Respected readers,
Journal of Natural Sciences and Engineering (JONSAE) is a peer-reviewed biannual journal
that aims at the publication and dissemination of original research articles on the latest
developments in the fundamental theory, practice and application of engineering, science, and
technology. We provide a platform for researchers, academicians, professionals, practitioners,
and students to impart and share knowledge in the form of high-quality empirical and
theoretical research papers. The journal covers all areas of genetics and bioengineering,
electrical and electronics engineering, information technology, architecture, applied
mathematics, computer sciences, and civil engineering.

In this second issue in 2022, I would like to express my gratitude to our authors and
manuscript reviewers for their unselfish effort. You have continued your commitment and
diligence to the Journal in helping us to produce quality and meaningful content that has the
opportunity to advance the field. All of us had personal and professional challenges due to
return to in-class teaching and dynamic experiences we have all witnessed in terms of changes
in how the higher education functions in the post-pandemic period, and yet despite this, we
managed to assure continuous publication of our Journal. Thank you all for being part of this
wonderful academic endeavor.
This issue is comprised of three articles. A review article we are presenting you stands at the
crossroad of biochemistry and molecular biology and deals with the current state of knowledge
related to the hydrophobic interaction chromatography as an experimental method. Two
original papers come from two different areas; one is dedicated to machine learning and
another to solar irradiation.

In the end, we plan to build upon the excellent editorial infrastructure in the next years. In
addition to managing the normal turnover of the Editorial Board members, we will seek to
expand it by recruiting additional members who can provide expertise in areas that are not
currently well represented. In particular, we hope to recruit board members with expertise in
such areas as statistical and biostatistical methods, computer science, bioengineering, and
biomedical engineering, among others. We also hope to leverage the expertise of the Editorial
Board members to train the next generation of scholars and potential Editorial Board members
by finding ways to pair student reviewers with senior reviewers for a peer-review mentorship
as a part of building a better research environment for new scientists. A similar approach is
planned for the administrative members of the Editorial Board, with the aim of improving the
quality of the overall Journal design and acquiring a modern visual identity.

Having in mind all stated, I wholeheartedly invite you to read this issue and join our team.

Yours sincerely,
Adna Ašić, PhD
Editor in Chief
]]> Hydrophobic Interaction Chromatography: A Key Method for Protein Separation Nermana Kovačević, Mohammad Tariq AL-Boush
Literature review
(pg. 2 - 13)
Solar Irradiation Prediction Based on M5 Model Tree and Feature Importance Evaluation
Lejla Idrizović, Lejla Lulić Skopljak, Faris Haznadarević, Haris Ahmetović
Original research
(pg. 14 - 25)
Letter Recognition Using Machine Learning Algorithms
Merima Ćeranić, Samed Jukić
Original research
(pg. 26 - 37)
]]>
Journal of Natural Sciences and Engineering (JONSAE) is a peer-reviewed biannual journal that aims at the publication and dissemination of original research articles on the latest developments in the fundamental theory, practice and application of engineering, science, and technology.

We provide a platform for researchers, academicians, professionals, practitioners, and students to impart and share knowledge in the form of high-quality empirical and theoretical research papers. The journal covers all areas of Genetics and Bioengineering, Electrical and Electronics Engineering, Information Technology, Architecture, Applied Mathematics, Computer Sciences, and Civil Engineering.

In this issue, I would like to express my gratitude to our authors and manuscript reviewers for their unselfish effort. You have continued your commitment and diligence to the Journal in helping us to produce quality and meaningful content that has the opportunity to advance the field. All of us had personal and professional challenges due to the pandemic and yet despite this, we managed to produce quality content and improve JONSAE’s indexation and future perspectives in terms of its metrics and quality growth. Thank you all for being part of this wonderful academic endeavor. We have both original research and literature review articles published in this issue. Our readers can learn details about how data technology and engineering ventures can contribute to medicine in the form of predictions for depression and anxiety as well as how to use machine learning-based gene clustering in brain cancer classification. Also, you can find out what is the potential of biomaterial-based solutions in cancer research treatment and can homicides be solved using the classification method. In the end, you can read about a staple gene in pharmacogenomics research, CYP4F2*3, and the effect of its polymorphism on different traits, including optimum anticoagulation therapy administration based on the patient’s genetics.
In the end, we plan to build upon the excellent editorial infrastructure in the next years. In addition to managing the normal turnover of the Editorial Board members, we will seek to expand it by recruiting additional members who can provide expertise in areas that are not currently well represented. In particular, we hope to recruit board members with expertise in such areas as statistical and biostatistical methods, computer science, bioengineering, and biomedical engineering, among others. We also hope to leverage the expertise of the Editorial Board members to train the next generation of scholars and potential Editorial Board members by finding ways to pair student reviewers with senior reviewers for a peer-review mentorship as a part of building a better research environment for new scientists. A similar approach is planned for the administrative members of the Editorial Board, with the aim of improving the quality of the
overall Journal design and acquiring a modern visual identity.

Having in mind all stated, I wholeheartedly invite you to read this issue and join our team.

Yours sincerely,
Adna Ašić, PhD
Editor in Chief]]> The Potential of Biomaterial-Based Solutions in Cancer Research and Treatment Hannah Boone
Literature review
(pg. 4 -11)
Analysis of High School Graduate Data Using Database Analytics Tools
Ezana Ćeman, Samed Jukić
Original research
(pg. 12-26)
Frequency Locked Loop in Estimating Active, Reactive and Apparent Powers Meris Lihić, Slobodan Lubura
Original research
(pg. 27-42)
Prediction of Solved Homicides Using Classification Method
Lamija Zukić, Samed Jukić
Original research
(pg. 43-49)
Machine Learning-Based Gene Clustering on Brain Cancer Using K-Means and Hierarchical Clustering Methods
Fatih Yilmaz
Original research
(pg. 50-61)
Recommendation Engine on IPTV
Vedad Njuhović
Original research
(pg. 62-69)
Depression and Anxiety Analysis and Prediction using Big Data Technologies Mersiha Ćeranić
Original research
(pg. 70-83)
Prevalence of rs2108622 (CYP4F2*3) Single Nucleotide Polymorphism – A Review
Selma Žiga, Hana Efendić, Larisa Bešić
Literature review
(pg.84-92)
]]> Journal of Natural Sciences and Engineering (JONSAE) is a peer-reviewed biannual journal
that aims at the publication and dissemination of original research articles on the latest
developments in the fundamental theory, practice and application of engineering, science, and
technology. We provide a platform for researchers, academicians, professionals, practitioners,
and students to impart and share knowledge in the form of high-quality empirical and
theoretical research papers. The journal covers all areas of genetics and bioengineering,
electrical and electronics engineering, information technology, architecture, applied
mathematics, computer sciences, and civil engineering.

In this first issue in 2022, I would like to express my gratitude to our authors and manuscript
reviewers for their unselfish effort. You have continued your commitment and diligence to the
Journal in helping us to produce quality and meaningful content that has the opportunity to
advance the field. All of us had personal and professional challenges due to the pandemic and
return to in-class teaching and yet despite this, we managed to assure continuous publication
of our Journal. Thank you all for being part of this wonderful academic endeavor.
This issue is dedicated to natural sciences only, whereby we have two original research articles
and two review articles. Original articles are dealing with testing results for the patients in the
Canton Sarajevo, one related to biochemical blood analyses and another to cancer genetics.
When it comes to literature reviews, we are changing the pace and are pointing our interest
towards plants; the reviews are dealing with plants in medicine and environmental science.

In the end, we plan to build upon the excellent editorial infrastructure in the next years. In
addition to managing the normal turnover of the Editorial Board members, we will seek to
expand it by recruiting additional members who can provide expertise in areas that are not
currently well represented. In particular, we hope to recruit board members with expertise in
such areas as statistical and biostatistical methods, computer science, bioengineering, and
biomedical engineering, among others. We also hope to leverage the expertise of the Editorial
Board members to train the next generation of scholars and potential Editorial Board members
by finding ways to pair student reviewers with senior reviewers for a peer-review mentorship
as a part of building a better research environment for new scientists. A similar approach is
planned for the administrative members of the Editorial Board, with the aim of improving the
quality of the overall Journal design and acquiring a modern visual identity.

Having in mind all stated, I wholeheartedly invite you to read this issue and join our team.

Yours sincerely,
Adna Ašić
Editor in Chief]]> Phytoremediation: A Green Approach to Fight Heavy Metal Contamination in the Soil Jasmin Novalic
Literature review
(pg. 2 - 11)
The Presence of Bioactive Compounds in Plants of the Amaranthaceae Family and Their Use in Medicine: A Review
Lejla Kadric
Literature review
(pg. 12 - 31)
Comparison Test of Sensitivity Between Next Generation Sequencing (NGS) Hotspot Panel and Droplet Digital PCR of KRAS G12 / G13 Mutation
Azra Hajdarevic, Lana Salihefendic, Rijad Konjhodzic, Adis Kandic, Ivana Ceko, Dino Pecar, Muhamed Curic, Amina Kurtovic – Kozaric
Original research
(pg. 32 - 41)
The Assessment of the General Health Status of the Sarajevo Canton Residents Through General Biochemical Testing Results
Faris Ramic
Original research
(pg. 42 - 55) ]]>