlović Skenderagić,
Mirsada Hukić
12. Supplement: Procedures for the collection and
labeling of biological traces meant for DNA
analysis
Imer Muhović, Adna Ašić, Larisa Bešić, Mirela
Džehverović, Damir Marjanović

�Contents
Chapter 1
INTRODUCTORY CONCEPTS AND FACTS ........................................................... 15
1.

2.

Fundamentals of forensic science ............................................................................................................ 19
1.1. What is forensic science? .................................................................................................................... 19
1.2. Fields of forensic science .................................................................................................................. 19
1.3. Classical criminalistics methods for the identification of human individuals
and the individualization of human traces ......................................................................................... 25
1.3.1. Identification and individualization via phenotypic marker analysis ...................................... 27
1.3.2. Identification and individualization using fingerprints ............................................................ 29
1.3.3. Identification using dental records .......................................................................................... 30
1.3.4. Identification and individualization using skeletal remains .................................................... 34
Basic models of molecular genetics ......................................................................................................... 38
2.1. The cell ............................................................................................................................................. 38
2.2. Chromosomes ................................................................................................................................... 39
2.3. Deoxyribonucleic acid (DNA) .......................................................................................................... 42

Chapter 2
HISTORICAL DEVELOPMENT OF FORENSIC GENETICS ................... 47
Chapter 3
VARIABILITY OF DNA AND MOLECULAR MARKERS IN
FORENSIC GENETICS .............................................................................................................. 57
3.1. Variable Number Tandem Repeats – VNTR molecular markers .............................................................. 60
3.1.1. RFLP analysis of minisatellite markers .......................................................................................... 61
3.2. STR – Short Tandem Repeat molecular markers ..................................................................................... 62
3.2.1. The structure and nomenclature of STR markers ........................................................................... 63
3.2.2. Standard systems of STR loci ......................................................................................................... 64
3.2.2.1 CODIS (COmbined DNA Indexing System) loci ................................................. 64
3.2.2.2. INTERPOL Standard Set of Loci (ISSOL) ........................................................... 65
3.2.2.3. European Standard Set of Loci (ESS) ................................................................... 65
3.3. Alu repeats .............................................................................................................................................. 66
3.4. SNP – Single Nucleotide Polymorphism .................................................................................................. 66
3.5. mtDNA hypervariable regions ................................................................................................................ 70

Chapter 4
BASIC MODELS AND PHASES OF THE PROCESS OF DNA
ANALYSIS ............................................................................................................................................. 75
4.1. Biological traces ........................................................................................................................................ 78

�4.2. Collection and storage of samples .............................................................................................................. 79
4.3. Identification of type of biological trace .................................................................................................... 81
4.3.1. Testing and identification of bodily fluids ....................................................................................... 82
4.3.1.1. Testing and identification of blood traces .............................................................................. 82
4.3.1.2. Testing and identification of sperm traces ............................................................................ 85
4.3.1.3. Testing and identification of saliva traces .............................................................................. 87
4.3.1.4. Testing and identification of urine traces ............................................................................... 88
4.3.2. Testing and identification of hair traces .......................................................................................... 88
4.4. DNA isolation methods ............................................................................................................................. 91
4.4.1. DNA isolation using organic solvents ............................................................................................. 92
4.4.2. DNA isolation using “Chelex® 100” method ................................................................................. 93
4.4.3. DNA isolation using Qiagen method .............................................................................................. 94
4.4.4. Promega DNA IQ™ System ........................................................................................................... 95
4.4.5. Prepfiler™ Forensic DNA Extraction Kit ....................................................................................... 96
4.4.6. Invitrogen ChargeSwitch® System ................................................................................................ 96
4.4.7. Other DNA isolation methods ........................................................................................................ 97
4.5. DNA quantification methods ..................................................................................................................... 98
4.5.1. Determination of DNA quantity in a sample using spectrophotometry ........................................... 98
4.5.2. Determination of concentration, yield and purity of DNA sample using the agarose gel
electrophoresis method ................................................................................................................... 99
4.5.3. Hybridization (slot blot) method ................................................................................................... 100
4.5.3.1. QuantiBlot® Human DNA Quantitation Kit ....................................................................... 100
4.5.3.2. AluQuant™ Human DNA Quantitation System .................................................................. 101
4.5.4. qRT-PCR quantification ................................................................................................................ 102
4.5.4.1. Quantifiler® Human DNA Quantification Kit .................................................................... 102
4.5.4.2. Quantifiler® Duo DNA Quantification Kit .......................................................................... 103
4.5.4.2. Quantifiler™ HP DNA Quantification Kit ........................................................................... 104
4.5.4.3. Quantifiler® Trio DNA Quantification Kit ......................................................................... 104
4.5.4.4. Plexor® HY System ............................................................................................................ 105
4.5.4.5. Investigator® Quantiplex Kit .............................................................................................. 106
4.6. Polymerase Chain Reaction ..................................................................................................................... 107
4.6.1. Basic premises of the PCR and its biochemistry ........................................................................... 107
4.6.2. Optimization of basic parameters in application of PCR in forensic genetics ............................. 109
4.7. Detection of PCR results .......................................................................................................................... 110
4.7.1. Detection of allelic variants on STR loci ....................................................................................... 110
4.7.1.1. DNA profile ......................................................................................................................... 112
4.7.2. DNA sequencing ........................................................................................................................... 115
4.8. Application of autosomal multiplex PCR-based systems in forensic genetics ....................................... 116
4.8.1. Early PCR-based systems ............................................................................................................. 117
4.8.1.1. AmpliFLP® D1S80 PCR Amplification Kit ........................................................................ 117
4.8.1.2. AmpliType® PM+DQA1 PCR Amplification and Typing Kit ............................................ 117

�Contents
4.8.2. Commercial autosomal multiplex PCR-based STR systems ....................................................... 118
4.8.2.1. PowerPlex® 16 System ...................................................................................................... 120
4.8.2.2. PowerPlex® 21 System ....................................................................................................... 121
4.8.2.3. AmpFlSTR® Identifiler® PCR Amplification Kit .............................................................. 122
4.8.2.4. Investigator IDplex Kit ....................................................................................................... 123
4.8.2.5 PowerPlex® ESX and ESI System ...................................................................................... 124
4.8.2.6 AmpFlSTR® NGM and NGM Select PCR Amplification kits ............................................ 126
4.8.2.7. GlobalFiler® PCR Amplification Kit .................................................................................. 126
4.8.2.8. Investigator ESSplex Kit ..................................................................................................... 128
4.8.3. MiniSTR Systems ......................................................................................................................... 128
4.8.4. Direct Amplification STR Systems ............................................................................................... 131
4.9. Most frequent challenges in forensic DNA analysis ................................................................................. 134
4.9.1. Low copy number DNA – LCN DNA analysis ............................................................................ 134
4.9.2 Mixed sample analysis ................................................................................................................... 137
4.9.3 Degraded DNA .............................................................................................................................. 138
4.9.4. Microvariants ............................................................................................................................... 138
4.9.5. Mutations ...................................................................................................................................... 139
4.9.7. Challenges in the interpretation of results in court ....................................................................... 141

Chapter 5
APPLICATION OF LINEAGE MARKERS AND X CHROMOSOME
ANALYSES IN FORENSIC GENETICS ...................................................................... 147
5.1. Y chromosome analysis in forensic genetics .......................................................................................... 150
5.1.1. Cytogenetic structure of the Y chromosome and its genes ........................................................... 151
5.1.2. Application of the Y chromosome in forensic and population genetics ......................................... 152
5.1.2.1. Minimal haplotype .............................................................................................................. 154
5.1.2.2. Commercial Y-STR multiplex kits ..................................................................................... 155
5.2. Mitochondrial DNA analysis ................................................................................................................... 155
5.2.1. Inheritance of mitochondrial DNA ............................................................................................... 157
5.2.2. The importance of mtDNA analysis in court medicine ................................................................ 158
5.2.3. Heteroplasmy ............................................................................................................................... 159
5.2.4 Mitochondrial DNA Haplogroups ................................................................................................. 160
5.2.5 Mitochondrial SNPs ...................................................................................................................... 160
5.3. Characteristics of the X-chromosome and its application in forensic genetics ...................................... 161
5.3.1. Main characteristics of the X chromosome ................................................................................... 161
5.3.2. Cytogenetic comparison of human X and Y gonosomes ............................................................... 161
5.3.3. Genes on the X chromosome and the molecular and genetic determination of sexes ................... 163
5.3.4. X-linked STR (Short Tandem Repeat) Markers ............................................................................ 165
5.3.5. Applications of X-STR Markers in Forensic DNA Analysis ........................................................ 166
5.3.6. Possibilities of the application of X-STR markers in cases of kinship testing ............................... 167
5.3.6.1. Paternity testing on human skeletal remains and their postmortem identification ...................... 167
5.3.6.2. Paternity testing in cases where potential fathers are closely related ......................................... 167

�5.3.6.3. Paternity testing in cases where the potential father is not available .................................... 167
5.3.6.4. Paternity testing in cases of rape and incest ......................................................................... 168
5.3.6.5. Maternity testing ................................................................................................................. 168

Chapter 6
DEVELOPMENT TRENDS IN FORENSIC GENETICS
TECHNOLOGY .............................................................................................................................. 171
6.1. Automation of DNA extraction ............................................................................................................... 174
6.1.1. Promega Maxwell®16 System ..................................................................................................... 174
6.1.2. Maxwell® Forensic Sample Concentrator (FSC) Instrument....................................................... 175
6.1.3. Qiagen EZ1 Advanced Instrument ................................................................................................ 175
6.1.4. Qiagen QIAsymphony® SPInstrument ........................................................................................ 175
6.1.5 Qiagen STAR Q SP Instrument ...................................................................................................... 176
6.1.6. AutoMateExpressTMForensic DNA Extraction System .............................................................. 176
6.1.7. Freedom EVO® ........................................................................................................................... 176
6.1.8. Biomek® 3000 Laboratory Automation Workstation ................................................................... 177
6.1.9. iPrep™ Purification Instrument .................................................................................................... 177
6.2. Development and trends in PCR technology ............................................................................................ 178
6.2.1. Applied Biosystems PCR machines .............................................................................................. 179
6.2.2. Eppendorf PCR machines ............................................................................................................. 180
6.2.3. Rotor-Gene Q ............................................................................................................................... 180
6.2.4. Other PCR machines ..................................................................................................................... 181
6.3. Method and technology development in DNA marker analysis in forensic genetics ............................... 181
6.3.1. Fluorescent labeling and detection ................................................................................................ 181
6.3.2. Development of DNA sequencers and genetic analyzers .............................................................. 183
6.3.2.1. Automatic genetic gel analyzers .......................................................................................... 183
6.3.2.1.1. ABI PRISM 373 DNA SEQUENCER ............................................................... 183
6.3.2.1.2. ABI PRISM 377 DNA SEQUENCER ............................................................... 184
6.3.2.2. Automated capillary genetic analyzers ................................................................................ 184
6.3.2.2.1.ABI PRISM 310 GENETIC ANALYZER ......................................................... 184
6.3.2.2.2. Other systems for molecular marker detection ................................................... 185
6.3.2.3. Recent developments in automatic capillary genetic analyzers .......................................... 185
6.3.2.3.1. Spectrum CE System ......................................................................................... 185
6.3.2.3.2. Spectrum Compact CE System .......................................................................... 185
6.4. New technological fields in forensic genetics .......................................................................................... 186
6.4.1. DNA phenotyping ......................................................................................................................... 186
6.4.2. Genetic methods for determining age and type of biological traces .............................................. 189
6.4.3. Application of INDELs in forensic genetics ................................................................................. 190
6.4.4. Next generation sequencing .......................................................................................................... 191
6.4.4.1. Pyrosequencing ................................................................................................................... 196
6.4.5. Third generation sequencing .................................................................................................. 197

�Contents
Chapter 7
BASIC BIOSTATISTICS RULES IN FORENSIC GENETICS .................. 201
7.1. Mendelian inheritance ............................................................................................................................ 204
7.2. Rules in parentage testing ....................................................................................................................... 204
7.3. Hardy-Weinberg equilibrium ................................................................................................................... 204
7.4. Linkage disequilibrium ............................................................................................................................ 205
7.5. Creating a population database ................................................................................................................ 205
7.6. Paternity testing ....................................................................................................................................... 207
7.6.1. Statistical procedures and paternity testing - Paternity Index or Combined Paternity Index ......... 207
7.6.2. Probability of Paternity (W) .......................................................................................................... 209
7.6.3. Random Man Not Excluded (RMNE) .......................................................................................... 210
7.6.4 Motherless paternity testing ........................................................................................................... 211
7.6.5. Maternity testing ........................................................................................................................... 212
7.6.6. Parentage testing as opposed to forensic DNA analysis ................................................................ 214
7.7 Forensic individualization ........................................................................................................................ 214
7.8. Statistical analysis of mixed and low copy number traces ....................................................................... 216
7.8.1 Statistical analysis of mixed traces ................................................................................................ 216
7.8.2. Statistical analysis of low copy number traces ............................................................................. 216
7.9. Statistical testing of biological kinship .................................................................................................... 217
7.10. Identification of mass disaster victims .................................................................................................. 217
7.11. Statistical rules in the analysis of sex-linked markers ............................................................................ 218
7.11.1. Presenting results obtained by the usage of Y-STR systems ........................................................ 218
7.11.2. Presenting results obtained by the usage of X-STR systems .................................................... 218

Chapter 8
DNA DATABASES ......................................................................................................................... 221
8.1. Criteria for Creating Legislation Regarding DNA Databases ................................................................. 224
8.1.1. Criteria for Profile Archiving ....................................................................................................... 224
8.1.1.1. Profiles of Convicted Felons .............................................................................................. 224
8.1.1.2. Profiles of Suspects ............................................................................................................ 224
8.1.1.3. Profiles Obtained from Traces from the Crime Scene ......................................................... 224
8.1.2. Criteria for Removal of Profiles from the Database ..................................................................... 225
8.1.2.1. Profiles of Convicted Felons ............................................................................................... 225
8.1.2.2. Suspects/Arrested Persons .................................................................................................. 225
8.1.3. Sample Storage ............................................................................................................................ 225
8.2. Forensic DNA Databases in the World ..................................................................................................... 225
8.2.1. CODIS Database – United States of America ............................................................................... 225
8.2.2 NDNAD Database – England ....................................................................................................... 226
8.2.3. Application of DNA Analysis in Germany ................................................................................... 227
8.2.4. European Exchange of DNA Data ................................................................................................ 228
8.2.5 INTERPOL’s Global DNA Gateway ............................................................................................. 228
8.2.6 Y Chromosome Databases ............................................................................................................. 229
8.3 Current Situation in the Region ................................................................................................................. 229

�Chapter 9
FORENSIC DNA ANALYSIS OF PLANT AND ANIMAL
BIOLOGICAL TRACES .......................................................................................................... 231
9.1. Forensic botany ....................................................................................................................................... 234
9.1.1. Fundamentals of the molecular and genetic techniques in plant material analysis ..................... 234
9.1.1.1. Analysis of STR molecular markers on plant traces ............................................................ 234
9.1.2. Analysis of random/unknown markers ......................................................................................... 235
9.1.2.1. Analysis of randomly amplified polymorphic DNA ............................................................ 235
9.1.2.2. Amplified fragment length polymorphisms ........................................................................ 236
9.1.2.3. Species identification .......................................................................................................... 237
9.1.3. Palynology and Mycology ............................................................................................................ 238
9.2. Fundamentals of animal forensic DNA analysis ..................................................................................... 238
9.2.1. Forensic entomology .................................................................................................................... 238
9.2.1.1. Analysis of human DNA isolated from insects .................................................................... 240
9.2.1.2. Ribonucleic acid analysis (RNA analysis) ........................................................................... 240
9.2.2. Forensic DNA analysis of vertebrates ........................................................................................... 240
9.2.2.1. Analysis of animal nuclear DNA ......................................................................................... 240
9.2.2.2. Analysis of animal mitochondrial DNA .............................................................................. 242
9.2.2. Forensic DNA analysis in the control of food products ............................................................... 242

Chapter 10
FOOD FORENSICS ..................................................................................................................... 245
10.1. Food Fraud ............................................................................................................................................ 248
10.2. Definitions ............................................................................................................................................. 249
10.3. Methods used in food forensics ............................................................................................................. 249
10.3.1. DNA analysis in food forensics .................................................................................................. 250
10.3.2. Applications of DNA analysis in food forensics ........................................................................ 250
10.3.2.1. Identification of meat and fish origin in food products ...................................................... 250
10.3.2.2. Tracing of botanical origin and adulteration identification in basmati rice ........................ 253
10.3.2.3. The provenance of olive oil ............................................................................................... 253
10.3.2.4. Durum wheat pasta adulteration ........................................................................................ 253
10.3.3. Forensic toxicology .................................................................................................................... 254
10.3.4. Genetically modified organisms (GMO) .................................................................................... 254
10.3.5. Trace elements ............................................................................................................................ 255
10.4. Conclusions ........................................................................................................................................... 255

Chapter 11
MICROBIOMES AS TOOLS IN HUMAN IDENTIFICATION ................ 259
11.1. Microbial forensics – an introduction ..................................................................................................... 262
11.2. Human microbial „ID Cards“ ................................................................................................................ 263
11.3. Abundance of microbial species can be individualized ......................................................................... 264

�Contents
11.4. 16S rDNA as microbial signature ........................................................................................................... 266
11.5. Metagenomics reveals complex microbial information ........................................................................ 267

Chapter 12
SUPPLEMENT: SUGGESTED PROCEDURES FOR
COLLECTION AND LABELING OF BIOLOGICAL TRACES
FOR DNA ANALYSIS ................................................................................................................. 271
12.1. Collection of blood samples ................................................................................................................... 274
12.1.1. Collection of wet blood samples from objects that cannot be delivered for analysis .................. 274
12.1.2. Collection of wet blood samples from objects that can be delivered for analysis ...................... 274
12.1.3. Collection of dry blood samples from objects that cannot be delivered for analysis ................. 275
12.1.4. Collection of dry blood samples from objects that can be delivered for analysis ...................... 276
12.1.5. Blood samples found on wet or moist clothes and shoes ........................................................... 276
12.2. Collection of sperm samples .................................................................................................................. 276
12.2.1. Time period in which it is necessary to collect a sperm sample ................................................ 276
12.2.2. Collection of wet (fresh) sperm samples from objects that cannot be delivered
for analysis .................................................................................................................................. 277
12.2.3. Collection of wet (fresh) sperm traces from objects which can be delivered for analysis ........... 277
12.2.4. Collection of dry sperm samples from objects that cannot be delivered for analysis ................ 278
12.2.5. Collection of dry sperm samples from objects that can be delivered for analysis ..................... 278
12.2.6. Collection of sperm samples found inside the victim’s body (vaginal and anal smear) ............. 278
12.2.7. Collection of sperm samples found on the victim’s body ............................................................ 279
12.2.8. Collection of Sperm Samples from the Mouth (Buccal Swab) .................................................. 279
12.2.9. Early Paternity Dispute Testing .................................................................................................. 279
12.3. Collection of Saliva Samples ................................................................................................................ 279
12.3.1. Collection of wet (fresh) saliva traces from objects that cannot be delivered for analysis .......... 279
12.3.2. Collection of wet (fresh) saliva traces from objects that can be delivered for analysis ............. 280
12.3.3. Collection of dry saliva traces from objects that cannot be delivered for analysis .................... 280
12.3.4. Collection of dry saliva traces from objects that can be delivered for analysis ......................... 280
12.3.5. Collection of Saliva Samples from the Body ............................................................................. 281
12.4. Collection of Hair Samples .................................................................................................................... 281
12.4.1. Collection of Hair Samples Found at the Crime Scene .............................................................. 281
12.4.2. Collection of Hair Samples from the Victim’s Body ................................................................... 281
12.4.3. Collection of Hair Samples from the Body of a Victim Potentially Containing
Sperm or Blood Traces ............................................................................................................... 282
12.5. Collection of Biological Samples under the Nails and from Feces ....................................................... 282
12.6. Collection of Reference Biological Samples .......................................................................................... 282
12.6.1. Collection of Reference Buccal Mucous Membrane Samples .................................................... 283
12.6.2. Collection of Reference Blood Samples ..................................................................................... 283
12.6.3. Collection of Skeletal Remains .................................................................................................. 283
12.6.4. Collection of Personal Objects for Use as Reference Samples .................................................. 284
Index ............................................................................................................................................................... 285

�FOREWORD
The science of the 21st century has not given up on its rapid development. The pace
of evident progress in certain scientific disciplines, especially those relying on applied
genetics, does not allow for a breakthrough in the collection, sorting and presentation
of the latest achievements made in hundreds of laboratories around the world. Continuous education of scientists, professors, experts, and users of scientific achievements
has never been this prominent and observable.
After a brief analysis of the development of forensic genetics in the past five years, we
have decided that it would be wise to approach the complementation of existing material available with, as we then thought, “some new information”. But when we included
everything we wanted to add onto the previous edition, we found that the new facts, hypotheses and models have been generated, as well as a promising direction for potential
development established. Soon upon this realization, we had nothing left to do but to,
significantly influenced by young and enthusiastic associates, “roll up our sleeves” and
prepare a new textbook. As a result, this book was created, which at the moment of its
creation is probably the only existing edition that includes the most up-to-date information, especially related to the new multiplex STR systems, next-generation sequencing
platforms and lineage markers, as well as new approaches in forensic DNA analysis in
general. Two completely new chapters have been prepared, including the topics of food
forensics and microbiology in forensic investigations. We are especially proud of the
last chapter of this book that gives brief, understandable and highly applicable guidelines for proper sample handling, collection and storage, and overall model of behavior
at the crime scene.
As in the previous editions of this material, we tried to present the basic molecular
biological, biochemical, statistical and technological knowledge, and other principles
that must be known in order to comprehend the application of fundamental scientific
knowledge in forensic genetics. Also, we aimed at adding everything that is important
into this book, and also what is written within the best books of the world, and everything that we have learnt from our practical work in the past decade. By preparing this
edition in English language, we have thought of potential international readers of our
book and tried our best to make this text as accessible worldwide as possible.

Damir Marjanović
Dragan Primorac
Serkan Doğan
Sarajevo/Zagreb, Summer of 2018

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                <text>Abstract: The current research is an attempt to offer new insights into the association between hiring high-quality audit firms and corporate debt maturity structure.  To this end, 94 firms listed on the Tehran Stock Exchange were scrutinized for the period 2011-2015. To test the research hypotheses, multiple regression and data panel were employed. The findings confirm that a high-quality audit firm can enhance the debt maturity. The results of testing the research hypothesis point to a significant correlation between the hiring of a high-quality audit firm and corporate debt maturity structure, in that short-term debt and quality audit are two alternative mechanisms used to mitigate information asymmetry and monitor managerial behavior. Therefore, in firms audited by high-quality audit firms, due to the effective monitoring imposed by auditors on debt convent, creditors experience information asymmetry and less agency costs, thereby desiring to extend the debt maturity. The findings of current study not only fill existing gaps in the field, but also contribute to decision-making practices in stock exchange.</text>
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                    <text>Tourist Potential as a Factor of Rural Territories Development: Experience of
the Russian Federation Regions
Polina Ananchenkova
Academy of labour and social relations
Russia
ananchenkova@yandex.ru
Abstract: World experience shows that in Russia and other countries, the development of rural tourism
can be a very effective activity aimed at environmental, economic, and social development.
Today, about 20000 villages in Russia are on the verge of extinction. Consequently, rural tourism can
and should become the very “locomotive” that will really pull out and revive the rural areas of Russia.
The development of rural tourism is named among the priority area provided for in the “Concept of
Sustainable Development of Rural Territories of the Russian Federation for the period up to 2020”,
approved by Order of the Government of Russia in 2010.
Expert assessments based on studies conducted in the regions of the Russian Federation show that the
approximate level of income from rural tourism activity per administrative region of the constituent
entity of Russia may be about 30 million rubles (500 thousand US dollars) per year. In the case of the
implementation of an integrated approach involving the joint development of agricultural activities and
rural tourism, the effect can double – up to 50-60 billion rubles per year.
Rural tourism in Russia is a new socio-economic phenomenon oriented to the use of natural, cultural,
historical resources of rural areas to create and supply a tourist product to a wide range of people. In
this regard, it should be thought that the strategic priorities of the national policy aimed at stimulating
tourism in rural areas should be the concentration of tourist resources and creation of destinations that
include backbone tourist centers with high potential for the formation of rural tours, and territories
adjacent to the borders. At the same time, the activation of rural tourism will allow developing territories
that do not have promising industrial and agricultural organizations, and thereby improve the socioeconomic situation of the country as a whole.
Keywords: rural territories, development, tourism, Russian Federation
JEL Classification: Z320, R190, Q010

Introduction
World experience shows that in Russia and other countries, the development of rural tourism can
be a very effective activity aimed at environmental, economic, and social development. At the
end of XX and beginning of XXI century in the world a great interest to rural tourism has arisen,
and different countries have excellent examples of organization and promotion of this type of
tourism. At this, each country thanks to natural landscapes, traditions of agriculture and cattle
breeding, specifics of cuisine, rural culture, architectural appearance and vernacular legacy of
villages create own national model of rural tourism development. Rural tourism is slowly
56

�becoming popular, fashionable and recreation in countryside already takes second place after
seaside vacation. Russia does not remain aside from modern trends in this area. Today, about
20,000 villages in Russia are on the verge of extinction. Consequently, rural tourism can and
should become the very “locomotive” that will really pull out and revive the rural areas of
Russia.
Subjects of rural tourism services provision
According to data of monitoring of rural tourism development in regions of the Russian
Federation, subjects of rural tourism services provision, in general, are:
- small businesses providing accommodation, catering, and excursion services;
- Peasant Farm Enterprises, Household Farms, farmers;
- food processing enterprises;
- administration of Specially Protected Natural Reservations (SPNR);
- institutions of culture and sports in rural areas, in some cases, schools;
- religious sites;
- rural residents and summer visitors.
Subjects of services provision have one thing in common, i.e. all of them are located at rural
area, but due to organizational-legal stats and different departmental affiliation there is a certain
discrepancy in interests.
List of services provided to tourists nowadays in almost each region is rather diverse, and
depends on demand as well as on initiative and wishes of subjects providing services.
Approximate range of services is as follows:
- services of temporary accommodation in guest rooms, rural guest houses, houses, inns, etc.;
- catering and tasting services;
- agro-tourist services (participation in agricultural operations: haymaking, shearing, milking,
etc.);
- agro-tourist services (gathering of mushrooms, berries, vegetables, wild plants, etc.);
- sale of agricultural products, meat, dairy products, wild plants, crafts, etc.;
- riding horses, deer, camels, dogs, agricultural machinery;
- services of fishing, hunting;
- rental services, baths, bakeries, smokehouses, parking lots, etc.
- cultural and educational facilities (rural Museum, Club, library);
- training and teaching handicrafts and trades;
- participation in event activities and religious holidays;
- other.
Despite wide range of services to be provided within rural tourism subjects’ activities are
focused on three directions:
57

�At first, services of temporary accommodation in guest rooms, rural guest houses, houses, inns,
etc. One of the first regions of Russia to have achieved positive results in reception of tourists in
private (rural) houses with “bed &amp; breakfast” services as well as in teaching local residents the
basics of tourism organization is the Republic of Karelia (http://www.ticrk.ru). Rural estates’
owners in Karelia were supported within frames of implementation of international project,
Tacis, “Development of rural tourism on basis of rational use of natural and cultural resources”
main result of which according to experts was establishment of Association of Rural Estates’
Owners “Estate” for coordination and support of activities of rural houses’ owners.
Second, tourist and tour services on basis of agricultural enterprises, farms, fishing facilities,
facilities of food processing industry, and etc. It should be noted that National Standard of the
Russian Federation GOST R 50681-2010 “Tourist Services. Design of Tourist Services”
determines co-providers of tourist services:
Organizations and enterprises of tourist industry as well as private entrepreneurs rendering
certain services (accommodation, catering, transport entities, exhibition complexes, museums,
and etc.) under contracts concluded with tour operators at formation of tourist product.
Obviously, objects of agricultural sector providing tourist and tour services refer to this list to
the similar ones.
In many Russian regions tours to special agricultural farms/agricultural production facilities
become rather popular, during which the visitor can get acquainted not only with production
process but also can try and purchase ready products, can learn some recipes, etc. (cheese
makers, wine makers, smokehouses, dairies, mushroom farms, etc.). Level of agriculture
development in regions attracts attention of both farmers and manufacturers of agricultural
products, and people interested in agriculture of the region.
Third, tourist and tour services rendered by rural cultural institutions (libraries, museums,
cultural and recreational institutions), whereby we are talking not only about tours, acquaintance
with life and traditions, visiting religious sites, eves, but also about teaching handicrafts and
trades, singing and dancing skills, and etc.
In 2014, for the first, in federal budget there was allocated over three billion Rubles for
renovation of material base of culture in villages and small towns. Mobile multi-functional
cultural centers have been provided for rural area. Since 2016 till 2020 Federal Target Program
“Sustainable Development of Rural Territories” where Ministry of Culture of the Russian
Federation is a state customer, for reconstruction of operating rural institutions of culture
provides for 2.8 billion Rubles from Federal Budget. Today, outside culture it is impossible to
ensure better quality and variety of services of cultural, -educational, ethnographic, rural, event,
and social tourism. These are exactly the types of tourism that significantly contribute to
education and moral improvement of society, civil patriotism and harmonization of inter-ethnic
relations, development of tolerance and respect for cultures of different peoples.

58

�State support of rural tourism in the Russian Federation
Nowadays, a great attention in the Russian Federation is drawn to state support of tourism
development in general and rural tourism in particular.
Strategy of Tourism Development in the Russian Federation until 2020 under Federal Target
Program “Development of Domestic and Inbound Tourism in the Russian Federation (20112018)” defines rural and ecological tourism as most promising for majority of regions of Russia.
This involves a range of issues, i.e. creation of tourist product, rational use of natural and
cultural heritage of the region, interests of local economy on basis of interaction and mutual
benefits, full support of driving force of rural tourism, entrepreneurship, involvement of
community in tourism development, linking interests of businesses and residents on basis of
mutually beneficial cooperation. Today, in many Russian regions priority of rural tourism
development has been confirmed by real actions and quite tangible results. And, and in light of
modern realities in terms of imposition of sanctions, stimulation of interest in products and
services of domestic tourism will increase business and investment activity in field of rural
tourism.
In 2014, the Ministry of Culture of the Russian Federation and the Federal Agency for Tourism
hosted a number of major events in field of rural tourism. These are international forum
“Agrotourism in Russia” held at the Republic of Buryatia, Russian Conference on Conservation
of Village Culture and Rural Tourism Development in Lipetsk, tourist forum “Rural Tourism in
Russia” in the Orenburg region. Within each event there were discussed current trends and
experiences of development of rural tourism in regions of the Russian Federation, especially
organization of tourist activity in rural areas and problems of legislative, infrastructure,
financial, personnel, informational support of development of rural tourism. Special attention
was given to measures of state support for rural tourism development at federal, regional and
municipal levels. Of vast interest there was experience of self-organization and coordination of
agro-tourist activities within such projects as the “Association of Most Beautiful Villages of
Russia”, and “Peasant Hospitality”. At presentation venues of Forums considerable part of space
has been showcasing best regional projects in chosen areas of rural tourism development
included in collection of materials reflecting best practices of subjects of the Russian Federation
on development of rural tourism.
Concept of sustainable development of rural areas of the Russian Federation for a period until
2020, and Federal Target Program “Sustainable Development of Rural Territories of the Russian
Federation for a Period till 2020” define objectives of stimulating increase in jobs in nonagricultural fields of activity in all possible organizational forms, especially, in field of
recreational and environment protection activities, agro- and environmental tourism, rural hotel
business. To improve cultural services for rural population, preservation and development of
cultural heritage, and enhance creative capacity of rural inhabitants there was determined an
objective of rebuilding, having cultural and historical significance farmsteads and other
59

�architectural and natural monuments, creation of museums, manor-ethnographic complexes and
other infrastructure of rural tourism as well as other activities.
By Decree of the Government of the Russian Federation as of February 2, 2015 No. 151-r there
was adopted a strategy for sustainable development of rural territories for a period till 2030
aimed at creation of conditions for sustained improvements in quality and standard of living of
rural population through benefits of rural lifestyle. Strategy states that rural tourism is one of
major directions of non-agricultural types of activity as well as has important socio-economical
functions of creation of attractive jobs including for rural youth and women; on development of
rural territories; on complex use of natural and cultural potentials of rural areas. Most important
for subjects of agricultural activities providing tourist services, for tourist companies organizing
tours to rural areas, for regional and local governments is a need to implement measures aimed
at formation of agro-tourist clusters; at holding educational events (advanced qualification
courses, educational workshops, master-classes, and trainings) for owner of rural guest houses,
representative of farms, private entrepreneurs, rural residents engaged in organization and
provision of tourist services in rural areas; media coverage of best practice and most successful
projects on development of rural tourism.
Analysis of measures of state support for rural tourism in regions has shown that more used
measures are provision of subsidies and grants (for repair and reconstruction of rural guest
houses, landscaping; participation in exhibitions; promotion; contests; training). Recipients of
subsidies and grants are small enterprises (owners of guest houses, manors, ethnicity complexes,
and farms in rare cases). In majority of regions state support of rural tourism is provided within
frames of state programs on development of culture and tourism, development of agriculture,
support for small businesses. Exception is Belgorod Region, where program of “Development of
rural tourism in the Belgorod Region for 2011-2015” is being implemented.
The main problems of rural tourism development in the Russian Federation

Analysis of materials of conferences, forums, round tables on rural tourism determined key
problems of rural tourism development in regions of the Russian Federation which hinder
effective and dynamic development of this direction of rural population occupancy.
1) Existing federal regulatory-legal acts governing activities in field of tourism lack the
concept of rural tourism. Regional practice for identification of discussed type of tourist
activity widely apply following concepts: “agro-tourism”, “green tourism”, “farm
tourism”, “village tourism”, and etc. However, nowadays, all those regulating this field
of activity and those engaged in this type of activity, as well as supervisory authorities
and consumers have to use single legal definition.
2) Currently in regions main means for accommodation in rural areas are "guest houses"
whereas paragraph 3.3 of the national standard of the Russian Federation GOST R
51185-2008 “Tourist services. Accommodation. General requirements” approved by the
60

�3)

4)

5)

6)

7)

Decree of Rostechregulirovanie as of 18.12.2008 No. 518-st specifies only “guest rooms,
chalets, bungalows, caravans”.
Significant problem for development of rural tourism is underdeveloped and in some
municipalities missing transport and engineering infrastructure (roads, electricity, water
networks, sewage treatment plants, bank protections, etc.), which is an obstacle to private
investments in tourism industry. Lack of telephone communication, Internet is often an
insurmountable obstacle for both, tourists and service providers. But above all, absence
of roads to rural tourism sites which include guest houses, and farms.
In most regions, one of the most important motivations for recreation at countryside is
visiting sites of cultural and historical heritage, religious sites, ethnographic and museum
complexes, old houses, castles, estates, etc.
Tourism allows for drawing attention to unique historical and cultural values, discoveries
of earlier unknown or unfairly forgotten historical settlements, sites and names.
Experts note inadequate involvement of values of the territory in tour programs.
In many Russian regions growing demand for rural tourism services enhanced
investment activities in reconstruction and construction of guest houses, small hotels,
manors, ethnicity complexes, catering, rural markets, trading pavilions, etc. Today it is
quite difficult to obtain available loans for implementation of these projects in rural
areas. According to experts, it is necessary to provide for small businesses in field of
rural tourism concessional borrowings, as well as development of methodical
recommendations and providing special advice for preparation and implementation of
business project.
Significant problem is misunderstanding and sometimes ignorance by rural entrepreneurs
of need to use market-based instruments for promotion of rural tourism services. In many
regions there are formed information databases of rural tourism sites, and first of all, of
accommodation facilities.
According to experts, there is a need in consolidated actions of rural tourism businesses
and greater cooperation on part of authorities on organization of study tours,
participation in exhibitions, advertising, website creation, etc. Experts recommend to
regularly fill the portal www.naselo.ru, the first Russian social network designed to
increase tourist flow to the countryside.
Serious and long-term challenge is staffing of rural tourism which shall be addressed
comprehensively. To avoid mistakes and disappointments, it is necessary to know
organization of tourist services, hotel and restaurant business, marketing rules,
calculation of costs, cost recovery and funding strategy. In some regions of the Russian
Federation (Altai Region, the Republic of Buryatia, the Republic of Karelia, Pskov,
Ryazan, Sverdlovsk regions, etc.) there is ongoing large and coherent work on
organization of training courses, development of methodological recommendations for
development of rural tourism. With purpose of informational, legal and financial support
it is proposed to establish informational-methodological centers for owners of rural
houses and providers of accompanying services for rural tourism.

61

�8) Insufficient level of coordination of activities in field of rural tourism, despite increased
attention to creation of associations of rural tourism. At the federal level, there are such
associations as the “Association for promotion of rural tourism”, “National association of
rural tourism entities” which pay especial attention to issues of development of
regulatory base for rural tourism; classification of objects of rural (agro) tourism, first of
all, rural guest houses; development of mechanisms for address support of rural tourism
subjects; informational and consultancy support of business entities; increase of role of
local governments in development of rural tourism.
Experts of Associations are confident that success of rural tourism development largely
depends on local governments, their ability to coordinate planning and management of
tourism development, encourage participation of local people in arrangement of tourist
services, advise and help those people who want to establish business, to support
development of rural culture.
Conclusion
At the end of XX and beginning of XXI century in the world a great interest to rural tourism has
arisen, and different countries have excellent examples of organization and promotion of this
type of tourism. At this, each country thanks to natural landscapes, traditions of agriculture and
cattle breeding, specifics of cuisine, rural culture, architectural appearance and vernacular legacy
of villages create own national model of rural tourism development. Russia does not remain
aside from modern trends in this area. Rural tourism is slowly becoming popular, fashionable
and recreation in countryside already takes second place after seaside vacation.

References
Kamilov M., Kamilova P., Kamilova, Z. (2016). Rural tourism as a factor of sustainable
development of territories. Russian Agriculture Economy, 12, 39-54.
Novichkov N., Novikov V., Novichkova N. (2014). Development of tourism at rural territories
of Russia in context of number of social-economic trends. Russian Agriculture Economy, 3,
7-14.
Shimova O.S. (2012). Basics of sustainable tourism. - Moscow: INFRA-M.
Russia in figures. 2012: Brief statistical collection/Rosstat. - M., 2012
Russia in figures. Annual Report for 2015 (2016). Moscow: Federal state statistics service.
Main indicators of agriculture in Russia. Annual Report for 2015 (2016). Moscow: Federal state
statistics service.
Leontiev J., Timoshenkova O. (2013). Regional tourist destination and its social-economic
development. Retrieved from: http://business-inform.net/pdf/2013/6_0/165_170.pdf

62

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                <text>Abstract: World experience shows that in Russia and other countries, the development of rural tourism  can be a very effective activity aimed at environmental, economic, and social development.  Today, about 20000 villages in Russia are on the verge of extinction. Consequently, rural tourism can  and should become the very “locomotive” that will really pull out and revive the rural areas of Russia.  The development of rural tourism is named among the priority area provided for in the “Concept of  Sustainable Development of Rural Territories of the Russian Federation for the period up to 2020”,  approved by Order of the Government of Russia in 2010.    Expert assessments based on studies conducted in the regions of the Russian Federation show that the  approximate level of income from rural tourism activity per administrative region of the constituent  entity of Russia may be about 30 million rubles (500 thousand US dollars) per year. In the case of the  implementation of an integrated approach involving the joint development of agricultural activities and  rural tourism, the effect can double – up to 50-60 billion rubles per year.    Rural tourism in Russia is a new socio-economic phenomenon oriented to the use of natural, cultural,  historical resources of rural areas to create and supply a tourist product to a wide range of people. In  this regard, it should be thought that the strategic priorities of the national policy aimed at stimulating  tourism in rural areas should be the concentration of tourist resources and creation of destinations that  include backbone tourist centers with high potential for the formation of rural tours, and territories  adjacent to the borders. At the same time, the activation of rural tourism will allow developing territories  that do not have promising industrial and agricultural organizations, and thereby improve the socioeconomic  situation  of  the  country  as  a  whole.        Keywords:  rural  territories,  development,  tourism,  Russian  Federation</text>
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                    <text>Quality of Education and Intellectual Capital: Analysis of the Competitive
Position of Universities
Dino Arnaut
Faculty of Economics, University of Zenica
Bosnia and Herzegovina
arnaut.dino@gmail.com
Abstract: Intellectual capital opened the way for research into this area, which forms the basis of the
new knowledge economy. The development of intellectual capital has a growing impact on the economic
and social processes. Intellectual capital is now even more important than tangible assets. If a country
wants to develop and become economically strong and enlightened it needs to build a modern and
flexible and well suited and efficient education system that is ready and able to responses to all the
global technological and social changes, as well as the local current social needs. To achieve this there
is a need to establish institutional cooperation between universities, governments, research institutions.
Education has a double meaning that is economic and social. It represents a means to improve economic
standards as well to spread spiritual perspective and improvement of own intellectual and emotional life.
Sociologically it is established that education is beneficial both for the individual and for society.
Therefore, it not only contributes to higher profits for the individual, but also its better social status and
reputation of the company, financial and social security, development of identity and self-confidence,
self-esteem development and personal satisfaction, better understanding of the political situation and
greater social engagement and cohesion, respect for social norms, reducing stereotypes and prejudice,
improving gender relations and better education of children, development of tolerance and ethical
behaviour, aspirations towards healthy living, and better physical and mental health. Investments in
education are an important factor for competitiveness, growth and development of a country. Education
has a key role in improving the human capital and the development of a knowledge based society. It
contributes to the unification of life chances, personality development in the spirit of liberty, intellectual
development and spiritual and cultural richness. The aim of this paper is to investigate connection
between service quality and competitive position of Universities in Bosnia and Herzegovina to help
developing new and improved academic programs that will contribute development of future strategies
based on intellectual capital.
Keywords: Intellectual Capital, Quality of Education, Education System, Service Quality, Bosnia and
Herzegovina
JEL Classification: O34, I2, M3

Introduction
Over the last few decades, the quality of service has been gained and takes tremendous attention
from both managers and academics due to their significant impact on business results, cost
reduction, customer satisfaction and loyalty, as well as profitability. Therefore, quality is
increasingly seen as an investment for a company or institution, where efforts to improve and
improve it result in an increase in the number of customers (consumers), as well as increase the
volume of purchases from existing customers, which also leads to growth of company profit.
84

�To gain competitive advantage among other higher education institutions, universities require
greater focus on service quality. Therefore, universities want and try to examine their current
strategic positions by evaluating existing services and adapting to consumer perceptions to
improve or gain their leadership position.
Intellectual capital (IC) opened the way for research into this area, which forms the basis of the
new knowledge economy. The development of intellectual capital has a growing impact on the
economic and social processes and now it is even more important than tangible assets. If a
country wants to develop and become economically strong and enlightened it needs to build a
modern and flexible and well suited and efficient education system that is ready and able to
responses to all the global technological and social changes, as well as the local current social
needs.
To achieve this there is a need to establish institutional cooperation between universities,
governments, research institutions. Therefore, just defining and measuring service quality at
universities can serve as an initial step towards more orientated and friendly education services
for students, as well as improving the overall provision of services in educational institutions.
This provides room for the establishment of clear consumer-oriented standards and the
establishment of benchmarks for quality service comparison both in public and private
universities. Education has a double meaning that is economic and social. It represents a means
to improve economic standards as well to spread spiritual perspective and improvement of own
intellectual and emotional life.
Literature review
European higher education and research organizations have undergone a deep transformation
process over the past decades. This process can be analysed by considering two parallel
processes.
The first process is the theoretical insight that provides two perspectives of evolutionary
significance. These are the so-called two knowledge production methods (Gibbons et al., 1994)
and the triple Helix model (Etzkowitz and Leydesdorff, 1996). Both perspectives emphasize the
emergence of a new paradigm of knowledge production that is defined by transdisciplinary and
research-oriented solutions. In this scenario, the relationship between university, industry and
government becomes more dynamic and mutually dependable and conditioned, thus contributing
to the creation of hybrid organizations, the creation of alliances between universities and firms,
and the creation of a trilateral network and other forms of cooperation that enhance the quality of
education. Therefore, universities themselves are interacting with various alternative knowledge
producers (Gibbons, 1998, p.1). This framework is most commonly accepted in professional
literature and has become crucial for understanding the role of universities and their connection
with other actors in the current economy (Mowery and Sampat, 2004).

85

�The second process is an ever-growing interest in higher education institutions and intensive
discussions about the role they play in the paradigm shift. This process is primarily represented
by the European Commission's (2006) policy actions and the resulting collective process in
some institutions such as the European Association of Universities (EUAs), the European
Association of Managers and Research Administrators (EARMA), as well as individual groups
of experts, such as the group responsible for reporting on intellectual capital to increase research,
development and innovation in small and medium-sized enterprises (RICARDIS report).
Adaptation of management and reporting of IC in companies to other types of organizations
developed in two different ways. First, it primarily deals with the assessment of intangible assets
aggregated into the mezo (communities, industry, etc.) and at the macro level (cities, regions and
nations). Thus, the World Bank has organized various conferences on this issue in the period
2005-2007. (Chatzkel, 2006). Since 1999, efforts have been made to measure the state-level IC,
starting with Sweden (Rembe, 1999), Israel (Pasher, 1999) and the Arab region (Bontis, 2004),
and so on.
Another way suggests the use of IC framework at the micro level for public institutions. Some
papers involved in this group are based on the principles of new public management. These
principles have been used by governments since the 1980s to improve the efficiency of the
public sector and the quality of their services, through the decentralization process and the
application of competition, by treating consumers of public services as consumers. In this way,
governments give a certain institution more autonomy to fulfil its goals and reward the effect
(Borins, 1995), which requires measurement and reporting mechanisms, in accordance with
appropriate revision rules. This phenomenon was initially seen as a problem for developed
countries, particularly Anglo-Saxon, with best case studies in Great Britain, Australia and New
Zealand (Barzelay 2001, Guthrie et al., 2004). USA, Canada and, to a lesser extent, some
European countries have caught their attention (Borins 2002, Guthrie et al., 2004), and the
principles are provisionally applied in some African developing countries (Larbi, 1999).
This paper shares the same opinion and agrees with the views of Mouritsen et al. (2005) and
Leitner et al. (2005) in the sense that the IC framework is a valid attempt to meet the new
demands of public institutions and that the IC report is useful tool for internal and external
purposes. The IC report can help identify structural and personal strengths and weaknesses. It
discovers the current state of the various university missions and can be used as a control and
monitoring instrument (Altenburger and Schaffhauser-Linzatti, 2006).
The purpose of the ICU report, which is also an integral part of the OEU project, is to make
recommendations for publishing university research information. In accordance with the
recommendations of the European Commission (2006), the report presents a logical shift from
management and internal strategy, based on the design of the vision and objectives of the
institution, to the publication of indicators considering the previous guidelines valid for
companies (Meritum Protect, 2002), and for the universities (Leitner and Warden, 2004).
86

�The indicators have classified the next well-known taxonomy into three categories of capital,
namely human, organizational and relational capital. Within each of these categories, each title
monitors the strategic issues defined in the OEU guide. The guide itself suggests that indicators
are expressed both in absolute and relative terms to make easy comparisons easier.
University rankings
Over the last ten years there has been an increasing interest in ranking the university. The annual
ranking of world universities is published by many, starting with QS for the Times Higher
Education Supplement, the Shanghai Jiao Tong University, the Higher Education and
Accreditation Council of Taiwan, and Cybermetrics Lab in CSIC.
The Academic Ranking of World Universities (ARWU) is being published each year by the
Shanghai Jiao Tong University of Higher Education Institute. This is the first level of ranking
with the intent of worldwide coverage based on the academic or research effectiveness of the
university. Its indicators include alumni and staff that received Nobel or similar prestigious
awards, highly quoted researchers in popular research fields, articles published in selected top
magazines, indexed index articles by Thomson-ISI, and performance by academics.
The Web Ranking of World Universities or Webometrics List is being conducted since 2004
(Aguillo et al, 2008) by Cybermetrics Lab, a research group of the Spanish National Research
Council (CSIC). They use web data downloaded from commercial search engines, including
web pages, rich format documents (pdf, doc, ppt and ps), works indexed by Google Scholar (this
indicator was added in 2006) and many external links as a measure Link visibility or impact.
Table 1: Different emphasis of different university rankings
Orientation to students
US News &amp;

Orientation to Research
Webometrics

Shanghai

Taiwan

Leiden

WR

ARWU

HEEACT

CWTS

THE-QS
WR McLeans
Costs
Opinions
Services

Impact

Scientific
contribution

Web visibility

Prestige

Web presence

Awards

Impact

Excellence

Source: Authors’ own work

The specifics of university rankings are shown in Table 1. As can be seen, certain rankings are
strictly based on research data. Webometrics, on the other hand, has one weakness, and many
universities do not have a strict web policy. This is not such a big deal with the universities in
this research, so this weakness may be neglected.

87

�Compared to the indicators of intellectual capital of higher education institutions and ranking of
universities, we conclude that there are common indicators. Therefore, as part of this research,
the Webometrics list will be used as the rank of success of the tested universities and their
competitiveness on the market of Bosnia and Herzegovina. Their position will be tested and
compared with their perceived quality of service.
Service Quality
There is a lot about service quality in the literature itself. We have many quality definitions as
well as its concept and its different dimensions. Thus, according to Juran (1988), quality is a
convenience for use, that is, to what extent the product successfully serves the purpose of the
user when used. Crosby (1982) argues that quality is in line with requirements. Gronroos (1984)
is one of the first academics to focus on quality of service. According to him, the quality of
service consists of two dimensions, technical quality and functional quality. Technical quality
refers to the outcome, that is to what the customer has received from the service itself and can be
measured in a similar way as the quality assessment of the product. On the other hand,
functional quality refers to the process of evaluating the way of providing services. Image is an
important factor affecting the service quality, and serves as a filter in perceiving quality of
service as favourable, neutral or unfavourable (Gronroos 1984, 2000).
In a sophisticated 1988 study, Parasuraman et al. have reduced the original number of service
quality dimensions from ten to five, claiming that these five dimensions fully cover the domain
of service quality. Thus, the five finals of quality of service, according to Parasuraman et al.
(1985) are:
• Tangibles - the physical dimension of a service, such as state of the building, equipment,
staff appearance, and the like.
• Reliability - the ability to deliver the promised service, reliably, accurately and on time.
• Responsiveness - willingness and willingness to help customers and provide fast service.
• Assurance - knowledge and kindness of employees and their ability to inspire and
stimulate trust and confidence.
• Empathy - attitude, individualized relationship, and attention paid by the company
towards its customers (customers).
Parasuraman et al. (1988) have also developed a service quality assessment tool called
SERVQUAL, which is a multifaceted scale with good reliability and validity. The scale consists
of two parts evaluating the quality of the service. The first part is a section of expectation that
contains 22 statements to measure the expectation of quality of service by the consumer
(customer). The second section is a perception section that contains the appropriate set of 22
statements to measure how users perceive (experience) the quality of the service. In these
sections, for expectations and perceptions, use the same phrases with the difference that one asks
about what the respondent expects from an excellent service provider, and the other asks about
the actual, perceived, service provided. Consumers give their grades on the expectations and
88

�perceptions of the quality of services on a seven-point Likert scale, which range from
completely disagree (1) to completely agree (7).
The quality of services is calculated by the difference between estimated expectations and
perceptions, that is, the gap between them. Parasuraman et al. (1994) found that the
SERVQUAL scale is a very useful starting point for measuring the quality of services.
Three contrastive approaches to quality measurement can be classified within the education. The
first approach adjusts the SERVQUAL instrument (Rigotti and Pitt, 1992, Cuthbert, 1996a,
1996b, Owlia and Aspinall, 1996, Oldfield and Baron, 2000, O'Neill and Palmer, 2001). The
other uses methods for evaluating the quality of teaching and learning (Entwistle and Tait, 1990;
Ramsden, 1991; Marsh and Roche, 1993), while the third uses methods for assessing the quality
of overall student experience (Harvey et al., 1992, Roberts and Higgins, 1992; Hill, 1995;
Aldridge and Rowley, 1998; Gaell, 2000; Watson et al., 2002; Wiers-Jenssen et al., 2002).
In the studies in which SERVQUAL is applied, it is necessary to modify the questionnaire, and
there is no consensus on the dimensions of service quality and the importance of each dimension
in the context of higher education. However, studies support the importance and reliability of
this methodology within the measurement of the quality of higher education. Tan (1986)
conducted a review of the methods used to assess the quality of teaching in higher education in
the USA area back in 1986, in which three types of studies are differentiated, namely reputations
involving the evaluation of subjects by experts, objective indicators and quantitative studies.
Methodology
Four universities have been chosen for this study to conduct a study on the quality of services in
higher education. Of these four universities, two are public and two are private. They all offer
programs at bachelor and master level, and three of them also offer doctoral studies (PhD). The
survey sample consists of 388 undergraduate and master students. Data collection was carried
out during 2013.
Since it was difficult to include students from all universities in the territory of Bosnia and
Herzegovina, we selected to include four university students, two public and two private, based
on their ranking according to Webometrics. Universities were selected according to the criteria
of the two best public and private universities in the territory of Bosnia and Herzegovina
according to Webometrics ranking. These were the following universities:
• University of Sarajevo (UNSA).
• University of Zenica (UNZE).
• International Burc University (IBU).
• International University of Sarajevo (IUS).

89

�Using the Sample Size Calculator9, we calculated the desired sample size. This calculator is
presented as a public service survey software by Creative Systems Research. Our target
population is 128.119 students in the territory of Bosnia and Herzegovina. Therefore, with
confidence level of 95% and confidence interval of 5 our calculated needed sample size was 383
students.
Also, the number of distributed polls was equally represented by universities. The research tool
was a structured survey consisting of 54 questions. This instrument is chosen because it gives
researchers the ability to collect data on a variety of factors and thus achieve a larger sample. We
collected 388 fully completed surveys via electronic and printed channels, which allowed us to
reach the planned sample size based on the level of reliability and confidence intervals.
Results
As we have already said, in comparison with the indicators of the intellectual capital of higher
education institutions and ranking of universities we can see that there are common indicators.
Therefore, as part of our research, the Webometrics list will be used as the rank-list of successful
universities tested and their competitiveness on the market of Bosnia and Herzegovina.
The university's position is compared to their overall perceived quality of service in all
dimensions (Table 2).
As can be seen in Table 3, ranking according to the Webometrics ranking of the University at
the level of Bosnia and Herzegovina corresponds to the rankings obtained according to the
overall perceived quality of service of the mentioned universities.
Table 2: Total mean value of perceived service
Mean
UNSA

UNZE

IBU

IUS

Total

Tangibles

4.05

3.86

3.83

3.83

3.89

Reliability

3.62

3.51

3.48

3.47

3.52

Responsiveness

4.43

4.25

4.25

4.19

4.28

Assurance

4.02

3.90

3.82

3.74

3.87

Empathy

3.84

3.72

3.60

3.56

3.68

Service quality

4.21

3.85

3.85

3.81

3.93

Total mean

4.03

3.85

3.81

3.77

Source: Authors’ own work
9

The Sample Size calculator can be used and found at http://www.surveysystem.com/sscalc.htm#one

90

�We can conclude that the competitive position (ranking of the university) is directly dependent
on the overall quality provided by the given institution. Therefore, we confirm our claim that the
quality of education services directly affects the competitive position of the educational
institution.

Univerzitet

World ranking

Ranking in Bosnia and
Herzegovina

Mean of total perceived
quality of universities

University of Sarajevo
(UNSA)

1859

1

4.03

University of Zenica
(UNZE)

3531

2

3.85

International Burch
University (IBU)

7400

8

3.81

International University
of Sarajevo (IUS)

7912

9

3.77

It is also noteworthy that the greatest difference in the mean values of total perceived quality is
precisely between the University of Sarajevo, while remaining at approximately the same
average values. It also contributes to the high position of the University of Sarajevo at the
Webometrics rankings.
Conclusions
The research results obtained support the previous quality service studies conducted by various
researchers, and concluded that these five dimensions represent high quality services. This
research serves as an addition to other published research to demonstrate that this model is
applicable to a wide range of services, including the higher education sector.
Ranking according to the Webometrics ranking of the Universities at the level of Bosnia and
Herzegovina corresponds to the rankings obtained according to the overall perceived quality of
service of the mentioned universities. And we see that the competitive position (ranking of the
university) is directly dependent on the overall quality provided by the given institution.
This study also has several limitations. First, not all universities are involved in this study so that
for future studies it would be good to include all private and public universities in the territory of
Bosnia and Herzegovina and to include research institutes. In this way, the sample would be
even more representative and would increase the validity and validity of the research results.
Secondly, this research is necessary to be replicated by other researchers to further determine
and confirm that the modified SERVQUAL scale used in higher education services has its
relevance and validity.

91

�The basic feature of today's market is the vast number of competitors that are constantly
struggling for a limited number of users. Therefore, service companies are increasingly adopting
customer relationship management concepts, especially due to constant user-specific, individualuser-specific access. Higher education institutions, as well as service providers, have the
potential to create an advantage and maintain and develop a long-term relationship between
them, as providers of services, their service users, students, solving their problems and making
them loyal to institution. In this way, in the long run, the clients themselves promote the
institution and in some ways become the walking image of the higher education institution.
There is a need to establish institutional cooperation between universities, governments and
research institutions to achieve those goals. Education represents a means to improve economic
standards as well as a means to spread spiritual perspective and improvement of own intellectual
and emotional life. Sociologically it is established that education is beneficial both for the
individual and for society as a whole.
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94

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                <text>Abstract: Intellectual capital opened the way for research into this area, which forms the basis of the  new knowledge economy. The development of intellectual capital has a growing impact on the economic  and social processes. Intellectual capital is now even more important than tangible assets.  If a country  wants to develop and become economically strong and enlightened it needs to build a modern and  flexible and well suited and efficient education system that is ready and able to responses to all the  global technological and social changes, as well as the local current social needs. To achieve this there  is a need to establish institutional cooperation between universities, governments, research institutions.  Education has a double meaning that is economic and social. It represents a means to improve economic  standards as well to spread spiritual perspective and improvement of own intellectual and emotional life.  Sociologically it is established that education is beneficial both for the individual and for society.  Therefore, it not only contributes to higher profits for the individual, but also its better social status and  reputation of the company, financial and social security, development of identity and self-confidence,  self-esteem development and personal satisfaction, better understanding of the political situation and  greater social engagement and cohesion, respect for social norms, reducing stereotypes and prejudice,  improving gender relations and better education of children, development of tolerance and ethical  behaviour, aspirations towards healthy living, and better physical and mental health. Investments in  education are an important factor for competitiveness, growth and development of a country. Education  has a key role in improving the human capital and the development of a knowledge based society. It  contributes to the unification of life chances, personality development in the spirit of liberty, intellectual  development and spiritual and cultural richness. The aim of this paper is to investigate connection  between service quality and competitive position of Universities in Bosnia and Herzegovina to help  developing new and improved academic programs that will contribute development of future strategies  based on intellectual capital.     Keywords: Intellectual Capital, Quality of Education, Education System, Service Quality, Bosnia and  Herzegovina</text>
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                    <text>Detection of Financial Statement Fraud Using Beneish Model
Elvisa Buljubasic
International Burch University
Bosnia and Herzegovina
elvisa.buljubasic@ibu.edu.ba
Sanel Halilbegovic
International Burch University
Bosnia and Herzegovina
sanel.halilbegovic@ibu.edu.ba

Abstract: One of the greatest challenges faced by auditors is to detect anomalies in financial statement
reporting. Once the anomalies are detected they have to be further investigated by forensic accountants.
However, the practice of forensic accounting has not yet become a reality in Bosnia and Herzegovina. So
the main purpose of the study is to analyze to which degree BH companies are exposed to the financial
statement fraud and with respect to that the need for forensic accountants. The financial statement data
will be collected from BH companies and it will be analyzed using Beneish model. The Beneish model is
the mathematical model that identifies the manipulation of earnings through financial ratios. These
analytical techniques should reveal the unconventional variations in financial statement reporting,
indicating that there is possibility of fraudulent transactions.
Key words: Beneish model, fraudulent reporting, forensic accounting

Introduction
One of the greatest challenges in 21st century faced by companies, institutions and organizations
is financial statement fraud, which is increasing in number and size, what significantly affected
the people’s trust in credibility of financial statements and corporate reports. As stated in the
Report to the Nation on Occupational Fraud and Abuse (2016), published by ACFE, compared to
the other types of occupational fraud (corruption and asset misappropriation), financial statement
fraud is the least frequent (9.6%), however, it is the largest in terms of size of median loss ($975
000).
The financial statement fraud become a global concern, affecting not only large, multinational
companies and organizations, but also small and medium sized enterprises. However, the wellknown corporate scandal cases such as Enron, WorldCom, Tyco and few others are the most
known examples of not only financial statement fraud, but also other types of occupational fraud.
After these corporate scandal cases the issue of auditor’s failure to detect fraud signs or

252

�symptoms was brought to the attention and the role of forensic accountant become irreplaceable
in fraud investigation. In order to be a forensic accountant one has to possess a broad spectrum of
knowledge and skills in different fields such as accounting, auditing, law, psychology,
criminology, etc. Also, the forensic accountant needs to possess outstanding moral and ethical
principles and values.
Technology advancements significantly improved the detection process of frauds and
embezzlements, so today auditors have access to many tools, programs and software that save
time, produces more relevant findings and point out the critical areas that should be further
investigated by forensic accountants. Some of the common tools used in the audit of financial
statements are: Benford’s Law, Beneish Model, ratio analysis, data mining. This study will focus
on the Beneish Model financial statement fraud detection tool as one cost-effective and efficient
tool that should be utilized by auditors.
Beneish (Beneish M-Score) Model extracts the necessary data from the balance sheet, income
statement and statement of cash flow, and uses eight variables (days sales in receivables, gross
margin index, asset quality index, sales growth index, depreciation index, sales, general and
administrative expenses index, total accruals to total assets index, and leverage index) as
indicators of companies prone to manipulate financial statements. Companies with the higher
Beneish score are more probable to manipulate financial statements.
Fraud Triangle
The first step in fraud investigation is to understand the motives behind the fraud commitment.
The forensic accountant has to investigate why fraudster commits the fraud, under which
circumstances and what is used as fraud justification or rationalization. These three elements are
perfectly depicted in so-called fraud triangle which was developed by Donald Cressey in 1973,
what is shown in the figure below.
Figure 1: Fraud Triangle

Source: Singleton T.W. &amp; Signleton A.J. (2010). Fraud Auditing and Forensic Accounting. Wiley.

253

�The fraud triangle was created after Donald Cressay had interviewed 200 people accused for
embezzlement. Cressay had discovered that each fraud had three elements in common: pressure
(motivation or need), knowledge or opportunity and rationalization (Singleton and Singleton,
2010).
Pressure, incentive or motivation is related to the something that is happening or has happened in
the fraudster’s personal life what forces him or her to commit the fraud, for example: financial
difficulties, bad habits such as gambling, or incentive such as bonus payments based on the
performance (Singleton and Singleton, 2010).
Opportunity is related to the knowledge and experience with respect to the fraudster working
environment. Fraudster will utilize the weaknesses of internal control, familiarity with the
environment and trust given to him to commit the fraud (Singleton and Singleton, 2010).
Rationalization is related to the way in which fraudster justifies his or her fraudulent actions. It is
interesting that, according to the ACFE Report to the Nation from 2008, 93% of fraudsters did
not have criminal record, and it is not rare situation that fraudsters are religious people (Singleton
and Singleton, 2010).
Types of financial statement fraud
According to the ACFE Report to the Nations on Occupational Fraud and Abuse (2016) the
financial statement fraud occurs in 9.6% of cases, with the median loss of $ 975,000. The
intention of fraudster is to misstate the financial statement entries or disclosures to trick the users
of financial statements.
The financial statement fraud is classified into two groups: financial and non-financial. Within
financial group there is further classification on asset/revenue overstatement and asset/revenue
understatement. Since the focus of this research paper is on the financial aspect of the financial
statement fraud the following common types of fraud will be further explored: timing
differences, fictitious revenues, concealed liabilities and expenses, improper disclosures and
improper asset valuations.
Association of Certified Fraud Examiners (2017) describes the types of the financial statement
fraud in the following way:
Timing differences refers to the incorrect treatment of sales where the revenues and expenses are
shifted from one period to the another, affecting the earnings in a desired way. For example, the
inventory is recorded as a sale, knowing very well that part of it will be returned back or twoyear service contract is treated as the revenue of the current year what leaves the consequences
on the future period earnings.

254

�Fictitious revenues are related to the sales that are never realized, which not rarely include fake
customers, what leaves the impact on the revenues, profits and assets. The common sign of
fictitious revenues are obscure accounts receivable that are overdue for a long period of time.
The companies in a financial problems are prone to record fictitious revenues.
Concealed liabilities and expenses refer to the incorrect treatment of liabilities what usually
happens at the end of the accounting period where liabilities are moved to the first month of the
consequent period or when the company is large enough, liabilities are moved to the subsidiary
companies, which are either not being audited or they are audited, but by a different audit
company.
Improper disclosures refer to the obligation of the management to disclose all relevant
information in the financial statements. Improper disclosures related to the financial statement
fraud usually include the following: omission of liabilities, subsequent event, related-party
transactions and management fraud.
Improper asset valuation refers to the incorrect statement of asset amounts (accounts receivable,
inventory, business combinations, long-lived or fixed assets), capitalization of expenses, or
deflating the contra-asset amounts (allowance for doubtful accounts, accumulated depreciation).
Through improper statement of the assets, contra-assets and expenses the financial indicators
will show a better than a true equity and profit values.
Figure 2: Part of Fraud Tree

Source: ACFE (2017)

255

�Schilit’s seven shenanigans
Schilit and Perler (2010) in their book Financial Shenanigans, have identified seven financial
“sins” related to the earnings manipulation, which are:
 recording revenue too soon
 recording bogus or fictitious revenues
 boosting income with one-time gains
 shifting current expenses to later period
 failing to disclose all liabilities
 shifting current income to later period
 shifting future expenses to current period.
According to the research done by Isakovic-Kaplan and Delalic (2013), the comparison is made
regarding the frequency of seven financial shenanigans in the world and in the Bosnia and
Herzegovina, what is summarized in the table below:
Table 1: Comparison of seven financial shenanigans between the world and Bosnia and Hezegovina
WORLD

BOSNIA AND HERZEGOVINA

Recording revenues too soon

Recording revenues too soon

Recording bogus revenue

Recording bogus revenues

Boosting income with one-time gains

Shifting future expenses into the current period

Shifting current expenses to a later or earlier period

Shifting current expenses to a later or earlier period

Failing to disclose all liabilities

Shifting current income to a later period

Shifting current income to a later period

Recording bogus expenses

Shifting future expenses into the current period

Failing to disclose actual revenues

Source: Adjusted from Isakovic-Kaplan and Delalic (2013). Creative accounting in companies in B&amp;H

By looking at the table above, the first two fraudulent practices are common to the B&amp;H and the
world. However, the four out of five remaining financial shenanigans show that companies in
B&amp;H are prone to demonstrate lower net income through increase in expenses or by moving
future expenses to the current period. The possible reason behind this kind of situation is the fact
that BH companies usually do not have established reward system based on the performance, so
there is no incentive to increase revenues. So the top management of BH companies is usually
motivated to commit the fraud against the government through showing lower net income what
implies lower income taxes to be paid to the government.

256

�Red Flags of Financial Statement Fraud
Almost every fraud has warning signals detected in its financial statements, which are commonly
called red flags. According to the ACFE (2017) the red flags that are usually detected in financial
statements are anomalies in profitability, cash flow, assets, liabilities, equity accounts, anomalies
in relationships between financial statement items. Warshavsky (2012) argues that accruals are
very often used as the basic component in the earnings manipulation. The purpose and size of
accruals should serve as one of the important instruments that should assist the forensic
accountant in detection of financial statement fraud, or earnings manipulation.
Methodology
Beneish M-Score Model
Beneish M-score is the mathematical model developed by Messod Beneish which uses eight
variables derived from the company’s financial statements (balance sheet, income statement and
statement of cash flow) with the aim of detecting the companies prone to manipulate its financial
reports (Beneish, 1999).
The variables that are included in the Beneish model are financial ratios computed from the
financial statements for two consecutive years. The formulas for variable computations are
shown in the table below:
DSRI – days sales in receivables index – increase in receivables that is not proportionate to the
sales may be sign of revenue inflation (Beneish, 1999).
GMI – gross margin index – if the GMI index is greater than 1, it means that gross margin have
declined and Lev and Thiagarajan (1993) argue that it is negative sign regarding company's
performance. So there should be a positive relation between increase in GMI and probability of
manipulated earnings (Beneish, 1999).
AQI – asset quality index – if AQI is greater than 1, it is the indication of company's potential
involvement in cost defferal. So, as in the case of GMI index, there is positive relation between
increase in AQI and manipulated earnings (Beneish, 1999).
SGI – sales growth index – growth does not necessiraly indicate manipulation, but when large
companies are exposed to pressure, there is greated probability that their earnings will be
manipulated. The positive relation is expected between SGI and manipulated earnings (Beneish,
1999).

257

�DEPI – depreciation index – if DEPI index is greater than 1, it means that the rate at which assets
depreciate have slowed down, indicating that the company has examined its estimates of assets
useful life. The positive relation between DEPI index and earnings manipulation is expected
(Beneish, 1999).
SGAI – sales general and administrative expenses index – the increase in SGAI is positively
related to the manipulation of earnings (Beneish, 1999).
LVGI – leverage index – if LVGI is greater than 1, than it represents an indication of increase in
leverage. This variable is included in the model with the aim of analyzing debt agreements
incentives for manipulatation of earnings (Beneish, 1999).
TATA – total accruals to total assets – the variable is used in the model with the aim of
analyzing the extent to which cash corresponds to the reported earnings. It is expected that
greater positive accruals are related to the increased likelihood of earnings manipulation
(Beneish, 1999).
Study hypothesis
H1 – The companies in Bosnia and Herzegovina are prone to manipulate financial statements.

258

�Table 2: Variables used in Beneish Model

Source: Anh and Lihn (2016)

259

�The study examines 31 randomly selected company from the Tron Systems database for the
period 2013-2014. The company’s financial statement data is analyzed using Beneish M-Score
model.
5 variables model:
M = -6.065+ .823 DSRI + .906 GMI + .593 AQI + .717 SGI + .107 DEPI
8 variables model:
M = -4.84 + .920 DSRI + .528 GMI + .404 AQI + .892 SGI + .115 DEPI -.172 SGAI + 4.679
Accrual to TA - .327 Leverage
If the M-score is greater than -2.22, there is an indication that company is a potential manipulator
of financial statements data. Otherwise, the M-score lower than -2.22 indicates that the company
is not prone to manipulate its financial statements.
Out of 31 companies, 21 had the M-score lower then -2.22, indicating that there is no potential
manipulation of financial statements data. Six companies had the M-score greater then -2.22,
what points out that they have manipulated their financial statements. Four companies were
classified as non-manipulators according to the 5-variable model, however, after adding three
more variables to the model (accruals to total assets, sales general and administrative expenses,
and leverage index), they were classified as manipulators.
Table 3: Descriptive statistics of sample (n=31 company)
Variable

Mean

Median

Stan.dev.

Min

Max

DSRI

0,98

0,95

0,57

0,16

3,05

GMI

0,87

0,99

1,11

-4,31

3,00

AQI

0,93

0,80

1,55

-0,14

8,61

SGI

1,47

1,03

1,71

0,32

7,69

DEPI

1,04

0,97

0,28

0,59

2,12

SGAI

1,20

0,90

1,28

0,17

7,50

TATA

0,04

0,02

0,10

-0,05

0,57

LVGI
5-variable
model
8-variable
model

1,28

1,05

0,89

0,60

4,32

-2,75

-3,11

1,81

-7,02

2,08

-2,10

-2,37

1,50

-4,11

1,96

260

�The analysis showed that the common variables that were manipulated are gross margin, days
sales in receivable, sales growth, and asset quality. When gross margin decreases from year to
year, there is greater possibility that the company will manipulate the sales and cost of goods
sold. The increase in day sales in receivable signals that the company’s policy regarding
accounts receivable has weakened, so when DSRI is greater than 1, there is greater likelihood
that the receivables will be manipulated, leading to the conclusion that revenues will be inflated.
Also, the increase in asset quality index could be related to the cost deferral or capitalization of
expenses.
The findings indicate, that consistent with the Schilit’s seven shenanigans, Bosnian companies
prone to commit financial statement fraud, are manipulating usually with the sales revenue and
expense capitalization in order to improve the financial statements performance.
Furthermore, the R square for the GMI, AQI and SGI was 45.8%, 21.0% and 26.8%,
respectively, with the level of significance lower than 0.05, what leads to the conclusion that
there is a significant relationship between GMI, AQI, SGI and financial statement fraud.
Conclusion
The purpose of the study was to analyze the current situation in Bosnia and Herzegovina related
to the degree to which companies are exposed to the financial statement fraud. For that purpose,
the financial statements data was obtained from the Tron Systems for 31 company. The obtained
data was analyzed using Beneish M-score model, which is eight variables model that was
developed by professor Messod Beneish in 1999.
Findings revealed that 16% of the analyzed companies are prone to manipulate their financial
statements data, where sales revenues and capitalization of expenses were two main areas where
manipulations were done. The regression analysis showed that gross margin index, asset quality
index and sales growth index significantly influence the Beneish M-score.
This research is a first step in the more detailed investigation of financial statement fraud among
BH companies, since the audit and forensic accounting profession is not yet fully developed in
the country. Also, the Beneish M-score model could be utilized by the auditors in BiH as a time
and cost efficient tool in the financial statement audit. In that way the attention would be drawn
to the areas, accounts or items that should be further explored by forensic accountants, providing
in that way space for development of forensic accounting profession.

261

�References
Anh, N.H., &amp; Lihn, N.H. (2016). Using the M-score model in detecting earnings management:
evidence from non-financial Vietnamese listed companies. Journal of Science: Economics
and Business, Vol. 32.
Association of Certified Fraud Examiners (2016). Report to the Nations on Occupational Fraud
and Abuse.
Association of Certified Fraud Examiners (2008). Report to the Nations on Occupational Fraud
and Abuse.
Association of Certified Fraud Examiners (ACFE) (May 2017). Financial Transactions and
Fraud Schemes. Retrieved from ACFE website:
http://www.acfe.com/uploadedFiles/ACFE_Website/Content/review/examreview/12accoutning-concepts.pdf
Beneish, M. (1999). The detection of earnings manipulation. Financial Analyst Journal.
Isaković-Kaplan, Š., &amp; Delalić, A. (2014). Kreativno računovodstvo u privrednim društvima u
BiH, Međunarodna naučno stručna konferencija: Borba protiv prevara i korupcije 2014,
Forenzika i prevencija Beograd.
Schilit, M.H., &amp; Perler, J. (2010). Financial Shenanigans: How to detect accounting gimmicks &amp;
fraud in financial reports. McGraw Hill.
Singleton, T.W. &amp; Signleton, A.J. (2010). Fraud Auditing and Forensic Accounting. Wiley.
Warshavsky, M. (2012). Analyzing earnings quality as a financial forensic tool. Financial
Valuation and Litigation Expert, issue 39.

262

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                <text>Abstract: One of the greatest challenges faced by auditors is to detect anomalies in financial statement   reporting. Once the anomalies are detected they have to be further investigated by forensic accountants.  However, the practice of forensic accounting has not yet become a reality in Bosnia and Herzegovina. So  the main purpose of the study is to analyze to which degree BH companies are exposed to the financial  statement fraud and with respect to that the need for forensic accountants. The financial statement data  will be collected from BH companies and it will be analyzed using Beneish model. The Beneish model is  the mathematical model that identifies the manipulation of earnings through financial ratios. These  analytical techniques should reveal the unconventional variations in financial statement reporting,  indicating that there is possibility of fraudulent transactions.    Key words: Beneish model, fraudulent reporting, forensic accounting</text>
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                    <text>Rural Business Development in the Balkan Region: Hospitality and Tourism
Management
Gejsi Bendo
Epoka University – Faculty of Economics and Administrative Sciences
Banking and Finance
Albania
gejsibendo98@gmail.com
Abstract: Balkan is part of Europe, but in the past years it has not been known like that and negative
effect of it continue also now days with not being part of European Union and not having profit from
that. Balkan states have been under stratocracy and this has caused to them to not have the knowledge
about management and last methods how to make profit from rural regions and from this we have the
effect of immigration. The economy of Balkan has been underestimated from the other parts. Balkan
region, especially rural areas do not have the infrastructure to make them frequented from the tourists.
Infrastructure is one of the main problems which is related with the electricity, 24h water, Wi-Fi areas
etc and this causes problems to the natives, services and goods that they offer and with the domestic
production.
Tourism should give one of the main revenues in the economies of these states like Albania, European
part of Turkey, Serbia, Macedonia etc, together with the agriculture too. Population of the Balkan is
known for its hospitality and people speak different languages like English, Italian, Spanish and now
days they can speak Turkish too. This characteristic give to them an opportunity that other problems of
technology and infrastructure cause to them disadvantages and from this the tourism is not developed as
much as it had to be. There are a lot of places which are virgin and not explored from foreigners (for
example in Albania or Kosovo and Macedonia too, as well as in other countries which are part of
Balkan). The governments of these countries and their policies do not offer opportunities enough to be
promoted. Since they have been isolated from other parts of the World, most of the people do not know
how to manage with the three levels of the managing (in rural areas) and the only type that exists is just
the sole-managing. There are not enough advertisements or not good marketing in the Balkan region to
make them known. Still there are countries which do not know where the Balkan is.
In continue of this research will be attached what can governments do to solve this problem and how this
problem can be solved about the rural areas which are more than underestimated even if they keep
precious values.
Keywords: underestimated, values, profit, infrastructure, three-level management, domestic production.

Intorduction
Balkan is located in the southeastern of Europe and it is made up from Bosnia and Herzegovina,
Serbia, Albania, Europe part of Turkey, Kosovo,Greece, Montenegro, Croatia, Bulgaria,
Romania and Macedonia. All of the 11 countries are in developing stage so their economy is
based mostly in the agriculture rather than in services.
70

�The problem of these countries is that their capital cities are the most visited ones, while the
rural zones are completely on backstage. This problem is not part of the countries like
Switzerland, which has a lot of touristic places to be visited for or Germany or other countries
which are developed. There are different factors why this part of Europe is in developing stage
starting from history which is related with political factors and wars and so on. All of these
factors has brought these problems to the Balkan, which still is in that position taking loans from
BE and here is the example of Greece which every year takes loans from BE.
There are different types of tourism and here we can include: Culinary tourism, sport tourism,
educational tourism, history tourism, and nature tourism, discover tourism. All of these types
have their differences and each of these is part of every country. Balkan countries especially
rural zones of Balkan do not have developed any of these types, because they are not still
discovered from the tourists who visit Balkan. Last 5 years the North part of Albania is being
heard from Albanians and i take Theth or Valbona as an example.
Except the types of tourism which are listed before we have two other definitions which are:
Rural tourism and Tourism Management.
Rural tourism
It focuses on actively participating in a rural lifestyle. It can be a variant of ecotourism. Many
rural villages can facilitate tourism because many villagers are hospitable and eager to welcome
(and sometime even host) visitors. Agriculture is becoming highly mechanized and therefore,
requires less manual labor.
Tourism Management
It is the leading international journal for all those concerned with the planning and
management of travel and tourism.
As it is said up to the definition of the rural tourism, Balkans are known for their hospitality.
Hospitality is one characteristic of Balkan that everyone appreciates for. It is main characteristic
that tourist are attracted for, despite the nature. Balkans are polyglots so they are able to speak
different languages like English, Italian, Turkish, some Spanish and German and this is an
advantage for the natives who offer different services for example in restaurants, hotels, hostels
and so on.
According to the World Economic Forum, The Travel and Tourism Competitiveness Report
2017, Balkan countries had an average of 3.8/7 (without Kosovo which do not have any statistic
in the WEF)1. Countries are ranked according to the Business Environment:
Safety and Security, Health and Hygiene, Human Resources and Labor Market, Prioritization of
Travel &amp; Tourism, Ground and Port Infrastructure, Price Competitiveness, Tourist Service
71

�Infrastructure, Air Transport Infrastructure, Natural Resources ,Cultural Resources and Business
Travel.
The disadvantages of rural zones are:
Infrastructure:
Rural zones of Balkan have a lot of problems related with infrastructure. Roads have a bad
quality and can not be safe to drive through, there is a missing of electricity most of the time and
water too. Another problem related with the infrastructure is Wifi zones or at least to have some
internet or just to be able to call or text.
Government’s policy and expenditures:
Government’s policy and expenditures are related with what governments are doing to rise up
profits from the tourism. This is not related just with increasing taxation, but with what they
offer to the natives for the development of the tourism in these zones.
Investments:
Since there is no government’s policy or expenditure, foreign investors or even natives one are
going away from these rural zones. Even if last years it has changed still investments are not in
that level where they had to be. In these rural zones for example in Albania there exist just few
guest houses which offer the role of the hotels.
Management:
In rural zones the only type of management is the sole management because each of them wants
to be independent from the others, even if they offer same service and no one of them changes
something to be different and attract more. They think just to increase their incomes and not to
work together to do something different.
Advertisement:
Advertisements are related with management, since each of them is in a sole managing, so the
advertisements which are one of the main keys to the business are not well developed.
Economy of Balkan still is in developing phase and it needs time to become in the same level as
the other countries. But tourism is one part to be more developed in Balkan zones, but not just
main/capital cities, but also rural zones which can increase profits and GDP more than the other
parts which now have been visited from the tourists and natives too.
According to some researches that what can be done which do not have too much cost for the
rural zones are listed above:
• Improve AIRLINE /RAILWAY /HIGHWAY- which are main factors of these zones to
be more developed.
•

Water and electricity 24h- related with the infrastructure.
72

�•

Different courses for managing, since the only way is the sole proprietorship and this
means sole managing.

•

Employee managers to each of the hotels or restaurants paid from government to help
increasing management knowledge.

•

Courses for learning English or other languages in rural zones.

•
•

Credits without interest % to help them improve their managing and services
Gather them together to share ideas and take decisions and plan which are their strategic
points and what can they offer so they can be unique on their type

•

Free advertising-

•

Advertising is needed to make them known; these places should be in Google Map and
applications like this so everyone can be able to check them out.
A 12 month touring for each: Different cities or countries have different seasons which
have more tourists, but this should be changed from the governments are natives. Each
city should be visited not only in summer because of the nature, but also in winter
because of the culinary tourism etc.

•

Help to innovate their houses from investors:

•

Policies from governments should attract the investors in these zones, but also from
advertisements they should be affected.
Building and biding trust with them (B&amp;B)

As the Bed and Breakfast webpage to book a room or a bed in a place to stay, where bed and
breakfast are related so strong together with each other, we should build and bind trust with each
other and we should believe that Rural zones of Balkan will be developed.
Balkan doesn’t mean to come and visit once, it means to come and visit and explore in every
detail. Capital cities are not the treasure; rural zones are the real treasure which keeps high
values from cultural, historical etc.
Let’s make the Balkan Rural Zones be the most frequented and complete their needs, so GDP
will increase and countries will pass in another stage, the stage of developed countries!

References:
http://reports.weforum.org/travel-and-tourism-competitiveness-report-2017/

73

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                <text>Bozalija, Berin
DUMAN, Teoman</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="94">
            <name>Abstract</name>
            <description>A summary of the resource.</description>
            <elementTextContainer>
              <elementText elementTextId="1034">
                <text>Abstract: Aware of the effect on the domestic production is a huge process which includes numbers of different research on various theory which are explaining the terms aware of somethinh in a term of buying somethinh or product. Many researchers help us to understand this problem and to open some other research about this and also to understand the human behavior and their decision, also it is very complex concept and it consider what is difficult to understand human preferences in buying product from outside or it means foreign product or to buy domestic product that is made in you own country,there are relationships on the market and also some other factors which are taking a part in this interaction.Customer loyalty in general is the behavior which customer play main role in my terms named by Bosnian people and there preferences to domestic or foreign brands and also to understand their preferences in the decision making process. In a term of consideration set influences on consumer decision making and choice,issues,suggestion or models. Bosnian people aware about buying the domestic product is the purpose of this study is to define and analysis the relationships between consumers and domestic products. The purpose of this research is to degine consumers and their responsibility to buy domestic product. Emipirical finding reveal that why consumer prefer to buy domestic or foreign products tend to have uniques lifestyle and great shopping orientation that is different from those who prefer domestic. Our study brings previous research about preferences in buying domestic products and foreign products and all aspects that which are connected with his theory and model concept. Result of this study help an organization to recognize what components is missing to satisfied customer satisfaction. After this study it will be useful for an organization or company exactly to decide what to do, when, why, where and how to do or they will find anothery way to satisfied customer needs.  Key words : Customer loyalty, purchase decision, brand awareness, post-purchase behavior and social responsibility.</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="40">
            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="1035">
                <text>2017</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="97">
            <name>Keywords</name>
            <description>Keywords.</description>
            <elementTextContainer>
              <elementText elementTextId="1036">
                <text>Conference or Workshop Item
PeerReviewed</text>
              </elementText>
            </elementTextContainer>
          </element>
        </elementContainer>
      </elementSet>
    </elementSetContainer>
    <tagContainer>
      <tag tagId="6">
        <name>H Social Sciences (General)</name>
      </tag>
    </tagContainer>
  </item>
</itemContainer>
