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                    <text>3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Effect of steel plant pollution on photosynthetic apparatus of some spontaneous plants
by chlorophyll fluorescence imaging
EriolaZhuri Hida1, VeledinÇako2,FatbardhaBabani3,Theodhor Karaja4
1University ‘AleksanderMoisiu’, Durres, Albania,
2 Department of Physics, University ‘Ismail Qemali’,Vlora, Albania,
3 Biotechnology Department, University of Tirana, Tirana, Albania,
4Physics Department, University of Tirana, Tirana, Albania,
E-mails: eri_zhuri@yahoo.com, v.cako69@hotmail.com, fbabani@yahoo.com,
theodhor.karaja@yahoo.com
Abstract
Environmental pollution by the steel plant in Elbasan, the largest metallurgical complex in
Albania causes serious problems. Although the blast furnaces and basic oxygen converters
closed in 1991, small scale steel production from scrap metal continued. This steel plant
releasing chemicals and particulates into the atmosphere is considered a source of air
pollution.
Industrial siderurgic air pollution as other stressors affects growth and physiological functions
of plants. Pollution has effects on the photosynthetic performance of leaves and can modify
their optical and fluorescence properties either directly or indirectly. The chlorophyll (Chl)
fluorescence provides ample information on the performance of photosynthetic apparatus. Chl
fluorescence signatures of leaves as an efficient tool and a nondestructive method for the in
vivo analysis of plant stress is applying to describe and investigate the photosynthetic light
processes and quantum conversion at physiological conditions as well as to detect stress on
the photosynthetic apparatus. The chlorophyll fluorescence images were measured on leaves
using the FluorCam 700MF imaging system (Photon Systems Instrument) as a techniques that
offer the possibility to study the distribution and patchiness of fluorescence signatures over
the whole leaf area.
Chl fluorescence images were measured on the leaves of some spontaneous plants grown in
sites with different level of steel plant air pollution assessed on base of different distances and
different directions from the source of the pollution. Efficiency of photosynthetic apparatus of
analyzed plants was evaluated via chlorophyll fluorescence images during induction kinetics
and various fluorescence ratio images. The images of Chl fluorescence ratios, acquired by
pixel to pixel arithmetic operations performed by FluorCam software, were aplied as
indicators of the functional state or the damage of the photosynthetic apparatus. The potential
and effective quantum yields of photosynthetic electron transport were estimated too.
Observed differences on imaging of chlorophyll fluorescence signature and photosynthetic
pigment content of some spontaneous plants allowed characterizing of the photosynthetic
performance in order to evaluate the damage by plant steel air pollution.

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Keywords: chlorophyll fluorescence imaging, induction kinetics, plant steel air pollution,
photosynthetic apparatus, potential/effective quantum yield, spontaneous plant.
1.INTRODUCTION
Environmental pollution by the steel plant in Elbasan, the largest siderurgical complex in
Albania causes serious problems. In this area operate some plants as steel plant operator
"Kurum“, cement factory "ECF", Establishment of Ferro-Chrome "ACR“.
This steel plant releasing chemicals and particulates into the atmosphere is considered a
source of air pollution. Monitored elements in the Elbasan area are:
- solids suspended in the air (LNP), rigid particles with dimensions smaller than 10 microns
(PM10)
- lead (Pb), sulfur dioxide (SO2), nitrogen dioxide (NO2), ozone (O3) in the air.
Siderurgic air pollution as other stressors affects growth and physiological functions of plants.
Pollution has effects on the photosynthetic performance of leaves and can modify their optical
and fluorescence properties either directly or indirectly. The chlorophyll (Chl) fluorescence
signatures of plants have been applied as an efficient tool to describe and investigate the
photosynthetic light processes and quantum conversion to detect pollution effects on the
photosynthetic apparatus. Image analysis of the Chl fluorescence signals offers the possibility
to study the function of photosynthetic apparatus and to detect early stress damage in plants
by screening the fluorescence emission over the leaf area as well as the gradients and local
irregularities in fluorescence emission and ratios (Lichtenthaler 1996, Buschmann and
Lichtenthaler, Krause and Weis, 1991; Lichtenthaler and Miehé, 1997; Lichtentaler and
Babani, 2004; Schreiber1986). Various parameters and ratios of the Chl fluorescence
determined from the induction kinetics (Kautsky effect) can be used as indicators of the
functional state or stress damage of the photosynthetic apparatus and photosynthetic electron
transport (Babani and Lichtenthaler, 1996; Buschmann and Lichtenthaler, 1998; Govindjee
1995, 2004). Imaging of Chl fluorescence kinetics correctly screening the emission
heterogeneity reflects localized biotic or abiotic stress or heterogeneous metabolism. Offering
the possibility to study distribution and patchiness of fluorescence signatures over the whole
leaf area these techniques were developed as invaluable tool for determining the
photosynthetic performance of plants (Buschmann and Lichtenthaler 1998, Lichtenthaler and
Babani 2000; Lichtenthaler et al., 2000,Lichtenthaler and Babani 2004, Lichtenthaleret al.,
2007, Nebdal et al. 2000, Schreiber 2004).
The objective of the presented research is the evaluation of damage by plant steel air pollution
on spontaneous plants characterizing the photosynthetic performance by imaging of
chlorophyll fluorescence signature. Imaging of Chl fluorescence kinetics correctly screening
the emission heterogeneity reflects localized biotic or abiotic stress or heterogeneous
metabolism. Offering the possibility to study distribution and patchiness of fluorescence
signatures over the whole leaf area these techniques were developed as invaluable tool for
determining the photosynthetic performance of plants

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2.MATERIALS AND METHODS
2.1. Plant material
Endemic-spontaneous plant Cercius siliquastrum grown in different steel plant pollution
conditions were analyzed. Study areas were chosen in three different locations: Dajti area (site
1) characterized by optimal physiological conditions, Krrabe area (site 2 2.6 km) and Elbasan
area(site3- 6 km)characterized by plant steel air pollution.
Chlorophyll fluorescence imaging of induction kinetics
Chlorophyll (Chl) fluorescence induction kinetics were measured using the FluorCam 700MF
kinetics imaging system constructed by Photon Systems Instrumentto capture kinetics and 2dimensional maps of key fluorescence parameters. The fluorescence emission is induced by
two sets of 325 super-bright orange light emitting diodes (LED’s) (wavelength 605nm) that
provide excitation flashes or a continuous actinic irradiance controlled by defined protocol.
Fluorescence images are captured by CCD camera. The images are taken at 12-bit resolution
in 512 x 512 pixels of CCD chip. The size of an analyzed object is smaller than 10  13 cm.
The chlorophyll (Chl) fluorescence images and induction kinetics were measured on predarkened leaves (30 min) using the FluorCam quenching protocol. The images of the
measured Chl fluorescence intensity were obtained on false colour, whereby black is the
lowest (zero) and red the highest fluorescence intensity.
Chlorophyll fluorescence images of parameters as F0 and F0’(minimum fluorescence in the
dark and in the light-adapted states), Fm and Fm’ (maximum fluorescence in the dark and in
the light-adapted states), Fp, (initial fluorescence increase caused by the actinic light
exposure) and Fs, (steady-state fluorescence in actinic light exposure) were recorded. Images
of various Chl fluorescence ratios obtained by pixel to pixel arithmetic operations performed
by FluorCam software were: maximum quantum yields of Photosystem II Fv/Fm and Fm/Fo;
effective quantum yields of Photosystem II Fv'/Fm‘andFm’/Fo’; fluorescence decline ratio in
steady-state which assess plant vitality Rfd=(Fp-Fs)/Fs; non photochemical quenching
coefficients NPQ=(Fm-Fm’)/Fm and qN=(Fv-Fv’)/Fv; where Fv=Fm-Fo and Fv’=Fm’-Fo’.
Kinetics of the fluorescence transient over the leaf area was performed by FluorCam software
where each data point represents one image. The represented induction kinetics are the mean
curves of six different kinetics from six different leaves grown in every study area.
3.RESULTS AND DISCUSSION
3.1.Image Fluorescence parameters
Fully green leaves of Cercius siliquastrum grown in optimal physiological conditions (Dajti
area-Site 1) from six different branches were analyzed. Images of the maximum fluorescence
in the dark Fm of all analyzed leaves exhibited almost the same distribution as well as the
same level of fluorescence signal over the whole leaf area. The images represented in Figure 1
(A) that belong to one of these leaves showed no irregularities and nearly uniform distribution
of fluorescence signatures.

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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

A

B

C

Figure 1. Images at the maximum fluorescence in the dark Fm of a leaf grown in optimal
physiological conditions A-site 1, and plan steel air pollution conditions B-site 2, C- site 3
(pseudoscale 0-700).
Images at the maximum fluorescence in the dark Fm of leaves grown in plan steel air
pollution conditions showed a different distribution of the fluorescence emission over the leaf
area as compare to the leaves grown in optimal conditions, especially leaves grown in site 3
(Figure 1, B, C). Fluorescence images at Fm displayed at the same pseudoscale clearly
showed changes of the values of this parameter and their distributions related to the
heterogeneity over leaf area between leaves grown in different conditions.
The shape of the fluorescence induction kinetics of green leaves grown in optimal conditions
demonstrated the healthy physiologically state of these leaves (Figure 2, A). While the shape
of the induction kinetics exhibiting an increase of the fluorescence signal at the steady state of
fluorescence demonstrated the effect of pollution to which were exposed leaves during their
growth (Figure 2, B, C).

A

B

Figure 2.Induced fluorescence kinetics of leaves of Cercius siliquastrumgrown in optimal
physiological conditions A-site 1, and plan steel air pollution conditions B-site 2, C- site 3.
Image fluorescence parameters of every leaf were calculated by FluoCam software as the
mean of the fluorescence signals of all pixels over the leaf area. The represent values of these
parameters (Table 1) correspond to a mean of six different leaves. The mean values of image
fluorescence parameters demonstrated the differences between leaves grown in different
conditions. The values of standard deviations of fluorescence parameters of leaves grown in
optimal conditions show no significant differences between analyzed leaves. The observed
increase of the values of standard deviations from optimal growth conditions to pollution
conditions can be illustrated the increase of the variability through the leaves as the effect to
stress exposure.
90

C

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Table 1. Induced fluorescence image parameters of leaves of Cercius siliquastrum grown in
area of optimal and plan steel air pollution conditions (mean of six leaves).

Cerciussiliquastrum
Image Fluorescence
Fo
parameters

Fm

Fv

Fo'

Fm'

Fv'

mean

130.8

406.8

276.3

141.7

181.4

40.0

std

(3.36)

(2.62)

(5.39)

(3.92)

(4.87)

(1.88)

mean

140.5

484.4

343.9

161.3

209.5

48.3

std

(4.35)

(39.60
)

(39.62
)

(16.5)

(18.29
)

(5.54)

mean

118.4

333.2

214.8

149.9

193.4

43.4

std

(5.54)

(64.12
)

(61.32
)

(26.76
)

(23.52
)

(13.49
)

Site 1

Site 2

Site 3

3.2.Image Fluorescence ratios
Images of the fluorescence decline ratio Rfd of a green leaf grown in optimal conditions
showed almost no irregularities and a uniform distribution of the values of this ratios over the
leaf area (Figure 3 A). The values of Rfd ratios as plant vitality indicator demonstrated that
these full green leaves can be characterized by the high photosynthetic activity, as reflect by
the mean values the ratio 1.63 (Table 2).

A

B

C

Figure 3. The fluorescence decline ratio image Rfdof leaves of Cerciussiliquastrum grown in
optimal physiological conditions A-site 1, and plan steel air pollution conditions B-site 2, Csite 3 (pseudoscale 0-3).
The images of the fluorescence decline ratio Rfd of leaves grown in pollution conditions
(Figure 3 B, C) showed a non uniform distribution and increase of irregularities of the values
of this ratios over the leaf area.
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Table 2. Image fluorescence ratios of leaves of Cercius siliquastrum grown in area of optimal
and plan steel air pollution conditions (mean of six leaves).

Cerciussiliquastrum
Image
ratios

Fluorescence

Fm/Fo

Fv/Fm

Fm'/Fo'

qN

NPQ

Rfd

mean

3.15

0.684

1.27

0.856

1.240

1.63

std

(0.09)

(0.009)

(0.02)

(0.005)

(.053)

(0.10)

mean

3.09

0.66

1.12

0.826

1.732

1.26

std

(0.29)

(0.02)

(0.04)

(0.007)

(0.142)

(0.18)

mean

2.81

0.64

1.29

0.798

0.723

1.14

std

(0.65)

(0.080)

(0.036)

(0.145)

(0.308)

(0.377)

Site 1

Site 2

Site 3

The values of Rfd ratios represented a lower mean values of the ratio comparing to the
optimal conditions, mean values of 1.26. The values of Rfd ratios in pollution growth
conditions represented the lowest mean values of the ratio comparing to two other growth
conditions, mean values of 1.14 (Table 2, Figure 3).
The distribution of the Rfd values over the leaf area as well as the values of the ratio
demonstrated a lower activity of photosynthetic apparatus of the leaves grown in plan steel air
pollution growth conditions in comparison of leaves grown in optimal conditions (Dajti area).
The observed decrease of activity of photosynthetic apparatus in pollution conditions seem to
be related to the distance of the source of pollution, (Figure 3, Table 2).

Figure 4.Fluorescence decline ratio Rfd of leaves in different growth conditions (mean of six
leaves).
The distribution of the Rfd values showed a considerable reduction of the activity of the
photosynthetic apparatus of the leaves grown in area of site 3 than of leaves grown in two
other areas as plants were grown in severe stress-pollution conditions
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

The values of Rfd ratios as plant vitality indicator demonstrated that full green leaves can be
characterized by the high photosynthetic activity, as reflect by the mean The fluorescence
ratios that allow to estimate maximum and effective quantum yields of Photosystem II
(Fv/Fm,Fm/Fo) indicated a decrease of these ratios from optimal growth conditions to stresspollution conditions (Table 2).
Non photochemical quenching coefficient qN have been decreased in growth pollution
conditions (Table 2).
Increase of the standard deviation values were observed in all calculated fluorescence ratios to
pollution growth conditions in compare to optimal conditions. Observe increase can be
illustrated the raise of the variability through the leaves as the effect to pollution exposure.
4.CONCLUSIONS
Fluorescence images measured during induction kinetics, shape of the Chl fluorescence
kinetics and the values of the fluorescence parameters in the leaves of endemic plant
Cerciussiliquastrum grown in optimal conditions (Dajti area - site 1) exhibited a high
photosynthetic activity as is demonstrated by the values of fluorescence ratios which evaluate
the plant vitality and quantum yield of photosynthetic apparatus: Rfd=1.63, Fm/Fo=3.15.
Activity of photosynthetic apparatus of leaves grown in plant steel air pollution conditions
(site 2).was generally lower than activity of plants grown in optimal conditions: Rfd=1.26,
Fm/Fo=3.1.
Activity of photosynthetic apparatus of leaves grown in plant steel air pollution conditions
(site 3) -Elbasan area) demonstrated reduction compare to other areas as is expressed by the
lowest values of fluorescence decline ratio (Rfd=1.14,Fm/Fo=2.81); increased non-uniformity
distribution and heterogeneity of signal of fluorescence over the leaf area and shape of
induction kinetics.
Observed differences on imaging of chlorophyll fluorescence signature and photosynthetic
pigment content of spontaneous plants allowed to characterize the photosynthetic
performance in order to evaluate the damage by plant steel air pollution related to the
distance of the source of pollution.
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                <text>Environmental pollution by the steel plant in Elbasan, the largest metallurgical complex in  Albania causes serious problems. Although the blast furnaces and basic oxygen converters  closed in 1991, small scale steel production from scrap metal continued. This steel plant  releasing chemicals and particulates into the atmosphere is considered a source of air  pollution.  Industrial siderurgic air pollution as other stressors affects growth and physiological functions  of plants. Pollution has effects on the photosynthetic performance of leaves and can modify  their optical and fluorescence properties either directly or indirectly. The chlorophyll (Chl)  fluorescence provides ample information on the performance of photosynthetic apparatus. Chl  fluorescence signatures of leaves as an efficient tool and a nondestructive method for the in  vivo analysis of plant stress is applying to describe and investigate the photosynthetic light  processes and quantum conversion at physiological conditions as well as to detect stress on  the photosynthetic apparatus. The chlorophyll fluorescence images were measured on leaves  using the FluorCam 700MF imaging system (Photon Systems Instrument) as a techniques that  offer the possibility to study the distribution and patchiness of fluorescence signatures over  the whole leaf area.  Chl fluorescence images were measured on the leaves of some spontaneous plants grown in  sites with different level of steel plant air pollution assessed on base of different distances and  different directions from the source of the pollution. Efficiency of photosynthetic apparatus of  analyzed plants was evaluated via chlorophyll fluorescence images during induction kinetics  and various fluorescence ratio images. The images of Chl fluorescence ratios, acquired by  pixel to pixel arithmetic operations performed by FluorCam software, were aplied as  indicators of the functional state or the damage of the photosynthetic apparatus. The potential  and effective quantum yields of photosynthetic electron transport were estimated too.  Observed differences on imaging of chlorophyll fluorescence signature and photosynthetic  pigment content of some spontaneous plants allowed characterizing of the photosynthetic  performance in order to evaluate the damage by plant steel air pollution. Keywords: chlorophyll fluorescence imaging, induction kinetics, plant steel air pollution,  photosynthetic apparatus, potential/effective quantum yield, spontaneous plant.</text>
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Parasitic Diseases And Their Controls In Sustainable Development Of Aquculture Of
Bluefin Tuna (Thunnus Thynnus)
Erol Tokşen, Egemen Nemli,Uğur Değirmenci, Ulviye Karacalar
Ege University, Fisheries Faculty, Department of Fish Diseases, Bornova 5100 İzmir, Turkey
E-mails: erol.toksen@ege.edu.tr, egemen.nemli@ege.edu.tr, ugur.degirmenci@ege.edu.tr,
ulviye.karacalar@ege.edu.tr
Abstract
In the last decades Turkish and Mediterranean mariculture has focused its production mainly
on two species, gilthead sea bream (Sparus aurata L.) and sea bass (Dicentrarchus labrax L.).
41

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

However, due to its high commercial value bluefin tuna (Thunnus thynnus) has been
considered as an alternative aquaculture target. The culture of bluefin tuna has started by
private sector in 2001 in Turkey. The catched fishes are fed by frozen herring, sardina,
mackerel between May and June and after fattened, they are sold. Total feeding time in
Turkey 4 to 10 months like other countries which are culturing bluefin tuna. As an developing
sector in Turkey and in the world, blue fin tuna farming require to further studies on larvae
production, feed investigations and diseases occur on the fish. According to studies conducted
to date, 28 species were found in Pylums Ciliphora, Myxozoa, Platyhelmintes, Aschelmintes
and Arthropoda. The present work aim to reveal diseases of bluefish tuna caused by parasites,
how they are transmitted, which effects they have on tuna fish, how they could be diagnosed,
and how they could be controlled and treated.
Keywords: Bluefin tuna, disease, parasite, diagnosis, treatment.
1.INTRODUCTION
Aquaculture is one of the fastest growing industries in Turkey having grown in
volume by over 20 percent for the past ten years (Table 1). Production of the three major
species, namely rainbow trout (Oncorhynchus mykiss), seabass (Dicentrarchus labrax) and
seabream (Sparus aurata) increased rapidly during the 1990s, with efforts having been given
to the development of new species, such as the Black Sea turbot (Scophthalmus maeoticus)
and some Mediterranean species such as sharpsnout seabream (Diplodus puntazzo), common
seabream (Pagrus pagrus), common dentex (Dentex dentex) and groupers (Epinephelus spp.).
Atlantic bluefin tuna (Thunnus thynnus) fattening, which started at the turn of the millennium
has been the latest development in terms of species diversity.

Species
Seabass/seabream
Trout
Shell
Tuna
Trout &amp; seabass
Seabass &amp; bream &amp; new species
Trout
Seabass &amp; bream &amp; trout
Seabass
Total

Farm number

Rate (%)

Rate (%)

87
1
1
2
4
4

Capacity
(tonnes)
99 869
820
1 925
7 440
9 613
9 529

309
5
4
8
14
13
1
1

0
0

4 800
100

3.6
0.1

1
356

0
100

25
134 121

0
100

74
0.6
1.4
5.5
7.2
7.1

Table 1. Number of marine fish farm, capacities and production in 2009.
(http://www.fao.org/fishery/countrysector/naso_turkey/en)

Tuna aquaculture is presently in its early stages of development and will likely
continue to expand, but with this expansion, parasitic disease will become increasingly
important. Thus, focus has been placed on parasitic infections in these enterprises and their
economic and ecological impact. Diseases problems including parasitic organisms are the
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

main threat to further increas of the industry. There are various parasites caused the diseases
on tuna fish. This research presents the individual parasites types producing problems in tuna
fish. Each section is presented with 1.aetiyology, the parasitic organism responsible for the
disease, 2.epizootiology, the transmission of the diseases and life cycle of the parasite,
3.pathogenicity, how the parasite produces diseases in the fish, 4.symptoms, clinical signs of
the diseases, 5.diagnose, how the infection can be identificated, 6.treatment, how the infection
can be controlled.
2. Coccidiosis
2.1. Aetiology. Goussia auxidis
Mature oocysts (18-28 µm diameter) occur in the splenic pulp and in melanomacrophage
centres in the spleen and liver, and in the peribiliary connective tissues of the liver. The
oocyst has a thin membranous wall, and each contains four sporocysts but no oocyst
residuum. Sporocysts (length 8-12 x 6-7 µm) have a thickened wall with a faint dehiscence
suture occasionally visible (Jones 1990).
2.2. Epizootiology: The method of transmission of infection is unknown. 98 % (140 of 143)
of albacore, T. alalunga, and an individual yellowfin tuna, T. albacares, from the South
Pacific were found be infected (Jones 1990).
2.3. Pathogenicity: Oocysts occurred in both the liver and spleen where they produced
minimal host response. Host response appears to be minimal with slight proliferation of
fibroblasts around the oocyst in some fish. The spores are positively associated with
melanomacrophage centres but the degree of association varies from fish to fish (Jones 1990).
2.4. Symptoms: No clinical signs have been reported.
2.5. Treatment and Prophylaxis: Not required.
3. Uronemosis
3.1. Aetiology: Uronema nigricans
Fixed and stained parasite is variable in size ranging from 19.8-34 µm in length by 7.1-20 µm
in width. They contained distinctive somatic ciliature consisting of 12-14 longitudinal kineties
arranged in meridional rows (Munday et al. 1997).
3.2. Epizootiology: Until recently, infections of fish with Uronema sp. had been mainly
reported in aquarium species in which they were invasive and resulted in severe tissue
damage and subsequent mortalities (Cheung et al. 1980, Bassleer 1983). More recently the
potential for scuticociliatids to be a problem in mariculture has been highlighted by reports of
serious disease of larval and juvenile fish in Japan and Spain attributed to infection with
Uronema sp. (Yoshinaga and Nakazoe 1993, Dykova and Figueras 1994) and of mature sea
bass in the Mediterranean, where parasite was described as Philasterides dicentrarchi
(Dragesco et al. 1995). In Australia mortalities of southern bluefin tuna Thunnus maccoyii in
growout seacages have also been reported with the causative agent being identified as
Uronema nigricans (Munday et al. 1997). Epizootiological factors which may be implicated in
the initiation of the disease in SBT are water temperature and host immune status. The
"swimmer" syndrome has not occurred when water temperatures have exceeded 18°C. SBT
maintain a body temperature of about 24°C, even in much colder water, so it is possible that
43

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

the parasite, which would be expected to have an optimum growth temperature of about 25°C
(Parker 1976), would be preferentially attracted to the fish under conditions of relatively low
water temperatures (&lt; 18°C).
3.3. Pathogenicity: U. nigricans initially parasitizes the olfactory rosette at which stage the
host mounts a vigorous inflammatory response. If the host response is inadequate, the ciliate
then invades branches of the olfactory nerve present in the axis of the olfactory rosette. Even
though there is still some host response to invasion of the olfactory nerve the migration of U.
nigricans is probably then inexorable, ending in invasion of the brain, which causes locomotor
dysfunction and, ultimately, death. Generalised infections leading to severe mortalities in
larval marine fish were reported (Munday 1996).
3.4. Symptoms: Typically, affected tuna fish came to the surface, turned light blue and swam
vigorously around the cage. Eventually, the fish ceased compulsive swimming and exhibited
short bursts of forward motion with their heads out of water, followed by periods of sinking,
before once again coming to the surface and then repeating the process. Finally, the fish sank
and died at the bottom of the netpen (Munday et al. 1997).
3.5. Diagnosis: Presumptive diagnosis of the disease in SBT can be made by examining wet
preparations of CSF and brain. However, although a fluorescent antibody test has been
developed for cultured and environmental organisms (Watts 1995), this is not suitable for
clinical material because of autofluorescence of host tissues. Definitive diagnosis can be made
by microscopic examination of histological slides of nervous tissues. As clinical pathology
only reflects general stress and perturbed osmoregulation, it is of no specific diagnostic value.
3.6. Treatment: The most effective concentrations of formalin were 100 and 200 ppm where
total cell lysis occurred after 120 and 60 min respectively. Hydrogen peroxide was lethal to
the ciliate at all concentrations (Crosbie and Munday, 1999).
4. Kudoasis
4.1. Aetiology: Kudoa prunusi, K. crumena
Typical Kudoa crumena spores measuring 7.5 x 9.9 µm and 4.4-5.6 x 7.8-10.0 µm. Average
spore size of Kudoa prunusi is 9.63 μm in width and 7.50 μm in length (Meng et al., 2011).
4.2. Epizootiyology: K. crumena was reported in a yellowfin tuna (Kent et al. 2001). The
infections described by Langdon (1990) were in southern bluefin tuna caught off
southwestern Western Australia when the fish would have been 1-3 years of age and feeding
on cephalopods, crustaceans and salps (Kailola et al. 1993). Infections have also been
reported in southern bluefin tuna in South Australia (Rough 2000) and wild fish caught off the
New South Wales coast where the prevalence of about 1% affected fish was a cause of
commercial loss. The prevalence of K. crumena in albacore was reported as 5%. Kudoa sp.
infection of northern and southern bluefin tuna producing lesions in the flesh.
4.3. Pathogenicity: The infection in southern bluefin tuna produces white cysts 1-10 mm in
diameter which are apparently in the muscle (Rough 2000) although Langdon (1990)
produced evidence to suggest that most, if not all, cysts in southern bluefin tuna were in
peripheral nerves, especially the intercostal nerves. Histologically the cysts are found to
consist of numerous Kudoa spores surrounded by a fibrous capsule. Similar lesions because of
an unidentified Kudoa sp. have been reported in southern bluefin tuna and Langdon (1990)
suggested that the parasite could be K. nova, but as it does not produce myoliquefaction, this
seems unlikely.
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4.4. Symptoms. None reported.
4.5. Diagnosis. Kudoa sp. can be seen in wet preparations or histological sections. Numerous
plasmodia were localized not only in the cavity of the optic tectum but also in the tissue of
cerebellum. Host inflammatory response and gliosis were occasionally found around
plasmodia (Meng et al. 2011).
4.6. Treatment and Prophylaxis. Neither treatment nor prevention are practicable.
5. Hexacapsulosis
5.1. Aetiology: Hexacapsula neothunni
Spores measure 6.2-11.0 µm and have six shell valves each containing one polar capsule
(Lom and Dykova 1992). Kudoa nova spores measure 5.3-6.5 x 8.5-9.8 µm and have four
shell valves each containing one polar capsule.
5.2. Epizotiology: Most myxosporeans, for which life cycles are known, have a two-host
cycle with the myxosporean in a fish and an actinosporean in an invertebrate (Kent et al.
2001). As most juvenile tuna consume invertebrates such as squid and crustaceans (Kailola et
al. 1993), it is conceivable that these prey species could be alternative hosts.
5.3. Pathogenicity: The postmortem liquefaction of the muscle caused by the release of
proteases from the parasites that is the most dramatic result of the infection (Ogawa 1996).
Histologically, the myxosporean spores are found aggregated in the cystic structures and
usually produce minimal host response.
5.4. Symptoms: The parasites produce no clinical signs, and, while with heavy infections
cysts may be visible in the musculature
5.5. Diagnosis: Typical myxosporean spores can be easily found in wet preparations or
histological sections of affected muscles.
5.6. Treatment and Prophylaxis. Neither treatment nor prevention is practicable.
6. Cardicolosis
6.1. Aetiology: Cardicola ahi, Cardicola forsteri
Adult flukes are dorsoventrally compressed and 100-150 μm in width, with marginal
tegumental spines, parenchymous body cavity, and indistinct reproductive and digestive tracts
(Cribb et al. 2000).
6.2 Epizootiyology: C. ahi has been reported from yellowfin and bigeye tunas (Smith 1997).
C. forsteri (Cribb et al. 2000) occurs in southern bluefin tuna. The intermediate hosts of
Cardicola ahi and C. forsteri are not known. It is also not known if teleosts, other than
Thunnus spp., act as final hosts. Colquitt et al. (2001) reported that the prevalence and
severity of the infection increased with the time that southern bluefin tuna were held in
captivity suggesting that the life cycle was maintained in the vicinity of the cages.
Additionally, tuna farmers have reported that infections tend to be more severe at new cage
sites suggesting that the parasite may also have a deleterious effect on the intermediate host.
6.3. Pathogenicity: Multifocal, white to yellow lesions involving the gills of infected southern
bluefin tuna are described. The lesions ranged in size from 2 to 12 mm and often extended in
an arc across the gills. The lesions appeared to be the result of the fluke ova impacting in the
45

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

afferent filamentary arteries where they stimulated a host response. Cardiac lesions were also
reported and noted many ova surrounded by granulomas. There was marked hypertrophy of
the cardiac spongiosa, presumably because of increased resistance to blood passing through
the partly occluded branchial vasculature (Colquitt et al. 2001). It may not be a coincidence
that bigeye tuna, which are known to be infected with C. ahi, have a much more compact
ventricular myocardium (74%) compared with northern bluefin tuna (30–50%) which have
not been reported to be to be infected with blood flukes (Santer and Greer-Walker 1980,
Smith 1997).
6.4. Symptoms: C. forsteri infections of cultured southern bluefin tuna lead to increased
mucus on the gills and have been associated with signs of respiratory distress, lethargy and
slightly increased mortality (Rough 2000).
6.5. Diagnosis: In southern bluefin tuna the gross lesions are characteristic enough to enable a
presumptive diagnosis. Histopathology is even more diagnostic, but definitive diagnosis
depends upon flushing the adults from the heart and identifying them.
5.6. Treatment and Prophylaxis: Prevention of blood fluke infections depends upon an
understanding of the parasites life cycle and, therefore, is not possible at present.
7. Didymocystiasis
7.1. Aetiology: Didymocystis wedli
The membranous cysts on the gill filament are oval in shape and measure 3-6 mm in length by
1.5-2.8 mm in width, depending on the stage of development and contain two similar worms
applied closely against each other with opposite extremities. Under higher magnification we
can observe the porous surface of the cyst. The characteristic feature of the body shape of
D. wedli, with two distinct parts, can be well observed by light and scanning electron
microscopy. The forebody is elongate, cylindrical, and slender, measures 1-2.2 mm long by
00.1-0.3 mm wide. The tegument of ventral and dorsal surfaces of forebody is wrinkled
cobblestone-like, without spines or papillae. At higher magnification the oral opening is seen
in the retractil tip of the forebody. Sensory papillae were not observed around the oral
opening. The large hindbody has two symmetrical rounded lobes on the anterior end, forming
a groove, from which emerges the elongated forebody; the posterior third of the hindbody is
curved ventrally forming a somewhat pointed tail. The hindbody measure 3.4-10.2 mm in
length by 0.9-3.1mm largest widht. The dorsal surface is more prominent than the ventral,
forming a cover with tegumental transversal striations. There are no spines or papillae on
either the ventral or the dorsal tegumentary surface of the hindbbody, which presents the same
wrinkled cobblestone-like appearance (Kohn et al. 2001)
8. Anisakisasis
8.1. Aetiology: Anisakis simplex and Hysterothylacium cornutum (Williams and BunkleyWilliams 1996).
8.2. Epizootiyology: The definitive hosts of these parasites are marine mammals. Many other
species of fish can act as intermediate hosts.
8.3. Pathogenicity: The small third-stage larvae are found encapsulated in the peritoneal
mesenteries and, sometimes, the liver. If the fish are not quickly eviscerated it is possible for
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

the larvae to migrate to the abdominal muscles. Anisakis sp. infections are of importance
because they can potentially infect humans.
8.4. Symptoms: The infections are covert.
8.5. Diagnosis: The presence of tightly coiled, encapsulated larval nematodes in the
mesenteries of tunas is suggestive of anisakid nematode infection, but definitive identification
of the larvae can be difficult.
8.6. Treatment and Prophylaxis: Human infection can be prevented by rapid evisceration of
the fish and/or cooking of the flesh. In most instances tuna destined for sashimi are
eviscerated soon after capture. There is no practicable treatment.
9. Copepodiasis
9.1. Aetiology: C. elongatus, Euryphros brachypterus, Penella filosa and Pseudocycnus
appendiculatus
Overall length of female of Pseudocycnus appendiculatus is 13.7 mm-15.6 mm. Anterior
quarter of ventral length bright red, rest of body yellowbrown, the red pigment persistent in
alcohol for at least several months. Cephalothorax subovate, (1.2 mm-1.3 mm x 1.2 mm),
greatest width a little posterior to midpaint. Second thoracic segment subovate, length/width
(0.6 mm-0.8 mm x 1.1 mm). Third thoracic segment, length/width (0.5 mm-0.6 mm x 1.0
mm-1.2 mm). Fourth thoracic segment subovate, length/width (1.7 mm x 1.2 mm-1.3 mm).
Trunk, excluding the anterior swollen portion, cylindrical, length more than 6 times width (6.6
mm-7.9 mm x 1.0 mm-1.1 mm), lateral margins parallel with 2 plate-like subsemicircular
dorsal projections, % trunk width, length % width, carried laterally on posterior margin.
Abdomen, length % width (0.6 mm-0.7 mm x 0.9 mm-l.0 mm), caudal laminae borne laterally
on posterior margin of abdomen, length 12 times basal width (3.4 mm-3.8 mm x 0.3 mm-0.4
mm). Egg strings extending from posterior margin of trunk, beneath platelike projections
dorsal to abdomen, present on only 1 specimen, as long as body, eggs uniserial, length 13.7
mm, posterior of egg strings empty of eggs in this individual (Hewitt 1969, Purivirojkul
2011).
9.2. Epizootiyology: As C. elongatus and Penella filosa have multiple hosts, tuna may become
infected from a variety of sources. However, Euryphorus brachypterus is almost genus
specific for Thunnus spp. (Williams and Bunkley-Williams 1996). In the case of C. elongatus
infecting captive southern bluefin tuna, capture trauma and high stocking densities are
believed to predispose to heavy infections (Rough et al. 1999).
9.3. Pathogenicity: Lesions because of the copepods are related to their grazing behaviour (C.
elongatus) or the damage caused by their attachment to the host (Euryphorus brachypterus and
Penella filosa). Opportunistic infections of the bacterium Aeromonas sp. have also been
reported in association with louse-associated damage to tuna eyes (Munday et al. 2003).
9.4. Symptoms: C. elongatus grazes on the integument of southern bluefin tuna and may
produce grazing trails including over ocular tissues. Damage to the eye results in keratitis,
panopthalmitis, cataract formation and blindness, leading to significant production losses
(Rough 2000, Munday et al. 2003). Very heavy infections of Euryphorus brachypterus have
been reported in northern bluefin tuna in which the pseudobranch has been carpeted with the
parasite leading to ulceration and bleeding (Rough et al. 1999). Similar, but less severe lesions
may be present on the gills and skin. The very large copepod Penella filosa penetrates into the
47

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

muscles of a number of tuna species. It has been reported to cause the fish considerable
discomfort (Williams and Bunkley-Williams 1996).
9.5. Diagnosis: Experts can make a presumptive diagnosis of these copepod infections based
on the morphology of the parasites and the types of lesions induced by their activities.
However, definitive diagnosis is only possible by a scientist skilled in identifying the
parasites.
9.6. Prophylaxis: Trauma may predispose to C. elongatus infections then reduction of damage
because of capture, towing and harvesting should simultaneously reduce the level of
infestation/damage caused by this copepod. Additionally, as this parasite and Penella filosa
are carried by other species of fish, it would be appropriate to keep other forms of aquaculture
separate from tuna farms (Rough et al. 1999).
9.7. Treatment: Although a number of therapeutants are capable of killing copepod parasites
(Lester and Roubal 1999) it is impracticable to use these agents under current tuna
aquaculture conditions. In addition, at the present level of loss of production, such treatments
would be uneconomical.
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28:131-134.

Parasitic Diseases of Trout and Their Controls
in Sustainable Development of Aquculture: Platyhelminthes
Erol Tokşen1, Erkin Koyuncu2
1Ege University, Fisheries Faculty, Department of Fish Diseases, Bornova 35100 İzmir,
Turkey
2Mersin University, Fisheries Faculty, Department of Fish Diseases,Yenişehir 33169 Mersin,
Turkey
E-mails: erol.toksen@ege.edu.tr, ekoyuncu@mersin.edu.tr

Abstract
Aquaculture is an important food-producing sector. It provides much needed protein,
employment, income and livelihoods support to many people in the world and this is
especially true in most developing countries. A significant challenge to the expansion of
aquaculture production is the outbreak of disease. Potential economic losses from disease
outbreaks are significant, and can affect the survival of the industry. Fish diseases were
caused by enfectious agents as parasite, bacteria, virus and fungus. Gyrodactylus spp.,
Tetraonchus spp., Discootyle spp., Cyathocephalsus spp., Proteocephalus spp., Eubothrium
spp., Diphyllobothrium spp., Trianophorus spp., Crepidostomum spp., Phyllodistomum spp.,
Sanguinicola spp., Diplostomum spp., Tylodelphys spp., Apatemon spp. are observed in
rainbow trout. Gyrodactylus salaris caused the mortality up to 95 % in salmon population in
some rivers in Norvay. So, G. salaris is a disease between compulsory notifiable diseases in
EU. However, such a pathogen monogenean enfestations have not been appeared in Turkey,
First record of Crepidostomum farionis was carried out in rainbow trout in Elazığ from
Turkey. The present work aim to the parasitic diseases of freshwater trout caused by phylum
50

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                <text>Parasitic Diseases And Their Controls In Sustainable Development Of Aquculture Of  Bluefin Tuna (Thunnus Thynnus)</text>
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                <text>In the last decades Turkish and Mediterranean mariculture has focused its production mainly  on two species, gilthead sea bream (Sparus aurata L.) and sea bass (Dicentrarchus labrax L.).However, due to its high commercial value bluefin tuna (Thunnus thynnus) has been  considered as an alternative aquaculture target. The culture of bluefin tuna has started by  private sector in 2001 in Turkey. The catched fishes are fed by frozen herring, sardina,  mackerel between May and June and after fattened, they are sold. Total feeding time in  Turkey 4 to 10 months like other countries which are culturing bluefin tuna. As an developing  sector in Turkey and in the world, blue fin tuna farming require to further studies on larvae  production, feed investigations and diseases occur on the fish. According to studies conducted  to date, 28 species were found in Pylums Ciliphora, Myxozoa, Platyhelmintes, Aschelmintes  and Arthropoda. The present work aim to reveal diseases of bluefish tuna caused by parasites,  how they are transmitted, which effects they have on tuna fish, how they could be diagnosed,  and how they could be controlled and treated.  Keywords: Bluefin tuna, disease, parasite, diagnosis, treatment.</text>
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                    <text>3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Santer R.M. &amp; Greer-Walker M. (1980) Morphological studies on the ventricle of teleost and
elasmobranch hearts. Journal of Zoology (London) 190, 259–272.
Smith J.W. (1997) The blood flukes (Digenea: Sanguinicolidae and Spirorchidae) of coldblooded vertebrates: Part 2. Helminthological Abstracts 66, 329–344.
Watts, M. (1995) The development of a fluorescent antibody stain to identify a Uronenla sp.
(Ciliophora: Scuticociliatida) implicated in fatal encephalitis in farmed tuna (Thunnus
maccoyii). Honours thesis, University of Tasmania, Launceston.
Williams E.H. &amp; Bunkley-Williams L. (1996) Parasites of Offshore Big Game Fishes of
Puerto Rico and the Western Atlantic. Puerto Rico Department of Natural Environmental
Resources and the University of Puerto Rico, Mayaguez.
Yoshinaga T, Nakazoe J (1993) Isolation and in vitro cultivation of an unidentified ciliate
causing scuticociliosis in Japanese flounder (Paralichthys olivaceus). Gyobyo Kenkyu
28:131-134.

Parasitic Diseases of Trout and Their Controls
in Sustainable Development of Aquculture: Platyhelminthes
Erol Tokşen1, Erkin Koyuncu2
1Ege University, Fisheries Faculty, Department of Fish Diseases, Bornova 35100 İzmir,
Turkey
2Mersin University, Fisheries Faculty, Department of Fish Diseases,Yenişehir 33169 Mersin,
Turkey
E-mails: erol.toksen@ege.edu.tr, ekoyuncu@mersin.edu.tr

Abstract
Aquaculture is an important food-producing sector. It provides much needed protein,
employment, income and livelihoods support to many people in the world and this is
especially true in most developing countries. A significant challenge to the expansion of
aquaculture production is the outbreak of disease. Potential economic losses from disease
outbreaks are significant, and can affect the survival of the industry. Fish diseases were
caused by enfectious agents as parasite, bacteria, virus and fungus. Gyrodactylus spp.,
Tetraonchus spp., Discootyle spp., Cyathocephalsus spp., Proteocephalus spp., Eubothrium
spp., Diphyllobothrium spp., Trianophorus spp., Crepidostomum spp., Phyllodistomum spp.,
Sanguinicola spp., Diplostomum spp., Tylodelphys spp., Apatemon spp. are observed in
rainbow trout. Gyrodactylus salaris caused the mortality up to 95 % in salmon population in
some rivers in Norvay. So, G. salaris is a disease between compulsory notifiable diseases in
EU. However, such a pathogen monogenean enfestations have not been appeared in Turkey,
First record of Crepidostomum farionis was carried out in rainbow trout in Elazığ from
Turkey. The present work aim to the parasitic diseases of freshwater trout caused by phylum
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Plathyhelmintes, how they are transmitted, which effects they have on trouts, how they could
be diagnosed, and how they could be controlled and treated.
Keywords: Trout, disease, parasite, platyhelminthes, diagnosis, treatment
1.INTRODUCTION
Turkey has rich inland water sources, about 200 natural lakes, about 750 artificial
lakes or ponds, about 193 reservoirs, 33 rivers and streams of 177.714 km length and 8.333
km of coastal strips. Aquaculture sector in Turkey is new when compared with European
countries. The first fish farm was established as a rainbow trout farm in 1970s. The following
years, new fish farms have been established year by year. The main fish species cultured in
Turkey are Carp (Cyprinus carpio), Rainbow trout (Oncorhynchus mykiss), Atlantic salmon
(Salmo salar), Gilthead sea bream (Sparus aurata), European sea bass (Dicentrarchus labrax),
Bluefin tuna (Thunnus thynnus), Black sea turbot (Psetta maxima), Mediterranean mussel
(Mytilus galloprovincialis) and Shrimp (Penaeidae spp). Aquaculture production of Turkey
has grown steadily over the years from 5782 tonnes in 1990 to 63 000 tonnes in 1999 and to
152 186 tonnes in 2008. Recently, it is shown in Table.1 that trout pruducts have reached to
68,649 tons in Turkey (TUIK 2012).

Type of fish

2006

2007

2008

2009

2010

Inland water Trout 56 026

58 433

65 928

75 657

78 165

Marine Trout

1 633

2 740

2 721

5 229

7 079

Total

57 659

61 173

68 649

80 886

85 244

Table.1. Aquaculture production of Turkey (Ton) (TUIK 2012)
The intensification of aquaculture and globalization of the seafood trade have led to
remarkable development in the aqauculture industry. The industry has been plagued with
disease problems caused by viral, bacterial, fungal and parasitic pathogens. In recent years,
disease outbreaks are becoming more frequent in he aquaculture and associated morbidity and
mortality have caused substantial economic losses. Health problems have two fiscal
consequences on the industry: loss of productivity due to animal mortality and morbidity, and
loss of trade due to food safety issues. Thus, disease is undoubtedly one of the major
constraints to production, profitability and sustainability of the aquaculture industry. Parasites
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

in fish have become increasingly visible during the latest decades in connection with the
development of aquacultural industries in the world. Thus, focus has been placed on parasitic
infections in these enterprises and their economic and ecological impact.
Diseases problem caused by parasitic organisms are the main threat to further increase of the
industry. There are various parasites caused the diseases on the trout. This study consist of
crustaceans parasites. This research presents the individual parasites types producing
problems in sea bream and sea bass. Each section is presented with 1. aetiyology, the parasitic
organism responsible for the disease, 2. epizootiology, the transmission of the diseases and
life cycle of the parasite, 3. pathogenicity, how the parasite produces diseases in the fish, 4.
symptoms, clinical signs of the diseases, 5. diagnose, how the infection can be identificated,
6. treatment, how the infection can be controlled.
The Plathelminthes typically are slightly dorso-ventrally flatted, witth a few
exceptions hermophroditic worms without an anus and some of them without a mouth and
intestine. Their surface layer is a tegument which is made of a syncytium. This Phylum
comprise monogeneans, digeneans and cestodes
Monogeneans are flatworms with representatives in freshwater, brackish and marine
habitatts. The most of species are ectoparasitic and they all have a direct life cycle. A few
species have adopted an endoparasitic life. They have a characteristic posterior attachment
organ called an opisthaptor. This organ is equipped with sclerotiinized structures as large
hooks, clamps and marginal hooklets. Also the fore part of the parasite has adhesive pads.
Using these adhesive structures he flatworm can move in a leech-like manner on the host.
2. Tetraonchosis:
2.1. Aetiology: Tetraonchus alaskensis, T. awakurai. T. oncorhynchi
The lenght of the T. awakurai is 650-1080 µm and width is 250-415 µm (Ogawa and Egusa
1978).
2.2. Epizootiology: Tha adult parasite attaches to the gill epithelium with its two pairs of
anchors and their associated marginal hooklets, then produce eggs. These are liberated to the
aquatic enviroment where they develop and hatch. The ciliated larva called oncomiracidium
seeks the its host, attaches, sheds the ciliated cells and moves to the gill filaments. Depending
on the ambient temperature the post larva develops to the adult stage on the gills (Buchmann
and Bresciani 2001). Oncomiracidia hatch from embryonated eggs and transmission occurs
when the temperature is above 10 °C (Ogawa and Egusa 1985).
2.3.Pathogenicity: Heavy infections mat elicit inflamatorry reactions in the gills, hyperplasia
of epithelial and mucous cella and haemorrhages.
2.4. Symtomps: Parasite cause hyperventilation, lethargy and mortality.
2.5. Diagnosis: Detection of the parasite on the gills of the host. The parasite has four eye
spots, four large hooks in the opisthahaptor.
2.6. Prophylaxis: Quarantine measures, mechanical filtration of fish tank water to remove
oncomiracidia.
2.7. Treatment: Formalin, mebendazole and praziquantel may have effect (Buchmann and
Bresciani 2001).
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

3. Gyrodactylosis:
3.1. Aetiology: Gyrodactylus salmonis, G. salaris, G. derjavini, G. truttae, G. colemanensis,
Gyrodactylus elegans, G. cobitis. G. salmonis measures 0.5 mm in length and 0.15 mm in
width. The hamuli are stout and 53-65 um long.
3.2. Epizotiyology: They are vivipourus hermophroditic parasites. The female parasite give
birth to an almost fully developed daughter which already has a growing embryo in its uterus.
The parasites attcches to thebody surface, fins, buccal cavity. At a farm nets and buckets are
potential vehicles of transmission. Water-borne transmission occurs in spring when melting
ice frequently causes flooding between holding units. Shipment of fish from other facilities
are also potential sources of infeciton and so is the water supply of the farm.
3.3. Pathogenicity: Due to penetration of the epithelium by the marginal hooklets and the
feeding activitiy the fish may suffer from osmoregulatory distress. Opportunistic infections
may arise in the injured epidermis.
3.4. Symtomps: Heavily infected fish with parasite are cachexic, lethargic and overall dark in
clour (Cone and Cusack 1988).
3.5. Diagnosis: Microscopic examination of slides with the parasite shows the approximately
0.5 mm platyhelminth without eyespot but with two large ventral hamuli, connected by a
dorsal and ventral bar and 16 marginal hooklets. The shape and size of the hamuli and
hooklets are species characteristic.
3.6. Prophylaxis: Quarantine measures. Strict prevention of the introduction of specific
gyrodactylids may be necesssary in farms with susceptible fish species.
3.7. Treatment: 4.5-5 % solution of NaCl for 1.5 to 2.5 min and then transfer immediately to
fresh runnig water. A second bath may be necessary after several days (Cone 1991).
Triclabendazole and nitroscanate are effective at dosages 40 g/kg of feed for 10 d and 0.6 g/kg
of feed for 1 d, respectively (Tojo and Santamarina 1988). Formalin, copper sulphate,
hydrogen peroxide, sodium per carbonate, mebendazole, praziquantel may use.
4. Discocotylosis:
4.1. Aetiology: Discocotyle sagittata, D. ohridana.
D. sagittata is up to 12 mm length, 3-4 mm width. The rectangular opisthaptor is equipped
with four pairs of clamps for attachment to gill.
4.2. Epizotiyology: Sanguinivorous polyopisthocotylean monogenean occur on the gills of
freshwater salmonid fishes, with an extensive geographical distribution in the Northern
Hemisphere. It occurs at low intensities in the wild (Valtonen et al. 1990). Eggs are produced
by the harmaphroditic worm. The egg embryonate and hatch. The larva seek the host and
develops into the adult stage.
4.3. Pathogenicity: The parasite feed on host blood and may cause anameia. Inflammatory
reactions may impair respiration. Heavy infection by D. sagittata can result in deleterious
effects on the host, especially in the context of fish farms. Rainbow trout reared in the Isle of
Man have been shown to harbourhigh parasite burdens (˃1000/fish) and exibit high mortality
rates due toparasite-induced anaemia (Gannicott 1997).
4.4. Symtomps: Anameia, morbiditiy, hyperventilation.
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4.5. Diagnosis: Detection of the up to 12 mm long worm on the gills of the host. They are
charecterized by the clamps on the opisthaptor.
4.6. Prophylaxis: Quarantine measures should be applicated.
4.7. Treatment: Praziquantel (Rubio-Godoy and Tinsley 2004)
Digeneans are heteroxenous and their adult stages are parasitic in vertebrates. They
need more than one host in their life cycles. Molluscs serve as first intermediate host.
Bivalves are also the fisrt intermediate host of Fellodistomatidae, Gorgoderidae
(Phyllodistomum) and Allocreadiidae (Crepidostomum). Adult digeneans usually have a
dorsoventrally flattened oval body with a smooth, spiny or corrugated surface, a sucker
around the anteroventral mouth and an additional ventral sucker, or acetabulum. Both suckers
are used for attachment and locomotion (Paperna 2006).
5. Crepidostomosis:
5.1. Aetiology: Crepidistomum farionis, C. metoecus
5.2. Epizotiyology: The first intermediate hosts in the life history of this trematode are
Pisidium amnicum and Sphaerium corneum. Although the latter is unusual, the second
intermediate host is the larva of the mayfly, Ephemera danica. There are two generations of
rediae, the first gives rise to daughter rediae, which in turn produce cercariae. Final host is
trout (Brown, 2009).
5.3. Pathogenicity. The parasites cause swelling of the intestine. Inflammation of the intestinal
wall may cause malabsorption. Parasite reduce the hemoglobin and hematocrit levels in the
fish (Klein et al. 1969).
5.4. Symtomps: Inflammatory state of intestinal wall, haemorrhages.
5.5. Diagnosis: Detection of parasite in the intestine. The charecteristic cephalic lobes and the
two suckers are found in both species.
5.6. Prophylaxis: Removal of intermediate host.
5.7. Treatment: Praziquantel is liable to have effect when used in feed oral treatmenta.
Articles
6. Phyllodistomosis:
6.1. Aetiology: Phyllodistomum simile
6.2. Epizotiyology: Brown trout, salmon parr. Large numbers of Phyllodistomum simile were
found in the urinary bladder of the trout from the River Teify. There was no evidence of a
seasonal variation in the occurrence of P. simile in trout aged two years or more.Trout aged
four or more years were more heavily infested than young trout and there was no indication of
the development of a total immunity with increase in the age of fishes. The life history of P.
simile was elucidated under experimental conditions by feeding the trout precocious
metacercariae present in daughter sporocysts obtained from the gill lamellae and epibranchial
cavity of the freshwater bi-valve, Sphaerium corneum (Thomas 1958).
6.3. Pathogenicity: Extension of urethrae.
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6.4. Symtomps: Not clear.
6.5. Diagnosis: Detection of the parasites in the urethrae or bladder.
6.6. Prophylaxis: Removal intermediate hosts.
6.7. Treatment: Praziquantel may have effect (Buchmann and Bresciani 2001).
7. Nanophyetosis:
7.1. Aetiology: Nanophyetus salmincola is a digenic trematode, which means that it is an
unsegmented worm that is flattened dorsoventrally. Adult worms alternate shape from “a
sphere to a long blunt rod. The worms are 0.8 to 1.1mm long and 0.3 to 0.5 mm wide and are
hermaphroditic, having both male and female reproductive organs in the same organism.
(Bennington and Pratt 1960).
7.2. Epizzotiyology: The life cycle of the N. salmincola requires three hosts. The first
intermediate host is an Oxytrema silicula stream snail. The second intermediate host is a
salmonid fish, though some non-salmonid fishes also play a role. Lastly, the definitive host is
most commonly a canid, though many other mammals are also definitive hosts, including
humans. Transmission of N. salmincola to the definitive host occurs upon ingestion of
parasite-infected fish (Bennington and Pratt 1960).
7.3.Pathogenicity: Cercariae invading fish fry may kill the fish probably due toimpairment of
the osmoregulatory system. The parasite can transmit bacterial infection (Neorickettsia
helminthoeca) to the dog.
7.4. Symtomps: Lethargy, morbidity, mortality of fish.
7.5. Diagnosis: Detection of metaacercariae in fish tissue. These are pyriform, unspined, large
oral sucker of same size as ventral sucker, short oesaphagus.
7.6. Prophylaxis: Removal of intermediate snail hosts.
7.7. Treatment: Praziquantel may have effect in the early stage of infection but this has not
been documented (Buchmann and Bresciani 2001).
8. Sanguinicolosis:
8.1. Aetiology: Sanguinicola alsea, S. davisi, S. fontinalis, S. idahoensis, S. klamathensis.
Sanguinicola fontinalis body very flat, elongate when fixed relaxed, colourless, translucent,
0.90 mm long, 0.21 mm wide after hot fixation, staining and mounting (Hoffman et al. 1985).
8.2. Epizotiyology: The adult parasite lives blood vessels of the fish. Eggs are produced wich
hatch to release the first larval stage called the miracidum.. These larvae from the eggs pass
out of the gills into the pond water. In the pond miracidia infect the intermediate host as snails
of the genus Oxytrema or Fluminicola. In the intermediate host sporocysts are developed and
release cercariae which are infective for the fish. Then parasite penetrate into the blood vessel
and develop there.
8.3. Pathogenicity: The release of eggs and penetrating miracidia mat elicit pathological
reactions in the gill and blood vessels.
8.4. Symtomps: Branchitis (diseased gill), swollaen kidney, mortality, morbidity, lowered
haematocrit.
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8.5. Diagnosis: Detection of parasite (length 0.5-0.9 mm) in the blood vessels (branchial,
renal, cardiac vessels). The adultfluke is lanceolate withour suckers and a narrow
oesophaagus. Eggs are thin-shelled.
8.6. Prophylaxis: Removal of snails from ponds and the environment. Filtration of pond water
to clear infective cercariae.
8.7. Treatment: Praziquantel may have a effect but this has not been documented (Buchmann
and Bresciani 2001).
9. Diplostomosis:
9.1. Aetiology: Larvae (metacercariae) of Diplostomum spathaceum (Diplostomatidae,
Digenea). Body length of cercariae is 160-260 µm and the bifurcated tail stem is as long as
the body. The established metacercariae are flattened, about 400 µm in length, and possess
two suckers and an attachment organ (Hoglund 1999).
9.2. Epizotiyology: The life cycle involves pulmonate snails as first intermediate hosts, fish as
second intermediate hosts, and piscivorous birds as final hosts harbouring the adult worms.
Fish are infected with cercariae. The adult parasite in the small intestine of a fish eating bird
releases eggs. These pass with the host faeces to the aquatic environment where they hatch
and release free swimming miracidia. These infect the first intermediate host, a pulmonate
snail, Lymnaea pereger or L. stagnalis. In the snails a new larval stage, the sporocyst,
develops. Tail bearing larvae, cercariae, in turn appear in the sporocysts and are liberated to
the water where tthey seek a fish host. When attached to the skin the tail is lost and the
invasion process is initiated. Following penetration of the fish skin, the larva migrates
through the lymphatic ducts to the final locality (the eye) in the fish. In the eye the larva
develops into the metacercarial stage (Buchmann and Bresciani 2001).
9.3. Pathogenicity: The free-living feeding metacercariae lie in the cortex of the lens. Rupture
of the lens and proliferating lens epithelium are sometimes observed at the site of parasite
entry (Hoglund 1999). Cercarial penetration of skin may compromise osmoregulation
(Buchmann and Bresciani 2001).
9.4. Symtomps: Infection may result in cataract formation within the lens, leading to partial or
total blindness. Starvation may cause a significant reduction in condition, or even death. After
penetration of the cercariae, lesions may form on the gills, body surface, and fins, which can
cause death, especially in young fish, at high infection rates (Hoglund, 1999).
9.5. Diagnosis: Detection of the metacercaria in the lens of the fish.
9.6. Prophylaxis: Removal of intermediate snail hosts. Preventing access of final host (fish
eating birds) because they release parasite eggs. Mechanical filtration of pond water may
reduce density of cercariae. Increrase of water flow in ponds may flush away the positevely
phototactic cercariae.
9.7. Treatment: Praziquantel (330 mg/kg) has been used orally in feed (Buchmann and
Bresciani 2001).

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10. Apatemonosis:
10.1. Aetiology: Apatemon gracilis
10.2. Epizotiyology: Adult trematodes live in birds. First intermediate host is a freswater
snail, the trout is the second intermediate with infective metracercariae. When the fish-eating
birds eats the fish it will result in maturation of tehe metacercaria into the adult stage in the
intestine of the bird.
10.3.Pathogenicity: The presence of metaccercariae in host tissue produce inflammatory
reactions which may affect physiological function of the organ. Trout infection with
Apatemon gracilis resulted in fibrogranulomatosis of the epicardium and failure in in-vitro
pumping performance (Tort et al. 1987, Watson et al. 1992).
10.4. Symtomps: Heavy infections associated witth lethargic fish. Heart function may
decrease.
10.5. Diagnosis: Detection of metacercaria in the fish tissue.
10.6. Prophylaxis: Eradication of intermediate host snails in the culture system and
surroundeings. Prevention of bird entry.
10.7. Treatment: Anthelmintic such as praziquantel may have effect (Buchmann and Bresciani
2001).
11. Tylodelphysosis:
11.1. Aetiology: Tylodelphys clavata, metacercariae; body lenght is 0.28-0.69 mm, width is
0.8-0.21 mm. Diameter of oral sucker is 0.025–0.047 x 0.021–0.039 mm, ventral sucker is
0.016–0.029 x 0.020-0.029 mm. Brandes's organ 0.048-0.079 x 0.021–0.045 mm (Karatoy
and Soylu 2006).
11.2. Epizotiyology: The adult parasite in the small intestine of a fish-eating bird sheds
parasite eggs. these are delivered with the host faeces to the aquatic environment where they
hatch and release free awimminng miracida. The first intermediate host, a pulmonate
snail,become infected by penetration. In the snails a new larval stage, the sporocyst, develops.
Tail-bearing larvae, cercariae, in turn appear in the sporocyst and are liberated to the water
where they seek a fish host. When attached to the skinthe tail is lost and the invading process
is initiated. Following penetration of the fish skin, the larva migrates in the lymphatic ducts to
the final locality (the eye) in the fish. In the eye the larva develops into the metacercarial
stage.
11.3. Pathogenicity: The movements and excretion from the metracercaria may cause
disturbances of the eye function.
11.4. Symtomps: Normally metacercariae do not cause serious disease. However, penetration
of cercaria into small fish ma elicit mortality.
11.5. Diagnosis: Detection of metacercaria in the vitreous humour of the fish.
11.6. Prophylaxis: Removal of intermaediaate snail hosts. Preventing access of final fisheating hosts releasing parasite eggs. Mechanical filtration of pond water may reduce density
of cercariae. Increase of water flow in ponds may flush away the positively phototactic
cercariae.
11.7. Treatment: Praziquantel (330 mg/kg) may be used orally in feed (Buchmann and
Bresciani 2001).
57

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

The cestoda or tapeworms are exclusively endoparasitc and hermoaphroditic with no
asexual propagation in the intermediate host. Cestodes are oviparaous and have complicated
life cycles which in many cases include free-swimming ciliated larvae called coracidia. Other
cestode eggs infectt the host by hatching after ingestion. They have no mouth nor intestine.
They feed by absorbing of substances through their tegument.
12. Cyathocephalosis
12.1. Aetiology: Cyathocephalus truncatus
12.2. Epizootiyology: Hexacanth embryos of C. truncatus were found to develop optimally in
eggs cultured at between 15 °C and 20 °C for about 25 days. Gammarus pulex became
infected only by swallowing egg capsules a containing hexacanth embryos fed to them. The
young developing embryo grows over a period of about 10 weeks to the infective procercoid
stage in the body cavity of the amphipod. In fish, the tapeworm forms an attachment in the
distal end of a pyloric caecum 3 days after infection and matures in 8-10 days with production
of eggs. By the 15th day the attachment to host tissue has become so firm that it is impossible
to separate the worm from it. The tapeworm's hold on the caecal wall is probably achieved by
the sucking effect of the funnel-shaped scolex supplemented by the spike-like microtriches of
the inner scolex surface (Okaka 1984).
12.3. Pathogenicity: In heavily infected fish, the entire region of the pyloric caeca becomes
fused into a single mass which may additionally fuse with the abdominal body wall
immediately adjacent to it. In some cases, tapeworms were seen to have penetrated through
the caecal wall and were attached directly to the muscles of the abdominal wall with the
strobila still within the caecum. In other cases the whole tapeworm had moved through the
perforated distal tip of the caecum and was found free in the body cavity. Worms could also
be discharged into the intestinal tract with the detached distal, end of the caecum still held in
the soolex as described previously. Caeca with perforated tips are common in heavily infected
fish.
12.4. Symtomps: Heavily infected fish are often pale in colour, appearing very anaemic and
showed blister-like pustules on the skin of the abdomen. Light infections do not elicit
symptoms but mass mortalities in association with heavy infections have beeen described
(Okaka 1984).
12.5.Diagnosis: Unsegmented cestode with a maximum length of 5 cm. The scolex is cupshaped.
12.6. Prophylaxis: Removal of intermaediate snail hosts.
12.7. Treatment: Praziquantel, mebendazole or albendazole may have effect due to their
anticestodal activity (Buchmann and Bresciani 2001).
13. Proteocephalosis
13.1. Aetiology: Procephalus longicollis
13.2. Epizootiyology: The adult hermaphroditic cestode produce eggs which are liberated to
the water. Following hatching the larava (coracidium) will be ingested by a copepode which
in turn becomes infected with the procercoid stage. When the fish eats the copepod this
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

crustacean will get deigested whereas the cestode larva develops into the adult stage in the
fish intestine. Adult cestodes are usually located in the anterior part of the intestine or caeca,
while juveniles may be found throughout the intestine (Scholz 1999). Parasite abundance is
highest in the period mid June - mid July
13.3. Pathogenicity: Mainly due to absorption of nutritive substances in the host intestine.
Inflammation of intestinalepitthelium due to cestode attachment.
13.4. Symtomps: It has been suggested that infections with P. longicollis reduce the growth of
rainbow trout (Priemer and Goltz 1986, Engelhardt et al. 1988).
13.5. Diagnosis: Detection in intestine of cestodes with four suckers.
13.6. Prophylaxis: Removal of intermaediate copepod hosts.
13.7. Treatment: Praziquantel (Buchmann and Bresciani 2001).
14. Eubothriosis
14.1. Aetiology: Eubothrium crassum E. salvelini
14.2. Epizootiyology: Transmission of E. crassum occurs when fish ingest infected copepods,
and the first site of E. crassum is either the stomach or intestine, where digestion of the
copepod happens. Growth only commences when a plerocerciform worm moves into a
caecum, and larger worms are more likely to be found in anterior caeca, the preferred site of
E. crassum (Kennedy 1996). The increase in prevalence and abundance of E. crassum occur
from late March to mid May.
14.3. Pathogenicity: The presence of the cup-shaped scolex in the tip of the pyloric caecum
induces an extensive ball-shaped tissue reaction.
14.4. Symtomps: infection by E. crassum may have a negative effect on host condition factor..
Parasite cause direct mortalities among the heavily infected fish, and indirect mortalities by
predation from reduced growth, poor swimming performance and aberrant behaviour. The
negative growth effects highlighted are considered caused either by poor host nutrition due to
demands of the parasite or from reduced food gathering capabilities (Smith 1973).
14.5. Diagnosis: Detection of cestodes in the pyloric caeca and intestine.
14.6. Prophylaxis: Removal of intermaediate copepos hosts. Filtration of water.
14.7. Treatment: Di-n-butyl tin oxide 80-160 mg/kg three days (Mitchell 1993)
15. Diphyllobothriosis
15.1. Aetiology: Diphyllobothrium is a genus of tapeworm which can cause
Diphyllobothriasis in humans through consumption of raw or undercooked fish. The principal
species causing diphyllobothriosis is Diphyllobothrium latum, known as the broad or fish
tapeworm, or broad fish tapeworm. D. latum is a pseudophyllid cestode that infects fish and
mammals. D. latum is native to Scandinavia, western Russia, and the Baltics, though it is now
also present in North America, especially the Pacific Northwest. In Far East Russia, D.
klebanovskii, having Pacific salmon as its second intermediate host, was identified (Muratov
and Posokhov 1988).

59

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

15.2. Epizootiyology: Adult tapeworms may infect humans, canids, felines, bears, pinnipeds,
and mustelids. Immature eggs are passed in feaces of the mammal host. After ingestion by a
suitable freshwater crustacean such as a copepod (the first intermediate host), the coracidia
develop into procercoid larvae. Following ingestion of the copepod by a suitable second
intermediate host, typically a minnow or other small freshwater fish, the procercoid larvae are
released from the crustacean and migrate into the fish's flesh where they develop into a
plerocercoid larvae. The plerocercoid larvae are the infective stage for the definitive host.
15.3. Pathogenicity: Inflammatory reactions in the affected tissue.
15.4. Symtomps: Clinical signs of diphyllobothriasis are generally mild, and can include
diarrhea, abdominal pain, vomiting, weight loss, fatigue, constipation and discomfort in
human. Approximately four out of five cases are asymptomatic and may go many years
without being detected (Scholz et al. 2009). In a small number of cases, this leads to severe
vitamin B12 deficiency due to the parasite absorbing 80% or more of the host’s B12 intake,
and a megaloblastic anemia indistinguishable from pernicious anemia (David and William
2006).
15.5. Diagnosis: Detection of pleurocercoids in the fish viscera of flesh.
15.6. Prophylaxis: Contamination of the environment with worm eggs should be prevented.
15.7. Treatment: Praziquantel may have effect (Buchmann and Bresciani 2001)
16. Triaenophorosis
16.1. Aetiology: Triaenophorus crassus, T. nodulosus
16.2. Epizootiyology: Adults of T. crassus and T. nodulosus occur in the intestine of the pike,
Esox lucius L. The first intermediate hosts of Triaenophorus spp. are cyclopoid copepods and
in European waters Cyclops vicinus and C. strenuus are the most important first hosts of T.
crassus (Kuperman 1973). Plerocercoids of T. crassus occur in the musculature of the fish
host, and in wild populations of salmonids and coregonids may cause loss of quality in fish
products. Infection first occurs in early June when the trout fry have been moved into the
cages (Bauer and Solomatova 1984).
16.3. Pathogenicity: Severe inflamatory reactions in muscles due to infectins with T. crassus.
The plerocercoids may exert pressure on organs and thereby disturb normal physiological
function.
16.4. Symtomps: Parasite cause letthargy and mortality of fingerlings.
16.5. Diagnosis: Detection of plerocercoids in the organs or muscles of the intermaediate host.
The worms have four characteristic hooks shaped as three-toothed combs in the scolex.
16.6. Prophylaxis: Removal of intermaediate copepod hosts. Any pike in the surrondings
should be removed. Water filtration prevent to contaminate of copepods.
16.7. Treatment: Anticestodal drugs such as praziquantel mat have effect (Buchmann and
Bresciani 2001).

60

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REFERENCES:
Bauer, O.N. and Solomatova, V.P. (1984) The cestode Triaenophorus crassus (Pallas)
(Pseudophyllidea: Triaenophoridae) as a pathogen of cage-reared salmonids, Journal of Fish
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Bennington, E. and Pratt, I. (1960) The Life History of the Salmon-Poisoning Fluke,
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Brown, F.J. (2009) On Crepidostomum farionis O. F. Müll. (Stephanophiala laureata Zeder), a
Distome Parasite of the Trout and Grayling. I. The Life History, Parasitology, 19, 86-99.
Buchmann, K. and Bresciani, J. (2001) An Introduction to Parasitic Diseases of Freshwater
Trout. DSR Publishers, 76 p.
Cone, D.K. (1991) "Monogenea (Phylum Platyhelminthes)" in: P.T.K. Woo (ed), Fish
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David T. John &amp; William A. Petri (2006) The blood- and tissue-dwelling nematodes. Markell
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Dörücü, M. (2000) Occurrence and first record of Crepidostomum farionis, Müller 1784
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Turkey, Fırat Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 12 (1), 323-327.
Engelhardt, A., Mirle, C., Granitza, I. and Petermann, H. (1988) Studies into Proteocephalus
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Hoglund, J. (1999) Proceedings of International Council for the Exploration of the Sea. ICES
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Hoffman, G. L., Fried, B. and Harvey J. E. (1985) Sanguiiucola fonthialis sp. nov. (Digenea:
Sanguinicolidae): a blood parasite of brook trout, Salveliniis fontinalis (Mitchill), and
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Linnaeus, 1758)’nın Metazoan Parazitleri, Türkiye Parazitoloji Dergisi, 30 (3): 233-238.
Kennedy CR (1996) Establishment, survival and site selection of the cestode Eubothrium
crassum in brown trout, Salmo trutta. Parasitology, 112, 347-355.
Klein, W.D., Olsen, O.W. and Bowden, D.C. (1969) Effects of Intestinal Fluke,
Crepidostomum farionis, on Rainbow Trout, Salmo gairdnerii, Transactions of the American
Fisheries Society; 98, 1-6.
Kuperman B.I. (1973) Tapeworms of the Genus Triaenophorus, Parasites of Fish.
Experimental Systematics, Ecology. Nauka, Leningrad, 208 pp.
Mitchell, C.G. (1993) Eubothrium, The Scottish Office Agriculture and Fisheries Department,
Aquaculture Information Series, No:14.
Muratov, I.V. and Posokhov, P.S. (1988). Causative agent of human DiphyllobothriasisDiphyllobothrium klebanovskii sp. n.. Parazitologiia, 22, 165–70.
Ogawa, K. and Egusa, S. (1978) Two new species of the Genus Tetraonchus (Monogenea:
Tetraonchidae) from cultured Onchorhynchus masou, Bulletin of the Japanese Society of
Scientific Fisheries, 44, 305-312.
Ogawa, K. and Egusa, S. (1985) Tetraonchus infections of mosou salmon, Oncorhynchus
masou. Fish Pathology, 19, 215-223.
Okaka, C.E. (1984) Studies on the biology of Cyathocephalus truncatus (Pallas, 1781)
(Cestoda: Spathebothridea) in its fish and crustacean hosts. Thesis of doctorate, Department
of Pure and Applied Zoology, University of Leeds.
Paperna, I. and Dzikowski, R. (2006) "Digenea (Phylum Platyhelminthes)" in: P.T.K. Woo
(ed) Fish Diseases and Disorders, Volume 1: Protozoan and Metazoan Infections. Second
Edition, CAB International, Wallingford, U.K., 345-390.
Priemer, J. and Goltz, A. (1986) Proteocephalus exiguus (Cestoda) als parasit von Salmo
gairdneri (Pisces), Angewandte Parasitologie, 27, 157-168.
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Rubio-Godoy, M. and Tinsley, R.C. (2004) Immunity in rainbow trout, Oncorhynchus
mykiss, against the monogenean Discocotyle sagittata following primary infection,
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Rubio-Godoy, M. and Tinsley, R.C. (2008) Recruitment and effects of Discocotyle sagittata
(Monogenea) infection on farmed trout, Aquaculture, 274, 15–23.
Scholz, T. (1999) Life cycles of species of Proteocephalus, parasites of fishes in the Palearctic
Region: a review, Journal of Helminthology, 73, 1-19.
Scholz, T., Garcia, H.H., Kucha, R. and Wicht, B. (2009) Update on the Human Broad
Tapeworm (Genus Diphyllobothrium), Including Clinical Relevance. Clinical Microbiology
Reviews, 22, 146–160.
Smith, H.D. (1973) Observations on the cestode Eubothrium salvelini in juvenile sockeye
salmon (Oncorhynchus nerka) at Babine Lake, British Columbia, Journal of the Fisheries
Research Board of Canada, 30, 947-964.
Tojo, J.L. and Santamarina, M.T. (1988) Oral pharmacological treatments for parasitic
diseases of rainbow trout Oncorhynchus mykiss. II: Gyrodactylus sp., Diseases of Aquatic
Organisms, 33, 187-193.
Thomas, J.D. (1958) Studies On The Structure, Life History And Ecology Of The Trematode
Phyllodistomum Simile Nybelin, 1926 (Gorgoderidae: Gorgoderinae) From The Urinary
Bladder Of Brown Trout, Salmo Trutta L. Journal of Zoology, 130, 397–435.
Tort, L., Watson, J.J. and Priede, I.G. (1987) Changes in-vitro heart performance in rainbow
trout Salmo gairdneri Richardson, infected with Aphatemon gracilis (Digenea), Journal of
Fish Biology, 30, 341–347.
TUIK (2012) Fisheries Statistics, Ankara, Turkey.
Valtonen, E., Prost, M. and Rahkonen, R. (1990) Seasonality of two gill monogeneans from
two freshwater fish from an oligotrophic lake in Northeast Finland, International Journal for
Parasitology, 20, 101–107.
Watson, J.J., Pike, A.W. and Priede, I.G. (1992) Cardiac pathology associated with the
infection of Onchorhynchus mykiss Walbaum with Aphatemon gracilis Rud, Journal of Fish
Biology, 41: 163–167.
63

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                <text>Aquaculture is an important food-producing sector. It provides much needed protein,  employment, income and livelihoods support to many people in the world and this is  especially true in most developing countries. A significant challenge to the expansion of  aquaculture production is the outbreak of disease. Potential economic losses from disease  outbreaks are significant, and can affect the survival of the industry. Fish diseases were  caused by enfectious agents as parasite, bacteria, virus and fungus. Gyrodactylus spp.,  Tetraonchus spp., Discootyle spp., Cyathocephalsus spp., Proteocephalus spp., Eubothrium  spp., Diphyllobothrium spp., Trianophorus spp., Crepidostomum spp., Phyllodistomum spp.,  Sanguinicola spp., Diplostomum spp., Tylodelphys spp., Apatemon spp. are observed in  rainbow trout. Gyrodactylus salaris caused the mortality up to 95 % in salmon population in  some rivers in Norvay. So, G. salaris is a disease between compulsory notifiable diseases in  EU. However, such a pathogen monogenean enfestations have not been appeared in Turkey,  First record of Crepidostomum farionis was carried out in rainbow trout in Elazığ from  Turkey. The present work aim to the parasitic diseases of freshwater trout caused by phylum Plathyhelmintes, how they are transmitted, which effects they have on trouts, how they could  be diagnosed, and how they could be controlled and treated.  Keywords: Trout, disease, parasite, platyhelminthes, diagnosis, treatment</text>
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                    <text>3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

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The effects of work motivation in quality of work life and
A study on banking sector

Esra Zeynel
Süleyman Demirel University, Social Science Institution, Business Administration Doctoral
Programme, Isparta

Abstract
In terms of Suistainable development, in countries, the success and effectiveness of
instuitaions is quite important. One of the most important elements of providing success and
effectiveness of institutions that have the human resources is working effectively and
efficiently. In this respect, “motivation” is one of the most important factors in raising the
quality of work life in the institutions, employee productivity, the contribution of the
institution, and the sense of belonging in the institution. Motivation plays an important role in
business life. Motivation by definition means that the individual actuating motive. In the
business life, motivation, increase the concern of the employee's job, and as a result of raising
the quality of work life is an important factor that increase business efficiency. The higher
motivation increases the contribution of the institution by raising the productivity of the
employee. High rate of success of the organization by increasing employee motivation
provided by the institution will contribute to the country's economy. This aspects, in order to
take advantage of the present institutions active human sources,making efforts to enhance the
motivation of employees in achieving organizational effectiveness and efficiency and provide
the employees' job satisfaction. Happy and productive working lifes of employees in having a
sense of belonging in the institution can be used effectively in improving the motivation
factor is the result.This study measured the effect of bank employees, the care component of
motivation in raising the quality of work life survey was applied on the bank employees. In
this study, a survey was applied on bank employee in terms of assessment of the effect of
motivation in the raising the quality of work life of bank employee. Importance of motivation
arises in this sector. The higher motivation increases the productivity of employee,
278

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

institutional effectiveness and quality of working life. Motivating factors should be applied by
institutions.

1.INTRODUCTION
Motivation is a motive that the people to take to move.Motivationword for Englishthat means
of move was derived from the root of “mot” in Latin. In the pscyhology motivation is used for
as purposeful behaviors that direct towards a specific goal with innate driving forces.( Keser,
2009; 88).
Work motivation is a motive that is a person who works in an organization directed to do his/
her job. The occurance this motive is due to some factors. This factors are researched in the
studies regarding with psychology in literatür. In The results of researches, significant results
regarding with motivational means are observed. Herzberg said that, in his studies in 1959
and 1966, the intrinsic factors that the employee motive to work and hygine or external
factors that led to de-motivation and dissatisfaction in the absence of them.According to
theory, intrinsic factors are related to the nature of the work itself, achievement, recognition,
responsibility and advancement. The dissatisfaction factors are associated with the
individiual’ş relationship to the environment in which he/she does his/her work. The most
important of these is company policy and administration that promotes ineffectiveness within
the organization. The other factors are supervision, working conditions, interpersonal
relationship, salary.(Eren , 2010: 511-512, Landy and Conte, 2010: 370-371).
The work motivation is the important concept in the Industrial or organizational psychology.
For organizations to succeed, increasing the productivity of employees is very important.
Increasing the productivity of employees relates to employess’ quality of work life and
employees’ happiness in the workplace.
Quality of work life, to provide the bestworking conditionsfor employeesin a workplace.
Moreexpressly, all factors related to the workplace fromthe physicalcharacteristics ofthe
workplace to the politics ofthe organization'smanagement and all factorsrelated to the
workplaceand employeesto allow forloving his/her job, the jobcharacteristics
andintrinsicfactorsrelated
to
theprovision
ofbusiness-compliance
refers
tothe
individual.(Barutçugil, 2004; 394, 395,396).
As shown inthis conceptualexplanations, the employee's job motivationfactors that, in fact,
elementsthat make upthe quality ofwork life. Therefore,with therise ofthe working
individual’sworkmotivation, positive impact onquality of work lifewillincrease.
Intrinsicmotivationfactors inimproving thequality ofwork life,organizationalpoliciesto
increase theindividual'smotivation toworkby raisingthe quality of life, increasebusiness
efficiency, increase theperformance. As a result ofall these positivedevelopments,the
organizationwill showa good performanceas soon as possible and it will succeed.The presence
ofsuccessfulorganizationsin a countryand its economya positive impact onthe welfare ofthe
countrywill betaken on important roles.
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Motivationconcerns the conditions responsible for variations in intensity, persistence, quality,
and direction of ongoing behavior (Vinacke, 1962). The term “instinct” was gradually
replaced with terms such as “need”, “motive”, and “drive” (Viteless, 1953). Latham and his
colleagues (Latham&amp; Budworth, 2007; Latham&amp;Pinder, 2005) have reviewed the 100 year
history of motivation research in I-O psychology.Vroom (1964), proposed that VIE theory.
VIE consist of valence, instrumentality, expectancy. Vroom reasoned that psychological
objects in an environment also have attracting forces.
Between 1940 and 1960, the struggle for preeminence in motivation theory was between the
behaviorists and the need theorists. By 1960, the emergence of cognitive psychology resulted
in a radical shift in the battle for “motivational superiority”. New motivational theories
emerged that emphasized the thought and decision processes of the individual. Today’ş
theories of motivation are largely cognitive and emotional at their foundation. The differences
among them are more a matter of what people think about and how they think about choosing
courses of action, rather than any dispute regarding whether thought enters into motivation.
(Landy and Conte, 2010: 362).
Weiner (1991,1992) suggested that the best way to gain an understanding of the wide
variation in motivational theories, as well as of the evolution of motivational thinking, is
through the use of metaphors. A metaphor is intended to illuminate an obscure or difficult
concept by example. Weiner suggests that all motivational theories can be described by one of
two metaphors: the person as a machine, and the person as scientist. Person as machine:
Automatic response by individual, Responds to needs and drives, Responds to external stimuli
and reinforcement. Person as scientist: Voluntary response by individual: analyzes internal
and external information. Hypothesizes about the foundation for events and actions of others.
Develops goal and action plans.
Abraham Maslow (1943), suggested that the hierarchy of needs theory. Herzberg (1966),
revealed that two-factor theory. Locke (1980), motivation theory, behaviorism has addressed
a critical look at the strengths and shortcomings. Georgopoulos, Mahoney and Jones (1957),
have proposed the path-goal theory.
Adams (1965), proposed the ctheory of popular scientist. Festinger (1957), proposed the
theory of dissonance. Adams (1965), proposed by Festinger's ideas and improving the wellknown theory of "equality" has become a theory. Latham and Pinder (2005), examined, and
had proposed the theory of equality. Conner (2003), Bolino and Turnley (2008), studied the
theory of equality. Locke and Lactham (2002), researched goal-setting theory. Baldamu
(1951), Locke, Shaw, Saari, and Latham (1981), Ryan, (1970), goal-setting theory, Austin and
Vancouver (1996), goal-setting theory, Locke (1968), goal-setting theory, Locke and Latham
(1990), goal-setting theory, Locke and Latham (1996), goal-setting theory, Wiese and Freund
(2005), said that goal difficulty is a critical ingredient to both performance and satisfaction.
Mitchell, Thompson, George, Falvy, (2000), goal-setting theory, concept of commitment.
Locke and colleagues (1981), proposed that goal-setting theory, direction, effort, persistence,
strategy, feedback loop. Atkins, Wood, Rutgres, (2002), goal-setting theory, cognitive tasks.,
Youngcourt, Beaubien (2007) proposed that goal orientation prediction person’s achieve
280

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

work. Porath and Bateman (2006) proposed that specific self-regulatory tactics. Gollwitzer
(1990) supposed that focus on internal or external cues. Kuhl (1992) supposed that focus on
internal or external signs. Austin and Klein (1996) proposed that cognitive abilities roles.
Kanfer (1992) supposed that indivuduals need to depend on cognitive abilities (memory,
reasoning, comprehension).

2.METHOD
Questionnaire was administered to employees of the Bank. Data were analyzed using
SPSS 11 statistical package. Questionnaires were adapted by inspired from Erol Eren’s
motivational tools to encourage the scale of the survey, job satisfaction, job involvement and
an
Minnesota
job
satisfaction
Questionnaire.
All bank employees contained in Isparta city asked to be reached in the analysis, reached 140
people. 120 people were taken back. 102 form of these forms in the questionnaire that is
appropriate for analysis have been analyzed. Surveys have been done of face to face method
by the forms giving people exactly.
Analyses of demographic characteristics, frequency and percentage were analyzed.
The factor analysis has done for questions that measure work motivation and the quality .
Questions are grouped under four factors by factor analysis. By regression analysis Factor
groups between satisfaction with the quality of work life, the effects of these factors on
satisfaction with the quality of work life were measured. Difference tests were conducted for
all demographic characteristics to measure whether differences exist in terms of the quality of
work life satisfaction according to demographic characteristics And the results are described.

3.RESULTS AND DISCUSSION
Analysis were researched as shown below. Fistly, frequencies tables are observed. The
statistical analysis sources are used in the analyses. (Sipahi e.a., 2006, Albayrak, 2006).

3.1.Frequencies Tables
According the statement of gender, freguency column, 50 people of respondents are
male, and 52 people are female and to the percent column, 49 percent of respondents is male
and 51 percent is female.
Male/Female

Valid

281

Frequency

Percent

Valid
Percent

Cumulative
Percent

Male

50

49,0

49,0

49,0

Female

52

51,0

51,0

100,0

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Total

102

100,0

100,0

Likewise, according the statement of age, frequency and percent are observed.

Age

Valid

Frequency

Percent

Valid
Percent

Cumulative
Percent

20-25 age

18

17,6

17,6

17,6

2,00

40

39,2

39,2

56,9

3,00

28

27,5

27,5

84,3

4,00

10

9,8

9,8

94,1

5,00

6

5,9

5,9

100,0

Total

102

100,0

100,0

And the frequency and percent results are observed in the following frequency tables
such as Level Of Education, Job Title, Marital Statu, Salary, Working Time At The Bank,
Sectoral Working Time and Bank Status.

Level Of Education

Valid

Frequency

Percent

Valid
Percent

11

10,8

10,8

10,8

7

6,9

6,9

17,6

University (BA)

72

70,6

70,6

88,2

Master of Science

11

10,8

10,8

99,0

Doctorate

1

1,0

1,0

100,0

Total

102

100,0

100,0

High School
College
degree)

282

(associate

Cumulative
Percent

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Job Tıtle

Valid

Frequency

Percent

Valid
Percent

Cumulative
Percent

Booth Personnal

55

53,9

53,9

53,9

Assistant Director

17

16,7

16,7

70,6

Director

12

11,8

11,8

82,4

Chief

13

12,7

12,7

95,1

Deputy Director

3

2,9

2,9

98,0

Manager

2

2,0

2,0

100,0

Total

102

100,0

100,0

Marital Statu

Valid

Frequency

Percent

Valid
Percent

Cumulative
Percent

Married

62

60,8

60,8

60,8

Single

40

39,2

39,2

100,0

Total

102

100,0

100,0

Salary

Valid

283

Frequency

Percent

Valid
Percent

Cumulative
Percent

750-1000 TL

20

19,6

19,6

19,6

1001-1250 TL

21

20,6

20,6

40,2

1251-1500 TL

16

15,7

15,7

55,9

1501-1750 TL

15

14,7

14,7

70,6

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

1751-2000 TL

11

10,8

10,8

81,4

2001 TL and up 19

18,6

18,6

100,0

Total

100,0

100,0

102

Working Time At The Bank

Valid

Frequency

Percent

Valid
Percent

Cumulative
Percent

0-1

13

12,7

12,7

12,7

1-5

39

38,2

38,2

51,0

5-10

26

25,5

25,5

76,5

10-15

14

13,7

13,7

90,2

15-20

3

2,9

2,9

93,1

20 and up

7

6,9

6,9

100,0

Total

102

100,0

100,0

Frequency

Percent

Valid
Percent

Cumulative
Percent

0-1

10

9,8

9,8

9,8

1-5

42

41,2

41,2

51,0

5-10

23

22,5

22,5

73,5

10-15

16

15,7

15,7

89,2

15-20

4

3,9

3,9

93,1

20 and up

7

6,9

6,9

100,0

Total

102

100,0

100,0

Sectoral Working Time

Valid

Bank Status
Frequency

Percent
Valid

284

Cumulative

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Valid

Percent

Percent

Public

33

32,4

32,4

32,4

PrivateTurkish

53

52,0

52,0

84,3

PrivateForeign

16

15,7

15,7

100,0

Total

102

100,0

100,0

3.2.Factor Analysis
After reliability analysis, factor analysis was conducted. By the table checked, the
factor analysis consists of just a question was removed for it will not be healthy for the
analysis , factor analysis was again done. As a result, the load factor is still close to each other
for two separate factor group analysis of the questions in the order removed, the analysis was
repeated in the order. Four questions were removed. These four questions; incentives and
awards, granting a job he/she dislike, status of equipment and in-kind donations are affecting
the success of the workplace. Removing the effect level while the questions are considered by
the study. The latest results have emerged, such as factor analysis in the the following table.
Questions of recognition and appreciation and promoted close under the loads seen that the
two factors, however, that important questions for the purpose of the study were eligible for
the disconnection of. The factor analysis of the final table were seen that is significant and
analysis is continued
Rotated Component Matrix(a)
Component
1

2

3

4

Resposnsibility

,701

,392

-,018

,228

Participation in decision
making

,679

,244

,018

,308

,674

-,005

,315

,204

,673

,136

,435

,009

Work itself

Possibility of growth
and advancement

285

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Achievement
,662

-,083

,489

,130

,613

,506

,064

-,118

Appriciation and
recognition

,567

,415

,203

,212

Distrubition of tasks
between employee

,038

,869

,102

,156

,236

,773

,230

,050

,248

,696

,012

,342

,267

,197

,741

-,012

-,007

-,001

,732

,334

,250

,039

,686

,265

,152

,338

,579

,169

Possibility of holiday in
workplace

,103

,214

,121

,810

Job security

,126

,075

,354

,770

Promotion

The justice of
achievement,
appriciation, reward ın
organization

Salary

Interpersonal relations
superior

Healty benefits
Interpersonal relations
peers

Working conditions

286

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

,274

,143

,175

,653

Excessive working

According to this table he motivational factors are grouped under the four factors.
Looking at the quastions in the first factor, this factors are internal factors that individiual to
be succesful at work and providing motivate to work such as resposnsibility, participation in
decision making, work itself, possibility of growth and advancement, Achievement,
promotion, appriciation and recognition are seen.

The second factor group is related to task distribution between employee at the
workplace, wage levels in the workplace the justice of achievement, appriciation, reward ın
organization. This factor group brings together for the questions that affect perceptions of
justice is observed.

The third factor group is related to inter personal relations with superior or boss, inter
personel relations with peers, physical conditions at the workplace and health services
provided by the institution, indicate external factors with related to working conditions in the
workplace.

The last factor on the 4th group is related to possbility of holiday in the workplace,
job security and excessive working in the workplace, on personnel needed to feel comfortable,
job security guarantees, the right to allow use on a regular basis and the opportunity to work
within a normal period and the rest of the time will be important for personal life quality.
This group is concerned with employye rights. The sum of all factors that gives the quality of
work life.
FACTOR 1

FACTOR 2

Internal Factors

Working External Perception
Conditions Factors
Justice Factors

Resposnsibility

distrubition of tasks
between employee

participation in
decision making

the justice of
achievement,
appriciation, reward
ın organization

287

FACTOR 3

interpersonal
relations superior

FACTOR 4
Of Employee
Factors

possibility of holiday
in workplace

job security
healty benefits

Rights

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

work itself

Salary

interpersonal
relations peers
excessive working

possibility of growth
and advancement

working conditions

Achievement

Promotion

appriciation and
recognition

3.3.Regression Analysis

After the factor analysis, the regression analysis was done between the factor groups
that occured in the factor analysis and the quality of work life scale to observe whether a
significant relationship between motivational instruments and the work life quality. In the
analaysis, The factor groups are independent variables and the quality of work life satisfaciton
is dependent variable.
Model Summary(b)

Model

R

1

,781(a)

R Square
,611

Adjusted
R Square

Std. Error of
the Estimate

,594

,68856

ANOVA(b)
Sum of
Squares

Model
1
288

Regression

72,099

Df

Mean Square
4

18,025

F
38,017

Sig.
,000(a)

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Residual
Total

45,989

97

118,088

101

,474

Coefficients(a)

Unstandardized
Coefficients
Model
1

B
(Constant)

Std. Error

3,618

,068

REGR factor
score 1 for
analysis 1

,581

,069

REGR factor
score 2 for
analysis 1

,438

REGR factor
score 3 for
analysis 1
REGR factor
score 4 for
analysis 1

Standardized
Coefficients

t

Sig.

Beta

B

Std. Error

53,062

,000

,537

8,480

,000

,069

,405

6,391

,000

,154

,069

,143

2,253

,027

,401

,069

,371

5,852

,000

Because of Significant values are less than 0, 05 that the hypothesis test is meaningful.
Regression analysis shows that the relationship between the variables. Factors that increase
the motivation affects job satisfaction and improve the quality of work life.
Due to the results, there is a significant relationship between motivational tools and
quality of work life. When The work motivation is higher by using motivational tools, the
employees’ satisfaction related to the quality of work life increases. On the contrary, in the
absence of motivational instruments, the satisfaction related to the quality of work life
decreases.
So, the organizations should use motivational instruments, try to raise work motivation
of employees and thus improve quality of work life of employees.

289

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

3.4.Nonparametric Difference Tests
Firstly, normality was researched for work life quality variable. And then, the
difference tests were done according to the demographic varables.
Tests of Normality
Kolmogorov-Smirnov(a)
Statistic
The Quality of Work-life
Satisfaction

df

,315

Shapiro-Wilk

Sig.
102

,000

Statistic

Df

,832

Sig.
102

,000

a Lilliefors Significance Correction

Ho : The Quality of Work-life Satisfaction is suitable for Normality
Because of the p value is less than 0,05, Ho P&lt; 0.05 olduğundan Ho hypothesis is
rejected, is not suitable for normality. Therefore, demographic variables related to The
Quality of Work-life Satisfaction was analyzed with nonparametric tests.
In the Following analyses, The Ho Hypothesis is that the medians of demographic
variables are equal. And The all Ho Hypotheses are formed in the same way for all of
demographic variables.

Ranks
Male/
Female
Quality of Work-Life
Satisfaction

N

Mean Rank Sum of Ranks

50

51,10

2555,00

Female

52

51,88

2698,00

Total

102

Male

Test Statistics(a)

Quality of
Work-Life
Satisfaction
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Mann-Whitney U

1280,000

Wilcoxon W

2555,000

Z

-,145

Asymp. Sig. (2tailed)

,885

Ho: median are equal.
H1: The median is not equal.
Ho hypothesis is accepted. P value 0.885&gt; 0.05 is considered that the median are equal.
Ranks
Egitim
Quality of Work-Life
Satisfaction

High School

39,55

7

64,43

University (BA)

72

52,94

Master of Science

11

45,09

1

59,50

Doctorate
Total

Test Statistics(a,b)

Quality of
Work Life
Satisfaction

Df
Asymp.
Sig.

291

Mean Rank
11

College (associate
degree)

Chi-Square

N

4,539
4
,338

102

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Chi-square statistic and p value are examined, because of p-value is greater than value of 0.05
the Ho hypothesis is accepted. The quality of work life satisfaction do not differ by educational
attainment.
Ranks
Salary
Quality of Work Life
Satisfaction

N

Mean Rank

750-1000 TL

20

51,33

1001-1250 TL

21

44,12

1251-1500 TL

16

47,44

1501-1750 TL

15

47,53

1751-2000 TL

11

62,64

2001 TL and up

19

59,95

Total

102

Test Statistics(a,b)
Quality of
Work Life
Satisfaction
Chi-Square
Df

5,815
5

Asymp.
Sig.

,325

Ho hypothesis is accepted because of p-value is greater than value of 0.05 and the
quality of work life do not differ by salary is considered.
Ranks
Job Title
Quality of Work Life
Satisfaction

292

N

Mean Rank

Booth Personnel

55

47,77

Assistant Director

17

47,68

Director

12

57,33

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Chief

13

53,19

Deputy Director

3

82,50

Manager

2

94,00

Total

102

Test Statistics(a,b)
Quality of
Work Life
Satisfaction
Chi-Square
Df
Asymp.
Sig.

10,597
5
,060

Ho Hypothesis is accepted, the p value is greater than 0.05. However, it must be said,
0.06 is a value close to the value of 0.05. Mean values of rank titles, deputy director and
director, the positions occupied by the lower difference which showed. Deputy director and
director of the quality of work life satisfaction varies for titles.
Ranks

Bank Status
Quality of Work Life
Satisfaction

Public

Mean Rank

33

55,67

Private-Turkish

53

51,06

Private-Foreign

15

40,53

Total

293

N

101

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Test Statistics(a,b)

Quality of
Work Life
Satisfaction
Chi-Square

3,197

Df

2

Asymp.
Sig.

,202

Ho hypothesis is accepted because of p-value is greater than value of 0.05 and the
quality of work life do not differ by bank status is considered.
Ranks
Working Time
At The bank
Quality of Work Life
Satisfaction

13

52,58

1-5

39

43,64

5-10

26

50,06

10-15

14

59,50

15-20

3

71,00

20 and up

7

74,29

Test Statistics(a,b)
Quality of
Work Life
Satisfaction

Df

294

Mean Rank

0-1

Total

Chi-Square

N

10,857
5

102

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Asymp.
Sig.

,054

Ho hypothesis is accepted because of p-value is greater than value of 0.05 and the
quality of work life do not differ by working time at the bank is considered. However,
observed differences over 15 years.
Ranks
Marital
Status
Quality of Work Life
Satisfaction

N

Mean Rank Sum of Ranks

62

53,54

3319,50

Single

40

48,34

1933,50

Total

102

Married

Test Statistics(a)
Quality of
Work Life
Satisfaction
Mann-Whitney U

1113,500

Wilcoxon W

1933,500

Z

-,936

Asymp. Sig. (2tailed)

,349

Ho hypothesis is accepted because of p-value is greater than value of 0.05 and the
quality of work life do not differ by marital status is considered.
Ranks
Age
Quality of Work Life
Satisfaction

295

N

Mean Rank

20-25 age

18

56,89

2,00

40

42,64

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

3,00

28

52,43

4,00

10

59,50

5,00

6

76,75

Total

102

Test Statistics(a,b)

Quality of
Work Life
Satisfaction
Chi-Square
Df
Asymp.
Sig.

10,864
4
,028

Hypothesis Ho is rejected, because of the p value is less than the value of 0.05, the
quality of work life varies according to age element.
As a result of , there is the significant relationship between motivation and the quality
of work life is observed in this study. In terms of Suistainable development, in countries, the
success and effectiveness of instuitaions is quite important. One of the most important
elements of providing success and effectiveness of institutions that have the human resources
is working effectively and efficiently. In this respect, “motivation” is one of the most
important factors in raising the quality of work life in the “institutions, employee productivity,
the contribution of the institution, and the sense of belonging in the institution. Motivation
plays an important role in business life. The work motivation increases the concern of the
employee's job, and as a result of raising the quality of work life is an important factor that
increase organizational efficiency. The higher motivation increases the contribution of the
institution by raising the productivity of the employee. High rate of success of the
organization by increasing employee motivation provided by the institution will contribute to
the country's economy. This aspects, in order to take advantage of the present institutions
active human sources, making efforts to enhance the motivation of employees in achieving
organizational effectiveness and efficiency and provide the employees' job satisfaction.
Happy and productive working lifes of employees in having a sense of belonging in the
institution can be used effectively in improving the motivation factor is the result. The higher
motivation increases the productivity of employee, institutional effectiveness and quality of
working life. Motivating factors should be applied by institutions.
296

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

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                <text>The effects of work motivation in quality of work life and  A study on banking sector</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="96">
            <name>Author</name>
            <description>Author</description>
            <elementTextContainer>
              <elementText elementTextId="17848">
                <text>Esra, Zeynel</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="94">
            <name>Abstract</name>
            <description>A summary of the resource.</description>
            <elementTextContainer>
              <elementText elementTextId="17849">
                <text>In terms of Suistainable development, in countries, the success and effectiveness of  instuitaions is quite important. One of the most important elements of providing success and  effectiveness of institutions that have the human resources is working effectively and  efficiently. In this respect, “motivation” is one of the most important factors in raising the  quality of work life in the institutions, employee productivity, the contribution of the  institution, and the sense of belonging in the institution. Motivation plays an important role in  business life. Motivation by definition means that the individual actuating motive. In the  business life, motivation, increase the concern of the employee's job, and as a result of raising  the quality of work life is an important factor that increase business efficiency. The higher  motivation increases the contribution of the institution by raising the productivity of the  employee. High rate of success of the organization by increasing employee motivation  provided by the institution will contribute to the country's economy. This aspects, in order to  take advantage of the present institutions active human sources,making efforts to enhance the  motivation of employees in achieving organizational effectiveness and efficiency and provide  the employees' job satisfaction. Happy and productive working lifes of employees in having a  sense of belonging in the institution can be used effectively in improving the motivation  factor is the result.This study measured the effect of bank employees, the care component of  motivation in raising the quality of work life survey was applied on the bank employees. In  this study, a survey was applied on bank employee in terms of assessment of the effect of  motivation in the raising the quality of work life of bank employee. Importance of motivation  arises in this sector. The higher motivation increases the productivity of employee,institutional effectiveness and quality of working life. Motivating factors should be applied by  institutions.</text>
              </elementText>
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          <element elementId="40">
            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="17850">
                <text>2012-05-31</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="97">
            <name>Keywords</name>
            <description>Keywords.</description>
            <elementTextContainer>
              <elementText elementTextId="17851">
                <text>Conference or Workshop Item
PeerReviewed</text>
              </elementText>
            </elementTextContainer>
          </element>
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                    <text>Carrillo_Morales G., Davila-Jimenez M.M., Elizalde-Gonzalez M.P., Pelaez-Cid A.A,
Removal of Metal Ions From Aqueous Solution by Adsorption on the Natural Adsorbent
CACMM2, Jaurnal of Chromatography A, 938, 237-242, 2001.
Townshend, A; Encylopedia of Analitical science, Academic Pres, Londan, 1995.
Water Pollution Control Regulation Official Gazette: 31 December 2004, Issue: 25687.
Özdemir C. et al., ―Examination of purification of leather industry wastewaters with the use of
the marl soils of Basyayla(Karaman) region‖, D.E.U Science and Engineering Journal, Issue:
3 5.57-64, October 2004.
Toprak R.Girgin I., ―Removal of chromium from leather industry wastewaters with activated
clinoptilolite‖, Turk J Engin Enviran Sci 24(2000), 343-351 TUBITAK.

Water Resources Conservation for Sustainable Agricultural Development
F. Ozge Uysal, Onder Uysal
Faculty of Agriculture, SuleymanDemirel University, Isparta, Turkey
E-mail: onderuysal@sdu.edu.tr
1.INTRODUCTION
1.1.Water resources consumption
Increase of population and insufficient water resources has led to environmental pollution has
reached substantial levels with use of synthetic and chemical inputs as a result of the increase
in production. Decreasing available water resources brings on a serious water shortage
problem. Allliving things are negative affected by theenvironmentalpollution. The loss of
speciesin nature, instead of use of biological control use chemical control, thehormonesto
increasefood production have a verynegativeforecology. The main reason for environmental
pollution and degradation of the natural balance in ecosystem, consumption increased rapidly
due to increasing population and increased use of fossil proliferation of products.
1.2.Water resources conservation
One
of
themostimportantaim
of
sustainableagricultureandruraldevelopment
is
toprotectandconservethecapacity of thenaturalresourcebasetocontinuetoprovideproduction,
environmentalandculturalservices.

333

�Turkey Land and Water Resources
LAND RESOURCES
Mha ( millionhectares)
Arable
Irrigable
RainfedAgriculture
EconomicallyIrrigable
PresentlyIrrigated

Land :
Land :
:
:
: 4.90

28.05
25.75
17.25
8.50

Land Resources

Turkey‘s
total
landarea is 78 Mha. Almostonethird of this, 28 Mha, can be classified as cultivableland.
Recentstudiesindicatethat
an
area
of
about8.5million
ha
is
economicallyirrigableundertheavailabletechnology. Untilnow, an area of about2.8million ha
has beenequippedwithirrigationinfrastructuresby DSİ.
WaterResources

MeanPrecipitation

643 mm/m2

Turkey‘sSurfaceArea

780,000 km 2

AnnualWaterResourcesPotentialBm ³ (billion m ³ )
A Precipitation Volume

501

B Evaporation

274

C LeakageintoGroundwater

69

D Springs FeedingSurfaceWater

28

E SurfaceWaterfromNeighboringCountries

7

F=A-B-C+D+E
F Total SurfaceRunoff (gross)

193

G ExploitableSurfaceRunoff

98

H GroundwaterSafeYield

14

I=G+H
I Total Potential (net)

112

The total watervolume in theworldamountsto1.4billion km3, 97.5% of which is salinewater in
theoceansandseas, 2.5% of which is freshwater in theriversandlakes. Duetofactthat 90% of
334

�freshwaterexists
in
the
South
Poleand
humanbeingshaveverylimitedreadilyexploitablefreshwaterresources.

North

Pole,

Annualmeanprecipitation in Turkey is 643 mm, whichcorrespondsto 501 Bm3 (billion m3) of
annualwatervolume
in
thecountry.
A
volume
of
274
Bm3waterevaporatesfromwaterbodiesandsoilstoatmosphere. 69 Bm 3 of volume of
waterleaksintogroundwater,
whereas
28
Bm3
is
retrievedbyspringsfromgroundwatercontributingtosurfacewater. Also, thereare 7 billion
m3volume
of
watercomingfromneighboringcountries.
Thus,
total
annualsurfacerunoffamountsto a volume of 193 Bm3 of water.
Turkey is not a richcountry in terms of existingwaterpotential. Turkey is a
waterstresscountryaccordingtoannualvolume
of
wateravailablepercapita.
Theannualexploitableamount of water has recentlybeenapproximately 1,500 m3percapita.
Development of Irrigation, Hydropower, andWaterSupplySectors in Turkey
IN
ULTIMATE
OPERATION GOALS
AS OF 2005
DEVELOPMENT
IRRIGATION

OF

BY 2030

4.9 million ha 8.5 million ha

DEVELOPMENT OF
45.3
HYDROELECTRIC
billionkWh
ENERGY

127.3
billionkWh

DEVELOPMENT OF
WATER SUPPLY FOR 10.5 billion m
38.5 billion m 3
DOMESTIC
AND 3
INDUSTRIAL USE

EACH
SECTOR‘S
DEVELOPMENT
RATES
58%

36%

27%

1.3.Orientationtoecotourism
Peopleturned toecotourismareas because of environmental pollution, increase in population,
global warming. Ecotourism increased because of peoplemove away fromcity life,
increasedconsumption of products ofagriculturalorigin and increase of environmental
pollution.Because of reduction of water resources, production activities also reduced.
Difficulties which are encountered in production activities because of water resources are
deficit in our country. Decreasing available water resources brings a serious water shortage
problem. In order to deal with this problem, the studies for the efficient use of irrigation water
by providing water saving gain importance. Irrigationtechniques have been developed for
efficient use of water resources. Withthe effective useof water resources, boththewater
resourceswill be protected and high yieldwill be obtained per unit area. So, peoplecan cometo
workforecotourismactivities. Thus, environment, social life, cultural environment and public
health develop positive because of ecotourism. Everykind of tourismmade in virginnature is
withinthescope of ecotourism. Theprinciple of ―sustainability of naturalenvironment‖, which
is one of thetwoimportantcriteria of ecotourism in thesenaturetrips, has to be
obeyedclosely.Thesecondimportantcriterion
of
ecotourismis
―thesustainability
of
335

�localculturesandthatthepeopleshouldbenefitfromthistourismactivity‖. When eco-tourists make
tourism activities, they can do agricultural activities. Such water resources, environmental
pollution and global warming factors can provide with conservation ofwater quality. This also
provides that effectivefertilizerin agricultural areas, efficient use ofpesticides and efficient
useof water resources.
2.RESULT
Therichgeographyandnaturalpotential of ourcountry is a bigchanceforthetypes of
naturetourisms.
However,
if
it
is
behavedunconsciously,
theruin
of
environmentalvalueswillrapidly be inevitable.Sustainableagriculturemay be defined as
consisting of environmentally-friendlymethodsof farmingthatallowtheproduction of
cropsorlivestockwithoutdamagetohumanornaturalsystems.Recently,
orientationtoecotourismstudyingshouldincreaseandthus,
agriculturalenviromentalandglobalwarmingproblemsshould
be
solved.Theuse of
agriculturalproducts ratherthan theuse
of
syntheticproductsshouldbe
increase.
Not
onlyenvironmentalandtourismpurposesanndeclining waterresources, increase of population
and
in ordertomeet growing consumer needsshould
be
provided in thedevelopment
of ecotourism.
REFERENCES
http://www.ekoturizmdernegi.org
http://www.dsi.gov.tr

Sustainable Development- Environmental Ethics Relationship Within Eu
Environmental Policies
Özcan Sezer, Şenay Işin
1.INTRODUCTION
Environmental problems and approaches to environment have an important place within EU
policies. There are major impacts of economic development on the inclusion of environmental
issues to the Union‘s area of interest, which aims to integrate European Countries through
economic, political and cultural areas and which foresees the free flow of capital, goods,
services, labor. It is crucial to form environmental values and provide the member countries to
internalize them in order to sustain development without giving harm to environment. In this
context, environmental ethics reveals a conflict from the perspective of sustainable
development. There emerges an area of conflict between attaining economic, social and
cultural development on the one hand and taking into consideration of environmental values
and environmental ethics during this process on the other hand.
The desire to improve the living conditions at the member countries through providing
economic, social and cultural development and to upgrade quality of life to a common level
336

�</text>
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                <text>F. Ozge Uysal, Ozge Uysal</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="94">
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            <description>A summary of the resource.</description>
            <elementTextContainer>
              <elementText elementTextId="17856">
                <text>Increase of population and insufficient water resources has led to environmental pollution has  reached substantial levels with use of synthetic and chemical inputs as a result of the increase  in production. Decreasing available water resources brings on a serious water shortage  problem. Allliving things are negative affected by theenvironmentalpollution. The loss of  speciesin nature, instead of use of biological control use chemical control, thehormonesto  increasefood production have a verynegativeforecology. The main reason for environmental  pollution and degradation of the natural balance in ecosystem, consumption increased rapidly  due to increasing population and increased use of fossil proliferation of products.</text>
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            </elementTextContainer>
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                    <text>3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Concepts and Practice. Greenleaf Publishing Limited, Sheffield.
Seçme, N.Y., Bayrakdaroğlu, A. and Kahraman, C. (2009) Fuzzy performance evaluation in
Turkish Banking Sector using Analytic Hierarchy Process and TOPSIS, Expert
Systems with Applications, 36(9), 11699-11709.
Sheu, J.B., Chou, Y.H., Hu, C.C. (2005) An integrated logistics operational model for greensupply chain management, Transportation Research Part E, 41(4), 287-313.
Srivastava, S.K. (2007) Green supply-chain management: A state-of-the-art literature review,
International Journal of Management Reviews, 9 (1), 53-80.
Vachon, S. and Klassen, R.D. (2008) Environmental management and manufacturing
performance: the role of collaboration in the supply chain, International Journal of
Production Economics, 111 (2), 299-315.
Van Hoek, R.I. (2000) From reversed logistics to green supply chains, Logistics Solutions, 2,
28–33.
Zhu, Q. and Sarkis, J. (2004) Relationships between operational practices and performance
among early adopters of green supply chain management practices in Chinese
manufacturing enterprises, Journal of Operations Management, 22, 265–289.

Selection of Sustainable Warehouse Location in Supply Chain Using the Grey
Approach

Fahriye Uysal1,Ömür Tosun2
1Department of International Trade and Logistics, Ayse Sak School of Applied Sciences,
Akdeniz University, Antalya, Turkey
2Department of Business Administration, Faculty of Economic and Administrative Sciences,
Akdeniz University, Antalya, Turkey
E-mails: fahriyeuysal@akdeniz.edu.tr,omurtosun@akdeniz.edu.tr

Abstract
Supply chain sustainability has recently gained an increasing attention in the supply chain
context both from the practitioners’ perspective and as a research area. There have been many
incentives for more sustainable warehousing in supply chains. Sustainable Warehousing
includes activities such as, for example, terminal and warehouse location, proper storing and
383

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

disposing of hazardous materials, donation of excess or obsolete inventory to local
communities, and training to safely operate forklifts. A sustainable warehousing company
would not only have to consider the economic factors, such as rent and operations costs, but
also balance the social and environmental effects that occur within the warehouse compound
as well as its surrounding vicinity. Sustainable warehouse location selection decision has a
crucial role in the supply chain management process. In this study cost, labor, transportation,
environment and geographical location are taken as decision criteria. Grey approach is used
under the sustainability basis for the warehouse location selection problem. This method is
appropriate for solving the group decision-making problem in an uncertain and inconsistent
environment. Warehouse location alternatives for the supply chain of the medical companies
are evaluated in this study.

Keywords: Sustainable supply chain, sustainable warehousing, grey approach

1. INTRODUCTION

The term sustainability, which increasingly refers to an integration of social, environmental,
and economic responsibilities, has begun to appear in the literature of business disciplines
such as management and operations. In addition, companies are beginning to rapidly adopt
the term sustainability (Carter and Rogers, 2008). Sustainability today demands that supply
chains must be explicitly extended to include by-products of the supply chain (Linton et al.,
2007).

The need of seriously exploring the concept of sustainable supply chains within a
collaborative perspective is seen as a goal to improve companies’ revenue growth and
costumer’s recognition (Kleindorfer, et al., 2005). A focus on supply chains is therefore a
step towards the adoption of a growth on sustainability.

The location of a warehouse is generally one of the most important and strategic decision in
the optimization of supply chain (Frazelle, 2002). It is a long-term decision and can be
influenced by different quantitative and qualitative criteria, but not all of the criteria have the
same effect on the decision process. In this study costs, labor, transportation, environment
and geographical location criteria are used. Due to the prejudice of the decision makers,
conventional approaches can be less effective in dealing with a proper assessment.
Therefore, a multi-criteria based decision making procedure is used.

This paper describes the Grey approach and its application for evaluating warehouse
locations in supply chain based on sustainability criteria. This method is appropriate for
384

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

solving the group decision-making problem in an uncertain and inconsistent environment.
The work is structured in the following manner. In Section 2, a literature review of the
sustainable supply chain is given. In Section 3 definition of sustainable warehousing is given.
In Section 4, the grey approach is defined. An evaluation of warehouse location selection, the
proposed methodology and results are fully shown in Section 5. Finally, conclusions and
considerations are reported in Section 6.

2. Sustainable Supply Chain

A focus on supply chains is a step towards the broader adoption and development of
sustainability, since the supply chain considers the product from initial processing of raw
materials to delivery to the customer (Linton et al, 2007). Extending the supply chain to
include issues such as remanufacturing, recycling and refurbishing adds an additional level of
complexity to existing supply chain design in addition to a new set of potential strategic and
operational issues, which in turn can increase costs, at least in the short term (Corbett and
Klassen, 2006). Sustainable supply chains have not yet been clearly defined. A popular
definition states that sustainable supply chains require coordination of the social,
environmental and economic dimensions. Linton et al. (2007) transfer the concept of
sustainability to supply chains and state that: a sustainable supply chain is a supply chain
integrating issues and flows that extend beyond the core of supply chain management such as
product design, manufacturing by-products, product management during use, product life
extension, product end-of-life and recovery processes at end-of-life.

Companies that implement ecological and social factors relating to the supply chain gain
financial benefits through reduced costs and operational efficiencies, also sustainable supply
chain practices can be a source of differentiation and competitive advantage.

3. Sustainable Warehousing

One of the key components within the sustainable supply chain is sustainable warehousing.
Sustainable warehousing includes activities such as terminal and warehouse location, proper
storing and disposing of hazardous materials, donation of excess or obsolete inventory to
local communities, and training to safely operate forklifts (Carter and Jennings, 2000).

385

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

In real life, most warehousing and transportation companies have little regard for the
environmental impacts of their actions and do not understand the social consequences of their
business activities. A sustainable warehousing company would not only have to consider the
economic factors like rent and operations costs, but also balance with the social and
environmental effects that occur within the warehouse compound as well as its surrounding
vicinity (Tan et al, 2010). Among supply chain studies, many papers on warehouse/facility
location problem have been published (Vlachopoulou et al. 2001; Sharma and Berry, 2007;
Sharma and Sharma, 2000). But, in most of them, environmental issues are not studied due to
the policy of these companies.

Different warehouse location selection studies are analyzed to select the suitable criteria, and
also environment criterion is added in view of the sustainability. These criteria are:
Cost: Costs are one of the factors highly affected by the facility location. Cost criterion is
evaluated by taking into account of labor costs, transportation costs, handling costs and land
cost.
Labor: This criterion defines the state of qualified labor at a location and the degree of the
availability of such labor.
Transportation: Ease of transportation, traffic density and distance from the alternative
warehouses to main warehouse are considered by the decision makers.
Environment: Carbon minimization and other environmental policies are considered for the
decision process.
Geographical location: This criterion defines the land availability that changes according to
the structure of the alternative regions.

4. The Grey Approach

In this section, the Grey approach will be described. In this method, there are m possible
warehouse alternatives given as
.
is the set of n
independent criteria.

is the vector of criteria weights. In this study,

criteria weights and ratings of the warehouses are taken as linguistic variables. These values
are given in Table 1 and Table 2. In Table 1, linguistic criteria weights whereas in Table 2
criteria rating values
in Grey numbers are given. A Grey number is shown as
.
The detailed procedure is given below (Li et al., 2007; Baskaran et al., 2012):
Step 1: Criteria weight identification
A group of decision makers (DM) identifies the criteria weights. If there is K number of
decision maker, then the criteria weight is calculated as
386

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

where

is the criteria weight of the Kth DM.

Step 2: Criteria rating value in linguistic variables
Criteria rating values in linguistic variables are calculated using

where

is the criteria rating value of the Kth DM.

Table 1: Scale of attribute weights
Scale
Very low (VL)
Low (VL)
Medium low (ML)
Medium (M)
Medium high (MH)
High (H)
Very high (VH)

W
[0.0, 0.1]
[0.1, 0.3]
[0.3, 0.4]
[0.4, 0.5]
[0.5, 0.6]
[0.6, 0.9]
[0.9, 1.0]

Table 2: Scale of attribute ratings
Scale
Very poor (VP)
Poor (P)
Medium poor (MP)
Fair (F)
Medium good (MG)
Good (G)
Very good (VG)

W
[0, 1]
[1, 3]
[3, 4]
[4, 5]
[5, 6]
[6, 9]
[9, 10]

Step 3: Establish the Grey decision matrix
are linguistic variables based on the grey number. Grey decision matrix is:

Step 4: Normalize the Grey decision matrix

In the normalization process, the ranges of the Grey numbers are limited to [0, 1].
Normalization depends on either minimization (cost) or maximization (benefit) of the criteria.
Maximization criteria
is given as

Minimization criteria

is given as below

Step 5: Establish the weighted normalized Grey decision matrix

The weighted normalized Grey decision matrix is the product of normalized Grey decision
matrix and criteria weights (
.
387

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Step 6: Set ideal warehouse alternative as referential warehouse alternative

From m possible warehouse alternative set (S = {S1, S2, …,Sm}) the ideal referential
warehouse alternative
by using

Step 7: Calculate the Grey possibilities
Compare warehouse alternatives set S = {S1, S2, …,Sm} with ideal referential warehouse alternative
;

Step 8: Prioritize warehouses

Rank the order of warehouse alternatives based on the comparison given in Eq. (7). If S i
value is smaller, the ranking order of Si is better. Otherwise, the ranking order is worse.

5. Application
In this study, the Grey approach is used for the warehouse location selection problem of a
pharmaceutical company in Turkey. The company has six different warehouse location
alternatives (S); each of them is evaluated by four decision makers (DM) according to the
criteria of cost (C1), labor (C2), transportation (C3), environment (C4) and geographic location
(C5). The calculation procedure is given below:

Step 1
By using Eq. (1), criteria weights are calculated.
Step 2
Criteria ratings values for six warehouse alternatives are calculated by using Eq. (2).
Step 3
Using Eq. (3), establish the grey decision matrix.
Step 4
Establish the grey normalized decision table.
Step 5
Establish the grey weighted normalized decision table.
Step 6
Make the ideal warehouse
388

a referential alternative, using Eq. (6).

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

= {[0.333, 0.950], [0.353, 0.650], [0.482, 0.925], [0.416, 0.725], [0.513, 0.825]}
Step 7
Calculate the grey possibility degrees, using Eq. (7). Grey possibility degree results are given
below:
= 0.862

= 0.729

= 0.686

= 0.963

= 0.716

= 0.735

Step 8
Rank the order of six warehouses, according to the results given in Step 7.
S5 &gt; S4 &gt; S3 &gt; S6 &gt; S1 &gt; S2
From the results, it’s seen that S5 is the most suitable location alternative for the company.

6. CONCLUSION
A warehouse location selection is a multi-criteria decision-making problem including both
quantitative and qualitative criteria. In this paper, a multi-criteria decision making approach
for sustainable warehouse location selection is given. The main criteria for the selection of
sustainable warehouse location were identified. After the main criteria were determined, the
hierarchy of the sustainable warehouse location selection was structured. The proposed
method is applied a pharmaceutical company, and decision makers from the company are
asked to evaluate alternatives and criteria. By using Grey approach, alternative 5 can be
selected by the company.

REFERENCES
Baskaran V., Nachiappan S. and Rahman S. (2012) Indian textile suppliers' sustainability
evaluation using the grey approach, International Journal of Production Economics,
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Carter, C.R. and Jennings, M.M. (2002) Logistics social responsibility: An integrative
framework, Journal of Business Logistics, 23(1), 145–180.
Carter, C.R. and Rogers, D.S. (2008) A framework of sustainable supply chain management:
moving toward new theory, International Journal of Physical Distribution &amp; Logistics
Management 38(5), 360-387.
Corbett, C.J. and Klassen, R.D. (2006) Extending the horizons: environmental excellence as
key to improving operations, Manufacturing and Service Operations Management, 8
(1), 5–22.
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Frazelle E. (2002) Supply Chain Strategy: The Logistics of Supply Chain Management,
McGraw-Hill, New York.
Kleindorfer, P.R., Singhal, K. and Van Wassenhove, L.N. (2005) Sustainable operations
management, Production and Operations Management, 14(4), 482–492.
Li, G., Yamaguchi D. and Nagai M. (2007) A grey-based decision-making approach to the
supplier selection problem, Mathematical and Computer Modeling, 46, 573-571.
Linton, J.D., Klassen, R. and Jayaraman, V. (2007) Sustainable supply chains: an
introduction, Journal of Operations Management, 25 (6), 1075-1082.
Sharma, R.R.K., and Sharma, K. D. (2000) A new dual based procedure for the transportation
problem, European Journal of Operational Research, 122 (3), 611-624.
Sharma, R.R.K., and Berry, V. (2007) Developing new formulations and relaxations of single
stage capacitated warehouse location problem: Empirical investigation for assessing
relative strengths and computational effort, European Journal of Operational Research, 177
(2), 803-812.
Tan, K.S., Ahmed, M.D. and Sundaram, D. (2010) Sustainable enterprise modeling and
simulation in a warehousing context, Business Process Management Journal, 16 (5), 871-886.
Vlachopoulou, M., Silleos, G., and Manthou, V. (2001) Geographic information systems in
warehouse site selection decisions, International Journal of Production Economics, 71 (1),
205-212.

The Role Of Internal Auditing In Sustainable Development And Corporate Social
Reporting

Serpil Senal, Gaye Atilla, Burcu Aslantaş Ateş
Süleyman Demirel University, Isparta, Turkey
E-mails:serduz@mynet.com, burcuatesaslantas@msn.com, gaisan@hotmail.com

Abstract
Sustainability means a lot more than just the economic, environmental, and social challenges
an organization faces in its everyday and future operations: it means the ethics in these
operations, touching on all the lives of those in the organization, its stakeholders, and the
planet. The objectives of all sustainable development programs must be measured, and the
390

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                <text>Supply chain sustainability has recently gained an increasing attention in the supply chain  context both from the practitioners’ perspective and as a research area. There have been many  incentives for more sustainable warehousing in supply chains. Sustainable Warehousing  includes activities such as, for example, terminal and warehouse location, proper storing and disposing of hazardous materials, donation of excess or obsolete inventory to local  communities, and training to safely operate forklifts. A sustainable warehousing company  would not only have to consider the economic factors, such as rent and operations costs, but  also balance the social and environmental effects that occur within the warehouse compound  as well as its surrounding vicinity. Sustainable warehouse location selection decision has a  crucial role in the supply chain management process. In this study cost, labor, transportation,  environment and geographical location are taken as decision criteria. Grey approach is used  under the sustainability basis for the warehouse location selection problem. This method is  appropriate for solving the group decision-making problem in an uncertain and inconsistent  environment. Warehouse location alternatives for the supply chain of the medical companies  are evaluated in this study.  Keywords: Sustainable supply chain, sustainable warehousing, grey approach</text>
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                    <text>3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Stock exchange influence in promotion of capital market in Bosnia and Herzegovina:
SASE case
Fahrudin Malanovic
International University of Sarajevo, Faculty of Management and Business Administration
71000, Sarajevo, Bosnia and Herzegovina
E-mails: malanovic_88@hotmail.com
Abstract
Economic efficiency and well being of one country is somehow connected with the efficiency
and effectiveness of stock exchange, so the main purpose of this study is to show and explain
workings of capital market in Bosnia and Herzegovina through activities of Sarajevo Stock
Exchange. The reason for this analysis is fact that the stock exchange performs important, if
not key, role in one nation’s economy since it provides investment opportunities by offering
buyers and seller’s places for trading securities, such as stocks and bonds. Such investment
opportunities are ways for corporations, governments, and business to obtain funds necessary
for expanding business or financing publicly good project.
Another reason for this analysis is the lack of awareness about importance of stock exchange
even if performance of stock exchanges affects everybody starting from individuals to large
corporations to governments.
Taking into consideration the fact that the stock exchange is an organized place for trading
goods, securities, financial derivatives…etc, whose daily volume is measured in billions of
dollars, it can be easily concluded that importance of it is huge.
This work is mentioning direct and indirect effects of stock exchange activity on an economy,
proving great impact on daily activity, not only of business, but of people as well.
This study also indicates that highly developed nations are closely related to the high level of
activity of stock exchanges and see stock exchange, as a component of capital market, a key
factor to success. Simply, stock exchange is as important as other public goods.
Less developed nations are not necessarily related to the poor performance of stock
exchanges; however, it could also be a main reason for those nations to be considered as a less
developed.
All in all, nothing can change the fact that stock exchange creates our destiny regarding our
future job orientation, since companies and corporations find stock exchange one of the
easiest ways to obtain money to finance their activities. Also, nothing can change the fact that
the whole global economy, including economy of Bosnia and Herzegovina is dependent on
those biggest stock exchanges around the world where the most important one is the New
York Stock Exchange.
This work is divided into three chapters. Section 1 deal with stock exchange and stock market
in general. When it comes to Section 2, it deals with stock exchanges in Bosnia and
Herzegovina, while Section 3 is actually conclusion part of this work.

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Keywords: stock exchange, capital market, security, stock, bond
1.INTRODUCTION
1.1 Historical review – stock market term
Stock exchanges have been existing for hundreds of years. Some of them are dating back to
year of 1100 such as stock exchange in Paris, stock exchange in Amsterdam...etc. So, as we
can see, the origin of stock exchanges is actually Western Europe. In the years of 1600 and
1700 other stock exchanges were opened including one in Belgium, Spain, Sweden and
Portugal. During that time, they did not have great impact on the people’s lives and economy
as a whole, mainly because there was only couple of them. So, the main purpose of stock
exchanges was trading of goods and currencies, but not in so high denominations. However,
in the year of 1785, the stock exchange in Amsterdam started doing trading with securities
and such way of trading was seen as a great innovation and opportunity to increase profit.
Founding this way of increasing profit was a reason for other European stock exchanges to
follow Amsterdam stock exchange regarding trading securities. It did not take much time for
stock markets worldwide (at that time, Canada and Australia stock markets) to start seeing
stock exchange as a one of the main ways to increase profit. Great importance of stock
exchanges led countries worldwide to open it up, so by 20th century stock markets in Asia,
Africa, and South America were opened.
When it comes to US stock exchange history, it can be traced back to over 200 years ago.
There were many reasons to start trading securities starting from personal needs, wants, as
well as willingness for profit increases to some more complex reasons such as financing war
by selling bonds.
The greatest and most important stock markets in the whole entire world, New York Stock
Exchange (NYSE) was found in 1817 playing key role in creating business and dictating
movement of whole business from year to year.
1.2 Definition, significance, and role of stock exchange:
By simple definition, stock exchange is an organized place for trading goods, bonds, shares,
financial derivatives…etc. Stock exchange also refers to the well-organized and wellregulated component of financial market where trading different types of securities such as
stocks and bonds, take place at price that is determined by the demand and supply forces. A
stock exchange or bourse can also be seen as a corporation providing opportunity and place to
its owners or financial market participants for increasing profit by trading company’s shares,
bonds, and other securities.
Besides this, stock exchanges also provide facilities for issue and redemption of securities and
other financial instruments, and capital events including the payment of income
and dividends. (Wikipedia, 2012)
Stock exchange certainly has many roles, but providing assistance when trading securities is
certainly among most important and most significant role of stock exchange. Not only
providing assistance is significant role of stock exchange, but also regulation, transparency
and control of buying and selling securities is role of stock exchange.
Many economists are opinion that the stock exchange perform important, if not key, role in
one nation’s economy since it provides investment opportunities by offering buyers and
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sellers places for trading securities, such as stock and bonds. Such investment opportunities
are ways for corporations, governments, and businesses to obtain funds necessary for
expanding business or financing publicly good project.
Stock exchanges also have one simple but significant role: providing direct job for people.
Providing direct job is expressed through different institutions including different brokerage
firms, investment banks…etc. It is well known that financial news organizations employ
hundreds of thousands of people across globe.
1.3 The role of the stock exchange in economy
Taking into consideration the fact that the stock exchange is an organized place for trading
goods, securities, financial derivatives…etc, whose daily volume is measured in billions of
dollars, it can be easily concluded that importance of it is huge.
In high developed countries, stock market is as important as other public goods.
Metaphorically speaking, it is like highways since both are used as an intermediate between
people’s needs, wants and wishes. Highways are associated with transferring goods,
people…etc, while stock markets are used for transferring securities such as stocks, bonds,
futures, options…etc. (Dedovic, 1998, p.1)
Based on this, we can say that trading or investing in securities seems to be one of the most
interesting modes of investment. Economic efficiency and well being of one country is
somehow connected with the efficiency and effectiveness of stock exchange. Such close
relationship is the reason why governments, corporations, central banks, and even individuals
in the countries around the world closely watch every activity on stock exchange. “Black
Monday” proved that the bulk of economical growth that took place in 20th was mainly
dependent on the stock exchanges mainly because if stock exchange activity was high, then
there was money and room for improvement. On the other side, if prices of stock went down
and activity was low, then recession was unavoidable. Corporations, governments, and small
industries simply realized that by stock market investment there is possibility for easy gains of
capital necessary for financing activities and easy way for increasing their wealth. Not only
corporations and governments see opportunity for increasing profit, but also individuals see
stock market as a way for increases in personal wealth. Nothing can change the fact that many
of the wealthy people have gained or increased their wealth thanks to investment in stocks and
bonds.
Also, nothing can change the fact that the whole global economy is dependent on those
biggest stock markets around the world where the most important one is the New York Stock
Exchange.
As it is well known, the NYSE is a huge market determining the parameters of the global
economy. In principle, when the New York Stock Exchange goes down, the whole global
economy will suffer and it will have a domino effect which can affect every stock market in
the whole world. In fact, this is what happened in the global crisis in 2008; as when the
mortgage crisis jumped into the stock market, this precipitated into a full scale stock market
crisis which caused a major crisis all around the world. In no time, there was a huge economic
in the global economy, as all stock markets around the world were effected immensely.
(Bestgrowthstock-blog, 2009)
In most simply way to explain why the stock exchange is important to economy is that when
stock prices go down, companies, ranging from the strongest one to the smallest one, feel the
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pain and vice versa. Economy is such science in which changes in one branch mostly leads to
changes in other branch. So, changes in stock prices will not affect only companies, but also
almost every individuals when most of them are not even aware of it. Mentioning this can
sound very pessimistic and negative regarding stock exchange in general. However, it also
shows the importance of stock exchange to economy since the stock exchange as a component
of stock market as the main dynamo of the economical forces, is a way for the real economy
to get strengthened.
Figure 1 - Importance and stock market development from 1950 – 2007

(Miskhin, Stanley, 2006, p.6)
As we can see from this figure, stock prices and stock market activities have been constantly
increasing from year to year. In last fifty year, there has been boosting of economy which is
mostly because increasing in stock market activities as well as prices.
However, people do not know much about that. In some sense it creates our destiny regarding
our future job orientation, since companies and corporations find stock exchange one of the
easiest ways to obtain money to finance their activities.
To make this more clear and convincible, let us assume a day when Wall Street marketers
announce that price of certain company’s share/stocks extremely went down by, say, 35%.
How would it affect companies and people? When it comes to people, fear of increases of
economic unemployment is the way to affect them. In many cases, loosing job means
increases in personal debt which further leads to insolvency or inability to repay all liabilities.
Further consequences are shown in loose of houses, discouragement, financial
dependence…etc.
On the other side, decrease in company share’s prices leads to financial dependence meaning
that companies facing that situation rely on money from bank. However, in order to obtain
funds from bank companies usually have to agree on repaying principal back with high
interest. Repaying principal back together with interest usually results in company’s less
profit which further leads to less innovation, less competition, economy of scale
decreases…etc. Besides that, borrowing funds from bank is heavily regulated.
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As Frederic S. Miskhin and Stanley G. Eakins mentioned in their book Financial Markets and
Institutions (2006, p. 49), stock exchange is an important factor in business investment
decision because of shares affecting the amount of funds that can be raised by selling newly
issued stock to finance investment spending.
In simply words, this means that a higher price for firm's share leads to a larger amount of
collected funds, which can further be used to finance different business activities starting from
inventory changes to rebuilding new company's facilities.
Well functioning economies are closely related to the well functioning of stock exchange also
for other reasons:
Channeling funds from savers to borrowers - channeling those funds results in promoting
economic efficiency as well as effectiveness in a way that competition increases, more funds
are provided, unemployment decreases, more projects being implemented…etc.
Figure 2 – participants being served by the stock exchange
Governments

Small business

Investors
STOCK EXCHANGE

Banks

Issuers

Intermediates
Corporations
-

-

More innovation – highly developed countries are associated with innovation because
people in those countries always want something new and sometimes have relatively
unrealistic wishes and demands.
Wealth of people is going up since they have job. This further leads to increases in
their spending and result in profit increases of firms.
Governments also benefit from this since people spend more, firms earn more, and
more taxes are collected.
Professor Mihailo Dedovic said in his book, “Stock Exchange” (page 2) that stock
exchanges is not only important because huge amount of capital being involved.

Far from being the case, it is mostly important because it is actually place where prices of
goods, services, capitals as well as money are determined.
What does this mean for us? In order to better understand consequences of stock exchange
activities regarding price determination, let us take a look on the picture below:

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Figure 3 – consequences of stock prices going down
NEW YORK STOCK EXCHANGE
Prices of corn are going up

NATIONAL ECONOMIES
Cash outflow is increased due to price increases

PEOPLE
People pay more money on corn products. It further leads to more money being saved since people can’t
afford to buy luxury goods. Final consequence is national economic activities are decreased.

Taking close look at this picture, we can notice that a New York Stock Exchange (NYSE)
activity determines not only activities of national economies, but also activities of people
being part of those economies. It is unwritten rule that when people have to pay necessities a
bit expensive than they used to, in order to keep their cash at balance, they will turn to
spending less money. This will further lead to less money on market and economic activities
will decrease.
Stock exchange helps company to raise fund which are necessary to expand its business. How
is that work? Let us take simple example.
Imagine yourself as an owner of small manufacturing company for producing furniture. You
have some amount of money which you use to buy a machine for production, found couple
customers, and you just started doing the business. So far, the business is doing well, profit is
increasing, you are winning new customers and soon you face situation in which you simply
can’t satisfy all customers’ needs and wants. Now you are facing the dilemma of whether to
increase the business and profit, but require you to spend certain amount of money or you can
just let your competitors take potential customers away from you, but you are letting
competition to increase and putting yourself in risky situation regarding your future business
and profit as well. In order to increase the business you have to buy new machines which
costs, say, $100,000. Now, you can wait for some period to earn more money to buy that
particular machine, but you are certainly going to lose many customers simply because they
can’t wait for you to make product. So, they will switch the company. You can also borrow
money from bank, but it is heavily regulated and soon you get money you will have to start
returning more money back than you borrowed because interest is included. Finally, you can
issue share which is third and most reasonable option to do. So, if I you do that, meaning if
you sell part of your company to third person, you will not be a 100% owner, but you will
have money necessary for financing your activity. In fact, third person is actually investor
having claims on company’s future earnings. At first sight it seems that you earn less amount
of money. Far from being the case, your business is increased and you earn more money.
So, all in all, whenever a company decides to expand business or strengthen its capital, it has
two main ways to obtain funds: either taking a loan from bank or issuing shares through the
stock market. However, the stock market is the primary source for company to raise funds
whenever it needs it.
-

There is saying that stock exchanges have predicted 3 out of last 10 recessions.
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One simple thing that can warn us that particular event can take place is lack of responsibility
regarding trading securities. This simply means that if, say, New York Stock Exchanges
(NYSE) does not run the business in a way it is supposed to or fully carry out its duty
regarding overseeing the stock trading process the investors will simply lose faith in the
transparency, fairness and safety of the New York Stock Exchange. If this happens then all of
the economic activity that the stock exchanges create will decrease and this will lead to an
overall drop in economic activity. The stock exchanges must be sure that investors are not
taken advantage of and that investors continue to have confidence in the system the stock
exchanges created.
-

It is mentioned previously that the stock exchange is closely related to the economy of
country in a way when stock prices go down; all participants in the economy, starting
from individuals to large corporations are affected. This is mainly because the
economy is such science in which changes in one branch affect and leads to changes in
other branch of the economy.

Figure 4 – domino effect

(Dreamstock, 2011)
The above picture explains effects of stock prices going down. As it is said, not only large
corporations and small business are affected, but also almost every individual even if many of
them are not aware of it. This is simply because of stock exchange, being one of the main
factors in the economy as well as acting as dynamo forcing changes in economy together with
well-full functioning stock market will help increase the strength of the reel economy.
All in all, the stock exchange is one of the most important sources for companies,
governments, small investors...etc, to gain money to expand business or finance their
activities. The stock exchange activity is actually our destiny creator in the sense that small
changes can cause positive or negative domino effect of worldwide business affecting directly
us as individuals. Watching news about changes in share prices during whole day, seven days
a week, it is just worth considering that stock exchange being part of our life.
People’s awareness, especially of those holding shares, about stock exchange activities should
be increased because the first impact they will see is fall or increase in their wealth. This can
also cause domino effect since fall in wealth leads to people being more hesitate to spend
money. Further effect is fall in consumer spending and list goes on.
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Taking into consideration everything mentioned, it is worth to say that nothing can change the
fact that well functioning stock exchange is a way to create new jobs and way to create
funding for companies from the biggest to those with small capacity. It is also fact that
without a proper functioning of stock exchange, it is not possible to have a healthy economy.
Simply saying, stock exchange is one of the key factors in producing high economic growth.
2. STOCK EXCHANGE IN BOSNIA AND HERZEGOVINA
When it comes to stock exchange of Bosnia and Herzegovina, where the most important one
is Sarajevo Stock Exchange (SASE), daily volume is measured in millions of dollars.
Likewise abroad, the company’s easiest way to obtain funds for financing its activities is
through issuing stock on the stock exchange. However, not all companies can participate on
the BH stock exchanges. In order to trade securities either on SASE or BLSE, the particular
company must be listed there. Sarajevo Stock Exchange (SASE) and Banja Luka Stock
Exchange (BLSE) set up certain requirements that company must fulfill in order to participate
on the stock exchange including: minimum amount of capital, minimum amount of size of
shares, minimum number of class holders, which is 150…etc.
As it is said previously, the way of trading of securities is being done on two stock exchanges
located in Sarajevo (SASE) and Banja Luka (BLSE). Both, SASE and BLSE aim to provide
quality and transparency as well as fair trading environment for their market participants
which include both domestic and foreign participants.
Over here in Bosnia and Herzegovina, business is done electronically meaning that brokers
are sitting at their offices and do the business through technology. So, we can’t see people
over here running around screaming and yelling like we see on American movies which
actually show real picture of American stock market’s working environment.
Stock exchanges in Bosnia and Herzegovina, with annual turnover measured in hundred
million of dollars, has been recording improvement in the way of working as well as profit
increasing from year to year. The performances of SASE and BLSE exploded especially in
last couple years. Such improvement and profit increases lead to capital market strengthening
which has also been recording improvement and people awareness from year to year.
Since 2002, market capitalization has risen by around twenty times and at the end of 2005 it
was EUR 3.3bn (Wiener Börse: EUR 107.1bn). The average daily trading volumes have also
soared in the past few years and hit EUR 1.5mn by 2005 (Wiener Börse: EUR 342mn). The
leading index of the Sarajevo Stock Exchange, the BIFX, rose from 1,264.20 at the end of
2002 to 4,045.57 points at the end of 2005. (SASE, 2011)
2.1 Criteria for Admission to the Admission to the Sock Exchange Listing
Stock exchange is not like ordinary market where everybody can enter in, do the business, and
walk away. Far from being the case, there are certain regulations and rules that particular
company must implement before participating in stock market. A particular company must
first be adjusted and registered by SEC (Security Exchange Commission) and even when it is
adjusted process is not done yet because the particular company must do two other
regulations: informing stock exchange about all circumstances affecting its business and
providing stock exchange with annual and semi-annual financial statement.
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Table 1 indicates that securities which complete the below listed criteria can be admitted to
the listing.
Table 1 – Criteria for admission of securities
The issuer is a joint stock company registered at the Commission
Years of business
Reality and objectivity of accounting reports

Capital
(the overall capital by the last balance sheet: capital stock,
paid surplus, reserve, assigned revenues or loss from previous
years, revaluation capital, undivided revenues or the business
year loss)
Minimum share size class
(considered to be the book4keeping value of share, i.e. market
capitalization, if a share already was a part of any segment of
the organized market
Share class percentage at the public offer
Share class owners

3
Revised
accounting
reports for the last three
business years
KM 4,000,000

KM 2,000,000

At least 25%
At least 150

Table 2 - Criteria for admission of bonds:

Years of business
Reality and objectivity of accounting reports

3
Revised
accounting
business report for the
last three years
KM 3,000,000

Overall nominal value of the sold bond series

Table 3 - Obligations after adjustment
Obligation after approval by security exchange commission:
- to inform stock market about all circumstances affecting its business
- to provide stock exchange with annual and semi-annual financial statement
2.2 Institutional framework of Sarajevo Stock Exchange
Well-functioning institutional framework in Bosnia and Herzegovina is necessary for
transparency. On the other side, transparency and fairness are closely related to attracting of
new investors while investors are considered to be most important players in a game called
capital market. Institutional framework can be divided into several segments:

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2.2.1 Security exchange commission
Security exchange commission is body established for the implementation of different tasks
which is mandatory for fair and transparent trading on Sarajevo Stock Exchange. Without this
body, it would not be possible to regulate and promote secure capital market in Bosnia and
Herzegovina. Another reason for the existing of security exchange commission is protection
of investors. Once we have body that works on behalf of investors in a way that protects their
rights, it is easy to attract new investors because they feel secure. Security Exchange
Commission aims to promote capital market of Bosnia and Herzegovina to foreign investors
and they found that building trust in the efficiency, effectiveness and fairness of the capital
market is key to succeed in it.
General overview of responsibilities of Security Commission of Federation of Bosnia and
Herzegovina (KOMVP,2011)
The Securities Commission of the Federation of Bosnia and Herzegovina has a broad
regulatory authority. Its responsibilities, among other, include the following:
 Regulation of conditions and methods of securities issuances and trading;
 Approval of issuances of shares and other securities by companies and banks;
 Approval of securities issuances bz fund management companies, investment funds,
mutual funds, and other legal persons that issue securities;
 Regulation of conditions and methods of bond issuance bz cantons and
municipalities;
 Prescribing the rules and control of securities trading based on securities exchange
and system of listing, using electronic means;
 Protection of investors' rights;
 Prescribing and enforcement of standards of disclosure to investors and the general
public on business operations of securities trading participants;
 Prescribing of conditions, issuing approvals of operations, and control of professional
intermediaries (e.g. brokers and dealers), and other securities trading participants.
2.2.2 Registry of Securities – in most simple way to explain Registry of Security is place
where data on securities are being held. Article 2 of the law defines that the Registry of
Securities performs operation of registration, safekeeping and maintenance of data on
securities, operation of transfers in accordance with the law regulating the issuance and
trading of securities. However, it is not only duty and operation preformed by the Registry of
Security. Far from being the case, it may also perform other operations including clearing and
settlement as well as all other operations that are related to clearing and settlement, but which
are firstly approved by Security Commission.
The Registry performs the following operations for securities issuers:





- registration, safekeeping, keeping and maintaining data on securities;
- making the list of shareholders and list of securities holders with the balance as of
the day requested by the issuer;
- making other reports as requested by the issuer;
- services in case of changes in property status and in case of changes in securities due
to conversion, securities denomination, merger and division of issuer's shares;
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo









- Services in exercising the rights derived from securities (submitting data to issuers
on securities holders, data on shareholders, who have the right to: vote in the shareholder
meeting, payment of dividend, as well as other rights) and other, in accordance with the
special contract.
The Registry performs the following operations for the securities holders:
- keeping the balance of securities on securities holders' accounts;
- transfer of securities based on applications for transfer of securities, submitted
directly to the Registry by the security holder;
- issuing certificates – statements and confirmations on balance of the securities
account;
- Services in connection with keeping, on separate accounts, the rights of third parties
on securities, of the lien, right of limitation of disposition, usufruct right and other rights;
Service in connection with joint stock company takeover and conducting tender offer
based on a special contract between securities holder and the Registry.

Figure 5 - Registration of data on securities
Registration of data on securities
Company’s management

Registry

Investor’s account

Account of issuer

Certificate showing the balance of the security account at the end of the month in which
a change on the balance of the account took place.

Confidence
Only investors and authorized person in the registry have access to the account.
As we can see on the picture above, registration of data of securities is a process consisted of
couple steps. First step is approving of security by security exchange commission. After it is
approved, it goes to the registry as a central place where securities are being held. In the
registry, two accounts are must be opened; account of issuer and account of investor. Such
accounts serve the purpose of providing certificate that shows the balance of the security
219

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

account at the end of the month. However, not all accounts are included, but only accounts in
which a change on the balance of the account took place.
2.2.3 Professional intermediates – brokerage firms
A brokerage firm or brokerage house refers to company which is allowed or licensed to buy
and sell securities. A brokerage firms are basically connection between buyers and sellers.
A brokerage firm is a financial institution that facilitates the buying and selling of financial
derivatives between a buyer and a seller.
Brokerage firms serve a clientele of investors who trade public stocks and other securities.
(Wikipedia, 2011)
Figure 6 – brokerage intermediation
Seller
Brokerage houses

Buyer

Being a connection or an intermediary between buyers and sellers, brokers as employers of
brokerage houses make, simply saying, clients’ wishes come true. In other words, it is brokers
who carry out client’s needs and wants when they decide to do trading on the stock
exchanges. So, brokers do trade but usually not on their own account and they are usually
paid on a commission basis.

-

Operations performed by brokerage houses on SASE
Buying and selling securities, not on their own account, but on the accounts of clients,
Buying and selling securities on their own account,(dealers)
They help in promotion of capital market,
Portfolio operating,
Investment consulting

2.2.4 Investment funds
Investment fund is actually firm that invests money which is collected from different
investors. Having money of many, small investors, investment fund invest it into in securities,
bonds…etc. By this, an investment company gives an opportunity to small investors to invest
their money in different securities. This becomes more important if we take into consideration
the fact that those investors would probably never have such an opportunity. Another benefit
of investing in investment funds is that small investors do not worry about trading costs which
can sometimes be high. It is investment fund, not small investors that can gain economies of
scale in while doing trading. There are two worldwide known investment companies:
open-end which includes mutual fund,
closed-end that includes investment trust

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2.2.5 Depositary banks
Over here in Bosnia and Herzegovina, depositary banks are actually commercial banks that
are allowed to do everything related to depositary job. Every brokerage house has its own
depositary bank which is used to make payments. In order to be allowed to perform
depositary job, a particular bank must be approved by Security Exchange Commission.
Depositary bank offers the following services:
-

-

Takes care of the fund property by keeping special accounts for property of each fund
and separating property of each fund from property of other funds
In case of mutual fund, repurchases and pays for shares, and makes payments to the
owners of share of mutual fund from fund profit
Takes care of the fact that calculation of net value of some stock or share in the fund
was made in accordance with the Law, other regulations, prospect and Statute of the
Fund
Reports the Company on corporative affairs referring to the fund property, which it is
taking care of, and executes the orders resulting from these affairs
Pays all incomes and other rights due for the fund benefit, which are resulting from its
property
Keeps business operations records performed as the bank depositor for each fund and
harmonizes it with Company’s records regularly
Provides auditors and other persons authorized for auditing, including the
Commission, with access to data and accounts related to the fund and its property
(BIB, 2011)

2.3 Comparison of SASE, in terms of annual turnover, with regional stock exchanges in
last two years.
Before comparing turnover of SASE with regional stock exchanges, we should first see
turnover of SASE from 2004 to 2010.
As the table below (Table 4) indicates, starting from 2004, activity on SASE, in terms of
money earned, had been constantly increasing till 2007 when the annual turnover was
1.274.340.116 KM. In 2008, in a year when recession started, activity on SASE dramatically
decreased by more than five hundred million of KM. Fall in activity was continued in the
following years and in 2010, annual turnover was the worst in SASE history with the amount
of 108.55.379 KM.
Based on this information we can divide turnover of SASE into two parts: prerecession
turnover and post recession turnover. It is noticeable that before recession there were constant
increases in turnover. However, when recession started, there have been constant decreases in
turnover.

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Table 4 - Turnover from 2004 to 2010
Year

Turnover

2010
2009
2008
2007
2006
2005
2004

108.554.379 KM
219.085.458,65 KM
477.079.376 KM
1.274.340.116 KM
654.717.252 KM
555.353.951 KM
231.137.332,00 KM

% percentage of total
turnover on B&amp;H stock
exchanges
38,38 %
62,32 %
63,43 %
94,48%
59,45%
66,51%
67%

The table below (Table 5) shows the comparison of Sarajevo Stock Exchange turnover for the
years of 2011 and 2010 with regional stock exchanges such as; Zagreb Stock Exchange,
Belgrade Stock Exchange and Ljubljana Stock Exchange. As it is noticeable, the Zagreb
Stock Exchange had the highest turnover followed by Belgrade Stock Exchange whose
turnover in last two years was over 400 million of euro. Capital market of Bosnia and
Herzegovina is less developed than those of Serbia and Croatia, which means that annual
turnover of SASE, is lesser with turnover around 100 million of euro in last two years. The
last one Ljubljana Stock Exchange with turnover less than 100 million of euro in last two
years. However, it does not mean that capital market in Slovenia is undeveloped. Far from
being the case, it is among most developed in Balkan region, but it is obvious that recession
took place in this country.
Table 5 – Comparison of SASE turnover with regional stock exchanges in last two years

Name of Stock exchange
Sarajevo Stock Exchange
Zagreb Stock Exchange
Belgrade Stock Exchange
Ljubljana Stock Exchange

Year
2011
2010
2011
2010
2011
2010
2011
2010

Turnover
/
55.668.921 EUR
749.911.956,6 EUR
934.882.913,7 EUR
208.180.538 EUR
222.475.934 EUR
19.352.083 EUR
20.485.947 EUR

Figure 7 – The graphical comparison of Sarajevo Stock Exchange with regional Stock
Exchanges.

222

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

As it is mentioned previously, the Zagreb Stock Exchange had the highest turnover in last two
years. Based on this information, it is noticeable that Croatia has highly developed capital
market, especially in last two years. According to this fact capital market in Bosnia and
Herzegovina is far from level at which capital market of Croatia is in terms of development.
Since, capital market over here is less developed; SASE should follow some strategies used
by Zagreb Stock Exchange.
3. CONCLUSION
Even if the facts, graphs, and tables shown in this work are promising, the general picture of
capital market in Bosnia and Herzegovina is still relatively unfavorable. The development of
BH capital market is related to the development of primary and secondary market. It means,
when primary and secondary markets are involved in high activity, the capital market is
strengthening. If we make comparison of capital market of Bosnia and Herzegovina with
those of developed countries, it is still underdeveloped and most of all, it is still seen as
relatively unimportant to the development of domestic country’s economy. One of the main
reasons for being less developed is corruption. This further means that there is legal
uncertainty which prevents investors to invest.
It is also important to realize that economic development of Bosnia and Herzegovina must
rely more on financial institutions as well as capital market. This means that the close
relationship between economic development and financial institutions must be established. So
far, stock exchange together with the banking industry is seen as a key factor in producing
high level of activity on capital market and economic growth as well. However, it is still far
from desired level of liquid financial market which is another problem of capital market of
Bosnia and Herzegovina being undeveloped.
Professor Mehmed Ganic is mentioning, in his work about current problems of capital market
(page 14) five reasons related to a possible recovery in the capital markets of B&amp;H which
must be taken into account.
First, the process of ownership concentration is largely complete, so the new owners
have lost an interest and incentive to appear in the capital market. It underestimates the shares
and leads to the discharge of the market. Consequently, this makes it unlikely to stimulate
interest both domestic and foreign investors;

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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Second, the benefits of financial markets resulting from the significant effects of
established organizational networks, as well as development and trading securities guided,
above all, the unit fixed costs. If the market is less liquid, then the trading costs are high. This
ultimately reduces the benefits of capital markets, discourage investors, in other words, a lack
of demand. In addition, B&amp;H has not yet fully adopted the culture of financing by issuing of
new shares which means that companies regularly resort to capital markets for the raising of
fresh capital.
Third, that the performance of capital markets is still under the influence of bad
political and economic environment of instability and poor social status of most citizens.
Fourth, there is a weak mobility of the issuers and limited of trading in securities of
the local companies, which resulted in the competitiveness of capital markets because of such
restrictions at a very low level.
Fifth, the capital market of B&amp;H is limited due to the slow and incomplete process of
privatization, following its further growth.
Another reason for capital market of Bosnia and Hezegovina being unfavorable and
underdeveloped is because of lack of foreign investors. Foreign direct investment plays an
important role in development of capital market in Bosnia and Herzegovina, so capital market
should be attractive in order to make foreigners invest their capital in Bosnia and
Herzegovina. However, capital market of Bosnia and Herzegovina is undeveloped, and it
means that investors do not want to take risk and invest. They usually turn to developed
financial markets which is key to encouraging them to take a risk and invest.
Capital marek of highly developed countries is characterised by various short-term financial
instruments. However, it is not case in Bosnia and Herzegovina.The overall financial market
should encourage all, starting from companies to financial institutions to governments to start
issuing short-term financial instruments.
By this time, banking industry and stock exchange are key for producing high level of activity
on capital marekt in Bosnia and Herzegovina. It is them which contribute to developing of
economy of B&amp;H.
All in all, capital market and stock exchanges as a segment of capital market play key role in
our daily lives and we should start seeing this of great importance. Being key of producing
high economic growh, government should attract investors by providing securtiy and
transparency while companies should be offering more diversified financial instruments in
order to attract customers.
Once again, nothing can change the fact that stock exchange plays key role in our daily lives
and it is stock exchanges that in some sense create our destiny.
REFERENCES
1. Internet: http://en.wikipedia.org/wiki/Stock_exchange, 05/01/2012
2. Dedovic, Dr.Mihailo, Berza – projekat od nacionalnog interesa, Pec, 1998
3. Internet: http://www.bestgrowthstock.com/blog/2009/04/02/the-importance-of-the-stockmarket-for-the-economy/ 28/12/2011
4. Internet: http://www.dreamstock.com, 03/10/2012
5. Mishkin, Frederic S. and Eakins, Stanley G. Financial Markets and Institutions, Fifth
edition, 2006
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6. Internet: http://en.wikipedia.org/wiki/Sarajevo Stock Exchange, 02/03/2012
7. Internet: http://en.wikipedia.org/wiki/Zagreb Stock Exchange, 02/03/2012
8. Internet: http://en.wikipedia.org/wiki/Belgrade Stock Exchange, 02/03/2012
9. Internet: http://en.wikipedia.org/wiki/Ljubljana Stock Exchange, 02/03/2012

Analyze and evaluate the system of internal control in the process of implementing the
audit of financial statements for business entities
Pece Nikoloski, Goce Menkinoski,
Facullty of economics in Prilep, Macedonia
E-mails: nikoloski.pece@yahoo.com,gocemenkinoski@yahoo.com
Abstract
Accounting is the process of recording, classifying, summarizing and reporting results from
financial transactions in the form of financial statements. Other than accounting, auditing the
financial statements determines whether those statements are prepared in accordance with
generally accepted accounting standards. You could say that this kind of audit users of
financial statements provides objective and independent opinion on the truthfulness and
honesty of the data presented in financial statements of business entities that are clients. The
application of the system of internal control in the implementation of the audit process is very
important activity in the process of making business decisions to allocate economic resources
and successful management. Information obtained from the analysis answer the question as
business entity in the previous period and how to function in the next period to be maintained
better result. The main goal of any business entity is achieving greater profits. The profit
maximization is striving to achieve the possible cost less and also the achievement of greater
revenues. Profit shown in the financial statements may differ from reality depending on the
way you handled the financial statements or in other words the way reports display where
they can be concealed large gains and losses. From there the auditors are those who weigh the
reality of participants' data in financial reports. How the financial result is a real set, so will be
better
and
better
decisions
are
made
based
on
such
information.
The advantages of better financial reporting are significant, hence the actions needed to
achieve those benefits are complex and comprehensive. As such we list the following
activities: improving the framework of financial reporting; strengthening of key factors
within the financial reporting; supporting the audit profession; supporting education, training
and public awareness. The main and primary objective of financial reporting is to provide
access balanced between financial reporting and audit requirements, which means meeting the
needs of different users of financial information but also will be complex to burden the
functioning
of
the
corporate
sector.
In practice, here are three main reasons to perform the process of analyzing financial
statements and financial indicators achieved:
1. The need for continuous monitoring of their own situation analyzes achieved financial
indicators, financial instruments are planned and implemented financial control;
225

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                <text>Economic efficiency and well being of one country is somehow connected with the efficiency  and effectiveness of stock exchange, so the main purpose of this study is to show and explain  workings of capital market in Bosnia and Herzegovina through activities of Sarajevo Stock  Exchange. The reason for this analysis is fact that the stock exchange performs important, if  not key, role in one nation’s economy since it provides investment opportunities by offering  buyers and seller’s places for trading securities, such as stocks and bonds. Such investment  opportunities are ways for corporations, governments, and business to obtain funds necessary  for expanding business or financing publicly good project.  Another reason for this analysis is the lack of awareness about importance of stock exchange  even if performance of stock exchanges affects everybody starting from individuals to large  corporations to governments.  Taking into consideration the fact that the stock exchange is an organized place for trading  goods, securities, financial derivatives…etc, whose daily volume is measured in billions of  dollars, it can be easily concluded that importance of it is huge.  This work is mentioning direct and indirect effects of stock exchange activity on an economy,  proving great impact on daily activity, not only of business, but of people as well.  This study also indicates that highly developed nations are closely related to the high level of  activity of stock exchanges and see stock exchange, as a component of capital market, a key  factor to success. Simply, stock exchange is as important as other public goods.  Less developed nations are not necessarily related to the poor performance of stock  exchanges; however, it could also be a main reason for those nations to be considered as a less  developed.  All in all, nothing can change the fact that stock exchange creates our destiny regarding our  future job orientation, since companies and corporations find stock exchange one of the  easiest ways to obtain money to finance their activities. Also, nothing can change the fact that  the whole global economy, including economy of Bosnia and Herzegovina is dependent on  those biggest stock exchanges around the world where the most important one is the New  York Stock Exchange.  This work is divided into three chapters. Section 1 deal with stock exchange and stock market  in general. When it comes to Section 2, it deals with stock exchanges in Bosnia and  Herzegovina, while Section 3 is actually conclusion part of this work.Keywords: stock exchange, capital market, security, stock, bond</text>
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                    <text>3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Physiological characterization of dunaliella sp. (chlorophyta, volvocales) from çamalti
saltworks (izmir-turkey)
Fatih Aksoy 1 Edis Koru2
1Katip Çelebi University Fisheries Faculty Dept. of Aquaculture, Çiğli,35620, İzmir,Turkey
2Ege University Fisheries Faculty Dept. of Aquaculture Bornova,35100, İzmir,Turkey
E-mail: fatih.aksoy@ikc.edu.tr
Abstract
Dunaliella (Cyanophyceae) microalgea is a species used for feeding live baits that are used in
larval fish production. Dunaliella species are intensively cultivated in algal biotechnology.
Because of the nutritional value and chemicals this microalgea contains, it is commonly used
in industries such as pharmacy, cosmetics and bait industry. From this point of view, it can be
said that this algea species has high economic value. It can be found in areas between ‰ 10
and ‰ 200 salinity content rate. In Turkey this species can be found in salinas near coasts and
salty-soft drink lakes. In this research, Dunaliella sp. species which is in Turkey’s
biggestmarine based saltworks “İzmir Çamaltı Saltworks” ecosystem isolated and cultivation
in controlled circumstances determined. As a part of this research, physicochemical
parameters such as optimum light, saltiness, density, biomass and pigment determined.
Keywords : Dunaliella sp., saltworks, microalgea, Çamaltı, Izmir, Turkey.
1.INTRODUCTION
Microalgae produce biomass and specific biomass in gredients from solar irradiation at high
degrees so that it is possible to produce economically feasible materials by microalgae.
The genus Dunaliella are wall-less eukoryatic algae and found in saline environments. They
are flagellate and consist of 23 species. They exhibit ideal growth at various salt
concentrations. In those conditions, their colours become orange-red (Massyuk, 1973).
Dunaliella belongs to the phylum Chlorophyta and family Polyblepharidaceae. It is
halotolerant and green (Avron and Ben-Amotz 1992; Garcia et al., 2007). It can live in
aquatic condition between 0,5-5 M NaCI salinities (Shariati &amp; Hadi, 2000, Phadwal &amp; Singh,
2003; Jahnke &amp; White, 2003). Dunaliella species produce some chemicals such as caretonids
(Hosseini Tafreshi &amp; Shariati, 2006; Hadi et al., 2008), glycerol (Hadi et al., 2008), vitamins
and proteins (Ghoshal et al., 2002) tough conditions (Hosseini Tafreshi &amp; Shariati, 2006;
Hadi et al., 2008). The reason how it can adapt in various salt concentrations is that it can
change the intracellular concentration of glycerol (Raja et al., 2007). People use glycerol in
automotive, leather, pharmaceutical, paint, cosmetic, food, pulp and paper, textile industries
and in the manufacture of microbial fermentation or it can be synthesized from petrochemical
raw materials. It can also be produced from soap manufacture of fats the amount of glycerol
produced in the world is 600,000 t/year (Wang et al., 2001; Taherzadeh et al., 2002).
Dunaliella species are consireded to be the most known microalgae in the autotrophic
production of glycerol (Borowitzka and Borowitzka, 1992). Dunaliella sp. are known as the
most halotolerant eukoryatic livings and they can adapt even to low salt saturated conditions
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such as 0.2%.On the other hand , it is the only eukoryatic photosynthetic organism found in
extremely concentrated saline lakes (Ben-Amotz and Avron, 1990).
Dunaliella salina, D. viridisare mostly found microalgae species in salty conditions (Davis,
1990). D. salina accumulates high amounts of β-caroten when there is a lack of nitrogen
sources or in high salinities and in high levels of irridance. β-caroten is a pigment and it is
added to health food products and is used as a food coloring agent (anti-cancer and
antioxidant agent) (Ben-Amotz and Avron, 1990).
2.MATERIALS AND METHODS
Dunaliella spp. were isolated from the Çamaltı solar saltworks and cultivated (Izmir, Turkey).
The Çamaltı Saltwork is the largest one in Turkey. It is in Izmir City which experiences
marine conditions. Its coordinates are 38°28’N and 26°50’E near the Izmir Bay. The reservoir
initial in the saltworks is found approximately in 2-3 inches depth of water. The density of
water is about 3 oBe – 5 oBe in November-May. Then, The water is pumped from the sea and
the salinity increases by 6-8 oBe. After that the density goes on increasing up to 22–24 oBe.
During this process micro algae appear and exhibit different colors. At higher concentrations
micro algae collapse. The temperature varied between 6-7°C (December), 4-5°C (February),
20-22 °C (April) to 28-30 °C (June), 38-40 °C (August) throughout the year.
We used single-cell isolation by micropipette.Dunaliella sp. were incubated and stored
without any process during two month. Then 1L flaks were incubated and reproduced. And
then different salinities determined growth parameters. Dunaliella sp. strain was cultivated at
four NaCI concentration (‰40,‰100) in 1L flaks. Laboratory’s temperature was 24±1 °C,
and lights were 1200 lux. Experiments were observed for 20 days. We used f/2 medium for
experiments.
Tablo 1. f/2 Medium (Guillard and Ryther 1962)
Component

Stock Solution

Quantity

Molar Concentration
in Final Medium

NaNO3

75 g/L dH2O

1 mL

8.82 x 10-4 M

NaH2PO4 H2O

5 g/L dH2O

1 mL

3.62 x 10-5 M

Na2SiO3 9H2O

30 g/L dH2O

1 mL

1.06 x 10-4 M

trace metal solution

1 mL

…..

vitamin solution

0,5 mL

…..

f/2 Trace Metal Solution
Component

114

Primary
Solution

Stock Quantity

Molar Concentration
in Final Medium

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

FeCl3 6H2O

....

3.15 g

1.17 x 10-5 M

Na2EDTA 2H2O

….

4.36 g

1.17 x 10-5 M

CuSO4 5H2O

9.8 g/L dH2O

1 mL

3.93 x 10-8 M

Na2MoO4 2H2O

6.3 g/L dH2O

1 mL

2.60 x 10-8 M

ZnSO4 7H2O

22.0 g/L dH2O

1 mL

7.65 x 10-8 M

CoCl2 6H2O

10.0 g/L dH2O

1 mL

4.20 x 10-8 M

MnCl2 4H2O

180.0 g/L dH2O

1 mL

9.10 x 10-7 M

f/2 Vitamin Solution
Component
thiamine
(vit.B1)

Primary
Solution
HCl ….

Stock Quantity

Molar Concentration
in Final Medium

200 mg

2.96 x 10-7 M

biotin (vit. H)

0.1 g/L dH2O

10 mL

2.05 x 10-9 M

cyanocobalamin
(vit.B12)

1.0 g/L dH2O

1 mL

3.69 x 10-10 M

For the extraction of chlorophlly-a, 5 ml of cultures incubated was taken daily from each
flask. Absorbance measurements were made by using a spectrophotometer. Algal growth was
monitored by counting number of cells in a counting chamber (Thoma Counting chamber).
3.RESULTS
Growth of Dunaliella sp. Çamaltı strain at different salinities is shown in Fig. 1. Maximum
cell density for Dunaliella sp. was obtained in 100 ‰ salinity and the lowest concentration
was found in 40 ‰ salinity.
Salinity clearly affected the cell density in Dunaliella sp. The optimum salinity for growth of
Dunaliella sp. strain was around 100 ‰ salinity.
A high salinity (100‰) was establisment chlorophlly-a (621,3 pg cell/1), a low salinity (40‰)
(347,1pg cell/1). (Fig.2)

115

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Cell density(x106 mL/1)

1800
1600
1400
1200
1000
800
600
400
200
0

40 ‰
100 ‰

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Cultivation time (day)

Fig. 1: Increase in cell density under the conditions of different salinities and 25 °C
temperatures
700
Chl a (pg cell/1

600
500
400
300

40 ‰

200

100 ‰

100
0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Cultivation time(day)

Fig. 2: Maximum chlorophyll-a concentration per cell in Dunaliella sp. grown at different
salinities

Optic density (680nm)

Optical density was directly proportional the density of the cell. It is shown that a high optical
density observed at high salinity. Increasing salinity caused optic density to increase (Fig. 3)
0.5
0.45
0.4
0.35
0.3
0.25
0.2
0.15
0.1
0.05
0

40 ‰
100 ‰

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Time (day)

116

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Fig. 3: The optical density of different salinities
At 40 ppt, culture had reached the logarithmic and stationary growth phase on day 3rd and
6th, respectively. On day 20, culture collapsed at 40 ppt. At 100 ppt, culture had reached the
logarithmic and stationary growth phase on day 5th and 9th. The highest physiological
development in Duneliella sp. was obtained at 100 ppt.
4.DISCUSSION
The Dunaliella species isolated from the solar saltworks on the Çamaltı Izmir differed
in their capacity for growth and physiological acclimation to varying culture conditions. In
the present study, the effect of salinity intensity, cell intensity, optic density, chlorophlly-a, on
growth of Dunaliella sp. Çamaltı strain was determined. It has been observed to grow
optimum at salinity around 100 ‰.
Gibor (1956), Jimenez and Niell (1990) reported that the optimum temperature for the growth
of Dunaliella viridis was around 30°C. Ak (2008) reported that the highest growth of D.
viridis of Çamaltı saltworks was found 25°C. Our study shown that the temperature was 25°C
the high salinity the best growing.
InthestudyheldbyDurmazet al in theyear of 2006, theyisolatedDunaliellasalinathecellsfrom
Konya
Salt
Lake
bythemethod
of
dilution.
Theymonitoredtheirgrowth
in
differentsalinities(0.62M, 0.85, 1.25 ve 1.71M). Inthisstudy, themostconvenient salt
concentrationwasobservedto be 1.71M NaCI. In1.71M NaCI, morecellnumberandhigher Bcarotenevalueswerefoundout.
Inthestudyheldby Dudu et al in 2001 on differentNaCI(10%, 15 %and20%)concentrations,
theyannouncedthattheyattainedthemostgrowthin NaCI10 % concentration.
REFERENCES
Ben-Amotz, A. and M. Avron, 1983. Accumulation of metabolites by halotolerant algae and
its industrial potential. Ann. Rev. Microbial., pp:95-119
Ben-Amotz, A., Avron, M., 1990. The biotechnology of cultivating the halotolerant alga
Dunaliella. Trends Biotechnol. 8, 121–126.
Ben-Amotz, A., Shaish, A., Avron, M., 1991. The biotechnology of
cultivating Dunaliella for production of b-carotene rich algae. Bioresour.
Technol. 38 (2–3), 233–235.
Borowitzka, M.A., Borowitzka, L.J., 1992. Micro-algal Biotechnology. Cambridge
University Press, Cambridge.
Davis, J.S., 1990. Biological Management fort he Productşon Salt from Seawater. In:
Introduction Applied Phycology, Akatsuka, I. (Ed.). SPB Academic Publishing, The Hague,
The Netherlands, pp:479-488.

117

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Dudu Evren, Ü., Ç. Kanlıtepe, C. Çıracı, G. Dönmez, 2001. Tuz Göl,’nden (Konya-Türkiye)
izole edilen Dunaliella türlerinin gliserol üretim kapasitesinin belirlenmesi. Ege Üniversitesi
Su Ürünleri Dergisi, 1. Alg Teknoloji Sempozyumu p, 225-232 (In Turkish).
Durmaz, Y., Gökpınar Ş., 2006. Dunaliella salina (Chlorophyceae) Büyümesi Üzerine
Tuzluluğun Etkileri. E.Ü. Su Ürünleri Dergisi, pp:121-124.
Garcia, F., Freile-Pelegrin, Y., Robledo, D., 2007. Physilogical characterization of Dunaliella
sp. (Chlorophyta, Volvocales) from Yucatan, Mexico. Bioresource Technology,pp:1359-1365
Javor, B., 1989. Hypersaline Enviroments: Microbiology and Biogeochemistry. 1st Edn.,
Springer-Verlag, New York, pp:328.
Lamers, P.P., Janssen, M., De Vos, C.H.R., Bino, J.R. and Wijffels, R.H. 2008. Exploring and
exploiting carotenoid accumulation in Dunaliella salina for cell-factory applications. Cell
Press, pp:631.
Kaçka, A., Dönmez, G., 2008. Isolation of Dunaliella spp. from a hypersaline lake and their
ability to accumulate glyserol. Bioresource Technology, pp.8348.
Massyuk, 1973. Morphology, taxonomy, ecology and geographic distribution
of the genus DunaliellaTeod. and prospects for its potential
utilisation. Kiev: Naukova Dumka. Massyuk. pp. 312.
Taherzadeh, M.J., Adler, L., Liden, G., 2002. Strategies for enhancing fermentative
production of glycerol-a review. Enzyme Microbiol. Technol. 31, 53–66.
Wang, Z.X., Zhuge, J., Fang, H., Prior, B.A., 2001. Glycerol production by microbial
fermentation: a review. Biotechnol. Adv. 19, 201–223.

Interactions between chemicals used in aquaculture and environment in terms of
sustainable development
Muhammet Altunok , Fatih Gülec , Zerife Peker , Klaus Kümmerer
Abstract
Aquaculture that is the farming of aquatic organisms such as fish, crustaceans, molluscs and
aquatic plants, is the fastest growing animal production sector in the world. Global production
from aquaculture for human consumption amounted to 73 million tonnes and the value of
US$ 110 billion in 2009 and comprised almost fifty percent of the world’s fish supply.
Aquaculture, thus, plays an important role in global efforts towards eliminating malnutrition
and brings significant health benefits by nutritional well-being. It significantly dominates
most devoloping countries in terms of contribution to development by increasing gross
domestic product, providing employment opportunities and improving incomes.
The potentially adverse impacts of aquaculture that is also threat the sustainability when the
sector grows unregulated or under poor management, is of considerable current environmental
118

�</text>
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                <text>Physiological characterization of dunaliella sp. (chlorophyta, volvocales) from çamalti  saltworks (izmir-turkey)</text>
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                <text>Fatih,  Aksoy</text>
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          <element elementId="94">
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            <description>A summary of the resource.</description>
            <elementTextContainer>
              <elementText elementTextId="17877">
                <text>Dunaliella (Cyanophyceae) microalgea is a species used for feeding live baits that are used in  larval fish production. Dunaliella species are intensively cultivated in algal biotechnology.  Because of the nutritional value and chemicals this microalgea contains, it is commonly used  in industries such as pharmacy, cosmetics and bait industry. From this point of view, it can be  said that this algea species has high economic value. It can be found in areas between ‰ 10  and ‰ 200 salinity content rate. In Turkey this species can be found in salinas near coasts and  salty-soft drink lakes. In this research, Dunaliella sp. species which is in Turkey’s  biggestmarine based saltworks “İzmir Çamaltı Saltworks” ecosystem isolated and cultivation  in controlled circumstances determined. As a part of this research, physicochemical  parameters such as optimum light, saltiness, density, biomass and pigment determined.  Keywords : Dunaliella sp., saltworks, microalgea, Çamaltı, Izmir, Turkey.  1.</text>
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                    <text>3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Importance of carrying capacity for sustainable trout culture: the case of “bagci balik”
Fatih Güleç1, Yusuf Güner1, Tolga Şahin2
1Ege Üniversitesi, Su Ürünleri Fakültesi, 35080 İzmir
2Çanakkale Onsekiz Mart Üniversitesi, Su Ürünleri Fakültesi, 17100 Çanakkale
Abstract
Trout is a fish species that is produced widely all over the world and it has an important place
in the aquaculture industry. For the sustainable development of the trout culture, it is need to
determine how much fish can be stocked per unit volume (carrying capacity). Over-stocking
could threaten fish welfare and sustainable use of environmental resources. Estimating of
carrying capacity is necessary both to minimize these adverse impacts on fish and
environment, and in terms of sustainable use of water resources. In this study, carrying
capacity of a commercial land based trout farm in Koycegiz (Mugla/TURKEY) was
investigated. Two methods were used to calculate carrying capacity and compared with each
other: “Oxygen requirement based carrying capacity” and “Metabolic waste based carrying
capacity”. The mean values of studied water quality parameters that used in calculations were
measured as; 14.12 ± 0.72 °C for temperature, 7.78 ±0.20 for pH, 10.1 ± 0.93 mg/l for
oxygen, 3,05 ± 0,84 m3 s-1 for flow rate. The annual production capacity was calculated
with these measured parameters. By the observed data, the carrying capacities of the farm
were found respectively with based on oxygen requirement as 538.42 ± 196.22 tonnes; and
based on metabolic waste as 1113.4 ± 463.7 tonnes per years. According to the results that
obtained from the study, there was significant difference between two carrying capacity
estimation methods. However when the oxygen levels of the water were examined, in some
months the negative values, that could adversely affect the life and growth of the trouts, were
observed. Fort this reason, to reduce the limiting effect of the oxygen and reach maximum
carrying capacity; it is needed to be integrated oxygenation or aeration systems into the
production ponds. On the other hand, for the sustainable use of the environmental sources in
the facilities engaged in production according to the metabolic waste based carrying capacity,
it is important to use advanced water filtration systems.
Keywords: Carrying capacity, land-based trout culture, water quality.
1.INTRODUCTION
Aquaculture is a farming method of aquatic animals and plants for nutrition, stocks
enrichment, making ornaments, hobby activities, and for scientific studies in a controlled or
semi-controlled manner, by human (Çelikkale, et al., 1999). Today, it is a rapidly growing
industry especially in terms of animal food. The combined result of development in
aquaculture worldwide and the expansion in global population is that the average annual per
capita supply of food fish from aquaculture for human consumption has increased by ten
times, from 0.7 kg in 1970 to 7.8 kg in 2008, at an average rate of 6.6 percent per year(FAO,
2010). According to data obtained from the FAO, in 2010, total global aquaculture
64

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

production (quantity) is 73 million tonnes. The value of the world aquaculture harvest,
excluding aquatic plants, is estimated at US$110.1 billion in 2009 (FAO, 2010). Turkey’s
total fisheries production is 653 thousand tons and 167 thousand tons of total production
comes from aquaculture. More than half of the amount of aquaculture (51%) is due to the
trout culture, and the value of production is around US$ 207 million (TUIK, 2010).
Sustainability has great importance for the aquculture industry as in all sectors. At this point,
the condition of the water is one of the vital criteria for aquaculture enterprises that are at the
top level of the production cycle. Aquaculture can be made depending on the the physical,
chemical and biological properties of water. These criteria should be in optimum standards
fort he sustainable aquaculture. Rate of flow and quality of incoming water are the first
parameters to be dealt with in establishing suitable living conditions for fish. (Penneli and
Mclean, 1996). Because, the water that is used for aquaculture, both brings oxygen to the
aquaculture environment and also provides removal of the accumulated wastes (Balık, et al.,
2002). On the other hand, estimating the carrying capacity of the farm, is an important criteria
to be considered. Carrying capacity is usually expressed in terms of quantity of fish per unit of
water flow (loading rate) or per cubic meter of rearing space (density) (Hinshaw, 2000).
Carrying capacity has an important influence on the success of aquaculture operations. The
appropriate stocking level is ascertained in carrying capacity studies (Frechette, 2005). Many
studies have demonstrated an effect of stocking density on various aspects of the welfare of
farmed fish (Wedemeyer, 1997). In intensive aquaculture fishes are usually reared at high
densities, which has led to concerns about welfare of the fish, so there is a need for the
development of reliable stocking density guidelines (Adams, et al., 2007; Ashley, 2007; Ellis,
et al., 2002; Huntingford, et al., 2006). Several causative mechanisms have been proposed to
explain why high densities negatively affect growth and feed utilization. The biochemical,
behavioural, and physiological changes induced by high stress levels are presumed to be
energetically costly, affecting the amount of energy available for growth (Barton and Iwama,
1991; Pickering, 1992; Vijayan and Leatherland, 1998; Wendelaar, 1997). High rearing
density in itself may further reduce access to feed, thereby reducing feed intake and directly
affecting growth (Alanara and Brannas, 1996; Boujard et al., 2002; Marchand and Boisclair,
1998). Incorrect Carrying capacity aplications both affect negatively to fish welfare and cause
environmental issues. This is the biggest obstacle in front of the goal of sustainable
aquaculture. Therefore, it is necessary to estimate the carrying capacity so good to reflect the
facts.
In this study, carrying capacity of a commercial land based trout farm in Koycegiz
(Mugla/TURKEY) was investigated. For the sustainable use of Yuvarlak Çay, the optimum
capacity and the maximum capacity had been calculated with both formulas.
2.Materials and methods
2.1.Experimental area
The study was conducted in a commercial trout farm that was located on Yuvarlakçay Stream
in Fethiye/Koycegiz. The commercial farm aimed to produce 900 tons trout per year in its
project. For the sustainable trout farming on this stream continuously, optimum and maximum
amount of fish, that could be stocked in the farm, was estimated.

65

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

2.2.Fish material
The farmed fish species in the trout enterprise, was rainbow trout (Oncorhynchus mykiss)
(Walbaum, 1792), and the production period that was used in the farm, from egg to market
size.
Method
A number of different formulas have been devised to calculate carrying capacities, taking into
account oxygen consumption, growth rate of fish, feeding rates, water volume and
temperature, and other factors. Dissolved oxygen and ammonia (un-ionized) concentrations
are the primary limiting factors in the culture systems, with oxygen normally the more critical
(Hinshaw, 2000). Therefore carrying capacity (stock density) of the fish farms, can be
estimated with the amount of oxygen consumed by fish or methabolic wastes of the fish. To
be able to make this calculation, it is important to know amount of the feed given to fish.
Because, the single artificial input is the feed to the fish that grown in the farm and to the
natural environment (Balık, et al., 2002). In the calculations; two criteria are taken into
consideration: The oxygen rate that fish need to metabolize the feed and the amount of
ammonia released by digestion of feed the environment. In this study the approach of
Brannon, (1991), calculating carrying capacity of trout farming with oxygen and metabolic
wastes based methods.
2.3.Oxygen-Based Carrying Capacity Estimation
The optimum carrying capacity according to water flow rate and the dissolved oxygen in the
water was calculated by the following formula:
N = (0,25) / ( 0,00143 x Of)
p=R/N
*N

: L /min required/kg of fish feed

*0,25

: kg O2 to metabolize 1 kg of fish feed

*0,00143

: conversion constant

*Of

: inlet oxygen minus outflow oxygen (Oa - Ob)

*p

: kg of food fed

*R

: total rate of flow in L / min.

Mechabolic waste-Based Carrying capacity Estimation
The maximum amount of fish that could be produced by increasing the amount of oxygen in
the water, was calculated with this method.

66

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Table 1. NH3 percentage of Total Ammonia Relationship to pH and Temperature
Temp
(°C)

pH
6.0

6.5

7.0

7.5

8.0

8.5

4

0.01

0.03

0.12

0.37

1.10

3.39

8

0.02

0.05

0.16

0.50

1.58

4.82

12

0.02

0.07

0.21

0.68

2.12

6.40

16

0.03

0.09

0.29

0.92

2.86

8.52

20

0.04

0.13

0.40

1.24

3.83

11.18

N = (0,032 x r) / (0,00143 x 0,02)
p =R/N
*N

: L / min. required/kg of food fed

*0,032

: kg NH3 + NH4+ produced/kg of food fed

*r
: % NH3 of total ammonia present (Table 1. NH3 percentage of Total
Ammonia Relationship to pH and Temperature(Brannon, 1991).
*0,00143

: conversion constant

*0,02

: ppm max. NH3

*p

: kg of food fed

*R

: total rate of flow in L/min

To use in the calculations, temperature(°C), flow rate (L/s), oxygen (mg/L) and the other
chemical water parameters were measured regularly every month.
Table 2. Monthly measured water parameters used in the calculations
Parameters

Ma
y

Jun
e

July Aug
.

Sep
.

Oct. Nov
.

Dec
.

Jan.

Feb
.

Mar
.

Apr
.

Flow Rate 2,88 2,38 2,3
(m3/s)
4

1,94

1,9
8

2,7
4

2,91

3,09

4,1
3

4,0
9

4,05

4,04

Temperatur
e (°C)

14,3

14,
5

14

13,7

13,5

12,
5

13,
7

14,2

14,2

67

14,7 15,3 14,
8

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

pH

8,1

NH3 (%)

7,9

8,1

7,6

7,4

7,6

7,8

7,8

7,7

7,8

7,8

7,8

3,20 2,09 3,2
3

0,98

0,6
5

0,9
8

1,49

1,55

1,1
5

1,5
5

1,94

1,94

Oxygen
(mg/l)

9,3

9,3

8,9

9,9

10,
0

11,
7

10,3

10,4

11,
0

9,5

9,3

11,6

Oxygen
(mg/l)

4,0

4,2

5,7

5,12

3,8
5

4,9
7

4,97

6,35

8,0
1

6,3

6,51

7,77

(discharged
from ponds)

3.Results and Discussion
3.1.Carrying Capacity
According to “Oxygen-Based Carrying Capacity Estimation” method, it was calculated
between May to April with the varying flow rate in the ongrowing ponds, respectively; 212.5
, 175.6 , 156.6 , 163.1 , 169.8, 315.0 , 264.7 , 295.3 , 406.0, 315.8 , 298.8 , 457.7 tons trout
could be farmed in the months (Table 3. The total amount of fish produced by month (tons)
The sample enterprise could make two production in a year. When the carrying capacity was
calculated; 198,77± 24,58 tons trout could be farmed in the one production period and in the
second period; 339,72 ± 30,64 tons. It was estimated that totally 538,49±55,22 tons of trout
could be produced per year.
According to the calculation of “Mechabolic waste-Based Carrying Capacity Estimation”
method, that is taken into consideration the percentage of toxic NH3 in the total ammonia;
respectively; 241.3, 305.3, 194.2, 529.5, 816.8, 747.9, 520.5, 534.6, 966.3, 707.6, 559.1,
557.7 tons fish could be farmed (Table 3. The total amount of fish produced by month (tons).
472,50 ± 109,01 tons trout could be farmed in the one production period and 640,97 ± 70,65
tons in the second period. It was estimated that totally 1113,47 ± 179,98 tons of trout could
be produced per year.
Table 3. The total amount of fish produced by month (tons)
May June July
Capacity
(tonnes/ye
ar)

68

Aug
.

Sep.

Oct.

Nov
.

Dec. Jan.

Feb.

Mar
.

Apr.

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

Based on 212,
oxygen
5

175,
6

156,
6

163,
1

169,
8

315

264,
7

295,
3

406

315,
8

298,
8

457,
7

Based on 241,
ammonia
3

305,
3

194,
2

529,
5

816,
8

747,
9

520,
5

534,
6

966,
3

707,
6

559,
1

557,
7

Figure 1. Estimated carrying capacities calculated with oxygen based and ammonia based
methods between May to April.
4.CONCLUSION
One of the most important issues is to measure water properties. While doing this the physical
and chemical parameters should be known. Beveridge (2004), previously reported that any
increase in temperature affected the metabolism, oxygen consumption and also increased
activity of fish. Optimal on-growing temperature level was reported between 10-15 °C by
Sedgwick, (1985) , and in addition to this between 12-18 °C by Çelikkale, (1988). In this
study, the water temperature ranged between 12,5-15,3 °C and the average temperature level
in the ponds was 14,12 ± 0,21 °C (Table 2). The temperature level did not adversely affect the
development of the fish.
Çelikkale, 1988 reported that 100 – 150 kg rainbow trout could be farmed with 1 L.s-1 . In
this study the avarage flow rate was measured 3.05 ± 0.24 m3.s-1. Additionally, water flow
rate was reduced during the experimental period due to very low rainfall in that season.
The dissolved oxygen (DO) level in the water is another important criteria in the estimating of
carrying capacity of the trout farm. Çelikkale, (1988); Edwards, (1987); and Stevenson,
(1984); reported that the DO level had to be more than 6 mg.L-1 for the rainbow trout
farming. The levels of DO varied between 8,9 and 11.7 mg.L-1 at farm inlet during the study
and the lowest DO at the pond outlet was observed in September (3.85 mg.L-1) (Table 2).
69

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

This value was found to be below the limit of DO concentration for rainbow trout farming (5
mg.L-1) as recommended by Belaud, (1995). Additional oxygenation systems were used to
solve lower DO problem, in the farm.
The present study showed that carrying capacity (stock density) of a trout farm was related
with water parameters. Although, the presence of suitable water temperature for trout farming,
the water parameters limited production. In the first method, oxygen was the significant
limited parameters for carrying capacity. Especially between May and September, the oxygen
levels decreased due to reduced water flow and as a result of this, amounts of the production
in those months were minimum. Although this farm was allowed to produce 900 tons of trout,
maximum 538,49±55,22 tons of rainbow trout could be farmed due to the low oxygene levels.
The problem of low oxygen levels for carrying capacity can be solved with by adding
alternative oxygenation systems. Aerators were used in this farm but it was observed that the
aerators were not sufficient and suitable capacity. Toxicity of total ammonia in water, was the
most important parameter that limiting carrying capacity. By using additional oxygenation
systems, the carrying capacities were increased between May and September. But maximum
carrying capacity was determinated by using the second method. By using the second
calculation method, the maximum production and carrying capacity was observed 1113,47 ±
179,98 tons per year.
REFERENCES
Adams, C. E., Turnbull, J. F., Bell, A., Bron, J. E., and Huntingford, F. A. (2007) Multiple
determinants of welfare in farmed fish: stocking density, disturbance, and aggression in
Atlantic salmon(Salmo salar). Canadian Journal of Fisheries and Aquatic Sciences(64), 336–
344.
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Buhan, E., Koçer, M., Polat, F., Dogan, H., Dirim, S., and Neary, E. T. (2010) Evaluation of
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Wendelaar, B. S. (1997) The stress response in fish. Physiological Reviews, pp. 591-625.
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Photon Systems Instruments (2011) FluorCam Instruction Manual, http://www.psi.cz

The Effect Of Water Temperature On Spawning Out Of Season In Rainbow Trout,
Oncorhynchus Mykiss
Yusuf Güner1, Halit Bayrak2, Fatih Güleç1
1Ege Üniversitesi, Su Ürünleri Fakültesi, 35080 İzmir
2SuleymanDemirel University, Mehmet Süreyya Demiraslan M.Y.O, 32510 Isparta
Abstract
Salmonid culture is a production sector with widespread throughout the world. Different
stocks of rainbow trout spawn in certain months of the year in hatcheries. This seasonality of
spawning imposes considerable constraints on trout farming because the consequent
restrictions on the supply of eggs and fry make it difficult for on-growing farm to maintain a
continuity of production of table-size fish throughout the year. Hatcheries should artificially
control the spawning time of their broodfish so that batches of eggs and fry might be
produced all year round. The modification of spawning time are possible using manipulation
of photoperiod. This series of studies were carried out in order to identify the effects of
varying water temperature on the ovulation of rainbow trout be exposed to photoperiodic
manipulation. This study was carried out in two different facilities. In the first facility, the
photoperiod regime was only consisted of constant short days (LD 6:18). The water
temperature had been changed between 7.5 and 15 °C during the the experiment in this
facility. In the second facility, it had been changed between 11 – 25.5 °C. The broodstock was
seperated into two different group. These photoperioded regims consist of constant short
days(LD 6:18) and altered photoperiod (LD 18:6 from February until May, then LD 12:12
from May until June, then LD 6:18 from June until November) which are acted to advanced
spawning. In both experiments, photoperiod regimes changed the maturation time regardless
of water temperature. However, when the water temperature is high, modulating effects of the
temperature on the maturation and ovulation time were observed. When the spawning time
modified to June – August, ovary growth and egg survival rate had been reduced by the
temperature effect ( &gt;14 °C). It was found that the egg quality decreased when the water
temperature was 17 °C although the eggs could been removed from the fish. Based on the
72

�</text>
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                <text>Trout is a fish species that is produced widely all over the world and it has an important place  in the aquaculture industry. For the sustainable development of the trout culture, it is need to  determine how much fish can be stocked per unit volume (carrying capacity). Over-stocking  could threaten fish welfare and sustainable use of environmental resources. Estimating of  carrying capacity is necessary both to minimize these adverse impacts on fish and  environment, and in terms of sustainable use of water resources. In this study, carrying  capacity of a commercial land based trout farm in Koycegiz (Mugla/TURKEY) was  investigated. Two methods were used to calculate carrying capacity and compared with each  other: “Oxygen requirement based carrying capacity” and “Metabolic waste based carrying  capacity”. The mean values of studied water quality parameters that used in calculations were  measured as; 14.12 ± 0.72 °C for temperature, 7.78 ±0.20 for pH, 10.1 ± 0.93 mg/l for  oxygen, 3,05 ± 0,84 m3 s-1 for flow rate. The annual production capacity was calculated  with these measured parameters. By the observed data, the carrying capacities of the farm  were found respectively with based on oxygen requirement as 538.42 ± 196.22 tonnes; and  based on metabolic waste as 1113.4 ± 463.7 tonnes per years. According to the results that  obtained from the study, there was significant difference between two carrying capacity  estimation methods. However when the oxygen levels of the water were examined, in some  months the negative values, that could adversely affect the life and growth of the trouts, were  observed. Fort this reason, to reduce the limiting effect of the oxygen and reach maximum  carrying capacity; it is needed to be integrated oxygenation or aeration systems into the  production ponds. On the other hand, for the sustainable use of the environmental sources in  the facilities engaged in production according to the metabolic waste based carrying capacity,  it is important to use advanced water filtration systems.  Keywords: Carrying capacity, land-based trout culture, water quality.</text>
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                    <text>3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

The Analysis of Sustainability Development of Eastern and South Eastern Europe in the
Post Socialist Period
Fatih Çelebioğlu
Dumlupınar University, Faculty of Economics and Administrative Sciences,
Department of Economics, Kütahya, TURKEY,
fcelebi@dumlupinar.edu.tr
Phone: +90 274 265 20 31 (ext.2114-2200)

Fax: +90 274 265 21 97

Abstract
Since the collapse of socialism, Eastern Europe and South Eastern (Balkans) Europe countries
have been expeditiously changing as social, economic and politic structure. Some former socialist
countries (as Bulgaria, Slovenia, Slovakia, Latvia, Lithuania, Poland and Romania) and Greece
became full member of European Union. Some Balkan countries (Serbia, Montenegro, Croatia,
Bosnia-Herzegovina, and Macedonia) lived difficult war years. After the wars, they have started
to struggle for economic, social and political reconstruction process. Some CIS (Commonwealth
of Independent States) countries (as Moldova, Ukraine and Belarus) tried to adapt market
economics. Each country in this region wants bigger real per capita income, better welfare level,
and generally become a developed country. But these countries have some political, economic
and social problems in development and sustainability process. The aim of this paper is to
analysis the countries in terms of development indicators such as per capita GDP growth rates,
Human Development Index values and Sustainable Human Development Index values in the
period of 2000-2010. It will be used the tools of spatial statistics (ESDA -Exploratory Spatial
Data Analysis).
Keywords: Sustainable Development, per capita GDP growth, Human Development Index,
Sustainable Human Development Index, ESDA (Exploratory Spatial Data Analysis)
1. INTRODUCTION
Eastern and South Eastern Europe are an important area because of witness historic and politic
experiences and incidents for ages. But it has been also living historical alteration in recent
decades. Although some Balkan countries were relatively stable in 1990s, there was war in
Balkan Peninsula. Some former socialist countries (as Bulgaria, Slovenia, Slovakia, Latvia,
Lithuania, Poland and Romania) and Greece became full member of European Union. The others
have been struggling for this aim. In spite of Kosovo declared of independence in 2008, many
countries haven’t been accepting this situation. Besides, CIS (Commonwealth of Independent
States) countries (as Moldova, Ukraine and Belarus) tried to adapt market economics.
Nevertheless these regions of Europe are living relatively stable condition nowadays, compare
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�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

with last ten years. Whole countries in these regions, especially gain independence in recent
decades, wants to become rapidly developed country.
After a long war and unstable political period, the countries have taken an opportunity about their
development process nowadays. These regions have been gaining stable structure overtime and
this stable period has been supporting development indicators. In this paper, Balkan countries are
being analyzed in terms of development indicators such as average rates of per capita GDP
growth in the period of 2000-2010, Human Development Index in 2010, and Sustainable Human
Development Index in 2010.
2. HUMAN DEVELOPMENT
DEVELOPMENT INDEX (SHDI)

INDEX

(HDI)

AND

SUSTAINABLE

HUMAN

UNDP calculates The Human Development Index (HDI). HDI includes some special data such as
life expectancy at birth, adult literacy rates, gross primary-secondary and tertiary enrolment, GDP
(gross domestic product) per capita (PPP - purchasing power parity- US$). HDI separates three
subgroup as developed (high development), developing (middle development), and
underdeveloped (low development) countries.
Africa, Middle East, South Asia and some South American countries have big problems in terms
of level of human development. Especially in Africa, the level of human development is lower
than other regions of the world.
Besides, The Sustainable Human Development Index (SHDI) was calculated by the Ministry of
Environment and Natural Resources of Sri Lanka in 2008. The new version of the index has been
revised in 2010. This index calculated with the contributions of United Nations University in
Tokyo, Japan. The index calculated by incorporating environmental aspects and quality of the life
such as carbon emission, bio capacity, ecological footprint and poverty to Human Development
Index (HDI) developed by UNDP to consider the sustainable human development aspects which
ignore in HDI. (environmentmin.gov.lk, April 12, 2012).
Acoording to Togtokh and Gaffney (April 15, 2012); by including carbon emissions in
recalculation of the HDI, it has gotten an indication of the cost of one country’s quality of life to
another’s. If a country has a very high HDI but also high carbon emissions, it can be said that the
high quality of life enjoyed by this nation comes at a price to the quality of life in other countries,
particularly developing nations, and to future generations. The index shows that, while the US is
vilified for its poor record on tackling emissions, Canada is a long way from being a role model.
Yet, with the traditional HDI, the UN is using such countries with very high human development
as examples to the rest of the world. Other nations attempting to emulate the success of these
particular countries in terms of human development look at the economic model and policies
adopted to bring about that success.
Sustainable Human Development Index (SHDI) has been calculating by using HDI and different
indexes as the following:
SHDI = ¼(HDI +Ecological Index-Climate Change Index +Poverty Index)

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3. DATA, METHODOLOGY AND FINDINGS
3.1. Data
GDP (Gross Domestic Product) per capita growth (annual %) data set comes from World
Development Online Database (WDI online) for 21 countries in the period of 2000-2010. Human
Development Index data (2010) has been prepared by UNDP (United Nations Development
Programme). Sustainable Human Development Index was calculated by Ministry of Environment
and Natural Resources of Sri Lanka in 2008. The last version of the index was published in
2010.
To analyze spatial relations between Eastern and South Eastern Europe countries, we use GeoDa
(Geographic Data Analysis) software package which conducts Spatial Data Analysis, geovisualization, spatial autocorrelation and spatial modeling78.
3.2. Methodology
3.2.1. Quartile Maps
Our analysis start with the quartile maps of the distribution of our variables for each country.
Darker colors explain higher values and lighter colors show lower values in quartile map in for
all variables.

Figure 1: Per capita GDP annual growth
rates in the period of 2000-2010

Figure 2: Human Development Index
Values (2010)

Figure 3: Sustainable Human
Development Index (SHDI) values in
2010
78 Here are some of the studies in this regard: Rey and Montouri (1999), Ying (2000), Manfred et al.
(2001), Le Gallo and Ertur (2003), Van Oort and Artezema (2004), Dall’erba (2005), Voss et al. (2006),
Ezcurra et al. (2007), Ezcurra et al. (2008), Battisti and Di Vaio (2008), Celebioglu and Dall’erba (2010).
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Figure 1 shows that per capita GDP growth rate is
especially high values in Albania, Bulgaria, Belarus,
Lithuania, Moldova, and Ukraine. On the contrary,
Macedonia, Greece, Turkey, Hungary, and Slovenia
have the lowest values in this analysis. Figure 2
displays distribution of each country. According to
figure 2, especially Greece, Czech Republic, Hungary,
Slovenia, Slovakia, and Estonia have the highest
Human
Development
Index
values.
Bosnia
Herzegovina, Macedonia, Turkey, Moldova, and
Ukraine have the lowest HDI figures. Figure 3 presents
Sustainable Human Development Index (SHDI)
values. In this figure, we can see only one difference
compare with Figure 2 in terms of highest values. While Estonia is one of the highest value
countries in HDI, there is Lithuania in SHDI in place of Estonia. We understand from quartile
maps that disparity is clear in this region. For this reason we start ESDA analysis with spatial
weight matrix.
3.2.2. Spatial Weight Matrix
A spatial weight matrix is the necessary tool to impose a neighborhood structure on a spatial
dataset. As usual in the spatial statistics literature, neighbors are defined by a binary relationship
(0 for non-neighbors, 1 for neighbors). Weight matrix calculation is performed under GeoDa. It
can be used two basic approaches for defining neighborhood: contiguity (shared borders) and
distance. Contiguity-based weights matrices include rook and queen. Areas are neighbors under
the rook criterion if they share a common border, not vertices. Distance-based weights matrices
include distance bands and k nearest neighbors. Based on these two concepts, we decided to
create weight matrices to investigate the distribution of our variables of interest: k_3 nearest
neighbor matrix. Due to space constraints, we present the k_3 nearest neighbor matrix only
below:
 w (k )  0 if i  j
 ij
*

 wij (k )  1 if dij  Di (k ) and wij (k )  wij (k ) /  wij (k ) for k  3
j

 w (k )  0 if d  D (k )
ij
i
 ij

(1)

where di,j is great circle distance between centroids of country i and j and Di(k) is the 3th order
smallest distance between regions i and j such that each region i has exactly 3 neighbors. Now
that the weight matrix has been defined, we estimate a couple of spatial statistics that will shed
some light on the spatial distribution of our variables. The most common of them is Moran’s I
which is a measure of global spatial autocorrelation (Anselin, 1988).
3.2.3. Calculation of Moran’s I for Global Spatial Autocorrelation
Spatial autocorrelation refers to the correlation of a variable with itself in space. It can be positive
(when high values correlate with high neighboring values or when low values correlate with low
neighboring values) or negative (spatial outliers for high-low or low-high values). Note that
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positive spatial autocorrelation can be associated with a small negative value (e.g., -0.01) since
the mean in finite samples is not centered on 1. Spatial autocorrelation analysis includes tests and
visualization of both global (test for clustering) and local (test for clusters) Moran’s I statistic
(Anselin et al. 2006).
Global spatial autocorrelation is a measure of overall clustering and it is measured here by
Moran's I. It captures the extent of overall clustering that exists in a dataset. It is assessed by
means of a test of a null hypothesis of random location. Rejection of this null hypothesis suggests
a spatial pattern or spatial structure, which provides more insights about a data distribution that
what a quartile map. For each variable, it measures the degree of linear association between its
value at one location and the spatially weighted average of neighboring values (Anselin et al.
2007; Anselin 1995) and is formulized as follows:
n

It 

n

*

 wij (k ) xit x jt
i 1 j 1
n n

(2)

xit x jt

i 1 j 1

&amp;
w
ij
Where
is the (row-standardized) degree of connection between the spatial units i and j and
Xi,j is the variable of interest in country i at year t (measured as a deviation from the mean value
for that year). Values of I larger (smaller) than the expected value E(I) = -1/(n-1) indicate positive
(negative) spatial autocorrelation. In our study, this value is (-0.050). There are different ways to
draw inference here. The approach we use is a permutation approach with 999 permutations. It
means that 999 re-sampled datasets were automatically created for which the I statistics are
computed. The value obtained for the actual dataset has then been compared to the empirical
distribution obtained from these re-sampled datasets.

The results of Moran’s I are presented in table 1 below. All the results indicate a positive spatial
autocorrelation, i.e. the value of a variable in one location depends positively on the value of the
same variable in neighboring locations. For instance, when the per capita income in one country
increases by 1%, the one of its neighbors increases by slightly more than 33%. All of our three
variables of interest are significant (at 1%) with the k_3 nearest neighbor matrix. For this reason,
this is the weight matrix we will use in the rest of our study.
Table: The results of Moran’s I for the nearest four neighbors
Variables
K_3
Per capita GDP (average values of 2000-2010) 0.3310
(0.010)
Human Development Index Values (2010)
0.2421
(0.029)
Sustainable Human Development Index Values 0.2672
(2010)
(0.027)
Note: p-values are into brackets
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3.2.4. Moran’s Scatterplots of Variables
The Moran scatter plot complements Moran’s I because it provides to categorize the nature of
spatial autocorrelation into four types: low-low (LL), low-high (LH), high-low (HL) and highhigh (HH). The x-axis captures the value of a variable compared to the average value of the
sample. For example, all the points on the right hand side of the figure mean (the vertical axis in
the middle) that in the corresponding provinces, the value of the variable under study was above
the sample’s average. On the other hand, the y-axis captures the average value of the same
variable in the neighboring locations (with the neighbors being defined by the weight matrix). For
instance, all the points below the mean (the horizontal axis in the middle of the figure) represent
provinces of which neighbors display, on average, a lower value than the sample’s mean.
The result of this approach is a figure with four windows which reflect the correlation between
the relative (to the mean) value of a variable in one location and the relative value of the same
variable in neighboring locations. For instance, the quadrant HH means a high value in the
studied area and a high value in the neighboring areas. Countries located in quadrants I and III
refer to positive spatial autocorrelation, i.e. the spatial clustering of similar values, whereas
quadrants II and IV represent negative spatial autocorrelation, i.e. the spatial clustering of
dissimilar values. Note also that the link between a scatter plot and Moran’s I is reflected by a
line of which slope is the value of Moran’s I statistic.
Figure 4: Moran’s Scatterplot for
average of per capita GDP growth in
the period of 2000-2010

Figure 5: Moran’s
Scatterplot for Human
Development Index in 2010

Figure. 6: Moran’s
Scatterplot for Sustainable
Human Development
Index in 2010

Figures 4 to 6 above show the Moran scatter plots of our variables of interest. All of the per
capita GDP, Human Development Index and Sustainable Human Development Index have
positive spatial autocorrelation that is reflected by the value of Moran’s I and the fact that most of
the countries are located in quadrants HH and LL.
When we look at the HH quadrant in scotterplot of per capita GDP growth, we see mostly
Eastern Europe such as Belarus, Estonia, Latvia, Lithuania, Moldova, Romania, and Ukraine. In
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the contrary, the HH quadrant in scotterplot of HDI has been composed by Croatia, Czech Rep.,
Poland, Slovakia, Slovenia, Estonia, Latvia, and Lithuania.
Besides, Czech Rep., Poland, Slovakia, Slovenia, Estonia, Latvia, and Lithuania take part in HH
quadrant of SHDI.
3.2.5. LISA Statistics for Local Spatial Autocorrelation
LISA statistics (Local Indicators of Spatial Association) measure, by definition, the presence of
spatial autocorrelation for each of the location of our sample. It captures the presence or absence
of significant spatial clusters or outliers for each location. Combined with the classification into
four types defined in the Moran scatter plot above, LISA statistics indicates significant local
clusters (high–high or low–low) or local spatial outliers (high–low or low–high). The average of
the Local Moran statistics is proportional to the Global Moran's I value (Anselin 1995; Anselin et
al. 2007).
Anselin (1995) formulated the local Moran’s statistics for each country (I) and year (t) as follows:

 x 
I i   i   wij x j
 m0  j

with m0   xi2 / n

(3)

i

where wij is the elements of the row-standardized weights matrix W and xi(xj) is the observation
in country i(j). Their significance level is based on a randomization approach with 999
permutations of the neighboring provinces for each observation.
Figure 7: LISA Cluster Map of per
capita GDP growth (average of
2000-2010)

Figure 8: LISA Cluster Map of HDI
(2010)

The randomization approach is used in the context of a numeric permutation approach to describe
the computation of pseudo significance levels for global and local spatial autocorrelation
483

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

statistics. In order to determine how likely it would be to observe the actual spatial distribution at
hand, the actual values are randomly reshuffled over space 999 times.

Figure 9: LISA Cluster Map of
SHDI (2010)

Countries that are in HH area (red color) in Figure
7 are Belarus, Estonia, Latvia, Lithuania, and
Ukraine. HH type autocorrelation is very strong in
Eastern Europe. In Figure 8 is LISA cluster map of
HDI. This figure point out that Czech Republic,
Poland and Slovakia are in HH quadrant (red
color), Romania is in HL area (pink color) and
Ukraine is in LL area (blue). It is interesting that
although Ukraine is in HH quadrant of per capita
GDP growth, there is a different situation in the
figure. Though Figure 9 is similar to Figure 8,
Albania is in LL area of SHDI. But in LISA map
of SHDI, Ukraine was in HH type autocorrelation.

4. CONCLUSIONS
The aim of this paper is to analysis spatial distribution of development indicators such as per
capita GDP growth rates, Human Development Index values and Sustainable Human
Development Index values in the period of 2000-2010. For this purpose we use quartile maps,
Moran’s Scotterplots and LISA (Local Indicators of Spatial Association) statistics.
We investigate spatial distribution of per capita GDP growth in the period of 2000-2010, Human
Development Index (2010) and Sustainable Human Development Index (2010). First of all, our
quartile maps show that there is an important development level gap between countries of
Europe. Secondly, when we estimate spatial autocorrelation by means of Moran’s I, our results
indicate positive (and significant) global autocorrelation for all of our variables and thus
indicating the geographical location of a country influences its level of per capita GDP growth
rate, Human Development Index and Sustainable Human Development Index.
Secondly, these results are corroborated by the corresponding Moran’s Scatterplots that display
the HH quadrant in scotterplot of per capita GDP growth includes mostly Eastern Europe such as
Belarus, Estonia, Latvia, Lithuania, Moldova, Romania, and Ukraine. In the contrary, the HH
quadrant in scotterplot of HDI has been composed by Croatia, Czech Rep., Poland, Slovakia,
Slovenia, Estonia, Latvia, and Lithuania. Besides, Czech Rep., Poland, Slovakia, Slovenia,
Estonia, Latvia, and Lithuania take part in HH quadrant of SHDI.
Thirdly, LISA statistics confirm the significant presence of local spatial autocorrelation and
highlight spatial heterogeneity in the form of two distinct spatial clusters of high and low values
484

�3rd International Symposium on Sustainable Development, May 31 - June 01 2012, Sarajevo

of per capita GDP growth rate, Human Development Index and Sustainable Human Development
Index.
And finally, we can say that there is an important spatial heterogeneity and spatial disparity in
terms of our all variables. But the countries in the east part of Europe are divided two groups in
the region. Firstly, swiftly growing countries in terms of per capita GDP growth rate and
secondly, countries that has high level of SHDI –HDI. For this reason, we can say that swiftly
growing countries in terms of per capita GDP growth rate should be developed also in terms of
human development.
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The Factors Determined To The Improvement In The Least Developed And Developing
Countries: Testing A Model
Gözde Ergin, Adil Oğuzhan
Trakya University, Department of Econometrics.

Abstract
Finding the different ways of the improvement as a multidimensional process causes
different improvement ways in all countries in the world. The economic improvement that cause
a structural changing is very important in all economies all over the world and it is necessary for
the least developed countries at the same time. These countries have solved the phenomena of
poverty, unemployment, low life standards and unimproved. The differentiation in the sociocultural structures of the least developed and developing countries effect the improvement in a
positive way.
In the study, the socio-economic factors of improvement and a classification according to
the gross national product levels per person in the least developed and developing countries have
been done by taking the definition accepted by World Bank into consideration. There are fifteen
countries in the classification of the least developed and developing countries. The data of thirtythree factors in the comparison of these countries have been obtained from the data source of
World Bank, OECD, EUROSTAT and UN (2000 – 2009).
486

�</text>
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                <text>The Analysis of Sustainability Development of Eastern and South Eastern Europe in the  Post Socialist Period</text>
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            <name>Author</name>
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                <text>Fatih , Çelebioğlu</text>
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                <text>Since the collapse of socialism, Eastern Europe and South Eastern (Balkans) Europe countries  have been expeditiously changing as social, economic and politic structure. Some former socialist  countries (as Bulgaria, Slovenia, Slovakia, Latvia, Lithuania, Poland and Romania) and Greece  became full member of European Union. Some Balkan countries (Serbia, Montenegro, Croatia,  Bosnia-Herzegovina, and Macedonia) lived difficult war years. After the wars, they have started  to struggle for economic, social and political reconstruction process. Some CIS (Commonwealth  of Independent States) countries (as Moldova, Ukraine and Belarus) tried to adapt market  economics. Each country in this region wants bigger real per capita income, better welfare level,  and generally become a developed country. But these countries have some political, economic  and social problems in development and sustainability process. The aim of this paper is to  analysis the countries in terms of development indicators such as per capita GDP growth rates,  Human Development Index values and Sustainable Human Development Index values in the  period of 2000-2010. It will be used the tools of spatial statistics (ESDA -Exploratory Spatial  Data Analysis).  Keywords: Sustainable Development, per capita GDP growth, Human Development Index,  Sustainable Human Development Index, ESDA (Exploratory Spatial Data Analysis)</text>
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